##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138714_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3560410
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.93434744874888	31.0	31.0	34.0	30.0	34.0
2	32.182030440314456	34.0	31.0	34.0	30.0	34.0
3	32.1055215551018	34.0	31.0	34.0	30.0	34.0
4	35.474119553646915	37.0	35.0	37.0	34.0	37.0
5	35.279466971500476	37.0	35.0	37.0	33.0	37.0
6	35.816009111310215	37.0	35.0	37.0	33.0	37.0
7	35.73965414095568	37.0	35.0	37.0	33.0	37.0
8	35.70559851253086	37.0	35.0	37.0	33.0	37.0
9	37.197123645872246	39.0	37.0	39.0	34.0	39.0
10-11	37.08756028097888	39.0	37.0	39.0	33.0	39.0
12-13	37.03759552972832	39.0	37.0	39.0	33.0	39.0
14-15	38.06817529441834	39.0	37.5	41.0	33.0	41.0
16-17	38.0077132970641	39.0	37.0	41.0	33.0	41.0
18-19	38.022025553236844	39.0	37.5	41.0	33.5	41.0
20-21	38.01857809769584	39.0	37.0	41.0	33.5	41.0
22-23	37.95736058642502	39.0	37.0	41.0	33.0	41.0
24-25	37.88180110707772	39.0	37.0	41.0	33.0	41.0
26-27	37.71726270320278	39.0	37.0	41.0	33.0	41.0
28-29	37.565099338381486	39.0	37.0	41.0	33.0	41.0
30-31	37.43591601383865	39.0	37.0	40.0	32.0	41.0
32-33	37.278311215688774	38.0	36.5	40.0	32.0	41.0
34-35	37.16695453592774	38.0	36.0	40.0	31.5	41.0
36-37	37.23719891263535	38.0	36.0	40.0	32.0	41.0
38-39	37.21815801479892	38.0	36.0	40.0	31.5	41.0
40-41	37.180339313682964	38.0	36.0	40.0	31.0	41.0
42-43	37.256845049056054	38.0	36.5	40.5	32.0	41.0
44-45	37.27463057928086	39.0	36.5	41.0	32.0	41.0
46-47	37.16415630439816	38.0	36.0	40.5	31.0	41.0
48-49	37.10931134711909	38.0	36.0	40.0	31.0	41.0
50-51	37.03342013145251	38.0	36.0	40.0	31.0	41.0
52-53	36.90627789686949	38.0	36.0	40.0	31.0	41.0
54-55	36.753613510376326	38.0	35.0	40.0	31.0	41.0
56-57	36.63774175997932	38.0	35.0	40.0	31.0	41.0
58-59	36.42509048411476	38.0	35.0	40.0	30.0	41.0
60-61	36.28613441267048	38.0	35.0	40.0	30.0	41.0
62-63	36.08059763169928	38.0	35.0	40.0	30.0	41.0
64-65	35.81411485147209	38.0	34.0	39.5	29.0	41.0
66-67	35.52482411674651	37.0	34.0	39.0	29.0	41.0
68-69	35.323338170809436	37.0	34.0	39.0	29.0	40.5
70-71	35.050090574058544	36.5	34.0	38.5	29.0	40.0
72-73	34.78830348906594	36.0	34.0	38.0	28.5	40.0
74-75	34.547867210497515	35.5	33.5	38.0	28.0	39.5
76-77	33.38052795178643	34.5	31.5	37.0	26.5	38.5
78-79	34.03097353044901	35.0	33.0	37.0	28.5	39.0
80-81	34.050100327975734	35.0	33.0	37.0	29.0	38.5
82-83	33.841522131647764	35.0	33.0	37.0	29.0	38.0
84-85	33.65017635538923	35.0	33.0	36.0	29.0	38.0
86-87	33.49153968744123	35.0	33.0	36.0	28.5	38.0
88-89	33.38973158372367	35.0	33.0	36.0	29.0	38.0
90-91	33.254827047744996	35.0	33.0	35.5	28.0	38.0
92-93	33.25533548945137	35.0	33.0	35.0	29.0	38.0
94-95	33.182581564678166	35.0	32.5	35.0	29.0	37.0
96-97	33.28119280298039	35.0	33.0	35.0	29.0	37.0
98-99	33.38092717358589	35.0	33.0	35.0	29.0	37.0
100	32.40698199109972	34.0	31.0	35.0	26.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	3.0
17	7.0
18	19.0
19	35.0
20	103.0
21	324.0
22	1096.0
23	3165.0
24	7349.0
25	15189.0
26	26363.0
27	39964.0
28	56412.0
29	74088.0
30	93053.0
31	115376.0
