##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138701_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6247050
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18353911046014	33.0	31.0	34.0	30.0	34.0
2	32.44244115222385	34.0	31.0	34.0	31.0	34.0
3	32.464597850185285	34.0	31.0	34.0	31.0	34.0
4	36.094183174458344	37.0	35.0	37.0	35.0	37.0
5	35.845878614706145	37.0	35.0	37.0	35.0	37.0
6	35.94104433292514	37.0	35.0	37.0	35.0	37.0
7	35.90336911022003	37.0	35.0	37.0	35.0	37.0
8	35.91161332148774	37.0	35.0	37.0	35.0	37.0
9	37.70321319662881	39.0	37.0	39.0	35.0	39.0
10-11	37.61436173874068	39.0	37.0	39.0	35.0	39.0
12-13	37.579473511497426	39.0	37.0	39.0	35.0	39.0
14-15	38.880507679624785	40.0	38.0	41.0	35.0	41.0
16-17	38.83774117383405	40.0	38.0	41.0	35.0	41.0
18-19	38.83631241946198	40.0	38.0	41.0	35.0	41.0
20-21	38.84866557017525	40.0	38.0	41.0	35.0	41.0
22-23	38.77358436976391	40.0	38.0	41.0	34.0	41.0
24-25	38.70179564919681	40.0	38.0	41.0	34.0	41.0
26-27	38.54771985891277	40.0	38.0	41.0	34.0	41.0
28-29	38.42134746332738	40.0	38.0	41.0	34.0	41.0
30-31	38.296282427233564	40.0	38.0	41.0	34.0	41.0
32-33	38.14467736954512	40.0	38.0	41.0	33.5	41.0
34-35	38.106093219153806	40.0	37.5	41.0	33.0	41.0
36-37	38.116712863170946	40.0	38.0	41.0	33.0	41.0
38-39	38.08057319904064	40.0	37.5	41.0	33.0	41.0
40-41	38.11868650522179	40.0	37.5	41.0	33.0	41.0
42-43	38.18263337460304	40.0	38.0	41.0	33.0	41.0
44-45	38.16596862663465	40.0	38.0	41.0	33.0	41.0
46-47	38.07222064831788	40.0	37.0	41.0	33.0	41.0
48-49	37.991165277585125	40.0	37.0	41.0	33.0	41.0
50-51	37.89401855950386	40.0	37.0	41.0	33.0	41.0
52-53	37.72990756725354	40.0	37.0	41.0	33.0	41.0
54-55	37.60162354126932	39.5	36.0	41.0	32.0	41.0
56-57	37.441878629310835	39.0	36.0	41.0	32.0	41.0
58-59	37.217431033011216	39.0	35.5	41.0	31.5	41.0
60-61	37.02480515662741	39.0	35.0	40.5	31.0	41.0
62-63	36.79235715570276	38.5	35.0	40.0	31.0	41.0
64-65	36.4856852936348	38.0	35.0	40.0	31.0	41.0
66-67	36.156080168180964	37.0	35.0	40.0	30.5	41.0
68-69	35.89394679795374	37.0	34.0	39.5	30.0	41.0
70-71	35.50102306013955	36.0	34.0	39.0	30.0	41.0
72-73	35.208572894637626	36.0	34.0	39.0	30.0	40.0
74-75	34.885656688100134	35.0	34.0	38.0	29.5	40.0
76-77	33.513038970683766	34.5	32.0	36.5	28.0	39.0
78-79	34.26917192406327	35.0	33.5	37.0	29.0	39.0
80-81	34.222489311964154	35.0	34.0	36.5	30.0	39.0
82-83	33.9874052733344	35.0	34.0	36.0	29.0	37.5
84-85	33.79171576547786	35.0	33.5	36.0	29.0	37.0
86-87	33.56763044864843	35.0	33.0	35.0	29.0	37.0
88-89	33.46352708711636	35.0	33.0	35.0	29.0	36.0
90-91	33.36954337140719	35.0	33.0	35.0	29.0	36.0
92-93	33.20293887496578	35.0	33.0	35.0	29.0	36.0
94-95	33.226956014511686	35.0	33.0	35.0	29.0	35.5
96-97	33.27791985299454	35.0	33.0	35.0	29.0	35.0
98-99	33.368347832196584	35.0	33.0	35.0	30.0	35.0
100	32.216902331287805	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	8.0
20	24.0
21	163.0
22	792.0
23	2729.0
24	7595.0
25	16139.0
26	30342.0
27	48333.0
28	71258.0
29	96070.0
30	123790.0
31	155478.0
32	194572.0
33	247712.0
34	332636.0
35	466818.0
36	735506.0
37	1284736.0
