FastQCFastQC Report
Fri 27 May 2016
SRR2138701_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138701_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6247050
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69000.046.1902851
GTATCAA116800.033.2438551
TCAACGC137300.027.4609994
ATCAACG139550.027.048093
CAACGCA143700.026.1799775
TATCAAC147200.025.7839132
AACGCAG147100.025.7731196
GTACATG190300.022.1116371
TACATGG190150.021.559422
ACGCAGA176150.021.475417
ACATGGG195450.020.8255833
CGCAGAG182100.020.7279598
GTGGTAT36800.018.9063171
CATGGGG147100.018.0411834
TGGTATC36800.017.8874132
GCAGAGT214600.017.511179
GAGTACT112350.016.94655412-13
CAGAGTA207200.016.53805510-11
AGAGTAC190300.016.22249810-11
ATGGGGG88800.015.3886025
Produced by FastQC (version 0.11.5)