FastQCFastQC Report
Fri 27 May 2016
SRR2138697_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138697_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7256646
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83550.047.489251
GTATCAA146850.033.303021
TCAACGC174900.027.3111524
ATCAACG180000.026.5825063
CAACGCA183050.026.0951655
TATCAAC186800.025.897752
AACGCAG187200.025.6686826
GTACATG255400.023.6531981
TACATGG254950.023.1049652
ACATGGG258650.022.412953
ACGCAGA222600.021.5866017
TAGCGCG3804.6367404E-621.37963794
CGCAGAG228350.021.0964458
CATGGGG190400.020.155664
GTGGTAT48000.019.1407591
TGGTATC46750.018.435132
GCAGAGT269900.017.6679889
GAGTACT135450.017.27262312-13
CAGAGTA256750.016.7282710-11
ATGGGGG121200.016.6368475
Produced by FastQC (version 0.11.5)