##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138711_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3766102
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17873413943648	33.0	31.0	34.0	30.0	34.0
2	32.40479811752311	34.0	31.0	34.0	30.0	34.0
3	32.34295645736626	34.0	31.0	34.0	30.0	34.0
4	35.31815176540625	35.0	34.0	37.0	34.0	37.0
5	35.1502604018691	35.0	34.0	37.0	33.0	37.0
6	36.04731629679706	37.0	35.0	37.0	35.0	38.0
7	35.984224537731585	37.0	35.0	37.0	33.0	38.0
8	35.94190837104252	37.0	35.0	37.0	33.0	38.0
9	37.26903971267905	38.0	37.0	39.0	34.0	39.0
10-11	37.18009602501472	38.0	37.0	39.0	34.0	39.0
12-13	37.12516070993297	38.0	37.0	39.0	34.0	39.0
14-15	38.06471877288507	38.5	38.0	41.0	34.0	41.0
16-17	38.004228775535026	38.0	38.0	41.0	34.0	41.0
18-19	38.02418256329754	38.5	38.0	41.0	34.0	41.0
20-21	38.017814504443066	39.0	38.0	41.0	34.0	41.0
22-23	37.96285945887957	39.0	38.0	41.0	34.0	41.0
24-25	37.89532494927499	38.5	38.0	41.0	34.0	41.0
26-27	37.74607451036153	38.0	37.0	41.0	33.5	41.0
28-29	37.607990261061644	38.0	37.0	40.0	33.0	41.0
30-31	37.50838083415032	38.0	37.0	40.0	33.0	41.0
32-33	37.36563205002197	38.0	37.0	40.0	33.0	41.0
34-35	37.266098861711384	38.0	37.0	40.0	32.5	41.0
36-37	37.323417611980645	38.0	37.0	40.0	33.0	41.0
38-39	37.30951656156446	38.0	37.0	40.0	33.0	41.0
40-41	37.28167703795369	38.0	37.0	40.0	33.0	41.0
42-43	37.347605413300556	38.0	37.0	40.0	33.0	41.0
44-45	37.34839312734775	38.0	37.0	40.0	33.0	41.0
46-47	37.246086346541574	38.0	37.0	40.0	32.0	41.0
48-49	37.196258994303946	38.0	37.0	40.0	32.0	41.0
50-51	37.133123206942656	38.0	37.0	40.0	32.0	41.0
52-53	37.02144659773579	38.0	36.0	40.0	31.5	41.0
54-55	36.883944700661786	38.0	36.0	40.0	31.0	41.0
56-57	36.77983676334236	38.0	36.0	40.0	31.0	41.0
58-59	36.59600977370151	38.0	35.5	40.0	31.0	41.0
60-61	36.467563081265126	38.0	35.0	40.0	31.0	41.0
62-63	36.29187301652019	38.0	35.0	39.5	31.0	41.0
64-65	36.05142202226964	38.0	35.0	39.0	30.0	41.0
66-67	35.79515659092695	38.0	35.0	39.0	30.0	41.0
68-69	35.60135539120716	37.0	34.0	39.0	30.0	40.0
70-71	35.36814450459893	37.0	34.0	38.0	29.0	40.0
72-73	35.12524309967009	36.5	34.0	38.0	29.0	40.0
74-75	34.91415328783974	36.0	34.0	38.0	29.0	39.0
76-77	33.87852316232326	35.0	32.5	38.0	27.5	38.5
78-79	34.43781899353125	35.0	33.5	38.0	29.0	39.0
80-81	34.4358701337092	35.0	34.0	38.0	29.0	38.0
82-83	34.255618470921725	35.0	34.0	37.5	29.0	38.0
84-85	34.08429667345712	35.0	34.0	37.0	29.0	38.0
86-87	33.94793559182883	35.0	33.0	37.0	29.0	38.0
88-89	33.86005807920212	35.0	33.0	37.0	29.0	38.0
90-91	33.73574795870592	35.0	33.0	36.0	29.0	38.0
92-93	33.74681752704646	35.0	33.0	36.0	29.0	38.0
94-95	33.67371858883957	35.0	33.0	36.0	29.0	38.0
96-97	33.75899746615369	35.0	33.0	36.0	29.0	38.0
98-99	33.83512444110494	35.0	33.0	36.0	29.0	38.0
100	32.94845449582781	34.0	31.0	35.0	27.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	3.0
17	13.0
18	32.0
19	42.0
20	143.0
21	369.0
22	1138.0
23	3075.0
24	7274.0
25	14274.0
26	24357.0
27	37491.0
28	51990.0
