##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138696_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7156639
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1931811846315	33.0	31.0	34.0	30.0	34.0
2	32.449218830235814	34.0	31.0	34.0	31.0	34.0
3	32.472706811116225	34.0	31.0	34.0	31.0	34.0
4	36.096905265167074	37.0	35.0	37.0	35.0	37.0
5	35.848939565066786	37.0	35.0	37.0	35.0	37.0
6	35.94397621565095	37.0	35.0	37.0	35.0	37.0
7	35.904065022701296	37.0	35.0	37.0	35.0	37.0
8	35.91581062004106	37.0	35.0	37.0	35.0	37.0
9	37.70929300751372	39.0	37.0	39.0	35.0	39.0
10-11	37.621068493185135	39.0	37.0	39.0	35.0	39.0
12-13	37.5835867227619	39.0	37.0	39.0	35.0	39.0
14-15	38.88792776888704	40.0	38.0	41.0	35.0	41.0
16-17	38.84593473277051	40.0	38.0	41.0	35.0	41.0
18-19	38.842237955554275	40.0	38.0	41.0	35.0	41.0
20-21	38.85405878037351	40.0	38.0	41.0	35.0	41.0
22-23	38.779673452746316	40.0	38.0	41.0	34.0	41.0
24-25	38.70397198426445	40.0	38.0	41.0	34.0	41.0
26-27	38.548178293209716	40.0	38.0	41.0	34.0	41.0
28-29	38.42169267958113	40.0	38.0	41.0	34.0	41.0
30-31	38.29333828901032	40.0	38.0	41.0	34.0	41.0
32-33	38.139581686166274	40.0	38.0	41.0	33.5	41.0
34-35	38.0986484983422	40.0	37.5	41.0	33.0	41.0
36-37	38.11202734618004	40.0	38.0	41.0	33.0	41.0
38-39	38.071811851142556	40.0	37.0	41.0	33.0	41.0
40-41	38.10474867705908	40.0	37.5	41.0	33.0	41.0
42-43	38.164336188676124	40.0	38.0	41.0	33.0	41.0
44-45	38.143661933208364	40.0	37.5	41.0	33.0	41.0
46-47	38.04173963625658	40.0	37.0	41.0	33.0	41.0
48-49	37.95148290930756	40.0	37.0	41.0	33.0	41.0
50-51	37.84741385325171	40.0	37.0	41.0	33.0	41.0
52-53	37.675532398797614	40.0	36.5	41.0	32.5	41.0
54-55	37.544284875638795	39.0	36.0	41.0	32.0	41.0
56-57	37.376953419522394	39.0	36.0	41.0	32.0	41.0
58-59	37.142632993959396	39.0	35.0	41.0	31.5	41.0
60-61	36.94723043464081	39.0	35.0	40.5	31.0	41.0
62-63	36.710725613791396	38.0	35.0	40.0	31.0	41.0
64-65	36.40280060619207	37.5	35.0	40.0	31.0	41.0
66-67	36.07054162634951	37.0	35.0	40.0	30.5	41.0
68-69	35.812048612597415	36.5	34.0	39.0	30.0	41.0
70-71	35.42303378716629	36.0	34.0	39.0	30.0	41.0
72-73	35.138580244721766	35.5	34.0	39.0	30.0	40.0
74-75	34.82286352522281	35.0	34.0	38.0	29.5	40.0
76-77	33.454220954896364	34.5	32.0	36.0	28.0	39.0
78-79	34.21915681768398	35.0	33.5	37.0	29.0	39.0
80-81	34.18147638919319	35.0	34.0	36.0	30.0	39.0
82-83	33.95526593719529	35.0	34.0	36.0	29.0	37.5
84-85	33.766524986773874	35.0	33.5	35.5	29.0	37.0
86-87	33.552050496130285	35.0	33.0	35.0	29.0	37.0
88-89	33.455698277702275	35.0	33.0	35.0	29.0	36.0
90-91	33.36448171235164	35.0	33.0	35.0	29.0	36.0
92-93	33.1989241780266	35.0	33.0	35.0	29.0	36.0
94-95	33.22599938716549	35.0	33.0	35.0	29.0	35.5
96-97	33.28095561449153	35.0	33.0	35.0	29.0	35.0
98-99	33.37228211679739	35.0	33.0	35.0	30.0	35.0
100	32.17181756666584	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	10.0
20	30.0
21	205.0
22	928.0
23	3300.0
24	8928.0
25	18988.0
26	35564.0
27	57212.0
28	81987.0
29	110502.0
30	141395.0
31	176689.0
32	220608.0
33	280640.0
34	377330.0
35	533378.0
36	852390.0
37	1465274.0
38	1778319.0
39	971890.0
40	41068.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.776644176127924	19.55707141299149	13.299608936541302	24.36667547433928
