FastQCFastQC Report
Fri 27 May 2016
SRR2138708_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138708_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6172788
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72350.047.4181251
GTATCAA127250.034.327961
TCAACGC152450.027.9460494
ATCAACG154550.027.6200083
CAACGCA157750.027.0597295
TATCAAC161200.026.866612
AACGCAG161350.026.4559766
ACGCAGA190600.022.3742077
GTACATG186550.021.9482211
CGCAGAG196500.021.6813018
TACATGG184000.021.621122
ACATGGG188150.020.504833
GTGGTAT36550.018.613751
GAGTACT128000.018.53832612-13
CATGGGG141650.018.3623564
GCAGAGT230700.018.3233139
TGGTATC36700.017.0682242
AGAGTAC207050.017.000210-11
CAGAGTA225800.016.79189510-11
GTACTTT140950.016.68975414-15
Produced by FastQC (version 0.11.5)