##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138708_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6172788
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.803425453781987	31.0	31.0	34.0	30.0	34.0
2	32.076979316315416	33.0	31.0	34.0	30.0	34.0
3	31.9878497366182	33.0	31.0	34.0	30.0	34.0
4	35.830536703998256	37.0	35.0	37.0	35.0	37.0
5	35.61968805667715	37.0	35.0	37.0	33.0	37.0
6	35.70774437741908	37.0	35.0	37.0	33.0	37.0
7	35.62738846692937	37.0	35.0	37.0	33.0	37.0
8	35.610196559480094	37.0	35.0	37.0	33.0	37.0
9	37.33844998402667	39.0	37.0	39.0	34.0	39.0
10-11	37.215309354541255	39.0	37.0	39.0	33.0	39.0
12-13	37.17771807487962	39.0	37.0	39.0	33.0	39.0
14-15	38.37960942446104	40.0	38.0	41.0	33.0	41.0
16-17	38.335308696815765	40.0	38.0	41.0	33.0	41.0
18-19	38.333591725489356	40.0	38.0	41.0	33.5	41.0
20-21	38.329969327212154	40.0	38.0	41.0	33.5	41.0
22-23	38.26584650881959	40.0	38.0	41.0	33.0	41.0
24-25	38.19278110680044	40.0	37.5	41.0	33.0	41.0
26-27	38.0298931342074	40.0	37.0	41.0	33.0	41.0
28-29	37.88371514203605	40.0	37.0	41.0	33.0	41.0
30-31	37.727523767913304	40.0	37.0	41.0	32.5	41.0
32-33	37.565564832815255	39.5	36.5	41.0	32.0	41.0
34-35	37.43918068324401	39.0	36.0	41.0	32.0	41.0
36-37	37.52683362885928	39.0	36.0	41.0	32.0	41.0
38-39	37.50179352472045	39.0	36.0	41.0	32.0	41.0
40-41	37.466219806999014	39.0	36.0	41.0	32.0	41.0
42-43	37.53711572120609	40.0	36.5	41.0	32.0	41.0
44-45	37.57240715256785	40.0	36.5	41.0	32.0	41.0
46-47	37.45952037422303	40.0	36.0	41.0	31.5	41.0
48-49	37.39642345382418	39.0	36.0	41.0	31.0	41.0
50-51	37.30206633528849	39.0	36.0	41.0	31.0	41.0
52-53	37.15170376422871	39.0	36.0	41.0	31.0	41.0
54-55	36.9809977324221	39.0	35.0	41.0	31.0	41.0
56-57	36.84461960548299	39.0	35.0	40.0	31.0	41.0
58-59	36.58878942361865	38.5	35.0	40.0	30.0	41.0
60-61	36.42880367884058	38.0	35.0	40.0	30.0	41.0
62-63	36.16479277703603	38.0	34.5	40.0	30.0	41.0
64-65	35.862415093212945	37.0	34.0	40.0	29.0	41.0
66-67	35.534644015159614	37.0	34.0	39.5	29.0	41.0
68-69	35.30300866457326	36.0	34.0	39.0	29.0	41.0
70-71	34.98005129575844	36.0	34.0	39.0	29.0	40.5
72-73	34.68055493965714	35.0	33.0	38.5	29.0	40.0
74-75	34.39209314187936	35.0	33.0	37.5	28.5	40.0
76-77	33.04523521946652	34.0	31.5	36.0	26.5	39.0
78-79	33.77541464680803	35.0	32.5	36.5	28.5	39.0
80-81	33.79485009809548	35.0	33.0	36.0	29.0	39.0
82-83	33.55712321948671	35.0	33.0	36.0	29.0	37.5
84-85	33.34562762041405	35.0	33.0	35.5	29.0	37.0
86-87	33.16877348807044	35.0	33.0	35.0	29.0	37.0
88-89	33.04936359512986	35.0	32.5	35.0	29.0	36.0
90-91	32.90977551986924	35.0	32.0	35.0	28.5	36.0
92-93	32.89527356340906	34.0	32.0	35.0	29.0	36.0
94-95	32.831015589255415	34.0	32.0	35.0	29.0	35.0
96-97	32.92860433534457	34.0	32.0	35.0	29.0	35.0
98-99	33.047921625260884	34.0	32.0	35.0	29.0	35.0
100	32.00263121026294	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	12.0
20	42.0
21	314.0
22	1239.0
23	4239.0
24	11157.0
25	23999.0
26	43565.0
27	68202.0
28	97234.0
29	129882.0
30	164317.0
31	201560.0
32	247400.0
33	306992.0
34	396268.0
35	531235.0
36	775625.0
37	1198332.0
38	1316297.0
39	632402.0
40	22471.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.95946790980024	19.968108413896605	13.952803822195092	24.119619854108063