32	142582.0
33	178212.0
34	231160.0
35	309805.0
36	457648.0
37	854592.0
38	639392.0
39	304595.0
40	9878.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.613578211498115	20.43629806679568	13.843349501883209	24.106774219823
2	15.464174069840272	21.134054785825228	40.03311416381821	23.36865698051629
3	18.63407227486288	25.516373404396347	30.287961379707045	25.561592941033723
4	12.99004889886277	17.50739381138689	38.104656486191196	31.397900803559143
5	13.316809019185992	37.73276111459074	35.550400094371156	13.400029771852118
6	31.011821672223146	37.47613336666283	18.056993436149206	13.455051524964823
7	27.133026814327565	32.242803497350025	22.711204608458015	17.9129650798644
8	24.913057765819104	36.57780985897692	20.706154628259103	17.802977746944872
9	25.415443726986496	16.46658671332796	21.208989975873564	36.90897958381198
10-11	24.53686513631857	26.65612949070472	28.59375746051719	20.21324791245952
12-13	24.989355158535112	24.07166590364593	28.949910263143853	21.989068674675107
14-15	22.100642903485834	25.65061327206698	26.366064582449773	25.88267924199741
16-17	21.899064995323574	28.719094149269324	27.173022208116482	22.20881864729062
18-19	21.866434330676018	28.02624509143876	28.72790146841851	21.379419109466706
20-21	22.569412015850357	27.15173211410402	28.76156983966367	21.517286030381953
22-23	22.283472297778182	27.08090951446088	28.560617137819833	22.075001049941097
24-25	22.08752325209109	27.204072053841877	28.651858474438047	22.056546219628988
26-27	22.08293647999557	27.345208225204416	28.574143025169953	21.997712269630064
28-29	22.077456493748052	27.440903436408476	28.54517850098134	21.936461568862132
30-31	22.295486265081422	27.311155786369373	28.427095030772776	21.966262917776426
32-33	22.04484345212892	27.45284412553206	28.428840862464472	22.073471559874548
34-35	22.223969980132242	27.501112424291378	28.3713116403511	21.903605955225277
36-37	22.209323561565075	27.397581484506837	28.34152791623775	22.051567037690333
38-39	22.044087112368757	27.58843897126245	28.399122964357517	21.968350952011285
40-41	22.328115682788404	27.445027954436952	28.241176566723365	21.985679796051276
42-43	22.197541967170125	27.51739209259797	28.04198810028919	22.24307783994272
44-45	22.242114287229374	27.61255939877977	27.965372684073614	22.179953629917247
46-47	22.296620171961496	27.618641336178108	27.73693859445817	22.347799897402226
48-49	22.26876657938784	27.687558866521826	27.685917305695675	22.35775724839466
50-51	22.254236614986965	27.804462074255703	27.606963619899094	22.334337690858234
52-53	22.435418741011173	27.71892785583459	27.516179887155655	22.329473515998576
54-55	22.392528285216372	27.643646554201023	27.51766715205059	22.446158008532016
56-57	22.353090939570663	27.799494760978373	27.529609097441686	22.31780520200928
58-59	22.32233022164581	27.7684767296668	27.545293916648777	22.36389913203861
60-61	22.415334771518282	27.770750684679797	27.518064447839937	22.295850095961985
62-63	22.39306594398749	27.81109709474721	27.5041663357473	22.291670625518
64-65	22.32451900256451	27.765683927749617	27.55323401813322	22.35656305155265
