##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138704_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3872857
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22137533092495	33.0	31.0	34.0	31.0	34.0
2	32.479595812600365	34.0	31.0	34.0	31.0	34.0
3	32.497978365842066	34.0	31.0	34.0	31.0	34.0
4	36.06378934208002	37.0	35.0	37.0	35.0	37.0
5	35.82579656310574	37.0	35.0	37.0	34.0	37.0
6	35.97888458055642	37.0	35.0	37.0	35.0	37.0
7	35.93877956247804	37.0	35.0	37.0	35.0	37.0
8	35.95028244006944	37.0	35.0	37.0	35.0	37.0
9	37.715977636148196	39.0	37.0	39.0	35.0	39.0
10-11	37.6313931807965	39.0	37.0	39.0	35.0	39.0
12-13	37.59076516380543	39.0	37.0	39.0	35.0	39.0
14-15	38.87632979993839	40.0	38.0	41.0	35.0	41.0
16-17	38.834857186826156	40.0	38.0	41.0	35.0	41.0
18-19	38.84106035931613	40.0	38.0	41.0	35.0	41.0
20-21	38.85454492672144	40.0	38.0	41.0	35.0	41.0
22-23	38.77500033740882	40.0	38.0	41.0	35.0	41.0
24-25	38.70454784186582	40.0	38.0	41.0	34.0	41.0
26-27	38.55236675940262	40.0	38.0	41.0	34.0	41.0
28-29	38.42448893290472	40.0	38.0	41.0	34.0	41.0
30-31	38.299568437740334	40.0	38.0	41.0	34.0	41.0
32-33	38.14570136791029	40.0	38.0	41.0	34.0	41.0
34-35	38.10415229077733	40.0	37.5	41.0	33.5	41.0
36-37	38.11218327889277	40.0	38.0	41.0	33.5	41.0
38-39	38.06954008098225	40.0	37.0	41.0	33.0	41.0
40-41	38.09895024986811	40.0	37.5	41.0	33.0	41.0
42-43	38.15412864148644	40.0	38.0	41.0	33.0	41.0
44-45	38.131408963428974	40.0	37.5	41.0	33.0	41.0
46-47	38.03174678531319	40.0	37.0	41.0	33.0	41.0
48-49	37.94297922885149	40.0	37.0	41.0	33.0	41.0
50-51	37.84508374533908	40.0	37.0	41.0	33.0	41.0
52-53	37.686186534863495	40.0	36.5	41.0	32.5	41.0
54-55	37.55890247227046	39.0	36.0	41.0	32.0	41.0
56-57	37.40101907397728	39.0	36.0	41.0	32.0	41.0
58-59	37.17172082500335	39.0	35.0	41.0	31.5	41.0
60-61	36.98026237551639	39.0	35.0	40.5	31.0	41.0
62-63	36.75690633416785	38.0	35.0	40.0	31.0	41.0
64-65	36.45220899498332	38.0	35.0	40.0	31.0	41.0
66-67	36.123957200351086	37.0	35.0	40.0	31.0	41.0
68-69	35.87205789350082	37.0	34.5	39.5	30.0	41.0
70-71	35.495559594727524	36.0	34.0	39.0	30.0	41.0
72-73	35.21452779274003	36.0	34.0	39.0	30.0	40.0
74-75	34.90042767617415	35.0	34.0	38.0	29.5	40.0
76-77	33.559514633371066	34.5	32.0	36.5	28.0	39.0
78-79	34.30666568370014	35.0	33.5	37.0	29.0	39.0
80-81	34.26284361454084	35.0	34.0	36.5	30.0	39.0
82-83	34.03069564151279	35.0	34.0	36.0	29.5	38.0
84-85	33.83764478002166	35.0	33.5	36.0	29.0	37.0
86-87	33.6202686253841	35.0	33.0	35.0	29.0	37.0
88-89	33.52248404006998	35.0	33.0	35.0	29.0	36.5
90-91	33.426536731049666	35.0	33.0	35.0	29.0	36.0
92-93	33.266657434476244	35.0	33.0	35.0	29.0	36.0
94-95	33.28702281779654	35.0	33.0	35.0	29.0	36.0
96-97	33.34518212707196	35.0	33.0	35.0	29.0	35.5
98-99	33.43387202252981	35.0	33.0	35.0	30.0	35.0
100	32.27263520356492	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	3.0
19	4.0
20	34.0
21	130.0
22	527.0
23	1814.0
24	4514.0
25	10011.0
26	18368.0
27	29846.0
28	43642.0
29	59046.0
30	76011.0
31	95265.0
32	118896.0
33	151296.0
34	204847.0
35	287500.0
36	458860.0
37	815658.0
38	954346.0
39	519129.0
40	23106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.60505874603684	19.695795636141483	13.575817542449927	24.12332807537175
