##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2138705_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3312411 Sequences flagged as poor quality 0 Sequence length 19-100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49229579300395 34.0 31.0 34.0 31.0 34.0 2 32.70854854666284 34.0 31.0 34.0 31.0 34.0 3 32.73599109530792 34.0 31.0 34.0 31.0 34.0 4 35.521231211948034 37.0 34.0 37.0 34.0 37.0 5 35.33731381763918 37.0 34.0 37.0 34.0 37.0 6 36.25373089269417 37.0 36.0 37.0 35.0 38.0 7 36.217246289787106 37.0 36.0 37.0 35.0 38.0 8 36.19305424357062 37.0 36.0 37.0 35.0 38.0 9 37.55330241325729 39.0 37.0 39.0 35.0 39.0 10-11 37.49507896212155 39.0 37.0 39.0 35.0 39.0 12-13 37.43943686336026 39.0 37.0 39.0 35.0 39.0 14-15 38.45299903906852 39.0 38.0 41.0 35.0 41.0 16-17 38.39426825354704 39.0 38.0 41.0 35.0 41.0 18-19 38.42252652222203 39.0 38.0 41.0 35.0 41.0 20-21 38.42602668270809 39.0 38.0 41.0 35.0 41.0 22-23 38.36566036545992 39.0 38.0 41.0 35.0 41.0 24-25 38.30695187060793 39.0 38.0 41.0 34.5 41.0 26-27 38.16454096422488 39.0 38.0 41.0 34.0 41.0 28-29 38.04599068360565 39.0 38.0 41.0 34.0 41.0 30-31 37.96895518659821 39.0 38.0 41.0 34.0 41.0 32-33 37.83516537257782 38.0 37.5 41.0 34.0 41.0 34-35 37.80449322801957 38.0 37.5 41.0 34.0 41.0 36-37 37.79189922459591 38.0 37.0 41.0 34.0 41.0 38-39 37.775153843716694 38.0 37.0 41.0 34.0 41.0 40-41 37.78854960003163 38.5 37.0 41.0 34.0 41.0 42-43 37.836466247846985 39.0 37.5 41.0 34.0 41.0 44-45 37.787680673004374 39.0 37.0 41.0 33.5 41.0 46-47 37.70001522563791 38.0 37.0 41.0 33.0 41.0 48-49 37.63165627892582 38.0 37.0 41.0 33.0 41.0 50-51 37.5638133545191 38.0 37.0 41.0 33.0 41.0 52-53 37.438500275690636 38.0 37.0 40.5 33.0 41.0 54-55 37.3311145897646 38.0 37.0 40.0 33.0 41.0 56-57 37.20772149362052 38.0 36.5 40.0 32.5 41.0 58-59 37.04330289802383 38.0 36.0 40.0 32.0 41.0 60-61 36.88329221306868 38.0 36.0 40.0 32.0 41.0 62-63 36.72684107141393 38.0 35.0 40.0 32.0 41.0 64-65 36.48838393893806 38.0 35.0 39.5 31.0 41.0 66-67 36.23093022766314 38.0 35.0 39.0 31.0 41.0 68-69 36.013439479327786 37.5 35.0 39.0 31.0 41.0 70-71 35.73074798677628 37.0 35.0 38.5 31.0 40.0 72-73 35.49654177649546 37.0 34.5 38.0 30.0 40.0 74-75 35.26353929233349 36.0 34.0 38.0 30.0 39.5 76-77 34.23824187355102 35.0 32.5 38.0 28.5 38.5 78-79 34.789840810794935 35.5 34.0 38.0 30.0 39.0 80-81 34.74224506532103 35.0 34.0 38.0 30.0 38.0 82-83 34.56892371487707 35.0 34.0 37.5 30.0 38.0 84-85 34.41414362219983 35.0 34.0 37.0 30.0 38.0 86-87 34.24950377367719 35.0 34.0 37.0 30.0 38.0 88-89 34.18157305300937 35.0 34.0 37.0 30.0 38.0 90-91 34.093433305304984 35.0 34.0 36.5 30.0 38.0 92-93 33.98798396817696 35.0 34.0 36.0 30.0 38.0 94-95 33.97956284227406 35.0 34.0 36.0 30.0 38.0 96-97 34.02216267642172 35.0 34.0 36.0 30.0 38.0 98-99 34.068765588566926 35.0 34.0 36.0 30.0 38.0 100 33.1303095915568 34.0 32.0 35.0 27.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 3.0 15 5.0 16 8.0 17 19.0 18 26.0 19 55.0 20 109.0 21 277.0 22 861.0 23 2027.0 24 4522.0 25 8586.0 26 15092.0 27 22755.0 28 32659.0 29 43931.0 30 56990.0 31 74708.0 32 96256.0 33 125462.0 34 172111.0 35 243716.0 36 394787.0 37 996843.0 38 634847.0 39 368571.0 40 17185.