##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138712_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5455327
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.773320462733032	31.0	31.0	34.0	30.0	34.0
2	32.042299205895446	33.0	31.0	34.0	30.0	34.0
3	31.949996398016104	31.0	31.0	34.0	30.0	34.0
4	35.80068014254691	37.0	35.0	37.0	35.0	37.0
5	35.5817700753777	37.0	35.0	37.0	33.0	37.0
6	35.66487618432406	37.0	35.0	37.0	33.0	37.0
7	35.584974832856034	37.0	35.0	37.0	33.0	37.0
8	35.57077880024424	37.0	35.0	37.0	33.0	37.0
9	37.29500559728134	39.0	37.0	39.0	34.0	39.0
10-11	37.17009282853255	39.0	37.0	39.0	33.0	39.0
12-13	37.12959608104153	39.0	37.0	39.0	33.0	39.0
14-15	38.320072014014926	40.0	38.0	41.0	33.0	41.0
16-17	38.27376855686194	40.0	38.0	41.0	33.0	41.0
18-19	38.27044666616685	40.0	38.0	41.0	33.5	41.0
20-21	38.270064448715175	40.0	38.0	41.0	33.0	41.0
22-23	38.203450318739314	40.0	38.0	41.0	33.0	41.0
24-25	38.12228734393454	40.0	37.0	41.0	33.0	41.0
26-27	37.952475030414924	40.0	37.0	41.0	33.0	41.0
28-29	37.80172451691286	40.0	37.0	41.0	32.5	41.0
30-31	37.644733133977454	40.0	37.0	41.0	32.0	41.0
32-33	37.48298024508135	39.0	36.0	41.0	32.0	41.0
34-35	37.35590500616978	39.0	36.0	41.0	31.0	41.0
36-37	37.4490105045855	39.0	36.0	41.0	31.5	41.0
38-39	37.417726189584776	39.0	36.0	41.0	31.0	41.0
40-41	37.38259090582215	39.0	36.0	41.0	31.0	41.0
42-43	37.46456696299444	39.5	36.0	41.0	31.5	41.0
44-45	37.501943609349155	40.0	36.0	41.0	32.0	41.0
46-47	37.38389411257202	39.5	36.0	41.0	31.0	41.0
48-49	37.32278443816597	39.0	36.0	41.0	31.0	41.0
50-51	37.22713709486548	39.0	36.0	41.0	31.0	41.0
52-53	37.07783686360122	39.0	35.5	41.0	31.0	41.0
54-55	36.902629531927985	39.0	35.0	40.5	31.0	41.0
56-57	36.76783514112072	39.0	35.0	40.0	31.0	41.0
58-59	36.50915353978215	38.0	35.0	40.0	30.0	41.0
60-61	36.3486190272256	38.0	35.0	40.0	30.0	41.0
62-63	36.088826141785056	38.0	34.0	40.0	29.5	41.0
64-65	35.78234838734764	37.0	34.0	40.0	29.0	41.0
66-67	35.453403466289174	37.0	34.0	39.5	29.0	41.0
68-69	35.2224191959079	36.0	34.0	39.0	29.0	41.0
70-71	34.89824680650078	36.0	33.5	39.0	29.0	40.0
72-73	34.60469595560479	35.0	33.0	38.5	28.0	40.0
74-75	34.319813430874405	35.0	33.0	37.5	28.0	39.5
76-77	32.963138975224425	34.0	31.5	36.0	26.5	39.0
78-79	33.713063266409065	35.0	32.5	36.0	28.0	39.0
80-81	33.74088263844992	35.0	33.0	36.0	29.0	38.5
82-83	33.50362398174392	35.0	33.0	36.0	29.0	37.0
84-85	33.30051823221174	35.0	33.0	35.5	29.0	37.0
86-87	33.12848525471482	35.0	33.0	35.0	28.5	36.5
88-89	33.01111744529953	35.0	32.0	35.0	28.5	36.0
90-91	32.875370450685644	35.0	32.0	35.0	28.0	36.0
92-93	32.86745518389083	34.0	32.0	35.0	29.0	36.0
94-95	32.80583576845329	34.0	32.0	35.0	29.0	35.0
96-97	32.905421374614576	34.0	32.0	35.0	29.0	35.0
98-99	33.031402331179706	34.0	32.0	35.0	29.0	35.0
100	31.965622972221453	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	7.0
20	45.0
21	301.0
22	1294.0
23	4291.0
24	11143.0
25	23329.0
26	40826.0
27	63807.0
28	90209.0
29	117563.0
30	147627.0
31	180167.0
32	220028.0
33	270909.0
34	349769.0
35	466288.0
36	679191.0
37	1045412.0
38	1150857.0
39	572529.0
40	19732.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.006941105455276	20.036177483036305	13.579992546734596	24.376888864773825