2	15.600756753993242	20.793486565602603	40.15598820199145	23.449768478412707
3	19.25433859293023	24.751081694986414	29.65273440253539	26.341845309547963
4	13.050391481017758	17.120280970869825	37.350514103670754	32.47881344444166
5	13.92845126995394	37.228950100662125	34.949211912549316	13.893386716834627
6	31.816253479020784	36.597142626231744	17.785448830153037	13.801155064594434
7	27.88179372489095	31.838536770141523	22.015659240710413	18.26401026425711
8	25.363291234352314	36.30634955021577	20.252129112952012	18.0782301024799
9	25.688296779354364	16.36515368370172	20.899274101780673	37.04727543516324
10-11	25.14047123351056	26.462828346102114	27.780331161207343	20.616369259179983
12-13	25.425390093153453	23.91403813773656	28.194302035938847	22.466269733171146
14-15	22.435465600718025	25.278844532602157	25.939764881584836	26.34592498509498
16-17	22.24953341878285	28.513922039881724	26.597711453987916	22.638833087347514
18-19	22.288958771263694	27.6678044141573	28.179842426405106	21.863394388173898
20-21	23.08466471658786	26.73971973814408	28.285529031178534	21.89008651408953
22-23	22.830495890711653	26.701322848676185	28.044262813684284	22.42391844692788
24-25	22.57650153707586	26.820582183868186	28.294749196979087	22.308167082076867
26-27	22.48252833819499	26.943407849718447	28.216189551719488	22.35787426036707
28-29	22.496950918745426	26.99593770448691	28.185043837856238	22.322067538911423
30-31	22.757978269802397	26.762222831457777	28.10730960500427	22.372489293735555
32-33	22.58234517050841	26.860314892648816	28.01091615381938	22.546423783023393
34-35	22.719022755864966	27.003839359628714	28.023583706513875	22.253554177992452
36-37	22.889127881290396	26.857335742957243	27.80229263974072	22.451243736011634
38-39	22.480056757291592	27.194021078824772	27.93620436009049	22.389717803793154
40-41	22.742249421383654	26.99532626280784	27.826752211383926	22.43567210442458
42-43	22.573527304297794	27.13625768159817	27.641400680530154	22.64881433357388
44-45	22.50904524013999	27.178055067680358	27.62906253846055	22.683837153719104
46-47	22.67276321905785	27.14521703138294	27.44044594873054	22.741573800828675
48-49	22.744201464380076	27.407334156936862	27.195379277782344	22.653085100900718
50-51	22.684596249257567	27.402200676633008	27.20913243346531	22.70407064064411
52-53	22.951396361878686	27.295838787973793	27.10536793520634	22.64739691494118
54-55	22.855616674707147	27.29146161284718	27.10810415807111	22.74481755437456
56-57	22.89987352102132	27.514612507004006	26.896850464182503	22.688663507792175
58-59	22.767090754777257	27.48387341485191	27.04229526547247	22.706740564898357
60-61	22.831698928939918	27.475244506322234	26.98597904992084	22.70707751481701
62-63	22.801596430242192	27.47267817699227	27.06033770686008	22.665387685905465
64-65	22.88344474338283	27.343055065181037	27.060751396693068	22.712748794743064
66-67	22.891773559353336	27.45656707115321	26.809378342400297	22.84228102709316
68-69	22.86113748123012	27.39018724065776	26.96573394361026	22.78294133450186
70-71	22.894461402965565	27.271744033680346	26.93068065677555	22.90311390657854
