##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138710_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9601958
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.447699208848864	31.0	31.0	34.0	30.0	34.0
2	31.738296397463934	31.0	31.0	34.0	30.0	34.0
3	31.61759080804144	31.0	31.0	34.0	30.0	34.0
4	35.55556575023552	37.0	35.0	37.0	33.0	37.0
5	35.29090098082079	37.0	35.0	37.0	33.0	37.0
6	35.398139733583506	37.0	35.0	37.0	33.0	37.0
7	35.306265034693965	37.0	35.0	37.0	32.0	37.0
8	35.28909114161924	37.0	35.0	37.0	32.0	37.0
9	36.97401175885168	39.0	37.0	39.0	33.0	39.0
10-11	36.822296608670854	39.0	37.0	39.0	32.5	39.0
12-13	36.77584342693439	39.0	37.0	39.0	32.0	39.0
14-15	37.86263655808534	40.0	37.0	41.0	32.5	41.0
16-17	37.79753577343288	40.0	37.0	41.0	32.0	41.0
18-19	37.789973722026275	40.0	37.0	41.0	32.0	41.0
20-21	37.786567441242056	40.0	37.0	41.0	32.0	41.0
22-23	37.70708282123387	39.5	37.0	41.0	32.0	41.0
24-25	37.61107147859336	39.0	36.5	41.0	32.0	41.0
26-27	37.405727239951084	39.0	36.0	41.0	32.0	41.0
28-29	37.22515828798914	39.0	36.0	40.0	31.0	41.0
30-31	37.04195954484197	39.0	36.0	40.0	31.0	41.0
32-33	36.85451258731644	39.0	35.5	40.0	30.0	41.0
34-35	36.7042439174562	38.0	35.0	40.0	30.0	41.0
36-37	36.81259034459595	39.0	35.0	40.0	30.0	41.0
38-39	36.78036723326912	39.0	35.0	40.0	30.0	41.0
40-41	36.73861205119971	39.0	35.0	40.0	30.0	41.0
42-43	36.82287106930364	39.0	35.0	40.0	30.0	41.0
44-45	36.8659476024561	39.0	35.0	40.0	30.0	41.0
46-47	36.733803529324575	39.0	35.0	40.0	30.0	41.0
48-49	36.66514217883117	39.0	35.0	40.0	30.0	41.0
50-51	36.56248102938987	39.0	35.0	40.0	30.0	41.0
52-53	36.39732547881286	38.0	35.0	40.0	30.0	41.0
54-55	36.214133511537526	38.0	34.5	40.0	29.0	41.0
56-57	36.07220667831065	38.0	34.0	40.0	29.0	41.0
58-59	35.80006193587725	38.0	34.0	40.0	28.0	41.0
60-61	35.649732786490496	38.0	34.0	40.0	28.0	41.0
62-63	35.39219853506178	37.0	34.0	40.0	28.0	41.0
64-65	35.079753773909474	37.0	33.0	39.5	28.0	41.0
66-67	34.74449089472597	36.0	33.0	39.0	27.5	40.5
68-69	34.52618167235917	36.0	33.0	39.0	27.0	40.0
70-71	34.19983589016832	35.0	32.0	38.5	26.5	40.0
72-73	33.9102116446863	35.0	32.0	38.0	26.0	40.0
74-75	33.63302787308243	35.0	32.0	37.0	26.0	39.5
76-77	32.35422847236231	34.0	30.5	36.0	25.5	38.5
78-79	33.1691941240059	34.5	31.5	36.0	26.0	39.0
80-81	33.232577109389965	35.0	32.0	36.0	27.0	38.5
82-83	32.99051076957489	35.0	32.0	36.0	26.0	37.0
84-85	32.77891687231531	34.0	32.0	35.0	26.0	37.0
86-87	32.5972728540747	34.0	31.5	35.0	26.0	36.5
88-89	32.47292318355073	34.0	31.0	35.0	26.0	36.0
90-91	32.32513850449284	34.0	31.0	35.0	26.0	36.0
92-93	32.32643085228919	34.0	31.0	35.0	26.0	35.5
94-95	32.26833689115916	34.0	31.0	35.0	26.0	35.0
96-97	32.408278267588784	34.0	31.0	35.0	27.0	35.0
98-99	32.5873993505069	34.0	31.5	35.0	28.0	35.0
100	31.373763512765752	33.0	30.0	34.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	2.0
18	3.0
19	16.0
20	127.0
21	731.0
22	3353.0
23	10618.0
24	27440.0
25	56208.0
26	98931.0
27	150810.0
28	208950.0
29	268177.0
30	330457.0
31	396486.0
32	476242.0
33	577146.0
34	723197.0
35	935973.0
36	1292166.0
37	1756336.0
38	1621637.0
39	654712.0
40	12239.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.94796082215731	20.95684026112174	14.123202788431277	23.971996128289668