2	15.695594416246724	21.085775426477642	39.555685662839274	23.662944494436356
3	18.7907790458991	25.233671242388617	30.159583847969156	25.815965863743134
4	13.214093307330616	17.243750924555027	37.534688571372534	32.00746719674183
5	13.574713303162211	37.53292882351507	35.26661188229413	13.625745991028584
6	31.538256093539395	36.92062822265283	17.915351362072336	13.625764321735435
7	27.628297992036043	32.067940198635206	22.346836404123895	17.956925405204856
8	25.393216575285038	36.096919579706224	20.62800268434871	17.88186116066003
9	25.88972210098496	16.18352117114153	20.876988675472617	37.04976805240089
10-11	25.007758471673654	26.292667698929872	28.282310116332166	20.41726371306431
12-13	25.33591478567646	23.759180705391262	28.623902838454963	22.281001670477316
14-15	22.45745305460149	25.286916806270277	26.02786230779566	26.227767831332567
16-17	22.301046298416207	28.395658042130194	26.787019366575095	22.5162762928785
18-19	22.292073519411932	27.6425627142493	28.38758041695511	21.677783349383652
20-21	22.977833711127875	26.81678232709827	28.29953958868211	21.90584437309174
22-23	22.778220915614956	26.71005495897214	28.109289799830755	22.402434325582156
24-25	22.55081896263205	26.847459212332442	28.188911109338367	22.412810715697145
26-27	22.49861476023426	27.02356925831878	28.149044459774842	22.328771521672124
28-29	22.466124561724037	27.062194561017105	28.15985148882999	22.311829388428873
30-31	22.68830819589872	26.941426754657193	27.99378513885215	22.376479910591936
32-33	22.40612355841429	27.148641546726903	27.993337412581443	22.451897482277364
34-35	22.59898432779785	27.14103166779836	27.99657194888077	22.26341205552302
36-37	22.619482637723607	27.103884083659135	27.887515324259372	22.389117954357886
38-39	22.47468790335862	27.219977341691298	27.99494260687421	22.310392148075874
40-41	22.703724480784285	27.056851180520745	27.843789164748618	22.395635173946353
42-43	22.524431091556178	27.210486910173287	27.64880725306392	22.616274745206617
44-45	22.575675614725995	27.27516102703059	27.541083440016855	22.608079918226558
46-47	22.720856472451626	27.240154154063895	27.33221729763849	22.706772075845997
48-49	22.592070665884954	27.37347566707905	27.295509672674783	22.738943994361208
50-51	22.59078209487261	27.46185466033487	27.227764107051822	22.719599137740705
52-53	22.81989451568823	27.31918895366826	27.100540812527452	22.76037571811605
54-55	22.747720580482188	27.24586877649647	27.198614644657543	22.8077959983638
56-57	22.684393938796184	27.39826790118868	27.159231703774516	22.758106456240615
58-59	22.6459848585126	27.42224319803757	27.185192887376235	22.74657905607359
60-61	22.734101007804274	27.38449488327018	27.24676993589371	22.63463417303184
62-63	22.789626213476122	27.50449986284388	27.085360237912536	22.62051368576746
64-65	22.67949394038383	27.42356016288405	27.13620625435936	22.76073964237276
66-67	22.787438976497405	27.407026030025655	26.999018141556284	22.806516851920648
68-69	22.67490771855521	27.403122696046093	27.1200859163956	22.801883669003097
70-71	22.67489616504648	27.21324922412231	27.182682450898216	22.929172159932996
72-73	22.762555480344034	27.29473295612827	27.034667312719947	22.908044250807748
74-75	22.57044159043935	27.391305008326704	27.119073074571986	22.919180326661962