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.55175459808581 19.80798880332181 13.386382305818934 24.25387429277345 2 15.652707348212525 20.76433147939673 39.947488400443056 23.635472771947686 3 18.960599998007492 25.013623007531372 29.829510891009598 26.19626610345153 4 13.063928359131763 16.927639716206716 37.69586563986172 32.31256628479981 5 13.752369497625747 37.44133804651657 35.097305255899705 13.708987199957976 6 31.68106252515162 36.64650914394379 17.95507864211295 13.71734968879164 7 27.862575024657264 32.0298718969355 22.182452600235898 17.925100478171338 8 25.46764275326945 36.15647333618926 20.478587953004624 17.89729595753667 9 25.883050140818874 16.38410813150904 20.837722130496488 36.895119597175594 10-11 25.090183555120426 26.314971179603013 28.153375290687055 20.441469974589506 12-13 25.51893469741527 23.711580477181123 28.53945358833792 22.23003123706569 14-15 22.589271077773862 25.198533636073545 25.982630174818283 26.229565111334313 16-17 22.38816559008347 28.367077913246508 26.705296161388308 22.539460335281714 18-19 22.368540618902667 27.614055139896585 28.310028556238947 21.7073756849618 20-21 23.1182355012788 26.70733045646606 28.236970054042775 21.93746398821236 22-23 22.8936504905228 26.65383967977319 28.045857107164718 22.40665272253929 24-25 22.598979332841406 26.764738167724435 28.186783992506026 22.449498506928133 26-27 22.57646919512883 26.914939442692738 28.117098807461776 22.391492554716656 28-29 22.552464040074167 27.027739002849227 28.055936251800794 22.363860705275815 30-31 22.82581482276445 26.882044488334504 27.948342866000058 22.343797822900985 32-33 22.554973202129258 27.01902337660761 27.93468939211106 22.491314029152075 34-35 22.69191320224856 27.068960893963695 27.949072200634784 22.290053703152957 36-37 22.732529579964655 27.049161991656845 27.840039739243394 22.378268689135112 38-39 22.557884541216733 27.147254022266477 27.93949768603933 22.35536375047746 40-41 22.807239802928216 26.97619908008858 27.8084575939908 22.40810352299241 42-43 22.6348937053404 27.08334938438643 27.648514170393785 22.633242739879385 44-45 22.71631655689362 27.14499097784066 27.5504877113392 22.588204753926522 46-47 22.81463815800049 27.06084593656344 27.373132341792783 22.751383563643284 48-49 22.729560478678312 27.251844056988794 27.304483775437046 22.714111688895848 50-51 22.70430020982368 27.329119320961176 27.279923140834093 22.68665732838105 52-53 22.854856346921842 27.21307678786753 27.168884530949033 22.763182334261593 54-55 22.79715244647404 27.125237682329466 27.223417023931777 22.854192847264716 56-57 22.80297799699088 27.23969878887631 27.189020105659022 22.768303108473788 58-59 22.780914444685 27.308187225109354 27.212765983058045 22.698132347147602 60-61 22.855362540064906 27.238008377187523 27.240619177371673 22.666009905375898 62-63 22.846691276764627 27.321470681420045 27.175560661672442 22.65627738014289 64-65 22.791736121814605 27.34837989463903 27.118681214903063 22.741202768643305 66-67 22.82711046900033 27.304172954135076 27.02072708098211 22.84798949588248 68-69 22.72351739662326 27.29646947384975 27.160163771648634 22.819849357878354 70-71 22.787339255220875 