##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138699_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9187780
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15634962961673	33.0	31.0	34.0	30.0	34.0
2	32.41202662667151	34.0	31.0	34.0	31.0	34.0
3	32.431249986394974	34.0	31.0	34.0	30.0	34.0
4	36.072000744467104	37.0	35.0	37.0	35.0	37.0
5	35.822948198585514	37.0	35.0	37.0	35.0	37.0
6	35.91698288378694	37.0	35.0	37.0	35.0	37.0
7	35.87453770116394	37.0	35.0	37.0	35.0	37.0
8	35.88752810798691	37.0	35.0	37.0	35.0	37.0
9	37.67053368713661	39.0	37.0	39.0	35.0	39.0
10-11	37.582184923888036	39.0	37.0	39.0	35.0	39.0
12-13	37.54093649390822	39.0	37.0	39.0	35.0	39.0
14-15	38.822191922314204	40.0	38.0	41.0	35.0	41.0
16-17	38.77728809353293	40.0	38.0	41.0	35.0	41.0
18-19	38.783505754382446	40.0	38.0	41.0	34.5	41.0
20-21	38.79920177080449	40.0	38.0	41.0	34.5	41.0
22-23	38.72304880356111	40.0	38.0	41.0	34.0	41.0
24-25	38.648373008845994	40.0	38.0	41.0	34.0	41.0
26-27	38.48793784563796	40.0	38.0	41.0	34.0	41.0
28-29	38.361467834123616	40.0	38.0	41.0	34.0	41.0
30-31	38.22798199841975	40.0	38.0	41.0	34.0	41.0
32-33	38.067613310546456	40.0	37.0	41.0	33.0	41.0
34-35	38.02066921634015	40.0	37.0	41.0	33.0	41.0
36-37	38.028637854953146	40.0	37.0	41.0	33.0	41.0
38-39	37.9872641448424	40.0	37.0	41.0	33.0	41.0
40-41	38.02654384821983	40.0	37.0	41.0	33.0	41.0
42-43	38.089835029600195	40.0	37.0	41.0	33.0	41.0
44-45	38.06589876180508	40.0	37.0	41.0	33.0	41.0
46-47	37.964329401950266	40.0	37.0	41.0	33.0	41.0
48-49	37.87352309209949	40.0	37.0	41.0	33.0	41.0
50-51	37.77026829022168	40.0	37.0	41.0	33.0	41.0
52-53	37.59673291190252	39.5	36.0	41.0	32.0	41.0
54-55	37.45727983107767	39.0	36.0	41.0	32.0	41.0
56-57	37.286726040597294	39.0	35.5	41.0	32.0	41.0
58-59	37.04832934911987	39.0	35.0	41.0	31.0	41.0
60-61	36.84710311925875	38.5	35.0	40.0	31.0	41.0
62-63	36.605496577700194	38.0	35.0	40.0	31.0	41.0
64-65	36.29077411847119	37.5	35.0	40.0	31.0	41.0
66-67	35.95387343822297	37.0	34.5	40.0	30.0	41.0
68-69	35.68831264206945	36.5	34.0	39.0	30.0	41.0
70-71	35.30131802739465	36.0	34.0	39.0	30.0	40.5
72-73	35.019664608248135	35.5	34.0	38.5	30.0	40.0
74-75	34.705246306727474	35.0	34.0	37.5	29.0	40.0
76-77	33.34152967742767	34.5	31.5	36.0	28.0	39.0
78-79	34.11625168651036	35.0	33.0	36.0	29.0	39.0
80-81	34.090905300779184	35.0	34.0	36.0	29.0	38.5
82-83	33.87445795996008	35.0	33.5	36.0	29.0	37.0
84-85	33.69680857749487	35.0	33.0	35.5	29.0	37.0
86-87	33.493291823416314	35.0	33.0	35.0	29.0	36.5
88-89	33.40521726649695	35.0	33.0	35.0	29.0	36.0
90-91	33.32368177698817	35.0	33.0	35.0	29.0	36.0
92-93	33.16614143157158	35.0	33.0	35.0	29.0	36.0
94-95	33.196429234478416	35.0	33.0	35.0	29.0	35.0
96-97	33.25212835020112	35.0	33.0	35.0	29.0	35.0
98-99	33.34542514638605	35.0	33.0	35.0	29.5	35.0
100	32.18460582640488	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	2.0
19	16.0
20	47.0
21	307.0
22	1325.0
23	4468.0
24	11802.0
25	25600.0
26	46562.0
27	74454.0
28	108019.0
29	144010.0
30	184986.0
31	231296.0
32	290236.0
33	370462.0
34	501468.0
35	703539.0
36	1112776.0
37	1900037.0
38	2208971.0
39	1214980.0
40	52415.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.35713306152302	19.341396942460527	13.073811083852682	24.227658912163765