2	15.848556843512714	20.808175457781232	39.42827072875969	23.914996969946365
3	19.178388067359553	24.70768750526609	29.66395259003563	26.449971837338726
4	13.15297585919871	16.95958116652244	37.340391208778314	32.54705176550054
5	14.050170757530175	37.18185310171437	34.870628517101395	13.897347623654063
6	32.039690698385094	36.43886466817734	17.757986675029997	13.763457958407571
7	28.087863590716257	31.776061360647084	21.984677444258402	18.151397604378257
8	25.723443644425824	35.7736641459769	20.29428898118237	18.2086032284149
9	25.941562792254857	16.113220186179575	20.69340091067888	37.251816110886686
10-11	25.255458602844154	26.149341890795384	27.961337717327922	20.633861789032533
12-13	25.616396188210693	23.570044541858266	28.233022512383254	22.58053675754778
14-15	22.611640743650753	25.041272865656627	25.7514805483412	26.595605842351418
16-17	22.638139816164767	28.20962000808761	26.40469824747025	22.747541928277375
18-19	22.619514551453552	27.412309046187744	28.006645298163008	21.961531104195696
20-21	23.401949367695313	26.57017667109795	27.920703104339722	22.107170856867018
22-23	23.118238772221826	26.481108041293012	27.67677889903	22.72387428745516
24-25	22.82730013512303	26.58654240622424	27.91743503590398	22.66872242274875
26-27	22.869420601931107	26.757758984671675	27.761611393526632	22.611209019870586
28-29	22.836733314611294	26.814894012683133	27.76272878354173	22.58564388916384
30-31	23.018359957759692	26.652574885797716	27.639377943524963	22.68968721291763
32-33	22.79374450952558	26.77451500780032	27.610353002283812	22.821387480390285
34-35	22.952172083134982	26.884720570442983	27.617474566870982	22.545632779551052
36-37	23.064437418581623	26.729488918729928	27.41781049367087	22.78826316901758
38-39	22.72186623262848	27.0458007684913	27.593861938013113	22.638471060867104
40-41	23.029004348773025	26.832516076233855	27.457097158476024	22.6813824165171
42-43	22.80828355936289	26.942637278201236	27.27692936154995	22.972149800885916
44-45	22.855365879000573	27.029451163102834	27.201522861017224	22.91366009687937
46-47	23.00251696742631	26.935782284812916	27.108977888957725	22.95272285880305
48-49	22.93337391730533	27.182946952663062	26.87760231340701	23.006076816624592
50-51	22.913321714802468	27.1836577517411	26.97321162332788	22.92980891012855
52-53	23.111256158066126	27.165776422579807	26.76742534662675	22.95554207272732
54-55	23.105015984409828	26.99184648738468	26.903236243536018	22.999901284669477
56-57	23.097584947572773	27.156184857801723	26.78040344677371	22.965826747851793
58-59	22.97058110723841	27.215983558201607	26.801972952029875	23.01146238253011
60-61	23.037882029902075	27.186701431718557	26.884064425721494	22.891352112657874
62-63	23.107352370554736	27.239427685172323	26.753329984725582	22.89988995954736
64-65	22.97374279327446	27.22134627727803	26.822075596416102	22.982835333031403
66-67	23.131930366083754	27.195389254742725	26.65369845075627	23.018981928417254
68-69	23.023411153564606	27.132773937038852	26.804598807705222	23.039216101691324
70-71	23.03694504881508	27.033419069066788	26.764933337697	23.164702544421132