27.11428897044585 27.130040314174448 22.96833146015883 72-73 22.82876716186457 27.22978922329714 27.014656931675457 22.926786683162835 74-75 22.64064107011362 27.329672009931897 27.07848377241828 22.951203147536194 76-77 22.80967020601165 27.21829835897841 27.004704461000568 22.96732697400937 78-79 22.717249324772144 27.258343437089977 27.01962326319402 23.004783974943855 80-81 22.79180829745001 27.256368628992877 26.997786754773696 22.954036318783412 82-83 22.753011448681978 27.259141906759936 27.113422270295047 22.874424374263032 84-85 22.839665471339043 27.307610143814383 27.01815495555436 22.834569429292213 86-87 22.68097497142661 27.342733652991352 27.09396369943875 22.882327676143294 88-89 22.71463152091155 27.302790831998742 27.043062301451865 22.939515345637847 90-91 22.80453475574052 27.31372868086938 27.04683513974433 22.834901423645768 92-93 22.855844630522903 27.236052554150668 27.112059805097132 22.796043010229294 94-95 22.672329875649147 27.331921738414128 27.170471123125857 22.825277262810868 96-97 22.734864551956484 27.43961732330289 27.142056616943744 22.683461507796885 98-99 23.84767518054716 29.373260291448844 27.30582952334159 19.473235004662406 100 30.685740763832996 40.88002902066589 28.434230215501117 0.0 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 704.0 1 602.5 2 715.0 3 992.0 4 1266.0 5 1916.0 6 2554.5 7 3109.5 8 3545.0 9 3603.5 10 3302.5 11 2927.5 12 3103.5 13 3937.5 14 5855.0 15 8525.0 16 10870.5 17 12352.0 18 12919.0 19 12859.0 20 12453.0 21 12253.0 22 12664.0 23 14137.5 24 17042.0 25 21080.0 26 26189.0 27 32036.0 28 38450.0 29 45645.5 30 52589.0 31 59258.0 32 66404.5 33 73342.0 34 79737.0 35 86694.0 36 93995.0 37 100553.5 38 106592.0 39 111513.0 40 115976.0 41 120713.0 42 124857.0 43 128703.0 44 133334.0 45 137538.5 46 140227.0 47 142780.5 48 144675.0 49 145590.5 50 145710.0 51 144656.0 52 141389.5 53 136414.5 54 131231.5 55 124335.0 56 115870.0 57 106918.0 58 96531.0 59 83953.5 60 71157.5 61 59401.0 62 48357.0 63 37820.5 64 28687.0 65 22191.0 66 17181.0 67 13092.0 68 9966.5 69 7485.5 70 5348.0 71 3709.5 72 2607.5 73 1771.5 74 1180.0 75 794.0 76 512.5 77 322.0 78 208.5 79 133.5 80 88.0 81 56.0 82 37.5 83 25.0 84 17.5 85 12.5 86 9.0 87 5.5 88 3.0 89 1.5 90 1.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.547372593557985E-5 18-19 0.0 20-21 7.849508024536959E-4 22-23 4.528927391481238E-4 24-25 5.435636850537652E-4 26-27 0.0017671172110721824 28-29 0.0014811165200411692 30-31 0.001907203355709168 32-33 0.0010017795247193651 34-35 9.141397305237961E-4 36-37 0.00497941509798493 38-39 0.002393988031913251 40-41 6.233056798574251E-4 42-43 4.717041949282994E-5 44-45 5.242643022876194E-4 46-47 0.002330801118848835 48-49 2.1140404484237552E-4 50-51 0.0011180825016635588 52-53 0.0022095640231143657 54-55 0.0011585934809470982 56-57 1.6989137485274667E-4 58-59 0.0012209092575530434 60-61 2.784845774370751E-4 62-63 0.0011975573352588176 64-65 0.0026548905935622645 66-67 0.0031218313637224066 68-69 0.0016274497003755384 70-71 0.0011674389058836698 72-73 0.001370489751083391 74-75 0.018139772077756876 76-77 0.006319744790208558 78-79 0.002226781263862446 80-81 0.0047163997144794665 82-83 0.009367962092320261 