FastQCFastQC Report
Fri 27 May 2016
SRR2138696_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138696_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7156639
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79000.046.046071
GTATCAA133350.032.118191
TCAACGC155200.026.8813274
ATCAACG159250.026.2493113
CAACGCA161900.025.7688835
TATCAAC165000.025.4591162
AACGCAG166950.025.112516
GTACATG209350.023.1678331
TACATGG212600.022.5623342
ACATGGG219500.021.4036623
ACGCAGA198150.021.137647
CATGGGG169550.019.8546644
CGCAGAG212500.019.7682558
GTGGTAT41750.019.0994851
GCAGAGT241600.017.3702169
GAGTACT130350.017.1698412-13
CAGAGTA231500.016.82316410-11
TGGTATC44050.016.6092682
ATGGGGG98250.016.608655
AGAGTAC212700.016.31001510-11
Produced by FastQC (version 0.11.5)