72-73	23.057167904477147	27.083617660698344	26.699854053022758	23.15936038180175
74-75	22.88585261033361	27.165398699387787	26.772786506902264	23.17596218337634
76-77	23.012196786099544	27.116117768248106	26.672707136383934	23.198978309268416
78-79	22.865197905842642	27.150311686641004	26.735094892071537	23.249395515444817
80-81	23.0245976179416	27.14513842766351	26.674864708413747	23.155399245981144
82-83	23.04428869905386	27.142177369307273	26.812399261410448	23.00113467022842
84-85	23.08746889329874	27.115686179153787	26.70394519354513	23.09289973400234
86-87	22.926303108113117	27.1986741063308	26.785047834781523	23.089974950774554
88-89	23.019197597745553	27.124477183570217	26.74555757927538	23.11076763940885
90-91	22.912396980132783	27.146919783299932	26.80121729696086	23.13946593960643
92-93	23.007284460162282	27.186486267205325	26.812521105470395	22.993708167162
94-95	22.877373175829455	27.28703153846527	26.86132333532934	22.974271950375933
96-97	22.78772297576145	27.33986621719814	27.015349572511422	22.857061234528988
98-99	24.04133234111691	29.36115351343921	27.065534712190527	19.531979433253355
100	30.976956417645397	41.00149483848166	28.021548743872938	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1723.0
1	1479.5
2	1752.5
3	2385.0
4	3157.0
5	5022.5
6	6735.0
7	7995.5
8	8834.5
9	8784.0
10	8002.0
11	7193.0
12	7666.0
13	9846.0
14	15364.5
15	22876.5
16	28954.5
17	32592.5
18	33717.5
19	33011.5
20	31068.5
21	28701.0
22	27681.0
23	29696.0
24	34440.0
25	41766.5
26	52318.5
27	64236.5
28	76802.5
29	91112.0
30	105351.0
31	118840.5
32	132724.0
33	146684.5
34	160725.0
35	174802.0
36	190160.5
37	205456.5
38	218548.5
39	230270.0
40	241434.5
41	252881.5
42	263028.5
43	272235.5
44	282047.5
45	292034.0
46	301985.5
47	312270.0
48	319998.5
49	322187.5
50	318944.5
51	314461.0
52	305588.0
53	291946.5
54	279431.0
55	266227.0
56	249744.5
57	229369.5
58	208909.0
59	186520.0
60	162251.0
61	139338.5
62	117144.0
63	96015.5
64	77985.5
65	65516.0
66	55440.0
67	41258.5
68	28687.0
69	21886.5
70	15545.5
71	10554.0
72	7513.5
73	5277.0
74	3648.0
75	2452.5
76	1621.0
77	1049.5
78	687.0
79	460.5
80	313.5
81	199.0
82	134.5
83	95.0
84	67.5
85	46.5
86	33.0
87	21.0
88	13.0
89	11.0
90	8.5
91	5.5
92	3.0
93	1.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.816495145276994E-4
18-19	0.0
20-21	9.851334282880854E-4
22-23	5.310431028121807E-4
24-25	7.128607914264372E-4
26-27	0.002293172770223319
28-29	0.0021478112509069605
30-31	0.0023053650047974716
32-33	0.001159389250606624
34-35	0.0011638045773209897
36-37	0.00716455195339381
38-39	0.003246456767743263
40-41	7.719100907982691E-4
42-43	8.736418963707823E-5
44-45	5.298108369274621E-4
46-47	0.0029793642528279565
48-49	2.487495810830919E-4
50-51	0.0015015851632409817
52-53	0.0030269975834854405
54-55	0.0017438366274199625
56-57	1.8114849088342655E-4
58-59	0.0019531429058860152
60-61	3.4693929748342336E-4
62-63	0.0014120107923331242
64-65	0.0033115237972624017
66-67	0.004332401950551068
68-69	0.0019236751788869616
70-71	0.0018983305685852233
72-73	0.0013839007776216803
74-75	0.023768184733102504
76-77	0.008310242624235223
78-79	0.002373491111909925
80-81	0.005330679512746482
82-83	0.010901569089230323
84-85	0.0052318776698068335
