##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138697_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7256646
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17915769902514	33.0	31.0	34.0	30.0	34.0
2	32.435571199146274	34.0	31.0	34.0	31.0	34.0
3	32.45739767380137	34.0	31.0	34.0	31.0	34.0
4	36.08665821648183	37.0	35.0	37.0	35.0	37.0
5	35.8385033526508	37.0	35.0	37.0	35.0	37.0
6	35.93452402115247	37.0	35.0	37.0	35.0	37.0
7	35.89461646606435	37.0	35.0	37.0	35.0	37.0
8	35.90709702526484	37.0	35.0	37.0	35.0	37.0
9	37.697346956155776	39.0	37.0	39.0	35.0	39.0
10-11	37.60902550572261	39.0	37.0	39.0	35.0	39.0
12-13	37.56936303631181	39.0	37.0	39.0	35.0	39.0
14-15	38.8668796851879	40.0	38.0	41.0	35.0	41.0
16-17	38.82048056361024	40.0	38.0	41.0	35.0	41.0
18-19	38.81996758557604	40.0	38.0	41.0	35.0	41.0
20-21	38.83250040069625	40.0	38.0	41.0	35.0	41.0
22-23	38.75863304024987	40.0	38.0	41.0	34.0	41.0
24-25	38.682710184967966	40.0	38.0	41.0	34.0	41.0
26-27	38.525837499421925	40.0	38.0	41.0	34.0	41.0
28-29	38.39783159213002	40.0	38.0	41.0	34.0	41.0
30-31	38.26838992647583	40.0	38.0	41.0	34.0	41.0
32-33	38.11518869567614	40.0	37.5	41.0	33.5	41.0
34-35	38.07732342321127	40.0	37.5	41.0	33.0	41.0
36-37	38.087491587590726	40.0	37.5	41.0	33.0	41.0
38-39	38.04943611968611	40.0	37.0	41.0	33.0	41.0
40-41	38.088210952520356	40.0	37.5	41.0	33.0	41.0
42-43	38.14944627230413	40.0	38.0	41.0	33.0	41.0
44-45	38.126990171765314	40.0	37.0	41.0	33.0	41.0
46-47	38.026727871835774	40.0	37.0	41.0	33.0	41.0
48-49	37.939896190310115	40.0	37.0	41.0	33.0	41.0
50-51	37.835097832861265	40.0	37.0	41.0	33.0	41.0
52-53	37.66156929552448	40.0	36.5	41.0	32.5	41.0
54-55	37.524876721720005	39.0	36.0	41.0	32.0	41.0
56-57	37.351406487111106	39.0	36.0	41.0	32.0	41.0
58-59	37.11263604247078	39.0	35.0	41.0	31.0	41.0
60-61	36.91044935763644	38.5	35.0	40.0	31.0	41.0
62-63	36.6643460694138	38.0	35.0	40.0	31.0	41.0
64-65	36.34670076301862	37.5	35.0	40.0	31.0	41.0
66-67	36.00774030580742	37.0	34.5	40.0	30.0	41.0
68-69	35.73745498938679	36.5	34.0	39.0	30.0	41.0
70-71	35.34329227239247	36.0	34.0	39.0	30.0	40.5
72-73	35.054378553647226	35.5	34.0	38.5	30.0	40.0
74-75	34.73365537809508	35.0	34.0	37.5	29.0	40.0
76-77	33.373228142551355	34.5	32.0	36.0	28.0	39.0
78-79	34.14101914533174	35.0	33.5	36.5	29.0	39.0
80-81	34.10651751512224	35.0	34.0	36.0	29.0	38.5
82-83	33.89135910511848	35.0	33.5	36.0	29.0	37.0
84-85	33.7099661943008	35.0	33.0	35.5	29.0	37.0
86-87	33.504090792265785	35.0	33.0	35.0	29.0	36.5
88-89	33.41381273409642	35.0	33.0	35.0	29.0	36.0
90-91	33.329707993493464	35.0	33.0	35.0	29.0	36.0
92-93	33.1693846083544	35.0	33.0	35.0	29.0	36.0
94-95	33.202220479105165	35.0	33.0	35.0	29.0	35.0
96-97	33.25872570504906	35.0	33.0	35.0	29.0	35.0
98-99	33.351352415077905	35.0	33.0	35.0	29.5	35.0
100	32.15078800503133	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	3.0
20	50.0
21	236.0
22	1000.0
23	3407.0
24	9041.0
25	19417.0
26	35765.0
27	57586.0
28	83289.0
29	111467.0
30	143895.0
31	179657.0
32	225262.0
33	287354.0
34	388074.0
35	543451.0
36	861988.0
37	1497294.0
38	1778280.0
39	987340.0
40	42787.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.406196195873406	19.121464654607653	12.921575614960409	24.550763534558527