2	16.016578542368233	20.801053137972396	39.21343349536014	23.968934824299232
3	19.25321459590891	25.01993952837356	29.600491087074353	26.126354788643173
4	13.186809000650864	16.71632320321122	37.25359118307143	32.843276613066486
5	13.856285196206267	37.61909841115047	34.63455807605319	13.890058316590078
6	31.914564780610768	36.280287512326154	17.824153386345778	13.980994320717299
7	28.271965589075926	31.900981521107386	21.786253044805164	18.040799845011527
8	26.180633406546523	35.39126970824291	20.300268399983455	18.12782848522712
9	26.51168182085335	15.870678226949275	20.412362942952488	37.20527700924489
10-11	25.52803832917201	26.02935638424081	27.74080354557902	20.701801741008165
12-13	25.883564909042228	23.483583629560133	28.142407632746973	22.490443828650662
14-15	22.94382320865323	24.865598653864154	25.77055066621099	26.420027471271624
16-17	22.68182421008975	27.993043142086133	26.41200635388234	22.913126293941776
18-19	22.666607167346193	27.185059938309365	28.153710689633403	21.99462220471104
20-21	23.58715917574684	26.326629639558767	28.011766866148065	22.074444318546327
22-23	23.281967563594627	26.17773229058219	27.823479056368562	22.71682108945462
24-25	22.913918252039057	26.346688915838996	28.011413625066638	22.727979207055306
26-27	22.862009308500355	26.523627271777645	27.93913300825073	22.67523041147127
28-29	22.826227006404142	26.626510423763456	27.91638097833492	22.630881591497477
30-31	23.1222889555312	26.502561402294184	27.70592308378575	22.669226558388868
32-33	22.834255830349992	26.731655360773228	27.668760823881332	22.765327984995448
34-35	22.977178103824066	26.735049145209857	27.726387975534067	22.561384775432014
36-37	23.066890697466256	26.59267609149459	27.622174402135506	22.718258808903652
38-39	22.759534837811632	26.84196538875231	27.781024236399176	22.617475537036885
40-41	23.12897735975389	26.672916895632987	27.563949131480868	22.63415661313225
42-43	22.88583120419944	26.75250902144039	27.412970052174444	22.948689722185716
44-45	22.939438507189877	26.884952919661213	27.28957974849397	22.886028824654936
46-47	23.078672833014657	26.737425379642016	27.087677568565127	23.0962242187782
48-49	22.97653764236598	26.94354578418071	26.9717697022796	23.108146871173705
50-51	22.958167408096024	26.989712705043058	27.00003862503948	23.052081261821446
52-53	23.178025120971583	26.972891179240506	26.74412677489233	23.104956924895582
54-55	23.126618005604275	26.77207545563478	26.923179014354165	23.178127524406776
56-57	23.086554803200656	26.949984069782097	26.848618963120852	23.114842163896398
58-59	23.030072337786358	27.055186472557313	26.839284053073932	23.0754571365824
60-61	23.136068197149317	26.945965517118676	26.905571379117	23.012394906615004
62-63	23.148066579559277	27.02107211584403	26.79316649941233	23.03769480518436
64-65	23.03535242892131	27.012613086473074	26.872521982619812	23.079512501985803
66-67	23.24688428291367	26.999152040706274	26.608657506540002	23.145306169840058
68-69	23.085530891443803	26.941915277688643	26.81190605036295	23.160647780504608
70-71	23.08949959972737	26.688846246020642	26.88270397332196	23.338950180930027
72-73	23.178335992185037	26.807687535600692	26.770527292674224	23.243449179540047
