FastQCFastQC Report
Fri 27 May 2016
SRR2138698_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138698_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8970998
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99000.044.0781821
GTATCAA170200.033.7554931
TCAACGC201700.027.8190564
ATCAACG206550.027.2051773
CAACGCA208850.026.8861245
TATCAAC214500.026.4620632
AACGCAG213500.026.3386066
ACGCAGA248750.022.6062017
CGCAGAG256550.021.8238728
GTACATG268450.021.340781
TACATGG266400.020.9254362
ACATGGG266300.020.4908263
GCAGAGT303950.018.3536729
CATGGGG198750.018.337494
GAGTACT162850.017.739312-13
AGAGTAC269400.016.72586410-11
CAGAGTA293550.016.67859810-11
GTGGTAT58000.015.761921
GTACTTT184300.015.73067814-15
GAGTACA192800.015.6790761
Produced by FastQC (version 0.11.5)