66-67	22.37332026363723	27.783855245578838	27.369270342540865	22.47355414824307
68-69	22.337282640214763	27.80177777747841	27.456804210408613	22.40413537189821
70-71	22.310458485428704	27.63311679115823	27.485818745429462	22.57060597798361
72-73	22.359645514843372	27.6721703574922	27.495826434997806	22.472357692666623
74-75	22.204268205702387	27.832093928599992	27.48319246762107	22.480445398076544
76-77	22.33569010122019	27.75395669572015	27.432664703599848	22.477688499459813
78-79	22.270853275450637	27.680602555287415	27.427446645005592	22.621097524256356
80-81	22.25253721143146	27.779673097727336	27.41605125496215	22.551738435879056
82-83	22.315901885384935	27.746855471583054	27.49601105950404	22.44123158352797
84-85	22.3802704554199	27.729469505729114	27.4449564099436	22.445303628907386
86-87	22.224883706172935	27.782976881867654	27.506817750027203	22.48532166193221
88-89	22.282337927790902	27.72771543444859	27.511306642657402	22.4786399951031
90-91	22.315857280159175	27.813931600145715	27.43264163220192	22.43756948749319
92-93	22.31916043382357	27.769135384508992	27.499965823567585	22.411738358099857
94-95	22.1684949353557	27.81123662544972	27.585849116109085	22.434419323085493
96-97	22.155003612583002	27.952337177234487	27.631806386708234	22.260852823474277
98-99	23.318764625653014	29.886165120077944	27.85644755962525	18.93862269464379
100	22.30397214786671	30.930451619655656	21.50433905983252	25.261237172645114
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1091.0
1	871.5
2	940.5
3	1306.5
4	1705.5
5	2574.5
6	3392.5
7	4099.0
8	4594.0
9	4651.5
10	4246.5
11	3704.5
12	3835.5
13	4960.0
14	7763.5
15	11455.0
16	14333.5
17	16097.5
18	16873.0
19	16877.5
20	16438.0
21	16004.5
22	16197.0
23	17799.0
24	21061.0
25	25705.5
26	31700.0
27	38636.5
28	46245.5
29	54650.0
30	62663.0
31	69914.0
32	76903.5
33	83767.5
34	91298.5
35	98225.0
36	105686.5
37	113130.0
38	118777.5
39	124190.0
40	129698.5
41	133811.5
42	136674.0
43	140714.5
44	144078.0
45	146980.5
46	149458.5
47	150565.0
48	151823.5
49	151620.5
50	150283.5
51	148172.5
52	143499.0
53	137682.0
54	130892.5
55	122887.5
56	114722.5
57	104867.5
58	93319.5
59	81076.0
60	68562.0
61	56390.5
62	45257.5
63	35506.5
64	26681.5
65	19675.0
66	14677.0
67	11212.0
68	8628.5
69	6290.5
70	4564.5
71	3369.5
72	2428.0
73	1714.0
74	1192.0
75	809.5
76	539.5
77	355.0
78	233.0
79	159.0
80	104.0
81	68.5
82	52.0
83	37.0
84	26.0
85	21.0
86	17.0
87	14.0
88	8.0
89	3.0
90	2.5
91	2.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	5.6173305883311185E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0013762459941411241
20-21	0.0025700341323003015
22-23	0.004733289661596475
24-25	9.552916484330057E-4
26-27	0.0015459297847124126
28-29	0.0035581493010697794
30-31	0.007746825843749057
32-33	0.01310478660803781
34-35	1.7005183179833212E-4
36-37	7.835055834744712E-4
38-39	0.0015210138102314296
40-41	0.0021693740213411445
42-43	2.9170877604394184E-4
44-45	1.4730011411339841E-5
46-47	0.0032768249829902996
48-49	0.0030721845913075853
