##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138700_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5284316
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.172423072352224	33.0	31.0	34.0	30.0	34.0
2	32.43311433305654	34.0	31.0	34.0	31.0	34.0
3	32.454331080881616	34.0	31.0	34.0	31.0	34.0
4	36.08433049802472	37.0	35.0	37.0	35.0	37.0
5	35.834271076900016	37.0	35.0	37.0	35.0	37.0
6	35.929053069498494	37.0	35.0	37.0	35.0	37.0
7	35.89263813897579	37.0	35.0	37.0	35.0	37.0
8	35.904626634743266	37.0	35.0	37.0	35.0	37.0
9	37.69120714961028	39.0	37.0	39.0	35.0	39.0
10-11	37.60463397722619	39.0	37.0	39.0	35.0	39.0
12-13	37.5664321550793	39.0	37.0	39.0	35.0	39.0
14-15	38.864843434798374	40.0	38.0	41.0	35.0	41.0
16-17	38.82186228075687	40.0	38.0	41.0	35.0	41.0
18-19	38.82195888739432	40.0	38.0	41.0	35.0	41.0
20-21	38.833754085864086	40.0	38.0	41.0	35.0	41.0
22-23	38.7588837067408	40.0	38.0	41.0	34.0	41.0
24-25	38.68542704694383	40.0	38.0	41.0	34.0	41.0
26-27	38.529931879326384	40.0	38.0	41.0	34.0	41.0
28-29	38.40296015727674	40.0	38.0	41.0	34.0	41.0
30-31	38.271110400003764	40.0	38.0	41.0	34.0	41.0
32-33	38.11838157932451	40.0	38.0	41.0	33.5	41.0
34-35	38.07880951832479	40.0	37.5	41.0	33.0	41.0
36-37	38.09317000087752	40.0	37.5	41.0	33.0	41.0
38-39	38.055081535512315	40.0	37.0	41.0	33.0	41.0
40-41	38.09406847994734	40.0	37.5	41.0	33.0	41.0
42-43	38.15220504149343	40.0	38.0	41.0	33.0	41.0
44-45	38.1322316404629	40.0	37.5	41.0	33.0	41.0
46-47	38.036142430793404	40.0	37.0	41.0	33.0	41.0
48-49	37.95827886079482	40.0	37.0	41.0	33.0	41.0
50-51	37.861444940826544	40.0	37.0	41.0	33.0	41.0
52-53	37.696130029654896	40.0	36.5	41.0	32.5	41.0
54-55	37.569161262587095	39.5	36.0	41.0	32.0	41.0
56-57	37.406751570480466	39.0	36.0	41.0	32.0	41.0
58-59	37.18335798855361	39.0	35.0	41.0	31.5	41.0
60-61	36.991503244912366	39.0	35.0	40.5	31.0	41.0
62-63	36.75852685004301	38.0	35.0	40.0	31.0	41.0
64-65	36.45348234220605	38.0	35.0	40.0	31.0	41.0
66-67	36.12105625486351	37.0	35.0	40.0	30.5	41.0
68-69	35.860738610176895	37.0	34.0	39.5	30.0	41.0
70-71	35.468175330652585	36.0	34.0	39.0	30.0	41.0
72-73	35.17860793627402	36.0	34.0	39.0	30.0	40.0
74-75	34.858302670581146	35.0	34.0	38.0	29.0	40.0
76-77	33.485043709850686	34.5	32.0	36.5	28.0	39.0
78-79	34.25028716207877	35.0	33.5	37.0	29.0	39.0
80-81	34.2037222391359	35.0	34.0	36.5	29.0	39.0
82-83	33.970092247705736	35.0	34.0	36.0	29.0	37.5
84-85	33.77419973666687	35.0	33.0	36.0	29.0	37.0
86-87	33.549322889147106	35.0	33.0	35.0	29.0	37.0
88-89	33.44413390499065	35.0	33.0	35.0	29.0	36.0
90-91	33.34631312973649	35.0	33.0	35.0	29.0	36.0
92-93	33.183153589417444	35.0	33.0	35.0	29.0	36.0
94-95	33.20695609892256	35.0	33.0	35.0	29.0	35.5
96-97	33.25770341728116	35.0	33.0	35.0	29.0	35.0
98-99	33.3520632810599	35.0	33.0	35.0	29.5	35.0
100	32.17832737347358	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	0.0
18	2.0
19	7.0
20	27.0
21	161.0
22	674.0
23	2443.0
24	6481.0
25	14423.0
26	26199.0
27	42382.0
28	61773.0
29	83046.0
30	106456.0
31	133362.0
32	166086.0
33	210632.0
34	282381.0
35	395968.0
36	622021.0
37	1080972.0
38	1322700.0
39	697186.0
40	28933.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.62971782913815	19.627951848451154	13.5041507737236	24.238179548687096
