FastQCFastQC Report
Fri 27 May 2016
SRR2138707_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138707_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5125198
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60150.050.004481
GTATCAA104750.034.063211
TCAACGC128550.026.827184
ATCAACG131700.026.2480983
CAACGCA132850.025.9588585
TATCAAC135100.025.8620052
AACGCAG135350.025.6011476
ACGCAGA161350.021.4246967
GTACATG158150.021.2068841
CGCAGAG164750.020.9825488
TACATGG158750.020.867192
ACATGGG160150.020.4017733
GTGGTAT31250.019.2497251
TGGTATC29950.019.1222782
GAGTACT104150.018.00014112-13
GCAGAGT191250.017.8597249
CATGGGG124050.017.5703564
CAGAGTA185050.017.0999110-11
AGAGTAC170550.016.66936510-11
GTACTTT115750.016.14481514-15
Produced by FastQC (version 0.11.5)