##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138703_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6961092
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.171998301415925	33.0	31.0	34.0	30.0	34.0
2	32.43854843464215	34.0	31.0	34.0	31.0	34.0
3	32.45951310512776	34.0	31.0	34.0	31.0	34.0
4	36.087282282722306	37.0	35.0	37.0	35.0	37.0
5	35.843887568214875	37.0	35.0	37.0	35.0	37.0
6	35.93368626646509	37.0	35.0	37.0	35.0	37.0
7	35.892719992782744	37.0	35.0	37.0	35.0	37.0
8	35.90541369084046	37.0	35.0	37.0	35.0	37.0
9	37.69632609366462	39.0	37.0	39.0	35.0	39.0
10-11	37.60767771780634	39.0	37.0	39.0	35.0	39.0
12-13	37.567512827010475	39.0	37.0	39.0	35.0	39.0
14-15	38.86609845122001	40.0	38.0	41.0	35.0	41.0
16-17	38.82203431588032	40.0	38.0	41.0	35.0	41.0
18-19	38.82458104274444	40.0	38.0	41.0	35.0	41.0
20-21	38.84210155370003	40.0	38.0	41.0	35.0	41.0
22-23	38.767547049608396	40.0	38.0	41.0	34.0	41.0
24-25	38.6949735119029	40.0	38.0	41.0	34.0	41.0
26-27	38.540241550912825	40.0	38.0	41.0	34.0	41.0
28-29	38.41313302546466	40.0	38.0	41.0	34.0	41.0
30-31	38.27946832463434	40.0	38.0	41.0	34.0	41.0
32-33	38.128650171362615	40.0	38.0	41.0	33.5	41.0
34-35	38.085373943506376	40.0	37.5	41.0	33.0	41.0
36-37	38.100182191127516	40.0	38.0	41.0	33.0	41.0
38-39	38.06319793600474	40.0	37.0	41.0	33.0	41.0
40-41	38.09730197671526	40.0	37.5	41.0	33.0	41.0
42-43	38.1528397118297	40.0	38.0	41.0	33.0	41.0
44-45	38.13541270085486	40.0	37.5	41.0	33.0	41.0
46-47	38.04089451535569	40.0	37.0	41.0	33.0	41.0
48-49	37.95727289259717	40.0	37.0	41.0	33.0	41.0
50-51	37.86172421881382	40.0	37.0	41.0	33.0	41.0
52-53	37.69644928199551	40.0	36.5	41.0	32.5	41.0
54-55	37.57006905898626	39.5	36.0	41.0	32.0	41.0
56-57	37.40817056405477	39.0	36.0	41.0	32.0	41.0
58-59	37.17889361025809	39.0	35.0	41.0	31.5	41.0
60-61	36.98700834650742	39.0	35.0	40.5	31.0	41.0
62-63	36.75820693032436	38.0	35.0	40.0	31.0	41.0
64-65	36.452115265173646	38.0	35.0	40.0	31.0	41.0
66-67	36.119299008118674	37.0	35.0	40.0	30.5	41.0
68-69	35.860293358085556	37.0	34.0	39.5	30.0	41.0
70-71	35.469752437755886	36.0	34.0	39.0	30.0	41.0
72-73	35.17855770406428	36.0	34.0	39.0	30.0	40.0
74-75	34.85637142280079	35.0	34.0	38.0	29.0	40.0
76-77	33.49263557515222	34.5	32.0	36.5	28.0	39.0
78-79	34.249733366866835	35.0	33.5	37.0	29.0	39.0
80-81	34.207116137118675	35.0	34.0	36.5	29.0	39.0
82-83	33.97142200675003	35.0	34.0	36.0	29.0	37.5
84-85	33.77663641513864	35.0	33.0	36.0	29.0	37.0
86-87	33.55419767179177	35.0	33.0	35.0	29.0	37.0
88-89	33.44848928276807	35.0	33.0	35.0	29.0	36.0
90-91	33.35296518731422	35.0	33.0	35.0	29.0	36.0
92-93	33.18973065180825	35.0	33.0	35.0	29.0	36.0
94-95	33.21207904639033	35.0	33.0	35.0	29.0	35.5
96-97	33.26531203293161	35.0	33.0	35.0	29.0	35.0
98-99	33.35792580999554	35.0	33.0	35.0	29.5	35.0
100	32.18978916358929	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	6.0
20	30.0
21	188.0
22	848.0
23	3131.0
24	8424.0
25	18698.0
26	34256.0
27	55550.0
28	80995.0
29	108993.0
30	139836.0
31	174916.0
32	218139.0
33	278167.0