84-85 0.004504698237195396 86-87 0.0063067373738083885 88-89 0.007581666731772393 90-91 2.77612930270679E-4 92-93 4.3460460976417486E-5 94-95 2.6572517285422493E-4 96-97 0.0030663712653301524 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 65.0 20-21 216.0 22-23 458.0 24-25 876.0 26-27 1718.0 28-29 3825.0 30-31 8549.0 32-33 11368.0 34-35 16114.0 36-37 24720.0 38-39 28677.0 40-41 28574.0 42-43 31085.0 44-45 36318.0 46-47 36405.0 48-49 34474.0 50-51 31682.0 52-53 31211.0 54-55 34207.0 56-57 35375.0 58-59 35127.0 60-61 34139.0 62-63 32659.0 64-65 34659.0 66-67 36430.0 68-69 36321.0 70-71 35478.0 72-73 33717.0 74-75 34378.0 76-77 34705.0 78-79 36572.0 80-81 36415.0 82-83 34381.0 84-85 34454.0 86-87 36704.0 88-89 38862.0 90-91 40382.0 92-93 41762.0 94-95 49279.0 96-97 115238.0 98-99 693424.0 100-101 1411408.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.22588617476931 #Duplication Level Percentage of deduplicated Percentage of total 1 92.3109877562242 82.36529686217385 2 5.718835041612659 10.205362489504266 3 1.1663914156537105 3.1221692306503805 4 0.3937290825054613 1.4052330519731462 5 0.17006857860609226 0.7587259818305999 6 0.08023100667005616 0.42952096012977525 7 0.04577252158613845 0.28588656606838725 8 0.029169187904190447 0.20821173117998043 9 0.018459449856029375 0.14823546945326452 >10 0.06422794447366532 0.879785552364061 >50 0.0013831979042757793 0.08492679144588455 >100 7.448170035891958E-4 0.10664531322646469 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 6.0378980748463883E-5 0.0 3.0189490374231942E-5 0.0 3 0.0 6.0378980748463883E-5 0.0 6.0378980748463883E-5 0.0 4 0.0 2.113264326196236E-4 0.0 6.0378980748463883E-5 0.0 5 0.0 2.113264326196236E-4 0.0 6.0378980748463883E-5 0.0 6 0.0 2.113264326196236E-4 0.0 6.0378980748463883E-5 0.0 7 0.0 2.113264326196236E-4 0.0 6.0378980748463883E-5 0.0 8 0.0 2.717054133680875E-4 0.0 6.0378980748463883E-5 3.0189490374231942E-5 9 0.0 4.528423556134791E-4 0.0 6.0378980748463883E-5 3.0189490374231942E-5 10-11 0.0 4.528423556134791E-4 0.0 1.509474518711597E-4 3.0189490374231942E-5 12-13 0.0 4.528423556134791E-4 0.0 1.509474518711597E-4 3.0189490374231942E-5 14-15 0.0 5.43410826736175E-4 0.0 1.6604219705827566E-4 3.0189490374231942E-5 16-17 0.0 5.43410826736175E-4 0.0 1.8113694224539164E-4 3.0189490374231942E-5 18-19 3.0189490374231942E-5 5.58505571923291E-4 0.0 1.8113694224539164E-4 3.0189490374231942E-5 20-21 3.0189490374231942E-5 5.736003171104069E-4 0.0 2.4151592299385553E-4 3.0189490374231942E-5 22-23 3.0189490374231942E-5 6.641687882331027E-4 0.0 2.4151592299385553E-4 4.5284235561347916E-5 24-25 3.0189490374231942E-5 8.000214949171465E-4 0.0 2.4151592299385553E-4 6.0378980748463883E-5 26-27 3.0189490374231942E-5 8.754952208527263E-4 0.0 3.018949037423194E-4 6.0378980748463883E-5 28-29 3.0189490374231942E-5 8.905899660398423E-4 0.0 6.641687882331027E-4 6.0378980748463883E-5 30-31 3.0189490374231942E-5 0.001056632163098118 0.0 0.002052885345447772 6.0378980748463883E-5 32-33 3.0189490374231942E-5 0.001056632163098118 0.0 0.00427181288795382 6.0378980748463883E-5 34-35 6.0378980748463883E-5 0.0011773901245950456 0.0 0.008151162401042624 6.0378980748463883E-5 36-37 