74-75	22.878492115821615	26.99243542494792	26.796882115266214	23.332190343964253
76-77	23.089635264252788	26.80463296925296	26.718175795496464	23.387555970997788
78-79	23.02148615868325	26.84302491848231	26.708547832555325	23.42694109027911
80-81	23.14557407675567	26.872005327455824	26.60189348293254	23.38052711285597
82-83	23.149257292443718	26.81688678530527	26.76713081040602	23.266725111844995
84-85	23.260981357988985	26.778255442444767	26.676100816527182	23.28466238303907
86-87	22.980117838690305	26.856730638251335	26.813409283202326	23.34974223985603
88-89	23.11123752570302	26.71560754485811	26.829099524859718	23.34405540457915
90-91	23.14736516349887	26.737141652748054	26.84680048062994	23.26869270312314
92-93	23.268550173397383	26.75451289253407	26.712667174739092	23.264269759329455
94-95	23.116607723385517	26.740905419283358	26.876766139542795	23.265720717788337
96-97	23.001650347606866	26.786342145298093	27.041340716250005	23.170666790845036
98-99	24.220362331292836	28.826052620953163	27.083630299257138	19.86995474849686
100	31.526108010114022	40.40544994817215	28.06844204171383	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1997.0
1	1855.5
2	2615.0
3	3736.5
4	4864.5
5	7569.5
6	10173.5
7	12664.0
8	14815.5
9	15146.5
10	13399.5
11	11319.0
12	11791.5
13	15136.5
14	23597.0
15	34970.5
16	43947.0
17	48683.0
18	49134.5
19	46581.5
20	42584.5
21	38257.5
22	36002.5
23	38075.5
24	44071.0
25	54169.5
26	68997.5
27	84883.0
28	99248.5
29	116446.5
30	132869.5
31	146763.5
32	164276.5
33	184442.0
34	204716.0
35	224933.0
36	247562.0
37	266085.0
38	278584.5
39	289688.0
40	303109.5
41	315853.5
42	325622.5
43	339842.5
44	355307.5
45	368037.5
46	377507.0
47	387692.0
48	398862.0
49	407300.0
50	411480.5
51	410729.5
52	404098.0
53	394070.5
54	382964.5
55	367147.5
56	345078.0
57	319056.5
58	290806.5
59	259127.5
60	227196.5
61	193009.0
62	158350.5
63	126869.0
64	99779.5
65	79667.0
66	62756.0
67	46922.0
68	34261.0
69	25519.5
70	17962.0
71	11935.5
72	8108.0
73	5528.0
74	3604.0
75	2364.5
76	1604.5
77	1049.0
78	695.0
79	471.0
80	326.5
81	215.5
82	149.5
83	107.5
84	68.5
85	47.0
86	37.0
87	29.5
88	21.5
89	13.5
90	9.0
91	6.0
92	3.5
93	2.5
94	3.0
95	3.0
96	3.0
97	2.5
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.2516625343662996E-4
18-19	0.0
20-21	9.142949691898921E-4
22-23	5.660467728802636E-4
24-25	7.077120415516249E-4
26-27	0.0023309375869676628
28-29	0.0020274830781466103
30-31	0.002374999542744341
32-33	0.0013306779645424155
34-35	0.0011106760047026683
36-37	0.006781981745958692
38-39	0.003197069007479133
40-41	8.449736433004603E-4
42-43	2.8445334467354E-5
44-45	6.96317916893018E-4
46-47	0.0033585576953856977
48-49	2.3628171963945298E-4
50-51	0.0013393023167658049
52-53	0.002962790077555565
54-55	0.0016574267672710445
56-57	1.1765744764831866E-4
58-59	0.0020010312524699246
60-61	3.7490092925231854E-4
62-63	0.0013963584772665762
64-65	0.0037140780237873016
66-67	0.004299382870149127
68-69	0.00200968642058905
70-71	0.0017812239551234902
72-73	0.0015862509833894003
74-75	0.024812240294938375
76-77	0.00901031738098027
78-79	0.002333291876593921
80-81	0.005611923559601409
82-83	0.011344740571580147
84-85	0.005353618589334338
86-87	0.007658352749713138