34	371605.0
35	520358.0
36	815910.0
37	1419563.0
38	1737579.0
39	932950.0
40	40950.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.301021161622344	19.844214097443334	13.706125418253343	24.14863932268098
2	15.545075399089683	20.70968175682781	40.27847929606447	23.46676354801804
3	19.07644662647757	24.877404292315056	29.879665431802945	26.16648364940443
4	13.114149331742778	17.071832982526306	37.6242405645551	32.189777121175815
5	13.805793688691374	37.38985205194817	35.062975751505654	13.741378507854801
6	31.7198221198628	36.71208482807008	17.873919781551514	13.694173270515602
7	27.819615083380594	31.96787228210746	22.103213116562745	18.109299517949196
8	25.209923960206243	36.380254707163765	20.40599377224148	18.003827560388512
9	25.718766538353467	16.53118504970197	20.878362188001535	36.87168622394302
10-11	25.024277800092282	26.372880864094313	28.09095182192679	20.511889513886615
12-13	25.42249118385449	23.776693369373657	28.497066264890623	22.303749181881233
14-15	22.430352881415732	25.30658982814765	26.052392354532884	26.210664935903733
16-17	22.29119299143337	28.466922019726532	26.762962534123652	22.478922454716443
18-19	22.30887050480011	27.642660088388432	28.374937437976683	21.673531968834777
20-21	23.00454264237536	26.79353061526618	28.37465260002185	21.827274142336606
22-23	22.746008702434178	26.69363318329772	28.186168674009853	22.374189440258245
24-25	22.508504670976688	26.834473301016565	28.29880857725509	22.358213450751656
26-27	22.489505276095173	26.98127513977212	28.21323493525627	22.315984648876437
28-29	22.446822765278778	27.080103515286314	28.201823593478746	22.27125012595616
30-31	22.66611646074881	26.94623398623107	28.0716375019835	22.316012051036616
32-33	22.391482688413692	27.112408149298506	28.078164429682644	22.41794473260516
34-35	22.580010312056036	27.143388682529373	28.043964761560343	22.232636243854248
36-37	22.66338820636224	27.024134001416193	27.944814285573717	22.367663506647848
38-39	22.39497252361477	27.233480825872043	28.040013936487924	22.331532714025265
40-41	22.72597622959204	27.086439644928912	27.871246623222806	22.316337502256246
42-43	22.52142339545519	27.16378050021572	27.739379909209006	22.575416195120084
44-45	22.625877937277686	27.2859206942574	27.570952127884436	22.51724924058048
46-47	22.71358299315119	27.23039322211886	27.372536055309897	22.68348772942005
48-49	22.599347610136565	27.394680415958412	27.32086125598105	22.685110717923973
50-51	22.6302649511554	27.473928270682972	27.276566655904706	22.61924012225692
52-53	22.78915040651753	27.393767893024375	27.150809936082286	22.666271764375807
54-55	22.72536242424088	27.24089516481447	27.258220411227267	22.77552199971738
56-57	22.778928993391403	27.443796397432003	27.131772270465365	22.64550233871123
58-59	22.708378762613524	27.46808573550877	27.17673012958922	22.64680537228849
60-61	22.772478472415898	27.381900557247533	27.22086850202289	22.624752468313677
62-63	22.772921824398928	27.477681691714523	27.13426606959017	22.61513041429638
