FastQCFastQC Report
Fri 27 May 2016
SRR2138712_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138712_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5455327
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59450.045.961111
GTATCAA105250.034.407921
TCAACGC124250.028.4570754
ATCAACG126600.027.9610563
CAACGCA128200.027.612095
TATCAAC131600.027.20862
AACGCAG132650.026.8395086
CGTACGG1358.752615E-526.21600584-85
ACGCAGA156200.022.7929637
CGCAGAG161550.021.9876488
GTACATG158300.021.0478951
TACATGG157650.020.4362262
GTGGTAT32050.019.3411871
ACATGGG166300.019.2015843
GCAGAGT190350.018.4895119
GAGTACT104700.017.8203412-13
CATGGGG123650.017.4803164
TGGTATC32500.017.1911432
AGAGTAC166550.016.87105210-11
GAGTACA115800.016.7635481
Produced by FastQC (version 0.11.5)