2	15.502087549418512	20.830571210286177	40.192519166379924	23.474822073915387
3	18.912300488710823	25.118929569880365	30.084754480605223	25.88401546080358
4	13.204843581214842	17.286710640313583	37.55431095317059	31.954134825300983
5	13.610770368267952	37.50028350236555	35.228376545573894	13.660569583792606
6	31.494925793660823	36.885941976299854	17.922695546971642	13.696436683067684
7	27.652674933919645	32.09426599455546	22.191026161922295	18.0620329096026
8	25.216417605788504	36.545350334403196	20.348584788591477	17.88964727121683
9	25.721732222133664	16.543140635965468	20.862760878876774	36.8723662630241
10-11	25.038645422457407	26.413194491694842	28.09917495951586	20.448985126331895
12-13	25.407822202868463	23.814004304051913	28.539365032461834	22.238808460617797
14-15	22.44668049510205	25.341053345749113	26.059229638212102	26.15303652093673
16-17	22.23988576960686	28.49291924491818	26.775275612899712	22.491919372575246
18-19	22.246442663718632	27.66527751260935	28.473635085059595	21.61464473861242
20-21	22.967438282630777	26.79663941133804	28.439598008609657	21.796324297421528
22-23	22.72748594827559	26.700386862442777	28.281320800927023	22.290806388354607
24-25	22.501767715605798	26.864480106858675	28.31121939882922	22.322532778706318
26-27	22.45532955430849	26.942604712450407	28.28559803380239	22.316467699438707
28-29	22.419734837360316	27.092792320635535	28.254051186027606	22.233421655976542
30-31	22.647198661301058	26.961980459240436	28.13229378795878	22.258527091499726
32-33	22.39292654064545	27.1017320705462	28.128728537760487	22.376612851047863
34-35	22.5728181736486	27.12374500169632	28.11408802672969	22.189348797925394
36-37	22.5686543420908	27.07643112112576	28.047715588886323	22.307198947897113
38-39	22.393149565387223	27.217147191195412	28.091466683828703	22.29823655958866
40-41	22.67481241607692	27.104598126670265	27.900664302922866	22.319925154329944
42-43	22.534218352978204	27.17589584313218	27.76928158244734	22.520604221442277
44-45	22.603102641240035	27.276771374320397	27.61269868012452	22.507427304315048
46-47	22.675899042995738	27.268639911675447	27.402662164602255	22.652798880726564
48-49	22.558816148479863	27.394395806844475	27.3695355792755	22.677252465400166
50-51	22.572401288032605	27.48921636185731	27.32157978529684	22.61680256481325
52-53	22.775287283944078	27.388355777953088	27.177989886142555	22.658367051960276
54-55	22.731094492898464	27.264541150150546	27.267256550881857	22.737107806069126
56-57	22.694692986027082	27.45409989386751	27.241074170572183	22.610132949533227
58-59	22.67639447738763	27.480207611195723	27.20815354577254	22.635244365644105
60-61	22.766299958264344	27.399115070793222	27.277046611727684	22.55753835921475
62-63	22.783403354523244	27.476037950022096	27.187959056435183	22.552599639019473
64-65	22.675672817988694	27.46558382516861	27.197351305536344	22.66139205130635
66-67	22.76571252313859	27.458411769500803	27.05721531737324	22.718660389987367
68-69	22.66672903682367	27.43037568044243	27.16843070583551	22.734464576898382
70-71	22.674153812477712	27.248360151876945	27.21185806041106	22.865627975234283
72-73	22.728343282455786	27.310033322609726	27.143688364094192	22.817935030840296