64-65	22.699419577029552	27.44247048502234	27.13491075064977	22.723199187298334
66-67	22.7944281114688	27.443834800630047	27.011723000727418	22.750014087173735
68-69	22.724250237539913	27.413828064351197	27.118481809410593	22.743439888698298
70-71	22.729669808866625	27.23973888305219	27.133891295708256	22.896700012372932
72-73	22.81631229714829	27.230213202615626	27.082224212883116	22.87125028735296
74-75	22.604868598835935	27.361280837191256	27.130881793292584	22.90296877068023
76-77	22.790328973444314	27.292278733248615	27.04278938462593	22.87460290868114
78-79	22.67661857328793	27.303734421433646	27.038262945288032	22.98138405999039
80-81	22.756143307151408	27.272718790984573	27.062693030150843	22.908444871713172
82-83	22.785513691445907	27.258982833567103	27.172364824004298	22.783138650982696
84-85	22.862882871232777	27.207991584090124	27.098523743038466	22.830601801638633
86-87	22.704419815293043	27.25551790713728	27.17673654789311	22.86332572967657
88-89	22.767044205057296	27.193225667125997	27.181221591673165	22.85850853614354
90-91	22.774794417790055	27.223605916389186	27.17317116947443	22.828428496346323
92-93	22.830238783064516	27.130389883304453	27.229189741745984	22.810181591885048
94-95	22.623717653399346	27.214389814955453	27.334438838809184	22.827453692836013
96-97	22.67628203404936	27.35007405728315	27.36608557094864	22.607558337718846
98-99	23.851407282325788	29.285275764455847	27.505139094863125	19.358177858355237
100	22.78778137227268	30.344068376445936	21.178717120892408	25.689433130388974
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1213.0
1	1056.5
2	1410.0
3	2076.5
4	2926.0
5	4827.0
6	6499.5
7	7860.5
8	8964.0
9	9108.5
10	8362.0
11	7435.0
12	7964.5
13	10470.5
14	16183.5
15	24020.0
16	30692.0
17	34734.0
18	35997.0
19	35014.0
20	32790.0
21	30577.5
22	29813.0
23	31923.0
24	36872.5
25	45240.5
26	56895.0
27	69139.0
28	81392.0
29	95459.0
30	108371.5
31	120574.0
32	134444.0
33	148247.0
34	162418.0
35	175905.5
36	191312.5
37	206394.0
38	217786.5
39	228905.0
40	240835.5
41	251509.5
42	259884.5
43	268780.5
44	276848.0
45	284335.0
46	291461.5
47	296286.5
48	300866.0
49	303802.0
50	303261.5
51	299644.5
52	291356.5
53	279095.5
54	267399.0
55	253907.5
56	237762.5
57	219820.0
58	198327.5
59	174755.0
60	150051.0
61	125273.5
62	101755.0
63	81175.0
64	63076.5
65	48610.5
66	37842.0
67	28825.0
68	21442.5
69	15953.5
70	11572.0
71	8086.5
72	5736.0
73	3940.5
74	2692.5
75	1860.0
76	1232.0
77	787.0
78	501.0
79	320.0
80	219.0
81	148.0
82	108.0
83	80.0
84	55.5
85	37.0
86	27.5
87	28.0
88	23.5
89	15.0
90	9.0
91	5.0
92	2.5
93	2.0
94	2.5
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.5802118403262017E-4
18-19	0.0
20-21	8.044968500715967E-4
22-23	5.31588306120442E-4
24-25	7.256921684235244E-4
26-27	0.0024078542913210437
28-29	0.0020282036825705887
30-31	0.0026361938028845867
32-33	0.0013577502079055006
34-35	9.205813725070911E-4
36-37	0.0064938160067972974
38-39	0.0029376219480311177
40-41	7.776905612215045E-4
42-43	1.0460813791536902E-4
44-45	7.095917326222117E-4
46-47	0.0029025046399973847