72-73	22.815537550872005	27.368364334865795	26.89393200522766	22.922166109034535
74-75	22.692438184874298	27.409605009958277	26.936813506996693	22.961143298170732
76-77	22.784105202692018	27.459997099190474	26.814837394876385	22.941060303241123
78-79	22.64543968647585	27.46913713461	26.947897261345048	22.937525917569097
80-81	22.738138996939576	27.392615796000307	26.946309244426132	22.922935962633986
82-83	22.923372795809847	27.325250364051072	27.040461042609508	22.710915797529573
84-85	22.862645510626255	27.33108036229048	26.989346504051902	22.81692762303137
86-87	22.685957619414044	27.540556159662476	26.961490727689768	22.81199549323371
88-89	22.89030593839546	27.333044143596062	26.90936039484197	22.867289523166505
90-91	22.800830416635574	27.41170031102588	26.94824059912872	22.839228673209828
92-93	22.84930752131129	27.439901346310148	27.03078650061142	22.680004631767137
94-95	22.559067414312693	27.613368651027425	27.080857951454657	22.746705983205228
96-97	22.541727193365848	27.73875343403276	27.156788979870548	22.562730392730845
98-99	23.764479623153843	29.682544389351218	27.286546685333878	19.26642930216106
100	22.61881934598159	30.866338082056128	21.030270294600857	25.484572277361423
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1306.0
1	1059.0
2	1105.5
3	1472.0
4	1923.0
5	2946.0
6	3884.0
7	4676.0
8	5274.5
9	5376.0
10	5000.0
11	4532.0
12	4792.0
13	6147.0
14	9563.5
15	14215.0
16	17932.5
17	20046.0
18	20633.5
19	19990.5
20	18655.0
21	17178.5
22	16436.5
23	17313.0
24	20053.0
25	24539.5
26	30388.0
27	37077.5
28	43976.0
29	51429.5
30	58648.0
31	65752.0
32	73122.5
33	80278.0
34	88065.0
35	96052.0
36	104774.5
37	112140.0
38	117760.0
39	124255.0
40	130802.0
41	136524.5
42	141320.0
43	146615.5
44	151181.0
45	156680.0
46	164093.0
47	171131.0
48	174197.0
49	173694.0
50	173225.5
51	171266.0
52	163592.0
53	153710.0
54	145930.0
55	138040.0
56	130188.0
57	120178.5
58	108707.0
59	96125.5
60	82667.0
61	71614.5
62	60381.0
63	50339.5
64	42078.5
65	33348.5
66	26597.5
67	20009.5
68	13482.0
69	9744.0
70	7252.5
71	4951.0
72	3564.0
73	2543.0
74	1859.0
75	1323.0
76	837.5
77	474.5
78	334.0
79	246.5
80	166.5
81	103.5
82	70.0
83	44.0
84	26.5
85	20.5
86	18.5
87	16.0
88	12.0
89	8.5
90	6.0
91	4.0
92	3.5
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.2910365655122304E-4
18-19	0.0
20-21	0.0011361558888509024
22-23	4.390108105120881E-4
24-25	7.878073255749378E-4
26-27	0.0024547775073646555
28-29	0.002081593823381031
30-31	0.0024863789813952806
32-33	0.001220740854638075
34-35	0.0011472318208733093
36-37	0.006766282251792507
38-39	0.0030667243880596025
40-41	8.001083880162966E-4
42-43	5.382447810776817E-5
44-45	7.751545821427231E-4
46-47	0.003179583714034572
48-49	1.532067353580671E-4
50-51	0.00140810680884415
52-53	0.002873338815436202
54-55	0.001379580909186931
56-57	1.3078002291556622E-4
58-59	0.0019552785169285954
60-61	2.5281129882102194E-4
62-63	0.0012931994400747168
64-65	0.0032082388790808684
66-67	0.004416582528461181
68-69	0.0020569308596973695
70-71	0.0012947009311899613
72-73	0.0013590199835094917
74-75	0.022899437806664338
76-77	0.007767666155541124
78-79	0.002423617857216372
80-81	0.006210004165246696
82-83	0.012662526600691832
