FastQCFastQC Report
Fri 27 May 2016
SRR2138706_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138706_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9364078
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104550.046.2141151
GTATCAA181750.035.142181
TCAACGC220750.028.4276334
ATCAACG223650.027.9850353
CAACGCA227600.027.517535
AACGCAG231000.027.2557756
TATCAAC233500.027.1411172
ACGCAGA278050.022.58427
GTACATG277950.022.3988461
CGCAGAG285300.021.9957948
TACATGG280900.021.7512682
ACATGGG283850.021.0297093
GCAGAGT334850.018.6915159
CATGGGG221350.018.6512034
GTGGTAT61800.018.5415971
GAGTACT188050.017.89544112-13
AGAGTAC294400.017.32540710-11
CAGAGTA323950.016.90067710-11
GTACTTT206550.016.30340214-15
GAGTACA201150.016.2260511
Produced by FastQC (version 0.11.5)