2	15.842716662580694	21.77886010332476	39.296172718106035	23.08225051598851
3	18.79661035307017	25.770062812280514	30.6133737084907	24.81995312615862
4	13.368252600146763	17.800379880853466	37.77348328330534	31.057884235694427
5	13.274552960969002	37.85625806736501	35.54673952958344	13.322449442082544
6	31.02881724748223	37.639437706351146	17.883706635667433	13.44803841049919
7	27.056919015892383	32.381687151724684	22.716460538569322	17.844933293813618
8	24.971885942429658	36.660220759141	20.6839792467328	17.68391405169654
9	25.49812236212656	16.338896712524676	21.121452520413026	37.04152840493574
10-11	24.687568931253395	26.619201000462617	28.573666954177472	20.119563114106516
12-13	25.04267879530404	24.09170088017465	28.931979290057296	21.933641034464014
14-15	22.10955307240461	25.676190210371676	26.340752584004225	25.873504133219495
16-17	21.89504473983327	28.806603819762593	27.14476568216607	22.15358575823806
18-19	21.87048978077666	28.008439245700234	28.785850286914773	21.33522068660833
20-21	22.61394207985502	27.1653395686975	28.768501689901733	21.452216661545755
22-23	22.34781494070114	27.070061454931132	28.581071793708023	22.001051810659707
24-25	22.107872172300553	27.239708742977886	28.660514350677424	21.991904734044134
26-27	22.084565509790437	27.365490795315417	28.599370940372676	21.950572754521467
28-29	22.09247134066864	27.45932163351198	28.568048669631896	21.880158356187483
30-31	22.263167985572434	27.37921282862973	28.47015344393027	21.887465741867576
32-33	22.100361301271516	27.52340723281712	28.38501413049357	21.991217335417794
34-35	22.245428254925745	27.522257768258314	28.407655641317238	21.824658335498707
36-37	22.235658727554245	27.468371058388257	28.33438198385775	21.961588230199748
38-39	22.078034804277458	27.60365224556117	28.392166246452515	21.926146703708852
40-41	22.349132685889934	27.49346958875482	28.21416737619589	21.943230349159357
42-43	22.165000571758206	27.607872755007822	28.06440553618247	22.1627211370515
44-45	22.28131171693796	27.69609753542266	27.875666141825572	22.146924605813805
46-47	22.36452624890244	27.684156496552344	27.664319186920146	22.28699806762507
48-49	22.25525669591378	27.797916889873626	27.673389178005298	22.2734372362073
50-51	22.278112998086765	27.890876326173508	27.57307016577627	22.257940509963454
52-53	22.43539884987033	27.797844690875745	27.440279796954897	22.326476662299026
54-55	22.40221927304915	27.70423705409063	27.52311418748976	22.37042948537046
56-57	22.388474146374804	27.822702698386127	27.492777228730574	22.296045926508498
58-59	22.349527603388474	27.875561012585514	27.512448321218287	22.26246306280773
60-61	22.40849448199495	27.823439724365112	27.57462072703768	22.19344506660226
62-63	22.456928859022472	27.892800742854927	27.450007222471363	22.200263175651234
64-65	22.36594399500613	27.832652585460142	27.49206393430992	22.309339485223806
66-67	22.4222182651065	27.86349041715585	27.36415682195334	22.350134495784307
68-69	22.34697344435575	27.84038559148821	27.495722985623594	22.316917978532445
70-71	22.33341902250876	27.60993876242991	27.53488044047576	22.521761774585567
72-73	22.41322436147224	27.699637676769534	27.44787703288138	22.439260928876852
74-75	22.229790767553315	27.826262188867783	27.513035375416106	22.430911668162796
