FastQCFastQC Report
Fri 27 May 2016
SRR2138710_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138710_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9601958
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106200.047.013091
GTATCAA185850.032.9471781
TCAACGC221700.026.9543694
ATCAACG226300.026.4245663
CAACGCA228950.026.1187135
TATCAAC236850.025.5242232
AACGCAG235250.025.4714856
GTACATG260650.021.2765161
ACGCAGA283900.021.0633327
TACATGG259500.020.89732
CGCAGAG289750.020.6663368
ACATGGG268900.019.7859973
GTGGTAT56500.019.2829191
TGGTATC54600.018.603672
CATGGGG207950.017.8052924
GCAGAGT335850.017.5734989
GAGTACT200300.017.04415712-13
AGAGTAC299400.016.60074410-11
CAGAGTA328050.016.5118310-11
GAGTACA194400.015.6753141
Produced by FastQC (version 0.11.5)