74-75	22.56523696414046	27.429300427289565	27.187498335336624	22.81796427323335
76-77	22.712648722410076	27.337892267696674	27.10934847513986	22.840110534753393
78-79	22.605669460296735	27.318710035550765	27.146838223672347	22.928782280480156
80-81	22.698681711516087	27.34735955010961	27.11669852637648	22.837260211997823
82-83	22.68422462143854	27.325910921301748	27.24727564880726	22.74258880845245
84-85	22.8023528728724	27.30575413102074	27.170692949230023	22.72120004687683
86-87	22.620313264000153	27.326289587245007	27.259356285832474	22.794040862922365
88-89	22.627134331612748	27.274946531836946	27.29989076223867	22.798028374311635
90-91	22.710962700098452	27.275211309479435	27.2614869807516	22.75233900967051
92-93	22.71027825313916	27.209674051127553	27.31077177405022	22.76927592168307
94-95	22.572031794806307	27.27497625662029	27.39282729639301	22.760164652180396
96-97	22.56975509682969	27.39781704181204	27.468085498872064	22.564342362486205
98-99	23.71642615272741	29.380103728939865	27.624123822999724	19.279346295333
100	22.713747114520878	30.368838331609787	21.279298465179448	25.638116088689888
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1073.0
1	958.5
2	1366.0
3	1991.5
4	2700.0
5	4390.5
6	5909.5
7	7172.5
8	8120.0
9	8146.5
10	7405.5
11	6561.5
12	6976.0
13	9162.5
14	14350.5
15	21469.5
16	27514.0
17	31294.5
18	32546.5
19	31757.5
20	29787.0
21	27746.5
22	26775.0
23	28046.0
24	32126.5
25	39209.0
26	48784.0
27	59272.0
28	70344.0
29	82870.5
30	95387.5
31	107420.0
32	120171.5
33	132471.0
34	144630.5
35	157426.0
36	171761.5
37	184708.0
38	195531.5
39	205308.5
40	215074.5
41	224814.5
42	232208.0
43	239400.0
44	245815.0
45	252530.0
46	258319.0
47	262442.0
48	266496.5
49	268303.0
50	267562.5
51	263162.0
52	255782.5
53	247017.5
54	236882.0
55	224377.0
56	209639.0
57	193295.0
58	174899.0
59	153781.0
60	130776.5
61	108273.0
62	87735.0
63	69676.0
64	53862.5
65	41619.5
66	32146.5
67	24395.0
68	18410.0
69	13649.5
70	9746.0
71	6965.0
72	5068.0
73	3416.5
74	2253.0
75	1528.5
76	1028.5
77	666.0
78	435.5
79	296.0
80	209.5
81	147.0
82	105.5
83	67.0
84	42.0
85	30.0
86	23.5
87	17.5
88	12.5
89	8.0
90	5.5
91	5.0
92	5.0
93	3.5
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	2.7540229795677417E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0014013116925447626
20-21	0.00303763828240753
22-23	0.005606035529141298
24-25	0.0012802910377034365
26-27	0.0017915768648896495
28-29	0.0041293051758528295
30-31	0.008994600314737883
32-33	0.015154277881549277
34-35	2.3713292232473994E-4
36-37	7.892682537809856E-4
38-39	0.001739365087016708
40-41	0.0026639217972770544
42-43	2.864351153276232E-4
44-45	1.7018022170568745E-5
46-47	0.004070764517977159
48-49	0.0030367364185009123
50-51	7.559851387386564E-4
52-53	0.001260244702246333
54-55	0.00109331771382606
56-57	0.0013312798417244114
58-59	7.325288884212913E-4
60-61	3.888399958597799E-4
62-63	0.002095959971655884
64-65	5.579871078497343E-4
66-67	0.0020282330034074314
68-69	0.0019175550127648149
70-71	5.884235896663403E-4
72-73	9.33342097481823E-4
74-75	0.008572595512582923
76-77	0.003918147158076273
78-79	0.006814608623933606
80-81	1.1498542298433167E-4
82-83	0.004919100932530078
84-85	0.0027953292198988735
