##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138707_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5125198
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.786684338829446	31.0	31.0	34.0	30.0	34.0
2	32.05825589567466	33.0	31.0	34.0	30.0	34.0
3	31.965526405028644	33.0	31.0	34.0	30.0	34.0
4	35.81505865724602	37.0	35.0	37.0	35.0	37.0
5	35.59889159404183	37.0	35.0	37.0	33.0	37.0
6	35.68820482642817	37.0	35.0	37.0	33.0	37.0
7	35.607724618639125	37.0	35.0	37.0	33.0	37.0
8	35.59252676677077	37.0	35.0	37.0	33.0	37.0
9	37.32025689544092	39.0	37.0	39.0	34.0	39.0
10-11	37.1971690654683	39.0	37.0	39.0	33.0	39.0
12-13	37.15464797652696	39.0	37.0	39.0	33.0	39.0
14-15	38.351152774975716	40.0	38.0	41.0	33.0	41.0
16-17	38.308194044405695	40.0	38.0	41.0	33.0	41.0
18-19	38.30453789297506	40.0	38.0	41.0	33.5	41.0
20-21	38.29975426024164	40.0	38.0	41.0	33.5	41.0
22-23	38.23329662503882	40.0	38.0	41.0	33.0	41.0
24-25	38.15414507747755	40.0	37.0	41.0	33.0	41.0
26-27	37.98876641058487	40.0	37.0	41.0	33.0	41.0
28-29	37.84129882503001	40.0	37.0	41.0	33.0	41.0
30-31	37.68489675330947	40.0	37.0	41.0	32.0	41.0
32-33	37.52246073729187	39.0	36.5	41.0	32.0	41.0
34-35	37.393971606805025	39.0	36.0	41.0	31.5	41.0
36-37	37.48221948080348	39.0	36.0	41.0	32.0	41.0
38-39	37.44851989076348	39.0	36.0	41.0	31.5	41.0
40-41	37.41493804359833	39.0	36.0	41.0	31.0	41.0
42-43	37.484923540285976	39.5	36.0	41.0	32.0	41.0
44-45	37.52018717473533	40.0	36.0	41.0	32.0	41.0
46-47	37.39884436861111	39.5	36.0	41.0	31.0	41.0
48-49	37.33494074633914	39.0	36.0	41.0	31.0	41.0
50-51	37.2329722420575	39.0	36.0	41.0	31.0	41.0
52-53	37.076616768471084	39.0	35.0	41.0	31.0	41.0
54-55	36.901015893081045	39.0	35.0	40.5	31.0	41.0
56-57	36.75775155990239	39.0	35.0	40.0	31.0	41.0
58-59	36.49440234948861	38.0	35.0	40.0	30.0	41.0
60-61	36.328516001596725	38.0	35.0	40.0	30.0	41.0
62-63	36.05996532179948	37.5	34.0	40.0	29.5	41.0
64-65	35.74756631916915	37.0	34.0	40.0	29.0	41.0
66-67	35.409243957313706	36.5	34.0	39.0	29.0	41.0
68-69	35.16938224049909	36.0	34.0	39.0	29.0	41.0
70-71	34.842204605017436	36.0	33.5	39.0	29.0	40.0
72-73	34.540653287594935	35.0	33.0	38.0	28.0	40.0
74-75	34.254465875574056	35.0	33.0	37.0	28.0	39.5
76-77	32.91689893138201	34.0	31.5	36.0	26.5	39.0
78-79	33.657180420809205	35.0	32.5	36.0	28.0	39.0
80-81	33.68839529894671	35.0	33.0	36.0	29.0	38.5
82-83	33.459751212244605	35.0	33.0	36.0	29.0	37.0
84-85	33.25993048192268	35.0	33.0	35.0	28.0	37.0
86-87	33.08941752391885	35.0	32.5	35.0	28.0	36.5
88-89	32.97460989800952	35.0	32.0	35.0	28.0	36.0
90-91	32.844184595749155	34.5	32.0	35.0	28.0	36.0
92-93	32.839579886369506	34.0	32.0	35.0	28.5	36.0
94-95	32.78115185187304	34.0	32.0	35.0	29.0	35.0
96-97	32.878240600569	34.0	32.0	35.0	29.0	35.0
98-99	33.00492090714641	34.0	32.0	35.0	29.0	35.0
100	31.95115603808785	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	15.0
20	31.0
21	238.0
22	1047.0
23	3826.0
24	9729.0
25	20683.0
26	37019.0
27	58670.0
28	83614.0
29	110870.0
30	138139.0
31	170270.0
32	209366.0
33	258272.0
34	333324.0
35	447588.0
36	650536.0
37	997808.0
38	1063940.0
39	512154.0
40	18056.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.64563879203082	19.62515782320172	13.4666433309783	24.26256005378915