38	1580467.0
39	817634.0
40	34245.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.610416116406945	19.63942981087073	13.460641422751538	24.28951264997079
2	15.670436446002514	20.735195012045686	39.815064710543375	23.779303831408424
3	19.18595176923508	24.77119600451413	29.746040130941804	26.296812095308987
4	13.122561849192818	17.04631786203088	37.459536901417465	32.37158338735883
5	13.861598674574399	37.37948311603076	34.9885305864368	13.770387622958037
6	31.80507599587005	36.583603460833515	17.919946214613297	13.691374328683139
7	27.984616739100854	31.93640198173538	21.95074475152272	18.128236527641047
8	25.479210187208363	36.11829583563442	20.385894142035042	18.016599835122175
9	25.79407880519605	16.370767002024955	20.679488718675216	37.15566547410378
10-11	25.125435205416956	26.29196180597242	28.059556110484145	20.523046878126475
12-13	25.49223233366149	23.655245275770163	28.40414275538054	22.44837963518781
14-15	22.511945638341295	25.252543200390587	25.8452469565635	26.39026420470462
16-17	22.3804963552724	28.44501093518328	26.605635071023663	22.568857638520658
18-19	22.362827254464108	27.63706069264693	28.21165990347444	21.788452149414525
20-21	23.133643466943717	26.81429762901468	28.14721848251176	21.904840421529848
22-23	22.87000221652761	26.68883354373936	27.979270463837018	22.461893775896012
24-25	22.60431513670172	26.850676234722247	28.06309645221969	22.481912176356342
26-27	22.564055040723396	27.023953304915953	28.01718658978879	22.39480506457186
28-29	22.500568424665037	27.062423807652625	28.029102381116296	22.40790538656604
30-31	22.705091578198953	26.956180682723534	27.909286086275877	22.42944165280164
32-33	22.51146785761553	27.078577603494324	27.883892140389182	22.52606239850096
34-35	22.64915531217247	27.13555458161419	27.90499965327221	22.31029045294113
36-37	22.735108400213186	27.046335191882363	27.75870866221472	22.459847745689732
38-39	22.50098582254377	27.20496731972728	27.88079413369184	22.41325272403711
40-41	22.75956296757119	27.044231772961403	27.74078598090438	22.45541927856303
42-43	22.574364984018448	27.15041678276841	27.616578955345922	22.65863927786722
44-45	22.687312822175187	27.212449540543602	27.490133244072968	22.610104393208243
46-47	22.761299578103422	27.243222770734203	27.253530504917066	22.741947146245302
48-49	22.650194937961977	27.343820218557745	27.236872736351835	22.769112107128443
50-51	22.663458861947145	27.462771415870257	27.190048580999953	22.683721141182644
52-53	22.831482606870225	27.37360529262478	27.05046613666439	22.744445963840604
54-55	22.776452407858606	27.279124316561564	27.14826162058182	22.79616165499801
56-57	22.760139689167882	27.397262961131453	27.08778080006876	22.7548165496319
58-59	22.72117648246773	27.442746770219667	27.088258904615454	22.74781784269715
60-61	22.812761809070143	27.405280258292763	27.12294572162509	22.659012211012
62-63	22.79594986593999	27.460342959297858	27.045414602280033	22.698292572482124
64-65	22.75521904444677	27.419690126319825	27.08331452064032	22.741776308593085
66-67	22.844753147045825	27.448100416191057	26.894098689955836	22.813047746807282