76-77	22.73961583038564	27.23716736759636	27.08649820566186	22.93671859635613
78-79	22.639960301348854	27.243403858588593	27.105360821641778	23.011275018420775
80-81	22.73641406196934	27.317913075602558	27.05055319123983	22.89511967118827
82-83	22.70767103050184	27.30212079212246	27.161920259566447	22.828287917809252
84-85	22.76906938348064	27.264120191387754	27.100529495359638	22.866280929771968
86-87	22.64258445497849	27.24151541597305	27.248589373429343	22.86731075561912
88-89	22.687389940713295	27.162432992429235	27.21773906830855	22.932437998548917
90-91	22.689902396043195	27.203734403002684	27.275384008750198	22.830979192203927
92-93	22.765594915131917	27.115490964660644	27.269340652156863	22.849573468050572
94-95	22.603172428808847	27.20069116193108	27.340432611015657	22.85570379824442
96-97	22.58601620784653	27.26965869297306	27.47207004823285	22.672255050947566
98-99	23.7271068712497	29.269587126807384	27.598288128493653	19.405017873449264
100	30.588642939545085	40.871012026048334	28.540345034406585	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	896.0
1	849.0
2	1229.5
3	1767.5
4	2420.0
5	3887.0
6	5261.5
7	6418.5
8	7264.5
9	7276.0
10	6569.5
11	5721.0
12	5952.0
13	7774.5
14	12167.5
15	18107.5
16	23003.5
17	26140.5
18	27353.5
19	26767.5
20	25328.5
21	23983.5
22	23431.5
23	24788.0
24	28691.5
25	35198.5
26	44232.0
27	54051.5
28	64122.0
29	76222.0
30	88124.5
31	98835.5
32	110679.5
33	122270.0
34	133257.5
35	144881.5
36	157689.5
37	169826.5
38	179300.0
39	185857.0
40	193740.5
41	202950.5
42	208991.5
43	214840.0
44	221547.0
45	227528.0
46	230709.5
47	233417.0
48	235910.0
49	236832.0
50	237578.5
51	235734.0
52	229781.5
53	221594.5
54	212202.0
55	200409.0
56	187046.5
57	172136.0
58	154948.5
59	135917.0
60	117239.5
61	98581.5
62	80111.0
63	63338.0
64	49170.0
65	38954.5
66	30604.0
67	23198.0
68	17556.5
69	13258.5
70	9563.0
71	6736.0
72	4764.0
73	3277.0
74	2206.0
75	1500.0
76	1032.0
77	683.5
78	469.5
79	305.5
80	199.0
81	137.0
82	95.5
83	69.0
84	52.5
85	41.5
86	31.5
87	25.5
88	18.0
89	13.0
90	8.5
91	3.5
92	1.5
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	2.3829918903119833E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0016039368492484502
20-21	0.0032355153630610287
22-23	0.005151765795226844
24-25	0.0011553277664873526
26-27	0.0017613334468414886
28-29	0.004296503545808898
30-31	0.008248891020746467
32-33	0.014600874982567496
34-35	2.407853752149241E-4
36-37	0.0010900859108818432
38-39	0.0021889337281054957
40-41	0.0027588477004198567
42-43	3.637738318528217E-4
44-45	1.935740575145304E-5
46-47	0.003920346401808063
48-49	0.003839613300341101
50-51	8.730901278896398E-4
52-53	0.0012072015353980401
54-55	0.0011795444025130654
56-57	0.0016195727442518146
58-59	7.990277934458483E-4
60-61	4.364428967162143E-4
62-63	0.0018208140159152075
64-65	5.56250201777034E-4
66-67	0.00181269690281944
68-69	0.0017035939331433527
70-71	4.5469147251151333E-4
72-73	8.183172124842474E-4
74-75	0.008471472870137345
76-77	0.0034732125224365973
78-79	0.006603975809961394
80-81	9.773255583827412E-5
82-83	0.004914285430632876
84-85	0.002760469521832478
86-87	8.714219478894801E-4
88-89	0.003822614010441346
90-91	0.0010246959713745192
92-93	8.154570426064072E-5
