FastQCFastQC Report
Fri 27 May 2016
SRR2138713_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138713_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3868836
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39100.049.0595441
GTATCAA72800.033.0366361
TCAACGC85300.027.561244
ATCAACG86000.027.3369063
CAACGCA87900.026.8406815
TATCAAC91100.026.0805722
AACGCAG90950.025.8948336
ACGCAGA107450.021.9184257
CGCAGAG110350.021.2669948
GTACATG107100.020.7079491
TACATGG107850.019.7537332
ACATGGG110200.018.6528283
GCAGAGT129900.018.0663039
GAGTACT73000.017.75579612-13
TGGTATC21600.017.3375662
GTGGTAT24650.016.5427821
AGAGTAC117550.016.5130910-11
GTACTTT79700.016.34262714-15
CATGGGG81000.016.335844
GTATACG2551.0721596E-616.3177091
Produced by FastQC (version 0.11.5)