2	16.01573559026121	20.701194510181757	39.31294738524197	23.970122514315058
3	19.20570749572211	24.723493177813896	29.79103213734515	26.279767189118843
4	13.430661605658942	16.844480935175575	36.96458166104022	32.760275798125264
5	13.778940833115131	37.343552385683445	35.01191563721051	13.865591143990924
6	32.2725482995974	36.31073375116435	17.536239575524693	13.880478373713562
7	28.23408968785206	31.748139291399085	21.802435730287883	18.215335290460974
8	25.74011774764604	36.03593461169695	20.12183724414159	18.102110396515414
9	25.907311288266328	16.19588160301319	20.518856052000334	37.37795105672015
10-11	25.346493930575953	26.147058123412986	27.864191783419876	20.64225616259118
12-13	25.634521827254286	23.555333862223467	28.295004407634593	22.515139902887654
14-15	22.653280907391284	25.080991992894713	25.72932792059936	26.53639917911464
16-17	22.540338929344777	28.252957251602766	26.492537459040605	22.71416636001185
18-19	22.59552004242636	27.424581943567905	28.092059583827915	21.887838430177815
20-21	23.227900693577677	26.527226550788424	28.13338538084721	22.111487374786687
22-23	22.98507212187731	26.437987987753566	28.00987255527991	22.567067335089217
24-25	22.82737629909061	26.56914178770171	28.031673382323174	22.571808530884503
26-27	22.715167610924915	26.707981321505898	28.019078817289078	22.557772250280102
28-29	22.691868421021773	26.775901499081666	28.015742448600374	22.51648763129619
30-31	22.89081956895906	26.65974349349778	27.90439756892872	22.545039368614436
32-33	22.63684800005653	26.85870552197094	27.880838002007618	22.623608475964915
34-35	22.789450720025688	26.877094801494323	27.846393863555107	22.487060614924886
36-37	22.84889905532013	26.780719130086776	27.776957425081577	22.593424389511515
38-39	22.67219423397817	26.91805853845478	27.874787426846893	22.534959800720152
40-41	22.907999237147113	26.81152149398398	27.721540580402227	22.558938688466682
42-43	22.73488000877316	26.932359350217606	27.54937440471476	22.78338623629448
44-45	22.80827210628666	26.995400931795245	27.40833535847712	22.787991603440975
46-47	22.90344786133437	26.98929989819264	27.21595025547345	22.891301984999533
48-49	22.83651409906614	27.092222059202076	27.128738804865833	22.94252503686595
50-51	22.83806718230158	27.20032197632824	27.071671582446555	22.889939258923622
52-53	22.981805656773453	27.130480082390772	26.939751961220402	22.94796229961537
54-55	22.982089147676916	27.024817267098634	27.009249395675166	22.983844189549288
56-57	22.94088595258045	27.20765072342335	26.97004462892782	22.88141869506838
58-59	22.894343732764998	27.19836151443514	26.966800712818824	22.940494039981044
60-61	22.980452231901957	27.1305043817908	27.030477989987716	22.858565396319527
62-63	22.98758171269352	27.280558448542276	26.93089278704668	22.800967051717528
64-65	22.91629971415284	27.190627618750042	26.939021069567083	22.95405159753004
66-67	22.983950933257244	27.175101460451945	26.84523954053777	22.995708065753043
68-69	22.88922114454934	27.178662124008262	26.9333077196707	22.99880901177169
70-71	22.89225865672343	26.976340440721536	27.02099575016983	23.110405152385198
72-73	22.977016866161367	27.052600885921365	26.887321740421317	23.08306050749595
74-75	22.787097504216625	27.19427011840591	26.93895205337616	23.079680324001306