50-51	5.630173101954522E-4
52-53	9.98748491313577E-4
54-55	9.158103322653018E-4
56-57	0.0013498809907247478
58-59	6.668764681206056E-4
60-61	3.8548476395563265E-4
62-63	0.001607876842480691
64-65	3.77760298608019E-4
66-67	0.001629252056930722
68-69	0.0012461059190031153
70-71	5.801117226929468E-4
72-73	4.666075745617302E-4
74-75	0.0074381504662582735
76-77	0.0029083377076624057
78-79	0.005644143239727435
80-81	9.11697701464223E-5
82-83	0.004051681697665731
84-85	0.002139573781888095
86-87	5.526201053103362E-4
88-89	0.002866772556463797
90-91	7.494780772731621E-4
92-93	0.0
94-95	5.752793032381445E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	88.0
20-21	314.0
22-23	603.0
24-25	1250.0
26-27	2074.0
28-29	4265.0
30-31	8610.0
32-33	11272.0
34-35	16482.0
36-37	24127.0
38-39	27303.0
40-41	28444.0
42-43	32163.0
44-45	37042.0
46-47	37344.0
48-49	35016.0
50-51	32100.0
52-53	32134.0
54-55	35239.0
56-57	36490.0
58-59	36227.0
60-61	34818.0
62-63	33915.0
64-65	36172.0
66-67	37987.0
68-69	38807.0
70-71	37864.0
72-73	36307.0
74-75	37369.0
76-77	37144.0
78-79	39298.0
80-81	39961.0
82-83	38304.0
84-85	39784.0
86-87	41624.0
88-89	45406.0
90-91	47612.0
92-93	51305.0
94-95	61779.0
96-97	134450.0
98-99	226363.0
100-101	2025554.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.0608364116943
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.04742558232535	81.97820711905082
2	5.9219691858410055	10.548310577905607
3	1.2249156335755507	3.27276032579997
4	0.3951872982531457	1.407828452868114
5	0.16812014064709596	0.7486460171841024
6	0.09029863932057523	0.48252434068370015
7	0.04425931641128334	0.2759240217019106
8	0.031029864368175116	0.22108365394968849
9	0.016429531183466618	0.13169050101463953
>10	0.05887006650485872	0.7852977909616938
>50	8.428767559381672E-4	0.05202119693946413
>100	6.518648135997445E-4	0.09570600194030589
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.617330588331119E-5	0.0	0.0	2.8086652941655596E-5
3	0.0	5.617330588331119E-5	0.0	0.0	2.8086652941655596E-5
4	0.0	8.425995882496679E-5	0.0	0.0	2.8086652941655596E-5
5	0.0	8.425995882496679E-5	0.0	0.0	2.8086652941655596E-5
6	0.0	8.425995882496679E-5	0.0	0.0	2.8086652941655596E-5
7	0.0	1.1234661176662238E-4	0.0	0.0	2.8086652941655596E-5
8	0.0	1.1234661176662238E-4	0.0	0.0	2.8086652941655596E-5
9	0.0	1.9660657059158916E-4	0.0	0.0	2.8086652941655596E-5
10-11	0.0	1.9660657059158916E-4	0.0	4.2129979412483396E-5	2.8086652941655596E-5
12-13	0.0	1.9660657059158916E-4	0.0	5.617330588331119E-5	2.8086652941655596E-5
14-15	0.0	2.3873655000407259E-4	0.0	7.021663235413899E-5	8.425995882496679E-5
16-17	0.0	2.527798764749004E-4	0.0	8.425995882496679E-5	1.1234661176662238E-4
18-19	0.0	2.527798764749004E-4	0.0	8.425995882496679E-5	1.1234661176662238E-4
20-21	0.0	2.527798764749004E-4	0.0	9.830328529579458E-5	1.1234661176662238E-4
22-23	0.0	2.9490985588738374E-4	0.0	1.1234661176662238E-4	1.263899382374502E-4
24-25	0.0	3.3703983529986717E-4	0.0	1.4043326470827798E-4	1.4043326470827798E-4
26-27	0.0	3.6512648824152275E-4	0.0	2.1064989706241698E-4	1.4043326470827798E-4