29	67621.0
30	86241.0
31	107742.0
32	133804.0
33	169488.0
34	222890.0
35	303489.0
36	461328.0
37	1118229.0
38	628120.0
39	316106.0
40	10842.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.859939003245266	20.072345358675893	13.61694399142668	24.45077164665216
2	15.44111126039603	21.06007750188391	39.94655481981104	23.55225641790902
3	18.750912749389688	25.21968384219831	30.15615117413426	25.873252234277743
4	13.036609204955148	17.27109356039746	37.86201223440045	31.83028500024694
5	13.48927883525194	37.53414007374203	35.42639046950932	13.55019062149671
6	31.315402503702767	37.1211932125046	18.03055254477972	13.53285173901291
7	27.313492836890767	32.15345734130409	22.5541156346801	17.97893418712504
8	25.058827402975275	36.472352580997544	20.743782297983433	17.725037718043748
9	25.567602789303105	16.440420360361987	21.023673814463866	36.968303035871045
10-11	24.70108350756299	26.49662701647486	28.50901276704667	20.293276708915478
12-13	25.090318849569133	23.950028437891486	28.80470311213026	22.154949600409125
14-15	22.216445545022413	25.523923674929677	26.143078440254676	26.11655233979324
16-17	22.068839346358647	28.595733732118777	26.991156904406733	22.344270017115843
18-19	22.122568858204257	27.862302576874555	28.497370391214467	21.517758173706728
20-21	22.73253166254413	27.106417216670188	28.43599844392487	21.725052676860816
22-23	22.518644161420383	27.02196401986232	28.256693228721012	22.202698589996288
24-25	22.307163230475812	27.081764843453364	28.345038600621713	22.266033325449104
26-27	22.224939762061304	27.269422907461482	28.301262894346436	22.20437443613078
28-29	22.25176805274912	27.337046687227478	28.27124322024886	22.13994203977454
30-31	22.479971817957427	27.14926018144187	28.15267296715317	22.21809503344754
32-33	22.24577439301726	27.339971877123887	28.165448912044123	22.248804817814733
34-35	22.426627816369642	27.363802448704856	28.109327822693448	22.100241912232054
36-37	22.392555879672717	27.273758527385983	28.096291897292524	22.237393695648777
38-39	22.179903225048804	27.435091801254703	28.18817203790137	22.196832935795126
40-41	22.49726693820693	27.25216475194915	27.984636263903845	22.26593204594008
42-43	22.3547075951601	27.378018619544363	27.869274879523115	22.397998905772422
44-45	22.422585296415697	27.423225485030045	27.743794005993504	22.41039521256075
46-47	22.50021090385634	27.417148641934347	27.587719780863136	22.494920673346183
48-49	22.349116761031482	27.579512194425998	27.57356282399637	22.49780822054616
50-51	22.383364861345484	27.64781225205759	27.51433613658171	22.454486750015214
52-53	22.575949755245617	27.533342258301495	27.389576462673848	22.501131523779044
54-55	22.511062997150404	27.44126708797539	27.45482397900492	22.59284593586928
56-57	22.454935223571436	27.587975103077095	27.479061487728718	22.478028185622748
58-59	22.454587062943702	27.64930519042444	27.42000703261367	22.476100714018195
60-61	22.52633087448918	27.539720229932545	27.47382920320186	22.460119692376416
