FastQCFastQC Report
Fri 27 May 2016
SRR2138711_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138711_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3766102
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38100.045.6316951
GTATCAA67100.033.0937041
TCAACGC76650.027.6805154
ATCAACG77850.027.359683
TACGCTA1807.844495E-426.99093694
CAACGCA79600.026.6546675
TATCAAC81350.026.3851342
AACGCAG81600.026.2033186
ACGCAGA96800.022.003637
CGCAGAG99250.021.5019768
GTACATG97600.021.2745231
TACATGG101100.020.4972672
GTGGTAT20700.020.3006041
ACATGGG100050.020.1358933
GCAGAGT119650.017.8703789
TGGTATC20700.017.5142462
CATGGGG75150.017.3235634
GAGTACT67700.016.9478912-13
GGACCGT4750.016.4793136
AGAGTAC105000.016.42045810-11
Produced by FastQC (version 0.11.5)