2	15.649612930036735	20.69802411513619	39.89341288446792	23.758950070359152
3	19.177903062572337	24.68168065649367	29.8186936587441	26.321722622189892
4	13.083812550195711	17.046860937158186	37.606418692599	32.2629078200471
5	13.811286077516938	37.33279766009451	35.08420011218103	13.771716150207519
6	31.753740692267456	36.713436516665546	17.850616806413544	13.682205984653454
7	27.900905244879375	31.951325393863648	21.977357145182083	18.17041221607489
8	25.468007590764824	36.12189354308107	20.36959939564553	18.04049947050858
9	25.715172976029443	16.394704631592813	20.745844873773635	37.14427751860411
10-11	25.097912388282605	26.285180522890762	28.115701256321536	20.501205832505097
12-13	25.438855284203292	23.701336180500938	28.51096527913925	22.34884325615652
14-15	22.464799606987924	25.252653323533263	25.93575970854128	26.346787360937533
16-17	22.34921560692378	28.419338121534725	26.711195987138147	22.520250284403343
18-19	22.31643603448393	27.66701499304735	28.27294961164321	21.74359936082551
20-21	23.03221248624876	26.795524433916363	28.248221209829016	21.924041870005862
22-23	22.79750347751278	26.675274451204057	28.090638002819862	22.436584068463304
24-25	22.554936752089183	26.79569821636755	28.20915601334329	22.440209018199976
26-27	22.4996513067074	26.94333423887853	28.147450533607266	22.409563920806804
28-29	22.466505453567944	27.07936613518648	28.103269241319634	22.35085916992594
30-31	22.66317820821204	26.936716952040562	27.99417687702814	22.40592796271926
32-33	22.432391676121295	27.076257822367154	28.02563096265957	22.46571953885198
34-35	22.598048852868214	27.1055017888173	27.978593964492532	22.317855393821954
36-37	22.667422214448727	27.059758863399125	27.870113618233596	22.40270530391855
38-39	22.426325687875963	27.21646772422191	27.997626521446662	22.359580066455464
40-41	22.67646866652949	27.051062132534714	27.86905485173638	22.403414349199416
42-43	22.53270688031847	27.14044315082485	27.70091474716177	22.62593522169491
44-45	22.62978946251692	27.2362380992304	27.56360487279423	22.570367565458454
46-47	22.664874528202166	27.237309437082036	27.391755278850553	22.706060755865245
48-49	22.600992783029422	27.37876873202844	27.31088040051068	22.709358084431464
50-51	22.609195022341726	27.44413644396254	27.27603196643947	22.670636567256267
52-53	22.780159546238558	27.361070800716465	27.126084234443415	22.732685418601566
54-55	22.76553233777508	27.245952778895692	27.209675723960192	22.778839159369028
56-57	22.70048958154969	27.38791913977912	27.21332408537611	22.698267193295077
58-59	22.695647642100486	27.45404896770498	27.165902925197067	22.684400464997466
60-61	22.727387825108348	27.384264945380178	27.229132737808605	22.659214491702873
62-63	22.735966147439036	27.48888592097795	27.13909172955885	22.636056202024164
64-65	22.73600196023752	27.442761351918143	27.103611954352825	22.717624733491512
66-67	22.77626352756706	27.44426982462044	27.0308355140923	22.748631133720203
68-69	22.701626492816544	27.394282043801972	27.121584256368536	22.78250720701295
70-71	22.715174735289178	27.221898816748823	27.169397034578235	22.893529413383757
72-73	22.75591312718473	27.275696387505597	27.11145462632677	22.8569358589829
