FastQCFastQC Report
Fri 27 May 2016
SRR2138703_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138703_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6961092
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78300.044.8226741
GTATCAA139750.035.170771
TCAACGC165250.028.9930444
ATCAACG167150.028.688213
CAACGCA171450.027.9928115
TATCAAC174100.027.8041732
AACGCAG175100.027.645386
ACGCAGA206250.023.4099627
CGCAGAG216150.022.3186268
TGGTATC41000.022.1814862
GTGGTAT43950.021.8213161
GTACATG199400.021.0422761
TACATGG199000.020.4613842
ACATGGG203700.019.360173
GCAGAGT250750.019.222489
GAGTACT138050.018.23626312-13
CAGAGTA240000.017.25883310-11
AGAGTAC222500.017.21354710-11
CATGGGG160750.017.152474
GTACTTT157250.016.128414-15
Produced by FastQC (version 0.11.5)