84-85	0.0055349656754292665
86-87	0.008508236060220067
88-89	0.008692750976019587
90-91	3.4413413406705147E-4
92-93	3.687711328907342E-5
94-95	3.7606546397170635E-4
96-97	0.0036710776274482223
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	99.0
20-21	281.0
22-23	630.0
24-25	1415.0
26-27	2273.0
28-29	4730.0
30-31	10303.0
32-33	13410.0
34-35	19961.0
36-37	28843.0
38-39	33117.0
40-41	33369.0
42-43	37091.0
44-45	43779.0
46-47	43529.0
48-49	39923.0
50-51	36349.0
52-53	35112.0
54-55	37721.0
56-57	40126.0
58-59	39167.0
60-61	37653.0
62-63	36129.0
64-65	38493.0
66-67	40286.0
68-69	42042.0
70-71	40358.0
72-73	37631.0
74-75	38616.0
76-77	38234.0
78-79	40707.0
80-81	41623.0
82-83	39198.0
84-85	39809.0
86-87	42874.0
88-89	45269.0
90-91	48715.0
92-93	50529.0
94-95	62281.0
96-97	141175.0
98-99	248357.0
100-101	2221650.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.25072301462258
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.36336363245972	84.2611107090661
2	4.963587430529972	8.959347087032455
3	0.9716296361839141	2.6307083150409882
4	0.30708471612281624	1.108584706272972
5	0.14344885806411112	0.6473181577954001
6	0.07705050963388177	0.4172318521861783
7	0.045742844053097025	0.2889827323975995
8	0.029072127119022878	0.20990243936518646
9	0.02334250692086588	0.18960103139237794
>10	0.07305160703325027	1.0174454225408274
>50	0.0018322959275589353	0.11115539188994045
>100	7.542760639975893E-4	0.13922538000772372
>500	3.9559887770994506E-5	0.01938677501224302
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.5820731310244607E-5	0.0	2.5820731310244607E-5	0.0
3	0.0	2.5820731310244607E-5	0.0	5.1641462620489215E-5	0.0
4	0.0	5.1641462620489215E-5	0.0	1.0328292524097843E-4	0.0
5	0.0	5.1641462620489215E-5	0.0	1.0328292524097843E-4	0.0
6	0.0	5.1641462620489215E-5	0.0	1.0328292524097843E-4	0.0
7	0.0	5.1641462620489215E-5	0.0	1.0328292524097843E-4	0.0
8	0.0	5.1641462620489215E-5	0.0	1.0328292524097843E-4	0.0
9	5.1641462620489215E-5	2.0656585048195686E-4	0.0	1.2910365655122304E-4	0.0
10-11	5.1641462620489215E-5	2.0656585048195686E-4	0.0	1.2910365655122304E-4	0.0
12-13	7.746219393073382E-5	2.0656585048195686E-4	0.0	1.4201402220634536E-4	0.0
14-15	1.0328292524097843E-4	3.4857987268830217E-4	0.0	1.5492438786146766E-4	0.0
16-17	2.0656585048195686E-4	3.614902383434245E-4	0.0	1.5492438786146766E-4	0.0
18-19	2.0656585048195686E-4	4.131317009639137E-4	0.0	1.5492438786146766E-4	0.0
20-21	2.0656585048195686E-4	4.6477316358440294E-4	0.0	1.5492438786146766E-4	0.0
22-23	2.0656585048195686E-4	5.164146262048922E-4	0.0	1.5492438786146766E-4	0.0
24-25	2.0656585048195686E-4	6.067871857907484E-4	0.0	1.8074511917171224E-4	2.5820731310244607E-5
26-27	2.0656585048195686E-4	6.971597453766044E-4	0.0	2.3238658179220147E-4	2.5820731310244607E-5
28-29	2.0656585048195686E-4	6.971597453766044E-4	0.0	7.100701110317267E-4	3.873109696536691E-5
30-31	2.3238658179220147E-4	8.004426706175828E-4	0.0	0.0021431206987503025	5.1641462620489215E-5
32-33	2.4529694744732377E-4	8.004426706175828E-4	0.0	0.004673552367154274	5.1641462620489215E-5
34-35	2.8402804441269073E-4	9.940981554444174E-4	0.0	0.008288454750588519	5.1641462620489215E-5
