FastQCFastQC Report
Fri 27 May 2016
SRR2138695_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138695_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9020220
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94400.044.044271
GTATCAA154950.032.220981
TCAACGC177650.027.4058674
ATCAACG184350.026.409833
CAACGCA186750.026.136825
AACGCAG194250.025.3617176
TATCAAC195000.025.3489512
GTACATG247950.021.552511
ACGCAGA233200.021.0902567
CGCAGAG237150.020.7389748
TACATGG252450.020.61172
ACATGGG258650.019.6542323
GTGGTAT53600.018.428781
TGGTATC49850.017.6594772
CATGGGG199850.017.4956344
GAGTACT154550.017.19512712-13
GCAGAGT287550.016.902759
GAGTACA190000.016.488421
CAGAGTA277900.016.2181210-11
TATACCG6950.016.0561855
Produced by FastQC (version 0.11.5)