76-77	22.378966565194762	27.721092685748154	27.436777944042532	22.463162805014548
78-79	22.286836570193255	27.726219122945732	27.4591486001713	22.52779570668971
80-81	22.331854405170137	27.72046088848139	27.45648761445438	22.491197091894087
82-83	22.332035084661875	27.716880071748314	27.552647600861313	22.3984372427285
84-85	22.40866678395383	27.722467641054173	27.476221692724994	22.392643882267002
86-87	22.304376695384544	27.721053165109943	27.563601518878656	22.410968620626857
88-89	22.322673803647078	27.633795807019073	27.609280818161107	22.434249571172742
90-91	22.34785907248221	27.695145929955284	27.576989014455144	22.380005983107367
92-93	22.3802457342789	27.627327376439048	27.60734148813329	22.385085401148757
94-95	22.157238504720414	27.676024932204196	27.763585804361746	22.403150758713643
96-97	22.184102579381175	27.836245166946018	27.82444339154635	22.15520886212646
98-99	23.29497455292499	29.829747887987086	27.94950654229089	18.92577101679703
100	22.378603894753283	30.901074742185102	21.586420087389026	25.13390127567259
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1706.0
1	1596.5
2	2326.5
3	3378.5
4	4525.5
5	7260.5
6	9839.5
7	11974.5
8	13534.5
9	13581.0
10	12250.0
11	10678.0
12	11216.0
13	14898.0
14	23493.5
15	35000.5
16	44628.5
17	50488.5
18	52685.0
19	52316.0
20	50348.0
21	47974.5
22	47043.5
23	50048.5
24	57845.0
25	70599.5
26	87537.5
27	106281.0
28	125532.0
29	147057.5
30	169365.5
31	190578.0
32	211671.5
33	232034.0
34	252820.0
35	273088.5
36	295598.0
37	315598.0
38	328970.0
39	341092.0
40	353474.0
41	364492.0
42	373749.0
43	383495.5
44	392445.0
45	400507.5
46	405212.0
47	408114.5
48	410640.5
49	409026.0
50	405154.0
51	396106.5
52	382383.0
53	366472.5
54	347726.0
55	326272.5
56	302693.0
57	278056.5
58	249558.0
59	217827.0
60	185820.0
61	154456.0
62	124826.0
63	98972.5
64	76957.0
65	59851.5
66	46738.0
67	36057.0
68	27274.0
69	20284.0
70	14864.0
71	10490.0
72	7391.5
73	5000.0
74	3384.0
75	2284.0
76	1535.0
77	1049.0
78	735.0
79	518.0
80	341.0
81	218.5
82	159.5
83	118.0
84	92.0
85	73.5
86	53.0
87	37.5
88	26.5
89	20.0
90	17.0
91	13.5
92	10.0
93	5.0
94	1.0
95	0.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	1.4580359547500626E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0015830104651572106
20-21	0.002838121122156639
22-23	0.004796826532065249
24-25	0.0010107127739246524
26-27	0.0014908133319787437
28-29	0.004168247583681482
30-31	0.007503344239482504
32-33	0.013413889327867733
34-35	1.6832030849746142E-4
36-37	9.363965598165891E-4
38-39	0.0021646038329802345
40-41	0.0025595094001208993
42-43	2.822149291371881E-4
44-45	3.2911118423474054E-5
46-47	0.003214506875213953
48-49	0.0038033861710002424
50-51	9.259603259516685E-4
52-53	0.0010160762785109856
54-55	9.401053649201772E-4
56-57	0.0013211406340699974
58-59	5.944153960245023E-4
60-61	4.3325006507777015E-4
62-63	0.001510241017415271
64-65	4.314819327111323E-4
66-67	0.0018425023955654027
68-69	0.0015958399744868252
70-71	4.817584104901545E-4
72-73	5.73231122750019E-4
74-75	0.007766824603107667
76-77	0.0028475555444184494
78-79	0.005765931990832168
80-81	1.2432982769267325E-4
82-83	0.004367171257497474
84-85	0.0025835815391537145
86-87	6.027029411540454E-4
88-89	0.002908849749768606