76-77	22.954731700270194	27.066918836364017	26.905380639269794	23.072968824095998
78-79	22.816635236801154	27.055816471397144	26.904938016513324	23.222610275288375
80-81	22.888251020616902	27.077137836625447	26.88742459259282	23.147186550164832
82-83	22.933847040361737	27.085488273388243	27.002028166165733	22.97863652008429
84-85	22.970650418185752	27.047494385505633	26.92279655767153	23.05905863863709
86-87	22.88395501672853	27.080668756363245	27.01792172752527	23.017454499382957
88-89	22.87874562839788	27.047136145996387	26.990916965425953	23.083201260179784
90-91	22.885637151691803	27.022225264682497	27.054734630178945	23.037402953446755
92-93	22.96800525913827	26.94828999526434	27.099395479081167	22.98430926651622
94-95	22.805213429076804	26.99304705335253	27.15707574744647	23.0446637701242
96-97	22.837586742447304	27.167218189262975	27.153370435762543	22.84182463252718
98-99	23.91316110923708	29.164503216105974	27.371069174114776	19.551266500542173
100	22.92201545213121	30.10286828565815	21.036114392419414	25.939001869791227
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1121.0
1	973.0
2	1226.5
3	1710.5
4	2208.5
5	3483.5
6	4790.5
7	6021.0
8	7049.0
9	7194.5
10	6402.0
11	5485.0
12	5635.0
13	7462.5
14	12064.5
15	18075.0
16	22699.0
17	25292.0
18	25961.5
19	25158.5
20	23479.0
21	21630.0
22	20705.0
23	21456.5
24	24467.5
25	29900.5
26	37316.5
27	45340.0
28	53994.0
29	64233.0
30	74169.0
31	83526.0
32	93327.5
33	103119.5
34	113169.5
35	123207.0
36	134621.0
37	145671.5
38	154821.5
39	162943.5
40	171437.5
41	179529.0
42	186365.0
43	194137.5
44	201219.5
45	207786.0
46	213050.5
47	217246.0
48	221700.0
49	224922.5
50	226970.0
51	226945.5
52	223780.5
53	217321.0
54	208692.5
55	198216.5
56	185892.5
57	172334.0
58	156709.0
59	138691.0
60	119513.5
61	100534.5
62	82582.0
63	65983.5
64	50854.5
65	38706.5
66	29608.0
67	22726.0
68	17343.0
69	12890.0
70	9397.0
71	6828.5
72	4876.5
73	3272.0
74	2158.5
75	1432.5
76	961.0
77	672.0
78	480.5
79	347.5
80	233.0
81	154.0
82	108.0
83	65.0
84	37.0
85	23.5
86	19.5
87	16.5
88	13.0
89	10.0
90	7.5
91	6.0
92	4.5
93	2.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.951144131407216E-5
2	1.951144131407216E-5
3	1.5609153051257728E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.001609693908410953
20-21	0.003102461119967001
22-23	0.005376260677024409
24-25	0.0012199488773103268
26-27	0.0017672491819393774
28-29	0.00435794648089959
30-31	0.008436293797161157
32-33	0.014189499887838186
34-35	2.0683274942478335E-4
36-37	8.517070139756207E-4
38-39	0.0018662177365134083
40-41	0.002918481282722842
42-43	3.046226486939304E-4
44-45	2.0516437718609054E-5
46-47	0.0037474333714651114
48-49	0.0036651933079661345
50-51	7.962289746449908E-4
52-53	0.0012439102016936052
54-55	8.666798329777959E-4
56-57	0.001566633124649767
58-59	7.094880389739517E-4
60-61	4.935119442789123E-4
62-63	0.001815174404225726
64-65	4.819366140900595E-4
66-67	0.002067911371177428
68-69	0.001694993061122156
70-71	5.010722947106809E-4
72-73	7.736822317831078E-4
74-75	0.00760422378508137
76-77	0.0032887380454372046
78-79	0.006404688483627716
80-81	1.2769267132174556E-4
82-83	0.004718553208622663
84-85	0.0027894061512194965
86-87	7.885080959066405E-4
88-89	0.0035212225031949315
