FastQCFastQC Report
Fri 27 May 2016
SRR2138702_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138702_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4098365
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41400.044.7347221
GTATCAA71050.031.9356561
TCAACGC79100.028.1170234
ATCAACG80850.027.5084293
CAACGCA82250.027.0899075
AACGCAG85300.026.2650646
TATCAAC86650.026.186132
TAAGGCG16500.025.77552818-19
TCTCCGA23200.025.552121
GCGAATC15800.024.2270522-23
CGAGACT18550.023.36193812-13
GTACATG117200.022.3951681
ACGCAGA101500.022.1132837
GCCCACG27600.022.0711088
TACATGG119100.021.8319362
CCCACGA28300.021.6696419
CGCAGAG103850.021.6128868
ACATGGG120850.021.1774963
TCCGAGC28800.021.1514763
TATGCCG18800.020.04516632-33
Produced by FastQC (version 0.11.5)