6.0378980748463883E-5 0.0012981480860919734 0.0 0.013358849490597634 9.056847112269582E-5 38-39 6.0378980748463883E-5 0.0012981480860919734 0.0 0.021253401223459287 9.056847112269582E-5 40-41 6.0378980748463883E-5 0.0015094745187115971 0.0 0.02893662652370132 9.056847112269582E-5 42-43 6.0378980748463883E-5 0.0015245692638987131 0.0 0.03637833590094949 9.056847112269582E-5 44-45 6.0378980748463883E-5 0.0016755167157698728 0.0 0.0454653725035933 9.056847112269582E-5 46-47 6.0378980748463883E-5 0.0018113694224539164 0.0 0.05495996722628925 9.056847112269582E-5 48-49 6.0378980748463883E-5 0.0018113694224539164 0.0 0.0642734250067398 9.056847112269582E-5 50-51 6.0378980748463883E-5 0.0019925063646993083 0.0 0.07408500937836518 9.056847112269582E-5 52-53 6.0378980748463883E-5 0.0019925063646993083 0.0 0.08367017257218382 1.0566321630981179E-4 54-55 6.0378980748463883E-5 0.0019925063646993083 0.0 0.09314967254969267 1.2075796149692777E-4 56-57 6.0378980748463883E-5 0.0019925063646993083 0.0 0.1018895300130328 1.2075796149692777E-4 58-59 6.0378980748463883E-5 0.002007601109886424 0.0 0.1096331342940233 1.2075796149692777E-4 60-61 6.0378980748463883E-5 0.0020377906002606557 0.0 0.11733145433945244 1.2075796149692777E-4 62-63 9.056847112269582E-5 0.0020981695810091197 0.0 0.12425994238033866 1.509474518711597E-4 64-65 9.056847112269582E-5 0.0024755382106870193 0.0 0.13124880940197337 1.509474518711597E-4 66-67 9.056847112269582E-5 0.0024755382106870193 0.0 0.13905279266371232 1.509474518711597E-4 68-69 9.056847112269582E-5 0.002551011936622599 0.0 0.14660016525727032 1.509474518711597E-4 70-71 1.0566321630981179E-4 0.002581201426996831 0.0 0.15363431651446635 1.509474518711597E-4 72-73 1.509474518711597E-4 0.0026113909173710627 0.0 0.15940050917594467 1.8113694224539164E-4 74-75 1.509474518711597E-4 0.002626485662558179 0.0 0.163219479708285 1.8113694224539164E-4 76-77 1.509474518711597E-4 0.002641580407745295 0.0 0.1671743029473094 1.8113694224539164E-4 78-79 1.509474518711597E-4 0.0026717698981195267 0.0 0.17008758876842275 1.8113694224539164E-4 80-81 1.509474518711597E-4 0.002686864643306643 0.0 0.1709630839892755 1.8113694224539164E-4 82-83 1.509474518711597E-4 0.0027321488788679908 0.0 0.17168763175825705 1.8113694224539164E-4 84-85 1.509474518711597E-4 0.0027774331144293386 0.0 0.17203481089756073 1.8113694224539164E-4 86-87 1.509474518711597E-4 0.002852906840364919 0.0 0.17214047411387054 1.8113694224539164E-4 88 1.509474518711597E-4 0.0030189490374231943 0.0 0.17214047411387054 1.8113694224539164E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3560 0.0 47.678116 1 GTATCAA 6385 0.0 34.56467 1 TCAACGC 7350 0.0 28.572786 4 ATCAACG 7545 0.0 27.834326 3 CAACGCA 7645 0.0 27.523996 5 TATCAAC 7915 0.0 26.948553 2 AACGCAG 7850 0.0 26.85757 6 GTACATG 9940 0.0 23.277752 1 TACATGG 9935 0.0 23.041267 2 ACGCAGA 9500 0.0 22.27936 7 CGCAGAG 9790 0.0 21.703358 8 ACATGGG 10250 0.0 21.651546 3 CATGGGG 7595 0.0 20.23778 4 GCAGAGT 11535 0.0 18.277584 9 GAGTACT 6345 0.0 17.812305 12-13 GAGTACA 7005 0.0 17.014097 1 CAGAGTA 11040 0.0 16.975195 10-11 AGAGTAC 10065 0.0 16.720882 10-11 GTGGTAT 1960 0.0 16.564947 1 AGTACAT 7255 0.0 16.087923 2 >>END_MODULE