FastQCFastQC Report
Fri 27 May 2016
SRR2138709_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138709_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4745950
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52450.043.104821
GTATCAA90150.033.6355441
TCAACGC104400.028.140534
ATCAACG107050.027.4055883
CAACGCA107650.027.4053045
AACGCAG110550.026.6492796
TATCAAC111450.026.5445232
GTACATG139200.022.2255151
ACGCAGA132500.022.1416477
TACATGG136600.021.777452
CGCAGAG136850.021.467828
ACATGGG140650.020.8878153
GTGGTAT28450.020.1913571
CATGGGG107350.018.3849624
GCAGAGT161050.018.1400729
GAGTACT87600.017.19086512-13
TGGTATC30800.016.6524832
CAGAGTA152950.016.63266810-11
AGAGTAC140200.016.57950810-11
GAGTACA96600.015.8435131
Produced by FastQC (version 0.11.5)