94-95	6.821363117168048E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	167.0
20-21	518.0
22-23	1045.0
24-25	2434.0
26-27	3473.0
28-29	7074.0
30-31	15196.0
32-33	20035.0
34-35	28999.0
36-37	41935.0
38-39	48566.0
40-41	50024.0
42-43	54401.0
44-45	63708.0
46-47	63296.0
48-49	58596.0
50-51	54143.0
52-53	52694.0
54-55	57815.0
56-57	60593.0
58-59	59157.0
60-61	56793.0
62-63	55898.0
64-65	59499.0
66-67	62543.0
68-69	62550.0
70-71	61289.0
72-73	58960.0
74-75	61050.0
76-77	60504.0
78-79	63465.0
80-81	64151.0
82-83	62092.0
84-85	64317.0
86-87	67815.0
88-89	73949.0
90-91	77528.0
92-93	84293.0
94-95	97446.0
96-97	209787.0
98-99	1108386.0
100-101	2199143.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.02936824996783
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.63166446312144	80.66277534381608
2	6.064403162141281	10.676911583528085
3	1.3650931120622427	3.6050485277166526
4	0.43520969835561346	1.532449392100149
5	0.1943965334015483	0.8556302012661033
6	0.10228100604666433	0.54022394077554
7	0.05759825179322026	0.35492364023618295
8	0.03652189654422634	0.2571999584063143
9	0.025645873703026015	0.2031831054266259
>10	0.08538428988004822	1.1780014587108003
>50	0.0015532331863777303	0.08888670312909647
>100	2.180587528326149E-4	0.031055079712057048
>500	3.042101142474859E-5	0.013711065176297844
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8330706848553717E-5
2	0.0	3.6661413697107435E-5	0.0	0.0	1.8330706848553717E-5
3	0.0	3.6661413697107435E-5	0.0	0.0	1.8330706848553717E-5
4	0.0	1.099842410913223E-4	0.0	3.6661413697107435E-5	1.8330706848553717E-5
5	0.0	1.099842410913223E-4	0.0	3.6661413697107435E-5	1.8330706848553717E-5
6	9.165353424276858E-5	1.099842410913223E-4	0.0	3.6661413697107435E-5	3.6661413697107435E-5
7	9.165353424276858E-5	1.099842410913223E-4	0.0	3.6661413697107435E-5	3.6661413697107435E-5
8	9.165353424276858E-5	1.4664565478842974E-4	0.0	3.6661413697107435E-5	5.499212054566115E-5
9	9.165353424276858E-5	3.666141369710743E-4	0.0	3.6661413697107435E-5	7.332282739421487E-5
10-11	9.165353424276858E-5	3.666141369710743E-4	0.0	3.6661413697107435E-5	7.332282739421487E-5
12-13	9.165353424276858E-5	3.666141369710743E-4	0.0	3.6661413697107435E-5	7.332282739421487E-5
14-15	9.165353424276858E-5	4.399369643652892E-4	0.0	3.6661413697107435E-5	9.165353424276858E-5
16-17	1.2831494793987601E-4	4.582676712138429E-4	0.0	3.6661413697107435E-5	9.165353424276858E-5
18-19	1.2831494793987601E-4	5.22425145183781E-4	0.0	3.6661413697107435E-5	9.165353424276858E-5
20-21	1.2831494793987601E-4	5.86582619153719E-4	0.0	6.415747396993801E-5	9.165353424276858E-5
22-23	1.2831494793987601E-4	6.507400931236569E-4	0.0	1.0998424109132229E-4	1.1914959451559917E-4
24-25	1.2831494793987601E-4	6.690707999722106E-4	0.0	1.4664565478842974E-4	1.4664565478842974E-4
26-27	1.2831494793987601E-4	6.874015068207644E-4	0.0	1.92472421909814E-4	1.4664565478842974E-4
28-29	1.2831494793987601E-4	6.965668602450412E-4	0.0	5.682519123051652E-4	1.4664565478842974E-4
30-31	1.2831494793987601E-4	7.515589807907024E-4	0.0	0.0016955903834912187	1.4664565478842974E-4
32-33	1.2831494793987601E-4	7.515589807907024E-4	0.0	0.0042527239888644625	1.4664565478842974E-4
34-35	1.2831494793987601E-4	9.257006958519626E-4	0.0	0.007488093747634193	1.6497636163698343E-4