2	16.02094135500064	20.67518520264045	39.203827773877904	24.100045668481005
3	19.464749968511626	24.603129324484065	29.3706348635444	26.561485843459913
4	13.254153502871713	16.600479064294994	36.99000888289163	33.15535854994167
5	14.020389033721639	37.37693694855723	34.56865609814782	14.034017919573314
6	32.29409289084792	35.95712123755245	17.73086354219291	14.017922329406726
7	28.500067386503353	31.57307108545739	21.660185711139828	18.266675816899433
8	26.10779139563925	35.55051465925167	20.26688362640261	18.074810318706465
9	26.400970916867106	15.825672080462516	20.22667221192821	37.54668479074217
10-11	25.645980250380134	25.97586405620448	27.636335023094695	20.741820670320696
12-13	25.903420119983807	23.340617690321398	28.069978885562286	22.685983304132513
14-15	22.945400671329427	24.813970531289524	25.535736482115844	26.704892315265205
16-17	22.783332735030022	27.892480140406743	26.300846336209855	23.023340788353384
18-19	22.7974742050253	27.215004011495118	27.82361162443366	22.16391015904593
20-21	23.57744674708193	26.371373535452157	27.726741996687704	22.324437720778207
22-23	23.32291728041441	26.216836517585833	27.624392238989664	22.83585396301009
24-25	23.0108625107594	26.340396166373775	27.77167167255481	22.877069650312016
26-27	22.918374957008314	26.511369801315666	27.706979974355306	22.86327526732072
28-29	22.887031341438345	26.663886808672192	27.67552419218226	22.7735576577072
30-31	23.21813311332152	26.524413804506253	27.492859316261125	22.7645937659111
32-33	22.91823980128636	26.705833746499643	27.504049561375805	22.87187689083819
34-35	23.07167507782582	26.718825065966676	27.47179512419864	22.737704732008865
36-37	23.13662166329103	26.60724590352721	27.43487623616384	22.821256197017924
38-39	22.8642539891104	26.784797301642744	27.578774762280272	22.77217394696659
40-41	23.20478603350843	26.605800468557632	27.402185722073973	22.787227775859968
42-43	23.0212798039281	26.690391459074732	27.26895896769908	23.01936976929809
44-45	23.064328517130225	26.81062906147284	27.128347553994097	22.996694867402834
46-47	23.129931155242613	26.75405209130438	26.948685144692035	23.167331608760968
48-49	23.0388776087807	26.883361486208386	26.934084555611133	23.14367634939978
50-51	22.994662412353264	27.01380682265816	26.89249525783442	23.099035507154156
52-53	23.24115203849006	26.897514890939483	26.69584540164065	23.165487668929803
54-55	23.14883561261067	26.764140816732585	26.788819779010126	23.29820379164662
56-57	23.164266896276466	26.944791218210025	26.77442838579941	23.1165134997141
58-59	23.09666099208233	27.004855670272796	26.808367190113735	23.090116147531134
60-61	23.18538440881462	26.898896210316988	26.826092838157606	23.089626542710786
62-63	23.156001967531807	26.959853277223207	26.759974828126566	23.12416992711842
64-65	23.136847097863843	26.95103833047433	26.737943434704004	23.17417113695782
66-67	23.192205403749302	27.003678812367504	26.53724195526243	23.266873828620763
68-69	23.143213730139646	26.969705980556164	26.694997837779972	23.192082451524215
70-71	23.133222798652927	26.693056239655693	26.786298861095048	23.387422100596332
72-73	23.20720995195977	26.734523693759808	26.71939525418227	23.338871100098153