90-91	9.700057590627639E-4
92-93	4.2421510307578576E-5
94-95	8.105119931893836E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	153.0
20-21	486.0
22-23	902.0
24-25	2065.0
26-27	3180.0
28-29	6521.0
30-31	12695.0
32-33	17260.0
34-35	24710.0
36-37	36618.0
38-39	41861.0
40-41	43363.0
42-43	47414.0
44-45	56818.0
46-47	56798.0
48-49	52635.0
50-51	47737.0
52-53	46346.0
54-55	50355.0
56-57	53626.0
58-59	52771.0
60-61	51336.0
62-63	49230.0
64-65	52743.0
66-67	55578.0
68-69	56742.0
70-71	55972.0
72-73	52847.0
74-75	54270.0
76-77	54115.0
78-79	57689.0
80-81	58916.0
82-83	56951.0
84-85	58256.0
86-87	61902.0
88-89	68120.0
90-91	71672.0
92-93	78356.0
94-95	91292.0
96-97	200171.0
98-99	329327.0
100-101	2855399.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.43986468485889
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.82251769017012	81.20771039541707
2	6.024785034888612	10.656623464818233
3	1.2502100644809429	3.317052267910299
4	0.43645444109399956	1.5439988684583614
5	0.18169762500858763	0.8034656684659861
6	0.10197099857246203	0.5410980789317088
7	0.04606237610801409	0.28516252170390904
8	0.03250991721416605	0.23001381434694484
9	0.024556852648672817	0.19546242528011784
>10	0.0772486790041053	1.0618569309559307
>50	0.0015950738306616209	0.0934500949490888
>100	3.9124697964553417E-4	0.06410546876234867
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.902288262814432E-5
2	0.0	5.8534323942216475E-5	0.0	0.0	3.902288262814432E-5
3	0.0	7.804576525628864E-5	0.0	0.0	3.902288262814432E-5
4	0.0	2.5364873708293807E-4	0.0	0.0	3.902288262814432E-5
5	0.0	2.731601783970102E-4	0.0	1.951144131407216E-5	3.902288262814432E-5
6	1.5609153051257728E-4	2.731601783970102E-4	0.0	1.951144131407216E-5	3.902288262814432E-5
7	1.5609153051257728E-4	2.731601783970102E-4	0.0	3.902288262814432E-5	3.902288262814432E-5
8	1.5609153051257728E-4	2.731601783970102E-4	0.0	5.8534323942216475E-5	3.902288262814432E-5
9	1.5609153051257728E-4	4.87786032851804E-4	0.0	5.8534323942216475E-5	5.8534323942216475E-5
10-11	1.6584725116961335E-4	4.87786032851804E-4	0.0	5.8534323942216475E-5	5.8534323942216475E-5
12-13	1.7560297182664942E-4	4.87786032851804E-4	0.0	5.8534323942216475E-5	5.8534323942216475E-5
14-15	1.951144131407216E-4	5.950989600792009E-4	0.0	5.8534323942216475E-5	7.804576525628864E-5
16-17	2.5364873708293807E-4	6.04854680736237E-4	0.0	5.8534323942216475E-5	7.804576525628864E-5
18-19	2.5364873708293807E-4	6.536332840214173E-4	0.0	5.8534323942216475E-5	7.804576525628864E-5
20-21	2.5364873708293807E-4	7.121676079636337E-4	0.0	6.829004459925255E-5	7.804576525628864E-5
22-23	2.5364873708293807E-4	7.804576525628863E-4	0.0	9.75572065703608E-5	1.0731292722739686E-4
24-25	2.5364873708293807E-4	8.975263004473192E-4	0.0	1.0731292722739686E-4	1.365800891985051E-4
26-27	2.5364873708293807E-4	9.560606243895357E-4	0.0	1.5609153051257725E-4	1.365800891985051E-4
28-29	2.5364873708293807E-4	9.75572065703608E-4	0.0	5.463203567940204E-4	1.365800891985051E-4
30-31	2.5364873708293807E-4	0.0011901979201584018	0.0	0.001824319762865747	1.365800891985051E-4
32-33	2.5364873708293807E-4	0.0011901979201584018	0.0	0.00415593699989737	1.365800891985051E-4
34-35	2.731601783970102E-4	0.001336533730013943	0.0	0.0075216606265748175	1.365800891985051E-4