62-63	22.489672664961326	27.651751838009652	27.442429386441873	22.416146110587146
64-65	22.470901009455574	27.592451266639916	27.406278879808205	22.530368844096305
66-67	22.53265558757941	27.572210064954145	27.287834257292726	22.60730009017372
68-69	22.43812946047891	27.661211022424347	27.36525416567438	22.535405351422362
70-71	22.445773538946558	27.46253407651627	27.400065193382712	22.69162719115446
72-73	22.490811547517424	27.492825679958628	27.34644786551247	22.66991490701148
74-75	22.331759690219172	27.645159840982725	27.353949888591394	22.66913058020671
76-77	22.54007605783525	27.537391504941468	27.259914633916022	22.66261780330726
78-79	22.419350867750538	27.599524424696813	27.22962682315834	22.751497884394308
80-81	22.481155123055807	27.578767613902627	27.298960518287448	22.641116744754118
82-83	22.45607774176797	27.559680582032065	27.35511272700653	22.62912894919344
84-85	22.527312844534574	27.55317971494673	27.313076604495727	22.606430836022966
86-87	22.429679469885745	27.564731015717136	27.392461638447017	22.613127875950102
88-89	22.429832893683237	27.56649389372135	27.32851758898058	22.675155623614835
90-91	22.45769015308923	27.56320012890147	27.394841318167423	22.58426839984187
92-93	22.43159915648771	27.56862028951989	27.421648711212683	22.57813184277972
94-95	22.32066014598483	27.61626287399604	27.418207390409105	22.644869589610025
96-97	22.42518318542221	27.66563369802906	27.47671215652858	22.432470960020147
98-99	23.471975082623075	29.68692432382496	27.658700926743325	19.182399666808642
100	30.048568977618338	41.033393121734385	28.918037900647274	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	671.0
1	579.5
2	685.0
3	948.0
4	1284.5
5	1998.5
6	2660.5
7	3217.5
8	3665.5
9	3711.0
10	3401.0
11	3128.0
12	3385.0
13	4389.5
14	6595.5
15	9477.0
16	11980.5
17	13722.0
18	14479.0
19	14517.0
20	14431.0
21	14512.0
22	15268.0
23	17147.0
24	20230.0
25	25011.0
26	31240.5
27	38317.5
28	46224.0
29	55050.0
30	63664.0
31	71737.5
32	80003.0
33	88006.5
34	95977.5
35	103998.5
36	112444.5
37	120538.0
38	127423.0
39	132983.5
40	139177.5
41	144929.5
42	148445.5
43	152882.0
44	157364.0
45	160263.0
46	162323.0
47	163803.0
48	164143.5
49	163044.0
50	161773.5
51	159307.0
52	153631.5
53	147242.0
54	140145.5
55	131141.0
56	121670.0
57	111906.0
58	101189.5
59	88234.5
60	74602.0
61	61839.0
62	49872.5
63	38921.5
64	29375.0
65	22172.0
66	16786.5
67	12742.5
68	9654.5
69	7078.0
70	5125.0
71	3677.5
72	2535.0
73	1703.5
74	1127.5
75	759.5
76	531.5
77	362.0
78	241.0
79	166.5
80	121.0
81	78.5
82	51.0
83	39.0
84	28.5
85	19.5
86	15.5
87	13.0
88	9.5
89	6.0
90	3.5
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	2.124212249163724E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0012612510229409613
20-21	0.00252258376305769
22-23	0.003571754354121254
24-25	7.835471044614377E-4
26-27	0.0012354985023898262
28-29	0.0031637837684067743
30-31	0.005990721570431716
32-33	0.01087897586522564
34-35	1.875700540657283E-4
36-37	6.062564861023784E-4
38-39	0.0014933764004985705
40-41	0.0020396589087989104
42-43	3.176066927187009E-4