86-87	0.0071064755549725255
88-89	0.009290831423036246
90-91	2.4824900049987415E-4
92-93	6.069873961090489E-5
94-95	3.6140716668346353E-4
96-97	0.003462014059600165
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	158.0
20-21	529.0
22-23	1101.0
24-25	2426.0
26-27	3923.0
28-29	8694.0
30-31	18456.0
32-33	24650.0
34-35	35721.0
36-37	53415.0
38-39	60975.0
40-41	62560.0
42-43	69288.0
44-45	81336.0
46-47	80905.0
48-49	75354.0
50-51	68919.0
52-53	67620.0
54-55	73012.0
56-57	76723.0
58-59	75577.0
60-61	72078.0
62-63	70280.0
64-65	75082.0
66-67	78038.0
68-69	79319.0
70-71	77529.0
72-73	73035.0
74-75	75774.0
76-77	75105.0
78-79	78962.0
80-81	79108.0
82-83	76787.0
84-85	77316.0
86-87	82291.0
88-89	86414.0
90-91	92286.0
92-93	96426.0
94-95	117428.0
96-97	264736.0
98-99	1505041.0
100-101	2982262.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.36286538097406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.12276370486997	78.69622974985995
2	6.234847690602875	10.769186235488263
3	1.4537773346560767	3.766571287404421
4	0.5114669223485244	1.7668699584642695
5	0.23654642300430725	1.0214413443135968
6	0.1280807216328938	0.6636850872167772
7	0.07679054329225585	0.4642295947016667
8	0.05288097385335162	0.3653561940889452
9	0.03677813469167155	0.28586385858061464
>10	0.1426308156612847	1.9642591507066456
>50	0.002900297736074478	0.1585437299937896
>100	5.096149399617076E-4	0.06530111363033002
>500	2.6822710798488585E-5	0.01246269555071356
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3973039579053798E-5
2	0.0	4.1919118737161396E-5	0.0	0.0	1.3973039579053798E-5
3	0.0	4.1919118737161396E-5	0.0	0.0	1.3973039579053798E-5
4	0.0	1.6767647494864558E-4	0.0	0.0	1.3973039579053798E-5
5	0.0	1.6767647494864558E-4	0.0	0.0	1.3973039579053798E-5
6	0.0	1.6767647494864558E-4	0.0	0.0	1.3973039579053798E-5
7	0.0	1.6767647494864558E-4	0.0	0.0	1.3973039579053798E-5
8	0.0	1.8164951452769937E-4	0.0	0.0	4.1919118737161396E-5
9	0.0	3.912451082135064E-4	0.0	0.0	4.1919118737161396E-5
10-11	0.0	3.9823162800303323E-4	0.0	0.0	4.1919118737161396E-5
12-13	0.0	4.1919118737161396E-4	0.0	0.0	4.1919118737161396E-5
14-15	0.0	5.868676623202596E-4	0.0	6.986519789526899E-6	6.28786781057421E-5
16-17	6.986519789526899E-6	6.427598206364747E-4	0.0	1.3973039579053798E-5	9.78112770533766E-5
18-19	1.3973039579053798E-5	6.846789393736361E-4	0.0	1.3973039579053798E-5	1.1178431663243038E-4
20-21	1.3973039579053798E-5	7.475576174793782E-4	0.0	2.7946079158107596E-5	1.1178431663243038E-4
22-23	1.3973039579053798E-5	8.244093351641741E-4	0.0	4.1919118737161396E-5	1.257573562114842E-4
24-25	1.3973039579053798E-5	9.431801715861314E-4	0.0	5.589215831621519E-5	1.4671691558006489E-4
26-27	1.3973039579053798E-5	9.781127705337659E-4	0.0	7.68517176847959E-5	1.537034353695918E-4
28-29	1.3973039579053798E-5	9.920858101128197E-4	0.0	4.2617770716114087E-4	1.537034353695918E-4
30-31	2.7946079158107596E-5	0.0010898970871661964	0.0	0.0019771851004361128	1.6767647494864558E-4
32-33	2.7946079158107596E-5	0.001110856646534777	0.0	0.004897550372458356	1.6767647494864558E-4
34-35	4.1919118737161396E-5	0.0012296274829567341	0.0	0.008600405860907614	1.956225541067532E-4