86-87	6.32883422328018E-4
88-89	0.003482532493192703
90-91	9.149468771677041E-4
92-93	6.911084861786942E-5
94-95	7.421591766509655E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	132.0
20-21	521.0
22-23	1034.0
24-25	2550.0
26-27	3788.0
28-29	7366.0
30-31	15850.0
32-33	20410.0
34-35	29655.0
36-37	42442.0
38-39	49431.0
40-41	51181.0
42-43	56296.0
44-45	65716.0
46-47	64873.0
48-49	59682.0
50-51	55042.0
52-53	53336.0
54-55	57457.0
56-57	60419.0
58-59	60176.0
60-61	57820.0
62-63	56223.0
64-65	59589.0
66-67	63105.0
68-69	64184.0
70-71	63597.0
72-73	60470.0
74-75	62185.0
76-77	61936.0
78-79	66155.0
80-81	67422.0
82-83	65463.0
84-85	67630.0
86-87	71882.0
88-89	80021.0
90-91	84824.0
92-93	92761.0
94-95	109173.0
96-97	240231.0
98-99	401847.0
100-101	3518913.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.8392160102104
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.84432434935256	78.88849905475043
2	6.534110831774297	11.348341239102073
3	1.464834092094441	3.8161513242752867
4	0.5475028146134394	1.901788607376586
5	0.24678155008665192	1.071515816765466
6	0.12251277326624448	0.6383347909006399
7	0.07045126568362264	0.42825528752249814
8	0.04372215716567531	0.3037438280433957
9	0.029675561931190086	0.2319302279490293
>10	0.09444353112960199	1.239018868025927
>50	0.0013834663960562196	0.07770894971415118
>100	2.5760650626367406E-4	0.054712005574488645
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.6200135173927892E-5	0.0	1.6200135173927892E-5	0.0
3	0.0	1.6200135173927892E-5	0.0	1.6200135173927892E-5	0.0
4	0.0	8.100067586963946E-5	0.0	1.6200135173927892E-5	0.0
5	0.0	8.100067586963946E-5	0.0	1.6200135173927892E-5	0.0
6	4.8600405521783676E-5	8.100067586963946E-5	0.0	1.6200135173927892E-5	0.0
7	4.8600405521783676E-5	9.720081104356735E-5	0.0	1.6200135173927892E-5	0.0
8	4.8600405521783676E-5	9.720081104356735E-5	0.0	1.6200135173927892E-5	0.0
9	4.8600405521783676E-5	2.7540229795677417E-4	0.0	1.6200135173927892E-5	1.6200135173927892E-5
10-11	4.8600405521783676E-5	2.7540229795677417E-4	0.0	1.6200135173927892E-5	2.4300202760891838E-5
12-13	4.8600405521783676E-5	2.7540229795677417E-4	0.0	1.6200135173927892E-5	4.050033793481973E-5
14-15	6.480054069571157E-5	4.212035145221252E-4	0.0	3.2400270347855784E-5	6.480054069571157E-5
16-17	1.2150101380445919E-4	4.374036496960531E-4	0.0	3.2400270347855784E-5	6.480054069571157E-5
18-19	1.2960108139142314E-4	4.779039876308728E-4	0.0	3.2400270347855784E-5	6.480054069571157E-5
20-21	1.2960108139142314E-4	5.508045959135483E-4	0.0	3.2400270347855784E-5	6.480054069571157E-5
22-23	1.2960108139142314E-4	5.99405001435332E-4	0.0	3.2400270347855784E-5	8.91007434566034E-5
24-25	1.2960108139142314E-4	7.45206218000683E-4	0.0	9.720081104356735E-5	9.720081104356735E-5
26-27	1.2960108139142314E-4	8.100067586963946E-4	0.0	2.106017572610626E-4	1.2960108139142314E-4
28-29	1.2960108139142314E-4	8.100067586963946E-4	0.0	7.614063531746109E-4	1.3770114897838708E-4
30-31	1.2960108139142314E-4	8.586071642181783E-4	0.0	0.001798215004305996	1.4580121656535103E-4
32-33	1.2960108139142314E-4	8.667072318051422E-4	0.0	0.003863732238981802	1.7010141932624287E-4
34-35	1.2960108139142314E-4	9.558079752617456E-4	0.0	0.006439553731636337	1.8630155450017076E-4
