FastQCFastQC Report
Fri 27 May 2016
SRR2138705_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138705_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3312411
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35600.047.6781161
GTATCAA63850.034.564671
TCAACGC73500.028.5727864
ATCAACG75450.027.8343263
CAACGCA76450.027.5239965
TATCAAC79150.026.9485532
AACGCAG78500.026.857576
GTACATG99400.023.2777521
TACATGG99350.023.0412672
ACGCAGA95000.022.279367
CGCAGAG97900.021.7033588
ACATGGG102500.021.6515463
CATGGGG75950.020.237784
GCAGAGT115350.018.2775849
GAGTACT63450.017.81230512-13
GAGTACA70050.017.0140971
CAGAGTA110400.016.97519510-11
AGAGTAC100650.016.72088210-11
GTGGTAT19600.016.5649471
AGTACAT72550.016.0879232
Produced by FastQC (version 0.11.5)