90-91	5.67295204540177E-4
92-93	4.646078036455916E-5
94-95	4.5368121715188805E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	327.0
20-21	1084.0
22-23	2284.0
24-25	4704.0
26-27	6780.0
28-29	11939.0
30-31	24856.0
32-33	33432.0
34-35	49319.0
36-37	69167.0
38-39	79686.0
40-41	83468.0
42-43	93048.0
44-45	108236.0
46-47	108054.0
48-49	100360.0
50-51	92869.0
52-53	92005.0
54-55	99171.0
56-57	103780.0
58-59	102822.0
60-61	99698.0
62-63	98017.0
64-65	104244.0
66-67	109384.0
68-69	111377.0
70-71	109948.0
72-73	104670.0
74-75	108493.0
76-77	106787.0
78-79	114077.0
80-81	117820.0
82-83	114884.0
84-85	118738.0
86-87	126689.0
88-89	141802.0
90-91	151094.0
92-93	167978.0
94-95	198525.0
96-97	400535.0
98-99	633515.0
100-101	5096292.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.2154780857268
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.07876672811447	76.76105172108936
2	6.72085953531701	11.454425168981093
3	1.774857717508507	4.5373604679488775
4	0.6555335568749835	2.234464218013548
5	0.3098941554896313	1.3203889308010743
6	0.1479466982896408	0.7564409175573912
7	0.09529659110041024	0.5684521199393033
8	0.05595351346712942	0.3814484320542069
9	0.04641226743225833	0.35595392024542516
>10	0.11254632758691464	1.4556286965712448
>50	0.0015474238223731319	0.08871945031376252
>100	3.4597669223969114E-4	0.05768852136914985
>500	3.950830435996832E-5	0.027977435115584013
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0829085067858035E-5
2	0.0	1.0414542533929017E-5	0.0	2.0829085067858035E-5	2.0829085067858035E-5
3	0.0	1.0414542533929017E-5	0.0	2.0829085067858035E-5	2.0829085067858035E-5
4	0.0	7.290179773750312E-5	0.0	3.1243627601787054E-5	2.0829085067858035E-5
5	0.0	7.290179773750312E-5	0.0	3.1243627601787054E-5	3.1243627601787054E-5
6	0.0	7.290179773750312E-5	0.0	3.1243627601787054E-5	3.1243627601787054E-5
7	0.0	7.290179773750312E-5	0.0	3.1243627601787054E-5	4.165817013571607E-5
8	0.0	8.331634027143214E-5	0.0	3.1243627601787054E-5	5.2072712669645085E-5
9	0.0	2.0829085067858034E-4	0.0	6.248725520357411E-5	1.0414542533929017E-4
10-11	2.0829085067858035E-5	2.0829085067858034E-4	0.0	7.810906900446762E-5	1.5101086674197076E-4
12-13	4.165817013571607E-5	2.0829085067858034E-4	0.0	8.331634027143214E-5	1.8746176561072232E-4
14-15	4.686544140268058E-5	2.8639991968304793E-4	0.0	9.373088280536116E-5	2.343272070134029E-4
16-17	6.248725520357411E-5	2.9160719095001246E-4	0.0	1.0414542533929017E-4	2.4994902081429643E-4
18-19	7.290179773750312E-5	3.020217334839415E-4	0.0	1.1455996787321919E-4	2.655708346151899E-4
20-21	7.290179773750312E-5	3.17643547284835E-4	0.0	1.1455996787321919E-4	2.9160719095001246E-4
22-23	1.4059632420804173E-4	3.593017174205511E-4	0.0	1.4059632420804173E-4	3.0722900475090606E-4
24-25	2.1870539321250935E-4	4.217889726241252E-4	0.0	1.7183995180982878E-4	3.2285081855179954E-4
26-27	2.2911993574643838E-4	4.4782532895894776E-4	0.0	2.343272070134029E-4	3.2285081855179954E-4
28-29	2.655708346151899E-4	4.4782532895894776E-4	0.0	5.155198554294863E-4	3.4367990361965756E-4
30-31	3.1243627601787054E-4	5.311416692303799E-4	0.0	0.0014996941248857786	3.5409444615358657E-4
32-33	3.1243627601787054E-4	5.311416692303799E-4	0.0	0.0035669808178706887	3.8533807375537365E-4