74-75	22.636553473614576	27.38850598382701	27.11915207823064	22.855788464327766
76-77	22.76034878530931	27.278139256386524	27.06452437788177	22.896987580422394
78-79	22.68317501674854	27.315330331332994	27.056128759923464	22.945365891995007
80-81	22.703277404899833	27.32525460080119	27.055245294601814	22.91622269969716
82-83	22.765323434502967	27.280219378590864	27.170525127466604	22.783932059439564
84-85	22.845605725169897	27.221830242680333	27.157941553652876	22.774622478496898
86-87	22.70160917479006	27.242032899782746	27.223106825842674	22.833251099584515
88-89	22.737873915600616	27.197290742884313	27.206409728573718	22.858425612941353
90-91	22.726861362990018	27.22108790115701	27.225376134638957	22.826674601214016
92-93	22.772656560096546	27.159378816605095	27.25853603384259	22.809428589455766
94-95	22.62751303701705	27.21034010994411	27.34552513641864	22.8166217166202
96-97	22.636672795796045	27.330696262542126	27.386940038010906	22.645690903650927
98-99	23.81921161370354	29.24886216927074	27.570754513405504	19.36117170362022
100	22.78624454098372	30.302649862145802	21.21502468132588	25.696080915544595
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	806.0
1	720.0
2	998.5
3	1446.0
4	1936.0
5	3167.0
6	4338.0
7	5331.5
8	6227.5
9	6364.5
10	5682.5
11	4924.0
12	5209.5
13	6846.5
14	11098.0
15	16954.5
16	21602.5
17	24241.0
18	25011.5
19	24349.5
20	22937.5
21	21682.0
22	21372.0
23	22695.5
24	26025.0
25	31894.0
26	39852.0
27	48783.5
28	58400.5
29	69175.5
30	79955.5
31	90651.5
32	102039.5
33	113456.5
34	124454.0
35	134741.5
36	146326.5
37	157666.5
38	167420.5
39	176017.0
40	184142.5
41	191700.0
42	198101.0
43	204680.0
44	211045.0
45	217652.5
46	223037.5
47	226325.0
48	229566.0
49	230797.0
50	229603.5
51	226413.5
52	219568.5
53	211109.5
54	202990.0
55	193409.5
56	181218.5
57	166820.0
58	150855.0
59	133497.0
60	114571.0
61	95373.0
62	77347.0
63	61648.5
64	48135.5
65	37453.0
66	29170.5
67	22263.5
68	16767.0
69	12538.5
70	9147.0
71	6541.5
72	4661.0
73	3199.5
74	2234.0
75	1549.0
76	1034.5
77	676.0
78	457.0
79	312.0
80	201.5
81	131.0
82	91.5
83	60.0
84	40.0
85	30.5
86	22.5
87	16.5
88	13.0
89	11.5
90	10.5
91	8.5
92	5.0
93	2.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.4192943798213431E-4
18-19	0.0
20-21	8.042979411013564E-4
22-23	5.110136210680695E-4
24-25	6.436297080921576E-4
26-27	0.002253554200582421
28-29	0.001857128278956586
30-31	0.0029036957974412176
32-33	0.0014936900633077226
34-35	0.001145777128588944
36-37	0.007083958539068896
38-39	0.002804765586880274
40-41	6.533757045852444E-4
42-43	6.884670749473126E-5
44-45	7.450031891103182E-4
46-47	0.0037075273354784744
48-49	2.5399640483328743E-4
50-51	0.0016527861714563416
52-53	0.0034205079143297514
54-55	0.0018530792890750296
56-57	1.5874176619687217E-4
58-59	0.001990421790709717
60-61	3.8956038759527185E-4
62-63	0.0014547656481921638
64-65	0.0036930006359922056
66-67	0.004250956800882051
68-69	0.0019817760405088612
70-71	0.0016284691266336584
72-73	0.001637049994113586
74-75	0.024912202114105875
76-77	0.0088879306898462
78-79	0.0023142461861343387
80-81	0.005425063500123896
82-83	0.010901334744560667
84-85	0.0047964443029228305
86-87	0.007017183694714049
88-89	0.009932887051822123
90-91	3.288511794510632E-4
92-93	4.0187994077897194E-5
