FastQCFastQC Report
Fri 27 May 2016
SRR2138700_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138700_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5284316
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62200.044.8188171
GTATCAA105650.034.161051
TCAACGC123950.028.3142644
ATCAACG125800.027.9968093
CAACGCA128350.027.3759385
TATCAAC129350.027.292582
AACGCAG132250.026.7882086
ACGCAGA156400.022.6517937
CGCAGAG158400.022.3134068
GTGGTAT32450.021.6049611
GTACATG154150.020.775681
TACATGG154800.020.1524732
TGGTATC32400.019.333632
ACATGGG157050.019.2562223
GCAGAGT186250.019.0436979
GAGTACT103100.018.0260612-13
CATGGGG116900.017.5304834
CAGAGTA179600.017.25423610-11
AGAGTAC164850.017.06169710-11
GTACTTT113350.016.34141514-15
Produced by FastQC (version 0.11.5)