88-89	0.009448962289191502
90-91	4.2981427406836565E-4
92-93	1.6244453026930462E-5
94-95	4.732932650451418E-4
96-97	0.0036226872153915677
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	239.0
20-21	734.0
22-23	1462.0
24-25	3426.0
26-27	5468.0
28-29	12584.0
30-31	25595.0
32-33	33971.0
34-35	48593.0
36-37	72929.0
38-39	85117.0
40-41	86445.0
42-43	95369.0
44-45	111908.0
46-47	112648.0
48-49	104753.0
50-51	94682.0
52-53	92009.0
54-55	99375.0
56-57	103966.0
58-59	102366.0
60-61	100031.0
62-63	95907.0
64-65	100842.0
66-67	106437.0
68-69	109244.0
70-71	106731.0
72-73	100310.0
74-75	102068.0
76-77	102434.0
78-79	108946.0
80-81	109316.0
82-83	104117.0
84-85	104646.0
86-87	111049.0
88-89	118904.0
90-91	125434.0
92-93	130990.0
94-95	149854.0
96-97	330026.0
98-99	1887719.0
100-101	3689136.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.92474063913788
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.69526568192346	75.27651908913991
2	6.911851620317513	11.601507091427045
3	1.8222861163871382	4.588046690642772
4	0.7041565158817987	2.3638461185895574
5	0.3248346885186982	1.363083349226344
6	0.17418155135923755	0.8770884913168011
7	0.09722744835328406	0.5711851870238177
8	0.07155433352931762	0.48041431064434786
9	0.042858381495697234	0.3237190696115655
>10	0.15046745180932122	1.9948992531413166
>50	0.0038002798438495104	0.2199912780283609
>100	0.001354803705264807	0.1904499845742496
>500	9.207252730860525E-5	0.05872643449539973
>1k	6.90543481105887E-5	0.0905236521385409
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.530413222780693E-5	0.0	0.0	0.0
3	0.0	6.530413222780693E-5	0.0	0.0	0.0
4	0.0	2.0679641872138862E-4	0.0	0.0	1.0884022037967823E-5
5	1.0884022037967823E-5	2.2856446279732428E-4	0.0	0.0	1.0884022037967823E-5
6	1.0884022037967823E-5	2.2856446279732428E-4	0.0	0.0	2.1768044075935646E-5
7	1.0884022037967823E-5	2.394484848352921E-4	0.0	0.0	3.265206611390347E-5
8	1.0884022037967823E-5	2.7210055094919555E-4	0.0	0.0	3.265206611390347E-5
9	1.0884022037967823E-5	4.027088154048094E-4	0.0	0.0	4.353608815187129E-5
10-11	3.265206611390347E-5	4.027088154048094E-4	0.0	0.0	4.353608815187129E-5
12-13	3.265206611390347E-5	4.027088154048094E-4	0.0	0.0	4.89780991708552E-5
14-15	3.265206611390347E-5	6.09505234126198E-4	0.0	0.0	7.074614324679084E-5
16-17	4.353608815187129E-5	6.203892561641659E-4	0.0	0.0	9.79561983417104E-5
18-19	5.442011018983911E-5	7.074614324679084E-4	0.0	0.0	1.0884022037967822E-4
20-21	6.530413222780693E-5	8.163016528475867E-4	0.0	1.0884022037967823E-5	1.0884022037967822E-4
22-23	7.618815426577475E-5	9.196998622082811E-4	0.0	3.8094077132887376E-5	1.1428223139866214E-4
24-25	1.1428223139866213E-4	0.001044866115644911	0.0	9.251418732272649E-5	1.1972424241764605E-4
26-27	1.523763085315495E-4	0.0010775181817588143	0.0	1.959123966834208E-4	1.1972424241764605E-4
28-29	1.523763085315495E-4	0.0010775181817588143	0.0	8.924898071133615E-4	1.1972424241764605E-4
30-31	1.523763085315495E-4	0.0012625465564042673	0.0	0.002623049311150245	1.2516625343662996E-4
32-33	1.523763085315495E-4	0.0012788725894612191	0.0	0.005692343525857171	1.3060826445561387E-4
34-35	1.523763085315495E-4	0.0015455311293914309	0.0	0.010094930440215154	1.3060826445561387E-4