36-37	1.4580121656535103E-4	0.001036808651131385	0.0	0.010926991174814361	2.0250168967409865E-4
38-39	1.4580121656535103E-4	0.0010773089890662047	0.0	0.01824945227342977	2.268018924349905E-4
40-41	1.4580121656535103E-4	0.0011664097325228082	0.0	0.025644813980327853	2.4300202760891838E-4
42-43	1.5390128415231498E-4	0.0011907099352837002	0.0	0.03256227169959506	2.4300202760891838E-4
44-45	1.782014869132068E-4	0.0013851115573708349	0.0	0.04054893834034151	2.4300202760891838E-4
46-47	1.944016220871347E-4	0.0015552129766970776	0.0	0.04888390788732741	2.4300202760891838E-4
48-49	1.944016220871347E-4	0.0015552129766970776	0.0	0.05733227838053081	2.592021627828463E-4
50-51	2.106017572610626E-4	0.0017739148015451043	0.0	0.06561054745440796	2.592021627828463E-4
52-53	2.106017572610626E-4	0.001798215004305996	0.0	0.07331371172961067	2.592021627828463E-4
54-55	2.106017572610626E-4	0.001814415139479924	0.0	0.08119507749172658	2.592021627828463E-4
56-57	2.106017572610626E-4	0.001814415139479924	0.0	0.08818543581927649	2.592021627828463E-4
58-59	2.106017572610626E-4	0.0018468154098277797	0.0	0.09524059468752208	2.592021627828463E-4
60-61	2.4300202760891838E-4	0.0018630155450017076	0.0	0.10238485429922428	2.673022303698102E-4
62-63	3.0780256830462995E-4	0.0018711156125886716	0.0	0.10909171026123042	2.9160243313070206E-4
64-65	3.0780256830462995E-4	0.0020007166939800944	0.0	0.11544216324941015	2.99702500717666E-4
66-67	3.0780256830462995E-4	0.0020250168967409863	0.0	0.1217440158320681	3.0780256830462995E-4
68-69	3.0780256830462995E-4	0.002081717369849734	0.0	0.12841847152372637	3.0780256830462995E-4
70-71	3.2400270347855784E-4	0.00211411764019759	0.0	0.13401561822631847	3.2400270347855784E-4
72-73	4.212035145221252E-4	0.0021303177753715175	0.0	0.1388675587109099	3.564029738264136E-4
74-75	4.212035145221252E-4	0.0021789181808933014	0.0	0.14244778858434795	3.564029738264136E-4
76-77	4.374036496960531E-4	0.002251818789175977	0.0	0.14618191974193834	3.726031090003415E-4
78-79	4.374036496960531E-4	0.0022842190595238326	0.0	0.1485228392745709	3.888032441742694E-4
80-81	4.374036496960531E-4	0.0022842190595238326	0.0	0.1493085458305064	3.9690331176123336E-4
82-83	4.4550371728301703E-4	0.0022842190595238326	0.0	0.1499646513050505	4.050033793481973E-4
84-85	4.53603784869981E-4	0.0022842190595238326	0.0	0.15015095285955066	4.050033793481973E-4
86-87	4.617038524569449E-4	0.0022842190595238326	0.0	0.15026435380576814	4.050033793481973E-4
88	4.8600405521783676E-4	0.0024138201409152557	0.0	0.1502724538733551	4.050033793481973E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7235	0.0	47.418125	1
GTATCAA	12725	0.0	34.32796	1
TCAACGC	15245	0.0	27.946049	4
ATCAACG	15455	0.0	27.620008	3
CAACGCA	15775	0.0	27.059729	5
TATCAAC	16120	0.0	26.86661	2
AACGCAG	16135	0.0	26.455976	6
ACGCAGA	19060	0.0	22.374207	7
GTACATG	18655	0.0	21.948221	1
CGCAGAG	19650	0.0	21.681301	8
TACATGG	18400	0.0	21.62112	2
ACATGGG	18815	0.0	20.50483	3
GTGGTAT	3655	0.0	18.61375	1
GAGTACT	12800	0.0	18.538326	12-13
CATGGGG	14165	0.0	18.362356	4
GCAGAGT	23070	0.0	18.323313	9
TGGTATC	3670	0.0	17.068224	2
AGAGTAC	20705	0.0	17.0002	10-11
CAGAGTA	22580	0.0	16.791895	10-11
GTACTTT	14095	0.0	16.689754	14-15
>>END_MODULE