74-75	22.923510730036973	26.982503674111374	26.748417640133248	23.345567955718398
76-77	23.128472165073255	26.856827247092102	26.6604806377204	23.354219950114235
78-79	23.087763159143694	26.84579219180453	26.649442777649192	23.417001871402586
80-81	23.122215251385402	26.8593862513486	26.63637922023933	23.382019277026668
82-83	23.133485260211035	26.78321488649842	26.74269979674647	23.340600056544073
84-85	23.262891498322624	26.76978405641364	26.662277417802215	23.30504702746152
86-87	23.028345325613873	26.81646769330716	26.801663927711	23.353523053367965
88-89	23.113303055046288	26.767350321151362	26.781807295742365	23.337539328059982
90-91	23.201348348180854	26.6941890993689	26.827837941290355	23.276624611159892
92-93	23.231430186406744	26.687490808649848	26.786254176672742	23.294824828270663
94-95	23.085458036463727	26.738936644481022	26.865362397926557	23.310242921128697
96-97	23.055259083860342	26.860346386944272	26.9572694103637	23.12712511883169
98-99	24.222100819845917	28.83644369545351	27.079927794868286	19.861527689832283
100	31.51461317285372	40.39157015800554	28.093816669140736	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1190.0
1	1071.5
2	1500.5
3	2237.0
4	3156.5
5	5152.5
6	6906.0
7	8279.0
8	9403.0
9	9478.5
10	8528.5
11	7470.5
12	7813.5
13	10209.5
14	16085.5
15	23510.5
16	29196.5
17	32556.0
18	33353.5
19	31858.0
20	29197.5
21	26470.0
22	24998.5
23	26398.5
24	30653.0
25	38065.5
26	49221.5
27	60974.5
28	71678.5
29	84170.0
30	96971.0
31	109434.5
32	124390.5
33	140749.0
34	157140.0
35	173322.5
36	191668.0
37	207869.0
38	219288.5
39	229940.5
40	243198.5
41	255597.5
42	265397.5
43	277792.0
44	290112.5
45	299415.5
46	307699.0
47	317114.0
48	324664.0
49	329601.0
50	332885.5
51	333267.5
52	328786.0
53	322209.0
54	313726.0
55	300163.5
56	282684.5
57	261636.5
58	237661.5
59	210245.5
60	181330.0
61	151637.0
62	122977.5
63	97403.5
64	74637.5
65	56564.0
66	42389.5
67	32315.0
68	24620.0
69	17957.5
70	12906.0
71	8865.5
72	5926.0
73	3860.5
74	2527.5
75	1738.5
76	1213.5
77	823.5
78	540.0
79	339.5
80	223.0
81	149.0
82	103.0
83	69.5
84	49.0
85	35.5
86	26.5
87	20.0
88	13.0
89	8.0
90	6.0
91	5.0
92	3.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.9981682997902885E-4
18-19	0.0
20-21	7.372856806993492E-4
22-23	5.926399221133319E-4
24-25	7.788612373237749E-4
26-27	0.002392583515127161
28-29	0.0023943098501907237
30-31	0.002875406825137525
32-33	0.0015045013014976234
34-35	0.0011068871493047357
36-37	0.006731210170025046
38-39	0.002819779018370613
40-41	7.493554472645743E-4
42-43	1.297380329638394E-4
44-45	5.905242295007533E-4
46-47	0.003205861437497927
48-49	2.9185510965333225E-4
50-51	0.0015680716967831122
52-53	0.003210364866012089
54-55	0.0019841988018384532
56-57	1.5698001840590717E-4
58-59	0.001908545216974951
60-61	4.350780203660021E-4
62-63	0.0016156478266354396
64-65	0.003866941516971412
66-67	0.004547740978764311
68-69	0.0021069519388970345
70-71	0.0020691415726493277
72-73	0.0015827770076192787
74-75	0.024213296176134484
76-77	0.009029096082262619
78-79	0.0022908275902163958
80-81	0.0055716204460114615
82-83	0.011309418824880759
84-85	0.004962039916599486
86-87	0.007294499335578925
88-89	0.009317605452469048
90-91	3.825714925442857E-4