48-49	2.394964394218865E-4
50-51	0.0013819884893632173
52-53	0.0027913639929184513
54-55	0.0016010661029214677
56-57	1.1274571821961947E-4
58-59	0.001808313992383414
60-61	4.037113763558935E-4
62-63	0.0010496546636156706
64-65	0.002939723703034334
66-67	0.003546176373685155
68-69	0.0018731787221399193
70-71	0.0013640643030405255
72-73	0.0011157494409298337
74-75	0.022914494152680098
76-77	0.007490657107680238
78-79	0.002042400416649685
80-81	0.005355085554022318
82-83	0.01040746059759071
84-85	0.00509372208824955
86-87	0.006871240161522807
88-89	0.00985739990660682
90-91	3.923693221518137E-4
92-93	1.0248946920703897E-5
94-95	3.765901519164673E-4
96-97	0.0037634780903208938
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	131.0
20-21	490.0
22-23	1067.0
24-25	2245.0
26-27	3648.0
28-29	7491.0
30-31	17689.0
32-33	23122.0
34-35	33322.0
36-37	49353.0
38-39	57028.0
40-41	58719.0
42-43	64564.0
44-45	76827.0
46-47	75646.0
48-49	69939.0
50-51	63509.0
52-53	62692.0
54-55	67217.0
56-57	70647.0
58-59	69880.0
60-61	67636.0
62-63	65331.0
64-65	69376.0
66-67	72752.0
68-69	74031.0
70-71	72950.0
72-73	69151.0
74-75	70438.0
76-77	70711.0
78-79	75129.0
80-81	75416.0
82-83	72465.0
84-85	73816.0
86-87	78662.0
88-89	85131.0
90-91	91306.0
92-93	96060.0
94-95	112883.0
96-97	257768.0
98-99	450675.0
100-101	3984179.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.78182975971299
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.99844293988555	78.97011383608104
2	6.375457911976877	11.065478063147852
3	1.5036702176466223	3.914737585276793
4	0.5084269572161532	1.7648888658552433
5	0.22884003765379093	0.9929578594938796
6	0.12270649937698869	0.6389216723606496
7	0.07054687577244283	0.4285530874354628
8	0.04310057932160289	0.2992277709785878
9	0.03526273810054988	0.2754148441232938
>10	0.11128819781496449	1.4600840762962874
>50	0.0018840716905280937	0.10600802430850045
>100	3.218160190365728E-4	0.0485197316784069
>500	2.5578787982851286E-5	0.012874689340288037
>1k	2.5578736919045267E-5	0.02221989362369849
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.4365562184783652E-5	0.0	0.0	0.0
3	0.0	2.8731124369567304E-5	0.0	0.0	0.0
4	0.0	1.0055893529348556E-4	0.0	1.4365562184783652E-5	0.0
5	0.0	1.0055893529348556E-4	0.0	1.4365562184783652E-5	0.0
6	0.0	1.0055893529348556E-4	0.0	1.4365562184783652E-5	0.0
7	0.0	1.0055893529348556E-4	0.0	1.4365562184783652E-5	0.0
8	0.0	1.2929005966305288E-4	0.0	1.4365562184783652E-5	0.0
9	0.0	3.878701789891586E-4	0.0	2.8731124369567304E-5	0.0
10-11	0.0	4.0223574117394223E-4	0.0	3.5913905461959134E-5	0.0
12-13	0.0	4.166013033587259E-4	0.0	7.182781092391825E-5	0.0
14-15	0.0	6.464502983152643E-4	0.0	8.619337310870191E-5	0.0
16-17	0.0	6.60815860500048E-4	0.0	1.364728407554447E-4	0.0
18-19	0.0	7.03912547054399E-4	0.0	1.7238674621740383E-4	0.0
20-21	0.0	7.613747957935335E-4	0.0	1.7956952730979566E-4	0.0
22-23	0.0	8.403853878098436E-4	0.0	2.1548343277175478E-4	0.0
24-25	0.0	9.337615420109374E-4	0.0	2.442145571413221E-4	0.0
26-27	0.0	9.624926663805047E-4	0.0	2.729456815108894E-4	0.0