36-37	5.589215831621519E-5	0.0013483983193786917	0.0	0.014224554291476767	1.956225541067532E-4
38-39	5.589215831621519E-5	0.00139730395790538	0.0	0.02300660966691208	1.956225541067532E-4
40-41	6.986519789526899E-5	0.0014951152349587564	0.0	0.03317898248046325	2.0260907389628007E-4
42-43	6.986519789526899E-5	0.001523061314116864	0.0	0.042708595473377936	2.165821134753339E-4
44-45	6.986519789526899E-5	0.0017116973484340905	0.0	0.053216321236826396	2.3055515305438768E-4
46-47	6.986519789526899E-5	0.001872387303593209	0.0	0.06503052620091637	2.515147124229684E-4
48-49	6.986519789526899E-5	0.0018793738233827358	0.0	0.07653033777447765	2.7946079158107595E-4
50-51	6.986519789526899E-5	0.0022077402534905	0.0	0.08786247287309029	2.7946079158107595E-4
52-53	6.986519789526899E-5	0.0022356863326486076	0.0	0.09930639228833535	2.8644731137060286E-4
54-55	6.986519789526899E-5	0.0022496593722276612	0.0	0.1111625163711625	2.9343383116012977E-4
56-57	6.986519789526899E-5	0.002291578490964823	0.0	0.12134886222429273	2.9343383116012977E-4
58-59	6.986519789526899E-5	0.002305551530543877	0.0	0.13143739680036953	2.9343383116012977E-4
60-61	6.986519789526899E-5	0.0023334976097019846	0.0	0.14044302080906973	2.9343383116012977E-4
62-63	6.986519789526899E-5	0.00241034932738678	0.0	0.14917617054597837	2.9343383116012977E-4
64-65	6.986519789526899E-5	0.0026548775200202217	0.0	0.15786740116414982	2.9343383116012977E-4
66-67	6.986519789526899E-5	0.0027037831585469104	0.0	0.1661464271147392	2.9343383116012977E-4
68-69	6.986519789526899E-5	0.002773648356442179	0.0	0.1750053342078593	2.9343383116012977E-4
70-71	6.986519789526899E-5	0.002801594435600287	0.0	0.182068705715071	3.2137991031823735E-4
72-73	6.986519789526899E-5	0.0028085809553898135	0.0	0.18797231493722122	4.1919118737161396E-4
74-75	6.986519789526899E-5	0.002843513554337448	0.0	0.19226902460778028	4.1919118737161396E-4
76-77	6.986519789526899E-5	0.0028994057126536634	0.0	0.19647490952107546	4.1919118737161396E-4
78-79	6.986519789526899E-5	0.0029343383116012976	0.0	0.199080881402569	4.2617770716114087E-4
80-81	6.986519789526899E-5	0.0029413248313908246	0.0	0.19987035813878554	4.4713726652972154E-4
82-83	6.986519789526899E-5	0.0029483113511803516	0.0	0.20042229320215815	4.6111030610877535E-4
84-85	6.986519789526899E-5	0.0029622843907594052	0.0	0.2006109292364754	4.6111030610877535E-4
86-87	6.986519789526899E-5	0.002990230469917513	0.0	0.20070175399373924	4.7508334568782917E-4
88	6.986519789526899E-5	0.003046122628233728	0.0	0.20070874051352877	4.7508334568782917E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7900	0.0	46.04607	1
GTATCAA	13335	0.0	32.11819	1
TCAACGC	15520	0.0	26.881327	4
ATCAACG	15925	0.0	26.249311	3
CAACGCA	16190	0.0	25.768883	5
TATCAAC	16500	0.0	25.459116	2
AACGCAG	16695	0.0	25.11251	6
GTACATG	20935	0.0	23.167833	1
TACATGG	21260	0.0	22.562334	2
ACATGGG	21950	0.0	21.403662	3
ACGCAGA	19815	0.0	21.13764	7
CATGGGG	16955	0.0	19.854664	4
CGCAGAG	21250	0.0	19.768255	8
GTGGTAT	4175	0.0	19.099485	1
GCAGAGT	24160	0.0	17.370216	9
GAGTACT	13035	0.0	17.16984	12-13
CAGAGTA	23150	0.0	16.823164	10-11
TGGTATC	4405	0.0	16.609268	2
ATGGGGG	9825	0.0	16.60865	5
AGAGTAC	21270	0.0	16.310015	10-11
>>END_MODULE