36-37	2.731601783970102E-4	0.00149262526052652	0.0	0.012145872218009918	1.4633580985554118E-4
38-39	2.731601783970102E-4	0.0015511595844687367	0.0	0.019443151269472908	1.5609153051257728E-4
40-41	2.731601783970102E-4	0.0016584725116961334	0.0	0.026955056175390686	1.5609153051257728E-4
42-43	2.926716197110824E-4	0.0017365182769524222	0.0	0.03386210640057223	1.5609153051257728E-4
44-45	2.926716197110824E-4	0.001970655572721288	0.0	0.042027644590511434	1.5609153051257728E-4
46-47	2.926716197110824E-4	0.0021657699858620098	0.0	0.05107119763958387	1.5609153051257728E-4
48-49	2.926716197110824E-4	0.0021657699858620098	0.0	0.05972452186237488	1.5609153051257728E-4
50-51	2.926716197110824E-4	0.002565754532800489	0.0	0.06855344905699254	1.5609153051257728E-4
52-53	2.926716197110824E-4	0.0025950216947715973	0.0	0.07732384192766797	1.7560297182664944E-4
54-55	2.926716197110824E-4	0.0026047774154286333	0.0	0.08575278457534713	1.951144131407216E-4
56-57	2.926716197110824E-4	0.0026145331360856693	0.0	0.09343053673243454	1.951144131407216E-4
58-59	2.926716197110824E-4	0.0026145331360856693	0.0	0.1004253884435294	1.951144131407216E-4
60-61	3.1218306102515455E-4	0.002653556018713814	0.0	0.10725439290345465	2.1462585445479376E-4
62-63	3.316945023392267E-4	0.002673067460027886	0.0	0.11380048146432586	2.5364873708293807E-4
64-65	3.316945023392267E-4	0.002926716197110824	0.0	0.12044412723176744	2.5364873708293807E-4
66-67	3.316945023392267E-4	0.0029559833590819323	0.0	0.1271950859264364	2.5364873708293807E-4
68-69	3.316945023392267E-4	0.002995006241710076	0.0	0.13422896052015942	2.5364873708293807E-4
70-71	3.316945023392267E-4	0.0030242734036811845	0.0	0.14027750732752178	2.731601783970102E-4
72-73	3.902288262814432E-4	0.003053540565652293	0.0	0.14489196319829983	3.316945023392267E-4
74-75	3.902288262814432E-4	0.0031413420515656173	0.0	0.14873571713717207	3.316945023392267E-4
76-77	4.097402675955153E-4	0.0032193878168219063	0.0	0.1526087382380154	3.4145022299626276E-4
78-79	4.097402675955153E-4	0.0032584106994500506	0.0	0.15530131713935735	3.5120594365329884E-4
80-81	4.097402675955153E-4	0.0032584106994500506	0.0	0.15618908771914763	3.6096166431033496E-4
82-83	4.194959882525514E-4	0.0032779221407641225	0.0	0.15668662947265646	3.7071738496737103E-4
84-85	4.292517089095875E-4	0.003316945023392267	0.0	0.15695003393039644	3.7071738496737103E-4
86-87	4.292517089095875E-4	0.003316945023392267	0.0	0.15706710257828088	3.7071738496737103E-4
88	4.292517089095875E-4	0.0033754793473344835	0.0	0.15706710257828088	3.7071738496737103E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6015	0.0	50.00448	1
GTATCAA	10475	0.0	34.06321	1
TCAACGC	12855	0.0	26.82718	4
ATCAACG	13170	0.0	26.248098	3
CAACGCA	13285	0.0	25.958858	5
TATCAAC	13510	0.0	25.862005	2
AACGCAG	13535	0.0	25.601147	6
ACGCAGA	16135	0.0	21.424696	7
GTACATG	15815	0.0	21.206884	1
CGCAGAG	16475	0.0	20.982548	8
TACATGG	15875	0.0	20.86719	2
ACATGGG	16015	0.0	20.401773	3
GTGGTAT	3125	0.0	19.249725	1
TGGTATC	2995	0.0	19.122278	2
GAGTACT	10415	0.0	18.000141	12-13
GCAGAGT	19125	0.0	17.859724	9
CATGGGG	12405	0.0	17.570356	4
CAGAGTA	18505	0.0	17.09991	10-11
AGAGTAC	17055	0.0	16.669365	10-11
GTACTTT	11575	0.0	16.144815	14-15
>>END_MODULE