28-29	0.0	9.696754474728965E-4	0.0	6.60815860500048E-4	0.0
30-31	0.0	0.0010917827260435574	0.0	0.001903436989483834	0.0
32-33	0.0	0.0010917827260435574	0.0	0.004309668655435096	0.0
34-35	7.182781092391826E-6	0.0012067072235218267	0.0	0.0072905228087777035	0.0
36-37	1.4365562184783652E-5	0.0013359972831848796	0.0	0.011743847086060636	0.0
38-39	1.4365562184783652E-5	0.0013862767508316224	0.0	0.01872551030786549	0.0
40-41	1.4365562184783652E-5	0.0016017601836033771	0.0	0.026339258265800823	0.0
42-43	1.4365562184783652E-5	0.0016376740890653363	0.0	0.03392427509936659	0.0
44-45	2.154834327717548E-5	0.0018459747407446993	0.0	0.042529246848052	0.0
46-47	2.8731124369567304E-5	0.002025544268054495	0.0	0.052017700671101605	0.0
48-49	2.8731124369567304E-5	0.002032727049146887	0.0	0.06062267241978701	0.0
50-51	2.8731124369567304E-5	0.0022841243873806005	0.0	0.0690624402033474	0.0
52-53	2.8731124369567304E-5	0.002298489949565384	0.0	0.07762431526547847	0.0
54-55	2.8731124369567304E-5	0.0023200382928425596	0.0	0.0862005558897943	0.0
56-57	2.8731124369567304E-5	0.002341586636119735	0.0	0.09409443231033292	0.0
58-59	2.8731124369567304E-5	0.0023631349793969106	0.0	0.10116228890524648	0.0
60-61	2.8731124369567304E-5	0.0024205972281360452	0.0	0.1086108328980568	0.0
62-63	2.8731124369567304E-5	0.0024852422579675717	0.0	0.11571460339843231	0.0
64-65	2.8731124369567304E-5	0.002686360128554543	0.0	0.1224951487496502	0.0
66-67	2.8731124369567304E-5	0.0026935429096469348	0.0	0.12929005966305285	0.0
68-69	2.8731124369567304E-5	0.0027797362827556367	0.0	0.13642256128779795	0.0
70-71	2.8731124369567304E-5	0.0027941018449404204	0.0	0.14242018349994512	0.0
72-73	2.8731124369567304E-5	0.002815650188217596	0.0	0.1474481302646194	0.0
74-75	2.8731124369567304E-5	0.0029090263424186896	0.0	0.1512334559003099	0.0
76-77	2.8731124369567304E-5	0.0030239508398969585	0.0	0.15468837360575036	0.0
78-79	2.8731124369567304E-5	0.0030598647453589176	0.0	0.15696531521203858	0.0
80-81	2.8731124369567304E-5	0.0030670475264513095	0.0	0.15776260391329405	0.0
82-83	2.8731124369567304E-5	0.003081413088636093	0.0	0.15820075355992996	0.0
84-85	2.8731124369567304E-5	0.0031101442130056604	0.0	0.15841623699270171	0.0
86-87	2.8731124369567304E-5	0.0031604236806524032	0.0	0.15855989261454956	0.0
88	2.8731124369567304E-5	0.0032322514915763215	0.0	0.15856707539564194	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7830	0.0	44.822674	1
GTATCAA	13975	0.0	35.17077	1
TCAACGC	16525	0.0	28.993044	4
ATCAACG	16715	0.0	28.68821	3
CAACGCA	17145	0.0	27.992811	5
TATCAAC	17410	0.0	27.804173	2
AACGCAG	17510	0.0	27.64538	6
ACGCAGA	20625	0.0	23.409962	7
CGCAGAG	21615	0.0	22.318626	8
TGGTATC	4100	0.0	22.181486	2
GTGGTAT	4395	0.0	21.821316	1
GTACATG	19940	0.0	21.042276	1
TACATGG	19900	0.0	20.461384	2
ACATGGG	20370	0.0	19.36017	3
GCAGAGT	25075	0.0	19.22248	9
GAGTACT	13805	0.0	18.236263	12-13
CAGAGTA	24000	0.0	17.258833	10-11
AGAGTAC	22250	0.0	17.213547	10-11
CATGGGG	16075	0.0	17.15247	4
GTACTTT	15725	0.0	16.1284	14-15
>>END_MODULE