36-37	3.098487757229353E-4	0.0012135743715814965	0.0	0.01383991198229111	5.1641462620489215E-5
38-39	3.098487757229353E-4	0.0013168572968224749	0.0	0.021779786860191327	5.1641462620489215E-5
40-41	3.098487757229353E-4	0.0014201402220634534	0.0	0.030287717826916923	5.1641462620489215E-5
42-43	3.098487757229353E-4	0.0014846920503390649	0.0	0.03817595124219665	5.1641462620489215E-5
44-45	3.098487757229353E-4	0.0016396164382005327	0.0	0.047329400491678365	5.1641462620489215E-5
46-47	3.356695070331799E-4	0.0017299889977863887	0.0	0.05746403753094938	5.1641462620489215E-5
48-49	3.356695070331799E-4	0.0017428993634415108	0.0	0.06775359895808185	7.746219393073383E-5
50-51	3.356695070331799E-4	0.0019365548482683456	0.0	0.07770749087818114	7.746219393073383E-5
52-53	3.356695070331799E-4	0.00196237557957859	0.0	0.0867060157398014	7.746219393073383E-5
54-55	3.356695070331799E-4	0.0019752859452337127	0.0	0.09523976743783724	7.746219393073383E-5
56-57	3.4857987268830217E-4	0.002039837773509324	0.0	0.10296016609960038	7.746219393073383E-5
58-59	3.8731096965366913E-4	0.002039837773509324	0.0	0.11080966841791473	7.746219393073383E-5
60-61	4.0022133530879145E-4	0.0020527481391644465	0.0	0.11809111464740371	7.746219393073383E-5
62-63	4.131317009639137E-4	0.0020656585048195687	0.0	0.12494651881027366	1.0328292524097843E-4
64-65	4.26042066619036E-4	0.002246403623991281	0.0	0.1317890126074885	1.0328292524097843E-4
66-67	4.389524322741583E-4	0.00229804508661177	0.0	0.13917374176221842	1.0328292524097843E-4
68-69	4.389524322741583E-4	0.00229804508661177	0.0	0.1458613111715718	1.0328292524097843E-4
70-71	4.389524322741583E-4	0.00229804508661177	0.0	0.15199373485775486	1.0328292524097843E-4
72-73	4.389524322741583E-4	0.00229804508661177	0.0	0.15724825367938966	1.0328292524097843E-4
74-75	4.389524322741583E-4	0.0023109554522668923	0.0	0.16165068836778634	1.0328292524097843E-4
76-77	4.389524322741583E-4	0.002388417646197626	0.0	0.16547215660170256	1.0328292524097843E-4
78-79	4.389524322741583E-4	0.0024013280118527487	0.0	0.16813169192665778	1.0328292524097843E-4
80-81	4.389524322741583E-4	0.002414238377507871	0.0	0.16886758276899974	1.2910365655122304E-4
82-83	4.389524322741583E-4	0.002427148743162993	0.0	0.16939690776085975	1.5492438786146766E-4
84-85	4.389524322741583E-4	0.002427148743162993	0.0	0.16957765288003146	1.5492438786146766E-4
86-87	4.389524322741583E-4	0.0024529694744732378	0.0	0.16966802543961731	1.5492438786146766E-4
88	4.389524322741583E-4	0.0025304316684039716	0.0	0.16966802543961731	1.5492438786146766E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4600	0.0	51.117302	1
GTATCAA	7950	0.0	32.898266	1
TCAACGC	9470	0.0	26.790207	4
ATCAACG	9535	0.0	26.477785	3
TATCAAC	9925	0.0	25.76986	2
CAACGCA	9875	0.0	25.607916	5
AACGCAG	10230	0.0	24.84025	6
GTACATG	12660	0.0	22.67913	1
TACATGG	12315	0.0	22.477034	2
ACGCAGA	11805	0.0	21.596003	7
ACATGGG	12485	0.0	21.311895	3
TGGTATC	2070	0.0	20.327349	2
CGCAGAG	13220	0.0	19.28448	8
GTGGTAT	2415	0.0	18.44835	1
GAGTACT	7660	0.0	18.445412	12-13
CATGGGG	9350	0.0	18.30996	4
GCAGAGT	14010	0.0	18.07928	9
CAGAGTA	13630	0.0	17.811771	10-11
GAGTACA	8870	0.0	17.300959	1
ATGGGGG	5240	0.0	16.768694	5
>>END_MODULE