94-95	4.2373286786505615E-4
96-97	0.00339923351498163
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	139.0
20-21	389.0
22-23	826.0
24-25	1815.0
26-27	2926.0
28-29	6165.0
30-31	12750.0
32-33	17269.0
34-35	24483.0
36-37	37066.0
38-39	42409.0
40-41	43123.0
42-43	47571.0
44-45	56720.0
46-47	56092.0
48-49	52070.0
50-51	47158.0
52-53	47116.0
54-55	50340.0
56-57	52515.0
58-59	51934.0
60-61	50253.0
62-63	48455.0
64-65	51608.0
66-67	54221.0
68-69	55151.0
70-71	54318.0
72-73	51670.0
74-75	52482.0
76-77	53077.0
78-79	55919.0
80-81	56407.0
82-83	54073.0
84-85	55446.0
86-87	58750.0
88-89	63377.0
90-91	68390.0
92-93	72224.0
94-95	85508.0
96-97	196813.0
98-99	344611.0
100-101	3050687.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.62773667349465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.92927926981763	81.47483955709546
2	5.967724044178939	10.578117502551471
3	1.254244227265972	3.3348248129513807
4	0.40598992530741135	1.4392787276894805
5	0.18261454159205165	0.8092356752485645
6	0.08352890737114177	0.44417868042685665
7	0.05139262714006425	0.31883685575900955
8	0.035571010692423084	0.25220625350865084
9	0.018728553577829123	0.1493882383294148
>10	0.06859624478547216	0.9529900809697569
>50	0.0015145765079442395	0.08919507048749795
>100	8.160717631136723E-4	0.15690854498240736
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8923925064284575E-5
2	0.0	5.677177519285372E-5	0.0	0.0	1.8923925064284575E-5
3	0.0	5.677177519285372E-5	0.0	1.8923925064284575E-5	1.8923925064284575E-5
4	0.0	9.461962532142287E-5	0.0	1.8923925064284575E-5	1.8923925064284575E-5
5	0.0	9.461962532142287E-5	0.0	1.8923925064284575E-5	1.8923925064284575E-5
6	9.461962532142287E-5	9.461962532142287E-5	0.0	1.8923925064284575E-5	1.8923925064284575E-5
7	9.461962532142287E-5	9.461962532142287E-5	0.0	1.8923925064284575E-5	1.8923925064284575E-5
8	9.461962532142287E-5	9.461962532142287E-5	0.0	1.8923925064284575E-5	1.8923925064284575E-5
9	9.461962532142287E-5	2.4601102583569944E-4	0.0	1.8923925064284575E-5	1.8923925064284575E-5
10-11	1.0408158785356515E-4	2.4601102583569944E-4	0.0	2.8385887596426862E-5	1.8923925064284575E-5
12-13	1.32467475449992E-4	2.4601102583569944E-4	0.0	5.677177519285372E-5	2.8385887596426862E-5
14-15	1.32467475449992E-4	3.027828010285532E-4	0.0	5.677177519285372E-5	6.623373772499602E-5
16-17	2.4601102583569944E-4	3.027828010285532E-4	0.0	7.56957002571383E-5	7.56957002571383E-5
18-19	2.4601102583569944E-4	3.2170672609283776E-4	0.0	7.56957002571383E-5	1.1354355038570745E-4
20-21	2.4601102583569944E-4	3.4063065115712233E-4	0.0	7.56957002571383E-5	1.7031532557856117E-4
22-23	2.4601102583569944E-4	3.879404638178338E-4	0.0	7.56957002571383E-5	1.7031532557856117E-4
24-25	2.4601102583569944E-4	4.3525027647854517E-4	0.0	9.461962532142287E-5	1.8923925064284573E-4
26-27	2.4601102583569944E-4	4.5417420154282974E-4	0.0	1.513914005142766E-4	2.1762513823927259E-4
28-29	2.4601102583569944E-4	4.5417420154282974E-4	0.0	5.771797144606795E-4	2.5547298836784175E-4
30-31	2.8385887596426863E-4	5.487938268642526E-4	0.0	0.0016936912932534692	2.933208384964109E-4
32-33	3.3116868862498E-4	5.677177519285373E-4	0.0	0.00413487762654618	3.027828010285532E-4
34-35	3.9740242634997604E-4	6.717993397821025E-4	0.0	0.007815581051549529	3.027828010285532E-4