92-93	5.134829848683754E-5
94-95	5.038111740910065E-4
96-97	0.003564013571763681
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	207.0
20-21	555.0
22-23	1123.0
24-25	2408.0
26-27	4133.0
28-29	9604.0
30-31	20812.0
32-33	26956.0
34-35	38565.0
36-37	59697.0
38-39	69481.0
40-41	68372.0
42-43	75183.0
44-45	87935.0
46-47	88831.0
48-49	83225.0
50-51	75920.0
52-53	73289.0
54-55	79262.0
56-57	83089.0
58-59	81871.0
60-61	79420.0
62-63	76072.0
64-65	79739.0
66-67	84348.0
68-69	85890.0
70-71	83190.0
72-73	78538.0
74-75	79556.0
76-77	79934.0
78-79	84706.0
80-81	84617.0
82-83	80450.0
84-85	80972.0
86-87	85782.0
88-89	92230.0
90-91	97345.0
92-93	102334.0
94-95	117953.0
96-97	261684.0
98-99	1494445.0
100-101	2916923.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.81699950434624
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.70006310659873	77.83607270663157
2	6.481369255549255	11.124233243819107
3	1.5460583931758034	3.9803427708257484
4	0.5693498906553497	1.9543959713667896
5	0.2655337623401929	1.1393655375567764
6	0.13299570553340892	0.684797543750444
7	0.07804833682340326	0.4688511857742331
8	0.0564157664961525	0.3873145444350106
9	0.0369505151866594	0.2853884109113
>10	0.12860268803687236	1.7615127786787779
>50	0.0034305501986622716	0.20052656850215755
>100	0.0011285562293807546	0.13901904417614022
>500	2.673662302702609E-5	0.012573623072518886
>1k	2.673655307055546E-5	0.02560607049949824
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.5121884132145896E-5	0.0	0.0	0.0
3	0.0	5.5121884132145896E-5	0.0	0.0	0.0
4	0.0	9.646329723125532E-5	0.0	0.0	0.0
5	2.7560942066072948E-5	9.646329723125532E-5	0.0	0.0	0.0
6	5.5121884132145896E-5	9.646329723125532E-5	0.0	0.0	2.7560942066072948E-5
7	5.5121884132145896E-5	9.646329723125532E-5	0.0	0.0	2.7560942066072948E-5
8	6.890235516518238E-5	1.1024376826429179E-4	0.0	0.0	4.134141309910942E-5
9	6.890235516518238E-5	1.7914612342947417E-4	0.0	6.890235516518238E-5	8.268282619821884E-5
10-11	8.268282619821884E-5	1.7914612342947417E-4	0.0	8.957306171473707E-5	8.268282619821884E-5
12-13	8.268282619821884E-5	1.7914612342947417E-4	0.0	9.646329723125532E-5	8.268282619821884E-5
14-15	8.957306171473707E-5	2.3426800756162005E-4	0.0	1.1024376826429179E-4	1.4469494584688297E-4
16-17	1.3780471033036473E-4	2.4804847859465653E-4	0.0	1.1713400378081002E-4	1.5158518136340122E-4
18-19	1.5158518136340122E-4	2.6871918514421127E-4	0.0	1.3780471033036473E-4	1.5847541687991946E-4
20-21	1.5158518136340122E-4	2.756094206607295E-4	0.0	1.653656523964377E-4	1.6536565239643768E-4
22-23	1.5158518136340122E-4	3.0317036272680244E-4	0.0	1.7914612342947417E-4	2.0670706549554712E-4
24-25	1.5158518136340122E-4	3.6518248237546653E-4	0.0	2.7560942066072946E-4	2.61828949627693E-4
26-27	1.5158518136340122E-4	3.8585318892502126E-4	0.0	3.514020113424301E-4	2.61828949627693E-4
28-29	1.5158518136340122E-4	3.8585318892502126E-4	0.0	0.0010886572116098816	2.61828949627693E-4
30-31	1.5158518136340122E-4	4.5475554409020366E-4	0.0	0.0029214598590037327	2.61828949627693E-4
32-33	1.5158518136340122E-4	4.616457796067219E-4	0.0	0.005911822073172647	2.756094206607295E-4
34-35	1.5158518136340122E-4	5.581090768379773E-4	0.0	0.01036291421684343	3.0317036272680244E-4