28-29	0.0	3.6512648824152275E-4	0.0	4.4938644706648954E-4	1.4043326470827798E-4
30-31	0.0	4.3534312059566175E-4	0.0	0.0013341160147286408	1.4043326470827798E-4
32-33	0.0	4.4938644706648954E-4	0.0	0.0032720950677028768	1.6851991764993358E-4
34-35	0.0	5.617330588331119E-4	0.0	0.006038630382455954	2.2469322353324477E-4
36-37	0.0	6.740796705997343E-4	0.0	0.009605635306046214	2.2469322353324477E-4
38-39	0.0	7.302529764830455E-4	0.0	0.015391485812027266	2.2469322353324477E-4
40-41	0.0	8.145129353080122E-4	0.0	0.02194971927390385	2.2469322353324477E-4
42-43	0.0	8.285562617788401E-4	0.0	0.02805856628871394	2.2469322353324477E-4
44-45	0.0	9.68989526487118E-4	0.0	0.034209543282936516	2.2469322353324477E-4
46-47	0.0	0.0010953794647245681	0.0	0.04175080959777104	3.089531823582116E-4
48-49	0.0	0.001109422791195396	0.0	0.048758429506714114	3.3703983529986717E-4
50-51	0.0	0.0012919860353161575	0.0	0.05621543586272368	3.3703983529986717E-4
52-53	0.0	0.0012919860353161575	0.0	0.06326518575107923	3.6512648824152275E-4
54-55	0.0	0.0013060293617869851	0.0	0.0706800621276763	4.0725646765400617E-4
56-57	0.0	0.0013481593411994687	0.0	0.07855836827781071	4.2129979412483396E-4
58-59	0.0	0.0013762459941411244	0.0	0.0862400678573535	4.2129979412483396E-4
60-61	0.0	0.0014324193000244353	0.0	0.09430093725160865	4.2129979412483396E-4
62-63	0.0	0.0014324193000244353	0.0	0.10153325038408498	4.2129979412483396E-4
64-65	0.0	0.0015166792588494021	0.0	0.1084144803547906	4.2129979412483396E-4
66-67	0.0	0.0015447659117910578	0.0	0.11560466350785442	4.2129979412483396E-4
68-69	0.0	0.0015868958912035411	0.0	0.12223311360208516	4.2129979412483396E-4
70-71	0.0	0.001600939217674369	0.0	0.1277661842315913	4.2129979412483396E-4
72-73	0.0	0.001600939217674369	0.0	0.13321499490227248	4.4938644706648954E-4
74-75	0.0	0.0016430691970868525	0.0	0.13709095300822097	4.4938644706648954E-4
76-77	0.0	0.001741372482382647	0.0	0.14075626121710702	4.4938644706648954E-4
78-79	0.0	0.001741372482382647	0.0	0.14321384334950188	4.4938644706648954E-4
80-81	0.0	0.001741372482382647	0.0	0.144182832875989	4.6342977353731733E-4
82-83	0.0	0.001741372482382647	0.0	0.1449833024848262	4.774731000081451E-4
84-85	0.0	0.001741372482382647	0.0	0.1452501256877719	4.774731000081451E-4
86-87	0.0	0.0017694591353243025	0.0	0.14537651562600937	4.774731000081451E-4
88	0.0	0.001881805747090925	0.0	0.14537651562600937	5.055597529498008E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3985	0.0	47.980507	1
GTATCAA	7265	0.0	33.211166	1
TCAACGC	8390	0.0	28.313993	4
ATCAACG	8515	0.0	27.898344	3
CAACGCA	8640	0.0	27.542625	5
AACGCAG	8765	0.0	27.197046	6
TATCAAC	9105	0.0	26.40872	2
ACGCAGA	10300	0.0	23.063534	7
CGCAGAG	10715	0.0	22.131641	8
GTGGTAT	2195	0.0	20.928576	1
GTACATG	9615	0.0	20.23018	1
TACATGG	9760	0.0	19.42079	2
TGGTATC	2180	0.0	19.36399	2
GCAGAGT	12340	0.0	18.948921	9
ACATGGG	9760	0.0	18.69993	3
GAGTACT	7140	0.0	18.287674	12-13
CAGAGTA	11925	0.0	17.664877	10-11
CATGGGG	7330	0.0	17.502855	4
AGAGTAC	11040	0.0	16.887949	10-11
GTACTTT	8325	0.0	15.760461	14-15
>>END_MODULE