FastQCFastQC Report
Fri 27 May 2016
SRR2138699_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138699_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9187780
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112950.050.391531
GTATCAA205250.036.1411361
TCAACGC250150.029.0681614
ATCAACG255600.028.4642873
TATCAAC260200.028.1644842
CAACGCA259000.028.0749035
AACGCAG266350.027.4530266
ACGCAGA315450.023.154137
CGCAGAG325100.022.4918928
GTACATG353950.022.4645081
TACATGG349500.022.4127162
ACATGGG358650.021.364143
GCAGAGT372550.019.4742059
GTGGTAT63200.019.1971071
TGGTATC57150.019.0921652
CATGGGG260350.019.031354
GAGTACT195850.018.18951412-13
CAGAGTA361950.017.69362310-11
GAGTACA251700.017.275261
AGAGTAC335800.016.92549310-11
Produced by FastQC (version 0.11.5)