36-37	1.5158518136340122E-4	6.476821385527143E-4	0.0	0.017218698555779073	3.100605982433207E-4
38-39	1.5158518136340122E-4	6.752430806187872E-4	0.0	0.0270510646378506	3.3073130479287536E-4
40-41	1.5158518136340122E-4	7.303649647509332E-4	0.0	0.0372899546153967	3.3073130479287536E-4
42-43	1.5158518136340122E-4	7.510356713004878E-4	0.0	0.047742441893954865	3.3073130479287536E-4
44-45	1.653656523964377E-4	8.543892040482615E-4	0.0	0.059710780986147044	3.376215403093936E-4
46-47	1.7914612342947417E-4	9.370720302464803E-4	0.0	0.07287113082269688	3.4451177582591185E-4
48-49	1.7914612342947417E-4	9.370720302464803E-4	0.0	0.0857627614741025	3.5829224685894834E-4
50-51	1.7914612342947417E-4	0.001047315798510772	0.0	0.0987646358937724	3.5829224685894834E-4
52-53	1.7914612342947417E-4	0.0010610962695438086	0.0	0.11071919451493155	3.720727178919848E-4
54-55	1.7914612342947417E-4	0.001074876740576845	0.0	0.12261863125195856	3.8585318892502126E-4
56-57	1.7914612342947417E-4	0.001102437682642918	0.0	0.13371880066906944	3.9963365995805775E-4
58-59	1.7914612342947417E-4	0.0011231083891924726	0.0	0.1441643977121111	4.1341413099109424E-4
60-61	2.0670706549554712E-4	0.0011299986247089908	0.0	0.1536178008407741	4.203043665076125E-4
62-63	2.0670706549554712E-4	0.0011437790957420273	0.0	0.16270602148706165	4.5475554409020366E-4
64-65	2.0670706549554712E-4	0.0011989009798741733	0.0	0.17214564414469163	4.5475554409020366E-4
66-67	2.4804847859465653E-4	0.0012057912153906915	0.0	0.18149569374060687	4.616457796067219E-4
68-69	2.4804847859465653E-4	0.001219571686423728	0.0	0.1906459265065431	4.6853601512324015E-4
70-71	2.4804847859465653E-4	0.0012333521574567645	0.0	0.19821829533919666	5.305481347719043E-4
72-73	2.4804847859465653E-4	0.0012402423929732827	0.0	0.2037511544589608	6.063407254536049E-4
74-75	2.61828949627693E-4	0.0012609130995228375	0.0	0.20788529576887171	6.063407254536049E-4
76-77	2.756094206607295E-4	0.0012953642771054287	0.0	0.21176449836467148	6.063407254536049E-4
78-79	2.8938989169376595E-4	0.0013160349836549832	0.0	0.2145963851619605	6.063407254536049E-4
80-81	2.8938989169376595E-4	0.0013298154546880197	0.0	0.2153887622463601	6.270114320031596E-4
82-83	2.962801272102842E-4	0.001336705690204538	0.0	0.21586418849699984	6.545723740692326E-4
84-85	3.0317036272680244E-4	0.001336705690204538	0.0	0.2160984565045615	6.614626095857507E-4
86-87	3.0317036272680244E-4	0.0013573763967540927	0.0	0.2162362612148918	6.752430806187872E-4
88	3.0317036272680244E-4	0.001391827574336684	0.0	0.21624315145040834	6.752430806187872E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8355	0.0	47.48925	1
GTATCAA	14685	0.0	33.30302	1
TCAACGC	17490	0.0	27.311152	4
ATCAACG	18000	0.0	26.582506	3
CAACGCA	18305	0.0	26.095165	5
TATCAAC	18680	0.0	25.89775	2
AACGCAG	18720	0.0	25.668682	6
GTACATG	25540	0.0	23.653198	1
TACATGG	25495	0.0	23.104965	2
ACATGGG	25865	0.0	22.41295	3
ACGCAGA	22260	0.0	21.586601	7
TAGCGCG	380	4.6367404E-6	21.379637	94
CGCAGAG	22835	0.0	21.096445	8
CATGGGG	19040	0.0	20.15566	4
GTGGTAT	4800	0.0	19.140759	1
TGGTATC	4675	0.0	18.43513	2
GCAGAGT	26990	0.0	17.667988	9
GAGTACT	13545	0.0	17.272623	12-13
CAGAGTA	25675	0.0	16.72827	10-11
ATGGGGG	12120	0.0	16.636847	5
>>END_MODULE