34-35	3.1243627601787054E-4	6.248725520357411E-4	0.0	0.006352870945696701	4.322035151580542E-4
36-37	3.4367990361965756E-4	7.342252486419957E-4	0.0	0.01060200429953974	4.686544140268058E-4
38-39	3.5409444615358657E-4	7.915052325786053E-4	0.0	0.017137129739580197	4.894834990946638E-4
40-41	3.6450898868751563E-4	8.69614301583073E-4	0.0	0.023984691455638526	4.894834990946638E-4
42-43	3.801308024884092E-4	9.11272471718789E-4	0.0	0.030556267794547738	4.894834990946638E-4
44-45	3.8533807375537365E-4	0.0010258324395920082	0.0	0.038039116605175734	5.103125841625218E-4
46-47	3.8533807375537365E-4	0.0011143560511304048	0.0	0.046391579717386805	5.207271266964509E-4
48-49	3.9054534502233813E-4	0.0011195633223973693	0.0	0.05456699560652109	5.259343979634154E-4
50-51	4.0616715882323167E-4	0.0012185014764696952	0.0	0.0628101060221259	5.311416692303799E-4
52-53	4.165817013571607E-4	0.001239330561537553	0.0	0.0712458854746084	5.571780255652025E-4
54-55	4.4782532895894776E-4	0.001260159646605411	0.0	0.07927549776826769	6.04043466967883E-4
56-57	4.8427622782769926E-4	0.0012914032742071982	0.0	0.08746653547120285	6.509089083705636E-4
58-59	5.051053128955574E-4	0.0013018178167411272	0.0	0.09575651132821035	6.873598072393151E-4
60-61	5.207271266964509E-4	0.0013643050719447012	0.0	0.1035986618562589	6.977743497732442E-4
62-63	5.259343979634154E-4	0.0013747196144786302	0.0	0.11089404890127619	7.498470624428893E-4
64-65	5.415562117643089E-4	0.0014319995984152399	0.0	0.11792386511167827	7.602616049768182E-4
66-67	5.51970754298238E-4	0.0014580359547500625	0.0	0.12557334660284913	7.654688762437828E-4
68-69	5.51970754298238E-4	0.001515315938686672	0.0	0.13309785358361284	7.862979613116408E-4
70-71	5.51970754298238E-4	0.0015621813800893525	0.0	0.13937261546030508	7.915052325786053E-4
72-73	5.675925680991314E-4	0.0015725959226232816	0.0	0.14441846131799368	8.227488601803924E-4
74-75	5.832143819000249E-4	0.0016194613640259621	0.0	0.14849054744875995	8.383706739812859E-4
76-77	5.832143819000249E-4	0.001707984975564359	0.0	0.15184403014468506	8.435779452482504E-4
78-79	5.93628924433954E-4	0.0017288140606322169	0.0	0.15458826210237536	8.435779452482504E-4
80-81	5.93628924433954E-4	0.0017288140606322169	0.0	0.15556202182929774	8.539924877821795E-4
82-83	5.93628924433954E-4	0.0017288140606322169	0.0	0.15639518523201207	8.69614301583073E-4
84-85	5.93628924433954E-4	0.0017288140606322169	0.0	0.1566711706091612	8.800288441170019E-4
86-87	5.93628924433954E-4	0.0017600576882340038	0.0	0.15684821783223796	8.90443386650931E-4
88	5.93628924433954E-4	0.001832959485971507	0.0	0.1568742541885728	9.060652004518245E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10620	0.0	47.01309	1
GTATCAA	18585	0.0	32.947178	1
TCAACGC	22170	0.0	26.954369	4
ATCAACG	22630	0.0	26.424566	3
CAACGCA	22895	0.0	26.118713	5
TATCAAC	23685	0.0	25.524223	2
AACGCAG	23525	0.0	25.471485	6
GTACATG	26065	0.0	21.276516	1
ACGCAGA	28390	0.0	21.063332	7
TACATGG	25950	0.0	20.8973	2
CGCAGAG	28975	0.0	20.666336	8
ACATGGG	26890	0.0	19.785997	3
GTGGTAT	5650	0.0	19.282919	1
TGGTATC	5460	0.0	18.60367	2
CATGGGG	20795	0.0	17.805292	4
GCAGAGT	33585	0.0	17.573498	9
GAGTACT	20030	0.0	17.044157	12-13
AGAGTAC	29940	0.0	16.600744	10-11
CAGAGTA	32805	0.0	16.51183	10-11
GAGTACA	19440	0.0	15.675314	1
>>END_MODULE