68-69	22.749841213926207	27.433353691499818	27.01512489773262	22.80168019684136
70-71	22.776794395311775	27.26324102189606	27.05974453756343	22.900220045228732
72-73	22.80451395840556	27.310346848188605	26.992729030651358	22.892410162754473
74-75	22.63953328726514	27.42372233672275	27.019507087506266	22.91723728850584
76-77	22.795725668250764	27.322043658739776	26.96926092006243	22.912969752947035
78-79	22.666670989780666	27.368503284232553	26.970141839006086	22.994683886980695
80-81	22.733130185166623	27.358105743303753	26.98650727011914	22.92225680141048
82-83	22.7466101171721	27.386759335210982	27.048309818992134	22.818320728624787
84-85	22.83453792397965	27.376392921130034	26.959658645041777	22.82941050984854
86-87	22.696744030701556	27.387882521806006	27.0541583191041	22.861215128388338
88-89	22.73371140965618	27.33152103734198	27.019450053492456	22.915317499509385
90-91	22.72293534225069	27.390168383178132	27.017034837537256	22.86986143703392
92-93	22.82032544325836	27.359108519705483	27.03658250304507	22.78398353399109
94-95	22.629230186443117	27.43627964232356	27.09327428222008	22.84121588901325
96-97	22.651992014320317	27.539274626242577	27.18464740359393	22.624085955843167
98-99	23.825378868763096	29.495109644576097	27.342003802259747	19.337507684401068
100	22.763971126594885	30.546098066677953	21.017594044145614	25.672336762581544
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1306.0
1	1109.0
2	1344.5
3	1855.0
4	2483.0
5	4011.0
6	5405.0
7	6498.0
8	7296.0
9	7310.5
10	6599.0
11	5841.0
12	6201.0
13	8122.0
14	12825.0
15	19044.0
16	24122.5
17	27363.0
18	28390.0
19	27514.5
20	25900.5
21	24210.5
22	23441.0
23	24875.0
24	28791.0
25	35437.0
26	44312.0
27	54071.0
28	64731.5
29	77214.0
30	90090.5
31	102949.0
32	116577.0
33	130122.5
34	143859.5
35	157299.0
36	171389.5
37	185195.0
38	196989.5
39	208267.0
40	219879.0
41	229983.5
42	237978.0
43	245828.0
44	253867.5
45	261040.0
46	265815.0
47	270154.0
48	273683.0
49	275311.5
50	275087.0
51	271338.5
52	263841.0
53	253941.5
54	242508.0
55	228540.5
56	213031.0
57	196089.0
58	176696.0
59	154998.0
60	131615.5
61	109361.0
62	89230.0
63	70803.5
64	54817.5
65	42673.0
66	33504.0
67	25542.5
68	18989.0
69	14040.0
70	10099.0
71	7044.5
72	4993.0
73	3369.0
74	2225.0
75	1505.5
76	1000.5
77	636.5
78	418.0
79	292.5
80	207.0
81	142.5
82	95.0
83	59.0
84	38.5
85	27.5
86	20.0
87	16.5
88	11.5
89	6.5
90	4.0
91	3.5
92	3.5
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	2.0009444457784077E-4
18-19	0.0
20-21	8.804472672117435E-4
22-23	5.203089386354041E-4
24-25	7.766218566530592E-4
26-27	0.0023147087647849016
28-29	0.001955486867743611
30-31	0.0024959234587752896
32-33	0.0012470372807829142
34-35	0.0012674173006174986
36-37	0.0065155328111440765
38-39	0.0032117373390535497
40-41	7.496240141311541E-4
42-43	1.1627463703833882E-4
44-45	7.378683336006709E-4
46-47	0.00308544364822857
48-49	2.9131579892115476E-4
50-51	0.0013069475340193681
52-53	0.002839685345388744
54-55	0.001579185105196666
56-57	1.7832931468222697E-4
58-59	0.002028488818203316
60-61	4.3748582728205367E-4
