FastQCFastQC Report
Fri 27 May 2016
SRR2138714_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138714_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3560410
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39850.047.9805071
GTATCAA72650.033.2111661
TCAACGC83900.028.3139934
ATCAACG85150.027.8983443
CAACGCA86400.027.5426255
AACGCAG87650.027.1970466
TATCAAC91050.026.408722
ACGCAGA103000.023.0635347
CGCAGAG107150.022.1316418
GTGGTAT21950.020.9285761
GTACATG96150.020.230181
TACATGG97600.019.420792
TGGTATC21800.019.363992
GCAGAGT123400.018.9489219
ACATGGG97600.018.699933
GAGTACT71400.018.28767412-13
CAGAGTA119250.017.66487710-11
CATGGGG73300.017.5028554
AGAGTAC110400.016.88794910-11
GTACTTT83250.015.76046114-15
Produced by FastQC (version 0.11.5)