44-45	2.7894920948583525E-5
46-47	0.0028919090450874015
48-49	0.0027249371409788317
50-51	6.489181309023611E-4
52-53	9.905742490026884E-4
54-55	0.0010156525302554057
56-57	0.0011315572252311055
58-59	5.42358009919728E-4
60-61	3.201543570873637E-4
62-63	0.0014649581245575248
64-65	2.808022082285655E-4
66-67	0.0016587392097039719
68-69	9.602661089441107E-4
70-71	4.5408120074637976E-4
72-73	3.6142539604830615E-4
74-75	0.0056075571900114935
76-77	0.002124663805677574
78-79	0.0052143695716062016
80-81	1.387499943632815E-4
82-83	0.004205562251999402
84-85	0.002356346748562807
86-87	5.979023411681489E-4
88-89	0.002522393143509441
90-91	5.808026730412571E-4
92-93	1.908225788908247E-5
94-95	5.649234363162784E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	86.0
20-21	245.0
22-23	585.0
24-25	1142.0
26-27	1931.0
28-29	4127.0
30-31	9409.0
32-33	12600.0
34-35	18409.0
36-37	26889.0
38-39	30636.0
40-41	31058.0
42-43	34426.0
44-45	40002.0
46-47	40113.0
48-49	38251.0
50-51	35312.0
52-53	34841.0
54-55	38056.0
56-57	39321.0
58-59	39439.0
60-61	37629.0
62-63	36923.0
64-65	39275.0
66-67	41035.0
68-69	41127.0
70-71	40018.0
72-73	38627.0
74-75	39625.0
76-77	39923.0
78-79	41653.0
80-81	41835.0
82-83	40511.0
84-85	40976.0
86-87	42914.0
88-89	46407.0
90-91	48122.0
92-93	51603.0
94-95	60656.0
96-97	136633.0
98-99	780857.0
100-101	1602875.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.24336079858728
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.64699283275293	85.44666354741591
2	4.875403936495037	8.896964808329386
3	0.8978056777213367	2.4575642213804425
4	0.28305973679748697	1.03309286768665
5	0.11811078735728185	0.5388412592522843
6	0.05698926559420884	0.31199352733553476
7	0.03450493682587097	0.22038424800947834
8	0.021495102062151532	0.15690282822874796
9	0.013879246906657233	0.11397502198051221
>10	0.05037444524757814	0.6960805788778107
>50	8.094387648596669E-4	0.04846350739894132
>100	5.74593474630745E-4	0.07907358410430945
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	7.965795934363966E-5	0.0	0.0	0.0
3	0.0	7.965795934363966E-5	0.0	0.0	0.0
4	0.0	1.062106124581862E-4	0.0	0.0	0.0
5	0.0	1.062106124581862E-4	0.0	0.0	0.0
6	0.0	1.062106124581862E-4	0.0	0.0	0.0
7	0.0	1.062106124581862E-4	0.0	0.0	0.0
8	0.0	1.3276326557273277E-4	0.0	0.0	0.0
9	0.0	1.8586857180182586E-4	0.0	2.655265311454655E-5	0.0
10-11	0.0	1.8586857180182586E-4	0.0	2.655265311454655E-5	0.0
12-13	0.0	1.8586857180182586E-4	0.0	7.965795934363966E-5	0.0
14-15	0.0	2.6552653114546553E-4	0.0	7.965795934363966E-5	0.0
16-17	0.0	2.6552653114546553E-4	0.0	7.965795934363966E-5	2.655265311454655E-5
18-19	0.0	2.788028577027388E-4	0.0	7.965795934363966E-5	2.655265311454655E-5
20-21	0.0	3.1863183737455863E-4	0.0	1.062106124581862E-4	2.655265311454655E-5
22-23	0.0	3.9828979671819827E-4	0.0	1.725922452445526E-4	5.31053062290931E-5
24-25	0.0	5.576057154054776E-4	0.0	1.8586857180182586E-4	5.31053062290931E-5
26-27	0.0	6.372636747491173E-4	0.0	1.8586857180182586E-4	5.31053062290931E-5