62-63	0.0013724520864541651
64-65	0.003295409745569233
66-67	0.003983026469235358
68-69	0.0018775671799732014
70-71	0.0017175661277433053
72-73	0.0012305317186152886
74-75	0.022580898911491967
76-77	0.007923027388765247
78-79	0.0025127468710475533
80-81	0.005925260185157706
82-83	0.011513183063045919
84-85	0.005517468378734643
86-87	0.007851717638113478
88-89	0.009262985609034245
90-91	2.539293356612397E-4
92-93	2.247489245483024E-5
94-95	4.6993095224286585E-4
96-97	0.0037340186651460454
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	148.0
20-21	430.0
22-23	953.0
24-25	2158.0
26-27	3174.0
28-29	6730.0
30-31	14440.0
32-33	19167.0
34-35	28007.0
36-37	41520.0
38-39	48216.0
40-41	49191.0
42-43	54266.0
44-45	63680.0
46-47	64187.0
48-49	60063.0
50-51	55021.0
52-53	53851.0
54-55	58782.0
56-57	61745.0
58-59	60688.0
60-61	58249.0
62-63	56730.0
64-65	59798.0
66-67	63722.0
68-69	64367.0
70-71	63008.0
72-73	60112.0
74-75	61415.0
76-77	62162.0
78-79	65304.0
80-81	66533.0
82-83	63677.0
84-85	64609.0
86-87	68343.0
88-89	74246.0
90-91	78851.0
92-93	83521.0
94-95	102466.0
96-97	234500.0
98-99	410516.0
100-101	3638504.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.66173561350853
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.28317086399498	80.02041190242252
2	6.344990383849845	11.124257389985983
3	1.3778311626490711	3.623492133005878
4	0.4698938854086084	1.6476685419637478
5	0.21193820436574076	0.9289435418755652
6	0.1041816847213676	0.5479648381088656
7	0.06168412665214063	0.3785136321491089
8	0.03691876256210816	0.25890902423179385
9	0.02656111692154449	0.20955542482583284
>10	0.08079129071077706	1.1109586194029353
>50	0.0016972084354880902	0.09563208627667374
>100	3.143000579350293E-4	0.03721357222950472
>500	2.700967040935916E-5	0.016479293521575403
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.600755556622726E-5
2	0.0	1.600755556622726E-5	0.0	0.0	1.600755556622726E-5
3	0.0	1.600755556622726E-5	0.0	0.0	1.600755556622726E-5
4	0.0	8.00377778311363E-5	0.0	1.600755556622726E-5	4.802266669868178E-5
5	0.0	1.1205288896359081E-4	0.0	1.600755556622726E-5	4.802266669868178E-5
6	0.0	1.1205288896359081E-4	0.0	1.600755556622726E-5	4.802266669868178E-5
7	0.0	1.1205288896359081E-4	0.0	1.600755556622726E-5	6.403022226490904E-5
8	0.0	1.2806044452981808E-4	0.0	1.600755556622726E-5	6.403022226490904E-5
9	0.0	3.521662224569997E-4	0.0	4.802266669868178E-5	9.604533339736356E-5
10-11	0.0	3.521662224569997E-4	0.0	1.1205288896359081E-4	9.604533339736356E-5
12-13	0.0	3.521662224569997E-4	0.0	1.2806044452981808E-4	9.604533339736356E-5
14-15	0.0	5.202455559023859E-4	0.0	1.7608311122849985E-4	1.2005666674670446E-4
16-17	0.0	5.362531114686132E-4	0.0	2.0809822236095437E-4	1.2806044452981808E-4
18-19	0.0	5.682682226010677E-4	0.0	2.3210955571029525E-4	1.7608311122849985E-4
20-21	0.0	5.922795559504086E-4	0.0	2.481171112765225E-4	1.920906667947271E-4
22-23	0.0	6.242946670828631E-4	0.0	3.121473335414316E-4	2.401133334934089E-4
24-25	0.0	6.723173337815449E-4	0.0	3.521662224569997E-4	2.721284446258634E-4
26-27	0.0	7.203400004802266E-4	0.0	4.482115558543633E-4	2.721284446258634E-4
