FastQCFastQC Report
Fri 27 May 2016
SRR2138704_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138704_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3872857
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46000.051.1173021
GTATCAA79500.032.8982661
TCAACGC94700.026.7902074
ATCAACG95350.026.4777853
TATCAAC99250.025.769862
CAACGCA98750.025.6079165
AACGCAG102300.024.840256
GTACATG126600.022.679131
TACATGG123150.022.4770342
ACGCAGA118050.021.5960037
ACATGGG124850.021.3118953
TGGTATC20700.020.3273492
CGCAGAG132200.019.284488
GTGGTAT24150.018.448351
GAGTACT76600.018.44541212-13
CATGGGG93500.018.309964
GCAGAGT140100.018.079289
CAGAGTA136300.017.81177110-11
GAGTACA88700.017.3009591
ATGGGGG52400.016.7686945
Produced by FastQC (version 0.11.5)