36-37	4.163263514142606E-4	7.758809276356676E-4	0.0	0.013606302121220607	3.027828010285532E-4
38-39	4.3525027647854517E-4	8.137287777642367E-4	0.0	0.02259516652675578	3.122447635606955E-4
40-41	4.5417420154282974E-4	9.461962532142287E-4	0.0	0.03211390083409092	3.2170672609283776E-4
42-43	4.5417420154282974E-4	9.745821408106555E-4	0.0	0.04074321066340469	3.2170672609283776E-4
44-45	4.5417420154282974E-4	0.0011448974663892167	0.0	0.05071611917228266	3.2170672609283776E-4
46-47	4.6363616407497205E-4	0.0013057508294356355	0.0	0.06172984355969628	3.2170672609283776E-4
48-49	4.825600891392566E-4	0.0013057508294356355	0.0	0.07329236177397415	3.2170672609283776E-4
50-51	5.014840142035412E-4	0.0015044520426106237	0.0	0.08388975980997351	3.2170672609283776E-4
52-53	5.109459767356835E-4	0.0015139140051427659	0.0	0.09382482046872291	3.595545762214069E-4
54-55	5.109459767356835E-4	0.0015139140051427659	0.0	0.10426136514167586	3.595545762214069E-4
56-57	5.109459767356835E-4	0.0015517618552713351	0.0	0.11373278963635028	3.7847850128569147E-4
58-59	5.109459767356835E-4	0.0015517618552713351	0.0	0.12203093077703907	3.9740242634997604E-4
60-61	5.582557893963949E-4	0.0015990716679320466	0.0	0.13054669705596714	3.9740242634997604E-4
62-63	6.6233737724996E-4	0.001684229330721327	0.0	0.13820142474447022	3.9740242634997604E-4
64-65	6.6233737724996E-4	0.0018734685813641728	0.0	0.14610216345880905	3.9740242634997604E-4
66-67	6.6233737724996E-4	0.0018829305438963152	0.0	0.15424891319898357	4.068643888821183E-4
68-69	6.6233737724996E-4	0.0019207783940248843	0.0	0.16216857583838667	4.163263514142606E-4
70-71	6.6233737724996E-4	0.001949164281621311	0.0	0.1689338790488684	4.163263514142606E-4
72-73	7.191091524428138E-4	0.0019586262441534533	0.0	0.17443127928004304	4.163263514142606E-4
74-75	7.191091524428138E-4	0.00198701213174988	0.0	0.17832960784328566	4.257883139464029E-4
76-77	7.191091524428138E-4	0.002034321944410592	0.0	0.18214277874373902	4.5417420154282974E-4
78-79	7.380330775070983E-4	0.0020627078320070184	0.0	0.18469750862741743	4.6363616407497205E-4
80-81	7.380330775070983E-4	0.0020721697945391606	0.0	0.1854733895550531	4.825600891392566E-4
82-83	7.380330775070983E-4	0.0020910937196034454	0.0	0.1859654116067245	4.920220516713989E-4
84-85	7.380330775070983E-4	0.0021005556821355876	0.0	0.18623980852015665	4.920220516713989E-4
86-87	7.664189651035252E-4	0.002119479607199872	0.0	0.18629658029534948	4.920220516713989E-4
88	7.758809276356676E-4	0.002176251382392726	0.0	0.18630604225788164	4.920220516713989E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6220	0.0	44.818817	1
GTATCAA	10565	0.0	34.16105	1
TCAACGC	12395	0.0	28.314264	4
ATCAACG	12580	0.0	27.996809	3
CAACGCA	12835	0.0	27.375938	5
TATCAAC	12935	0.0	27.29258	2
AACGCAG	13225	0.0	26.788208	6
ACGCAGA	15640	0.0	22.651793	7
CGCAGAG	15840	0.0	22.313406	8
GTGGTAT	3245	0.0	21.604961	1
GTACATG	15415	0.0	20.77568	1
TACATGG	15480	0.0	20.152473	2
TGGTATC	3240	0.0	19.33363	2
ACATGGG	15705	0.0	19.256222	3
GCAGAGT	18625	0.0	19.043697	9
GAGTACT	10310	0.0	18.02606	12-13
CATGGGG	11690	0.0	17.530483	4
CAGAGTA	17960	0.0	17.254236	10-11
AGAGTAC	16485	0.0	17.061697	10-11
GTACTTT	11335	0.0	16.341415	14-15
>>END_MODULE