28-29	0.0	7.203400004802266E-4	0.0	8.564042227931583E-4	2.801322224089771E-4
30-31	0.0	7.683626671789084E-4	0.0	0.0019289104457303847	2.881360001920907E-4
32-33	0.0	8.323928894438175E-4	0.0	0.004081926669387951	3.0414355575831794E-4
34-35	0.0	9.444457784074083E-4	0.0	0.007451517116078789	3.201511113245452E-4
36-37	0.0	0.001056498667370999	0.0	0.013022146453125875	3.201511113245452E-4
38-39	0.0	0.0010725062229372264	0.0	0.02088185623614346	3.521662224569997E-4
40-41	0.0	0.0012645968897319535	0.0	0.02946190601964127	3.521662224569997E-4
42-43	0.0	0.0012886082230812944	0.0	0.037721804691814534	3.601700002401133E-4
44-45	0.0	0.0014326762231773398	0.0	0.04736635692046646	3.6817377802322697E-4
46-47	0.0	0.0015527328899240442	0.0	0.058243490927717884	3.841813335894542E-4
48-49	0.0	0.0015607366677071579	0.0	0.06873644360137984	4.2420022250502234E-4
50-51	0.0	0.0017848424456343394	0.0	0.07910933960829511	4.32204000288136E-4
52-53	0.0	0.0018008500012005667	0.0	0.08903402405935601	4.4020777807124965E-4
54-55	0.0	0.0018728840012485893	0.0	0.09895870851041691	4.4821155585436325E-4
56-57	0.0	0.0019529217790797257	0.0	0.10897143451709207	4.5621533363747685E-4
58-59	0.0	0.0019769331124290662	0.0	0.11794366941196244	4.7222288920370416E-4
60-61	0.0	0.0020169520013446347	0.0	0.12629160963974997	5.042380003361587E-4
62-63	0.0	0.002056970890260203	0.0	0.1342953874228636	5.60264444817954E-4
64-65	0.0	0.002273072890404271	0.0	0.14195500276130335	5.60264444817954E-4
66-67	0.0	0.002273072890404271	0.0	0.14944653876629768	5.682682226010677E-4
68-69	0.0	0.002289080445970498	0.0	0.157426305216062	5.762720003841814E-4
70-71	0.0	0.0023050880015367254	0.0	0.16363723677575814	5.922795559504086E-4
72-73	0.0	0.0023210955571029528	0.0	0.16846351477897567	6.403022226490904E-4
74-75	0.0	0.0023691182238016343	0.0	0.17232933944821954	6.483060004322039E-4
76-77	0.0	0.0025211900016807935	0.0	0.17645128500652307	6.563097782153176E-4
78-79	0.0	0.0025932240017288158	0.0	0.17880439567475848	6.563097782153176E-4
80-81	0.0	0.002617235335078157	0.0	0.17960477345306985	6.803211115646586E-4
82-83	0.0	0.0026252391128612704	0.0	0.18022106434236962	7.203400004802266E-4
84-85	0.0	0.0026492504462106116	0.0	0.180461177675863	7.283437782633403E-4
86-87	0.0	0.0027532995573910884	0.0	0.1805732305648266	7.363475560464539E-4
88	0.0	0.0028173297796559977	0.0	0.18058123434260973	7.523551116126811E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6900	0.0	46.190285	1
GTATCAA	11680	0.0	33.243855	1
TCAACGC	13730	0.0	27.460999	4
ATCAACG	13955	0.0	27.04809	3
CAACGCA	14370	0.0	26.179977	5
TATCAAC	14720	0.0	25.783913	2
AACGCAG	14710	0.0	25.773119	6
GTACATG	19030	0.0	22.111637	1
TACATGG	19015	0.0	21.55942	2
ACGCAGA	17615	0.0	21.47541	7
ACATGGG	19545	0.0	20.825583	3
CGCAGAG	18210	0.0	20.727959	8
GTGGTAT	3680	0.0	18.906317	1
CATGGGG	14710	0.0	18.041183	4
TGGTATC	3680	0.0	17.887413	2
GCAGAGT	21460	0.0	17.51117	9
GAGTACT	11235	0.0	16.946554	12-13
CAGAGTA	20720	0.0	16.538055	10-11
AGAGTAC	19030	0.0	16.222498	10-11
ATGGGGG	8880	0.0	15.388602	5
>>END_MODULE