28-29	0.0	6.372636747491173E-4	0.0	4.248424498327449E-4	5.31053062290931E-5
30-31	0.0	6.638163278636639E-4	0.0	0.001593159186872793	5.31053062290931E-5
32-33	0.0	6.638163278636639E-4	0.0	0.0040758322530828955	5.31053062290931E-5
34-35	0.0	7.301979606500301E-4	0.0	0.007527677157973948	5.31053062290931E-5
36-37	0.0	7.965795934363965E-4	0.0	0.012731997168425071	5.31053062290931E-5
38-39	0.0	7.965795934363965E-4	0.0	0.01974189759066536	7.965795934363966E-5
40-41	0.0	9.293428590091293E-4	0.0	0.026884561278478384	7.965795934363966E-5
42-43	0.0	9.691718386809491E-4	0.0	0.034133435578749594	7.965795934363966E-5
44-45	0.0	0.0010621061245818621	0.0	0.04241786335048811	9.293428590091293E-5
46-47	0.0	0.0011417640839255017	0.0	0.051724568267136686	1.062106124581862E-4
48-49	0.0	0.001155040410482775	0.0	0.06121714175558708	1.5931591868727931E-4
50-51	0.0	0.0012745273494982345	0.0	0.06979364871158561	1.5931591868727931E-4
52-53	0.0	0.0013010800026127811	0.0	0.07807807648332414	1.9914489835909914E-4
54-55	0.0	0.001340908982284601	0.0	0.08701304425636905	2.3897387803091896E-4
56-57	0.0	0.001354185308841874	0.0	0.09560282753892486	2.3897387803091896E-4
58-59	0.0	0.001354185308841874	0.0	0.10395363694344975	2.3897387803091896E-4
60-61	0.0	0.001354185308841874	0.0	0.11230444634797465	2.3897387803091896E-4
62-63	0.0	0.001354185308841874	0.0	0.12100044024298864	2.3897387803091896E-4
64-65	0.0	0.0014603959213000603	0.0	0.1288600255648944	2.3897387803091896E-4
66-67	0.0	0.001486948574414607	0.0	0.1368125451727011	2.3897387803091896E-4
68-69	0.0	0.001526777554086427	0.0	0.145017314985096	2.3897387803091896E-4
70-71	0.0	0.0015400538806437	0.0	0.15251843948995542	2.3897387803091896E-4
72-73	0.0	0.0015400538806437	0.0	0.158280365215812	2.6552653114546553E-4
74-75	0.0	0.001593159186872793	0.0	0.16271465828594128	2.920791842600121E-4
76-77	0.0	0.0016595408196591597	0.0	0.1673215436013151	2.920791842600121E-4
78-79	0.0	0.001672817146216433	0.0	0.17017595381112885	2.920791842600121E-4
80-81	0.0	0.001672817146216433	0.0	0.17126461258882525	2.920791842600121E-4
82-83	0.0	0.001686093472773706	0.0	0.1719948105494753	2.920791842600121E-4
84-85	0.0	0.0016993697993309793	0.0	0.17247275830553713	2.920791842600121E-4
86-87	0.0	0.0016993697993309793	0.0	0.17273828483668258	2.920791842600121E-4
88	0.0	0.0017524751055600725	0.0	0.17280466646946896	2.920791842600121E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3810	0.0	45.631695	1
GTATCAA	6710	0.0	33.093704	1
TCAACGC	7665	0.0	27.680515	4
ATCAACG	7785	0.0	27.35968	3
TACGCTA	180	7.844495E-4	26.990936	94
CAACGCA	7960	0.0	26.654667	5
TATCAAC	8135	0.0	26.385134	2
AACGCAG	8160	0.0	26.203318	6
ACGCAGA	9680	0.0	22.00363	7
CGCAGAG	9925	0.0	21.501976	8
GTACATG	9760	0.0	21.274523	1
TACATGG	10110	0.0	20.497267	2
GTGGTAT	2070	0.0	20.300604	1
ACATGGG	10005	0.0	20.135893	3
GCAGAGT	11965	0.0	17.870378	9
TGGTATC	2070	0.0	17.514246	2
CATGGGG	7515	0.0	17.323563	4
GAGTACT	6770	0.0	16.94789	12-13
GGACCGT	475	0.0	16.479313	6
AGAGTAC	10500	0.0	16.420458	10-11
>>END_MODULE