36-37	1.523763085315495E-4	0.0018393997244165621	0.0	0.016173656748420185	1.3060826445561387E-4
38-39	1.523763085315495E-4	0.001893819834606401	0.0	0.025952950549534275	1.3060826445561387E-4
40-41	1.7414435260748517E-4	0.0020135440770240472	0.0	0.0367009223120275	1.3060826445561387E-4
42-43	1.8502837464545298E-4	0.00207340619823287	0.0	0.04731828581006511	1.3060826445561387E-4
44-45	1.8502837464545298E-4	0.0023455067491820656	0.0	0.05918731184246902	1.3060826445561387E-4
46-47	2.0679641872138862E-4	0.002568629200960406	0.0	0.07141551060212586	1.3605027547459778E-4
48-49	2.0679641872138862E-4	0.002579513222998374	0.0	0.08355119517446	1.4149228649358169E-4
50-51	2.0679641872138862E-4	0.0028679398070045213	0.0	0.09607326252914197	1.4149228649358169E-4
52-53	2.0679641872138862E-4	0.002895149862099441	0.0	0.10817629503536219	1.5781831955053344E-4
54-55	2.1223842974037253E-4	0.002906033884137409	0.0	0.11997457492451931	1.7414435260748517E-4
56-57	2.1768044075935644E-4	0.002927801928213344	0.0	0.13068989462089864	1.7414435260748517E-4
58-59	2.2312245177834035E-4	0.002927801928213344	0.0	0.1411167877332718	1.7414435260748517E-4
60-61	2.2856446279732428E-4	0.002938685950251312	0.0	0.1513314424159046	1.7414435260748517E-4
62-63	2.394484848352921E-4	0.0029767800273841996	0.0	0.16101822202969596	1.7414435260748517E-4
64-65	2.394484848352921E-4	0.0032869746554662826	0.0	0.16969278759395634	1.7414435260748517E-4
66-67	2.503325068732599E-4	0.0033359527546371376	0.0	0.17878094599565947	1.7414435260748517E-4
68-69	2.6665853993021167E-4	0.003417582919921896	0.0	0.1877657061880019	1.7414435260748517E-4
70-71	2.7210055094919555E-4	0.003493771074187671	0.0	0.1951069790526112	2.013544077024047E-4
72-73	2.7210055094919555E-4	0.0035046550962256388	0.0	0.20098979296413277	2.2856446279732428E-4
74-75	2.7210055094919555E-4	0.0035590752064154777	0.0	0.2051801414487504	2.2856446279732428E-4
76-77	2.7210055094919555E-4	0.003722335536984995	0.0	0.20945212009865277	2.2856446279732428E-4
78-79	2.7210055094919555E-4	0.0037767556471748343	0.0	0.21222754571833458	2.2856446279732428E-4
80-81	2.938685950251312E-4	0.003782197658193818	0.0	0.21320166569073268	2.340064738163082E-4
82-83	2.938685950251312E-4	0.003793081680231786	0.0	0.21380028690282094	2.394484848352921E-4
84-85	2.9931060604411507E-4	0.0038202917353267057	0.0	0.21401252533256132	2.394484848352921E-4
86-87	3.04752617063099E-4	0.0038964798895924804	0.0	0.21412136555294098	2.4489049585427604E-4
88	3.04752617063099E-4	0.004005320109972159	0.0	0.21413224957497895	2.503325068732599E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11295	0.0	50.39153	1
GTATCAA	20525	0.0	36.141136	1
TCAACGC	25015	0.0	29.068161	4
ATCAACG	25560	0.0	28.464287	3
TATCAAC	26020	0.0	28.164484	2
CAACGCA	25900	0.0	28.074903	5
AACGCAG	26635	0.0	27.453026	6
ACGCAGA	31545	0.0	23.15413	7
CGCAGAG	32510	0.0	22.491892	8
GTACATG	35395	0.0	22.464508	1
TACATGG	34950	0.0	22.412716	2
ACATGGG	35865	0.0	21.36414	3
GCAGAGT	37255	0.0	19.474205	9
GTGGTAT	6320	0.0	19.197107	1
TGGTATC	5715	0.0	19.092165	2
CATGGGG	26035	0.0	19.03135	4
GAGTACT	19585	0.0	18.189514	12-13
CAGAGTA	36195	0.0	17.693623	10-11
GAGTACA	25170	0.0	17.27526	1
AGAGTAC	33580	0.0	16.925493	10-11
>>END_MODULE