36-37	1.2831494793987601E-4	0.001099842410913223	0.0	0.012318235002228096	1.6497636163698343E-4
38-39	1.2831494793987601E-4	0.0011181731177617768	0.0	0.020182108240257644	1.741417150612603E-4
40-41	1.2831494793987601E-4	0.0011914959451559915	0.0	0.027963493297468694	1.8330706848553716E-4
42-43	1.2831494793987601E-4	0.0012189920054288222	0.0	0.03500248472731332	1.8330706848553716E-4
44-45	1.2831494793987601E-4	0.0013289762465201445	0.0	0.04368207442010351	1.92472421909814E-4
46-47	1.2831494793987601E-4	0.0014114644273386361	0.0	0.05293908137862313	2.0163777533409088E-4
48-49	1.2831494793987601E-4	0.0014114644273386361	0.0	0.0619027970275659	2.382991890311983E-4
50-51	1.3748030136415286E-4	0.00160393684924845	0.0	0.07030742611762777	2.382991890311983E-4
52-53	1.4664565478842974E-4	0.0016314329095212807	0.0	0.07858374025974979	2.4746454245547515E-4
54-55	1.4664565478842974E-4	0.0016497636163698343	0.0	0.0864201174375065	2.657952493040289E-4
56-57	1.4664565478842974E-4	0.0016680943232183882	0.0	0.09487057329468976	2.749606027283057E-4
58-59	1.4664565478842974E-4	0.001677259676642665	0.0	0.10341268268611578	2.749606027283057E-4
60-61	1.4664565478842974E-4	0.0017047557369154957	0.0	0.11296298095421228	2.749606027283057E-4
62-63	1.4664565478842974E-4	0.001741417150612603	0.0	0.12090934237306031	2.749606027283057E-4
64-65	1.558110082127066E-4	0.00207136987388657	0.0	0.12892902661930256	2.749606027283057E-4
66-67	1.741417150612603E-4	0.0020897005807351235	0.0	0.13776442732030547	2.749606027283057E-4
68-69	1.8330706848553716E-4	0.0021263619944322312	0.0	0.14597658398845753	2.749606027283057E-4
70-71	2.1080312875836776E-4	0.0021721887615536156	0.0	0.15234650461832994	2.749606027283057E-4
72-73	2.5662989587975203E-4	0.002181354114977892	0.0	0.15789154344001743	3.8494484381962807E-4
74-75	2.5662989587975203E-4	0.002208850175250723	0.0	0.1619792910672449	3.8494484381962807E-4
76-77	2.749606027283057E-4	0.0022913383560692144	0.0	0.16655280242595905	3.8494484381962807E-4
78-79	2.749606027283057E-4	0.002327999769766322	0.0	0.1692840777463936	3.8494484381962807E-4
80-81	2.749606027283057E-4	0.002327999769766322	0.0	0.17034725874360968	3.8494484381962807E-4
82-83	2.749606027283057E-4	0.002327999769766322	0.0	0.1709338413627634	4.0327555066818177E-4
84-85	2.749606027283057E-4	0.0023646611834634295	0.0	0.1712088019654917	4.0327555066818177E-4
86-87	2.749606027283057E-4	0.002382991890311983	0.0	0.17126379408603737	4.0327555066818177E-4
88	2.932913095768595E-4	0.0024746454245547517	0.0	0.17126379408603737	4.2160625751673546E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5945	0.0	45.96111	1
GTATCAA	10525	0.0	34.40792	1
TCAACGC	12425	0.0	28.457075	4
ATCAACG	12660	0.0	27.961056	3
CAACGCA	12820	0.0	27.61209	5
TATCAAC	13160	0.0	27.2086	2
AACGCAG	13265	0.0	26.839508	6
CGTACGG	135	8.752615E-5	26.216005	84-85
ACGCAGA	15620	0.0	22.792963	7
CGCAGAG	16155	0.0	21.987648	8
GTACATG	15830	0.0	21.047895	1
TACATGG	15765	0.0	20.436226	2
GTGGTAT	3205	0.0	19.341187	1
ACATGGG	16630	0.0	19.201584	3
GCAGAGT	19035	0.0	18.489511	9
GAGTACT	10470	0.0	17.82034	12-13
CATGGGG	12365	0.0	17.480316	4
TGGTATC	3250	0.0	17.191143	2
AGAGTAC	16655	0.0	16.871052	10-11
GAGTACA	11580	0.0	16.763548	1
>>END_MODULE