##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138695_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9020220
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.115778329131665	33.0	31.0	34.0	30.0	34.0
2	32.38251561491848	34.0	31.0	34.0	31.0	34.0
3	32.40375312353801	34.0	31.0	34.0	30.0	34.0
4	36.04808230841376	37.0	35.0	37.0	35.0	37.0
5	35.78913186152888	37.0	35.0	37.0	35.0	37.0
6	35.88541620936075	37.0	35.0	37.0	35.0	37.0
7	35.843585965752496	37.0	35.0	37.0	35.0	37.0
8	35.8560389879626	37.0	35.0	37.0	35.0	37.0
9	37.63708401790644	39.0	37.0	39.0	35.0	39.0
10-11	37.54377720277333	39.0	37.0	39.0	35.0	39.0
12-13	37.502877590568744	39.0	37.0	39.0	35.0	39.0
14-15	38.78483396192111	40.0	38.0	41.0	35.0	41.0
16-17	38.73572706652388	40.0	38.0	41.0	34.0	41.0
18-19	38.731157056036324	40.0	38.0	41.0	34.0	41.0
20-21	38.74250927270076	40.0	38.0	41.0	34.0	41.0
22-23	38.66434263734118	40.0	38.0	41.0	34.0	41.0
24-25	38.58779350827227	40.0	38.0	41.0	34.0	41.0
26-27	38.423435588317545	40.0	38.0	41.0	34.0	41.0
28-29	38.29081625897054	40.0	38.0	41.0	34.0	41.0
30-31	38.15860544633709	40.0	38.0	41.0	33.5	41.0
32-33	38.004028346211	40.0	37.0	41.0	33.0	41.0
34-35	37.962800592543935	40.0	37.0	41.0	33.0	41.0
36-37	37.98323616795199	40.0	37.0	41.0	33.0	41.0
38-39	37.94791435821551	40.0	37.0	41.0	33.0	41.0
40-41	37.98290980653468	40.0	37.0	41.0	33.0	41.0
42-43	38.04599230438658	40.0	37.0	41.0	33.0	41.0
44-45	38.0262145804128	40.0	37.0	41.0	33.0	41.0
46-47	37.92745581171774	40.0	37.0	41.0	33.0	41.0
48-49	37.844758635207924	40.0	37.0	41.0	33.0	41.0
50-51	37.74747363263035	40.0	37.0	41.0	33.0	41.0
52-53	37.574468661793034	40.0	36.0	41.0	32.0	41.0
54-55	37.442083496100224	39.0	36.0	41.0	32.0	41.0
56-57	37.276128817438085	39.0	36.0	41.0	31.5	41.0
58-59	37.04450988702672	39.0	35.0	41.0	31.0	41.0
60-61	36.85099678647559	39.0	35.0	40.0	31.0	41.0
62-63	36.613966867320855	38.0	35.0	40.0	31.0	41.0
64-65	36.30248064807559	38.0	35.0	40.0	30.5	41.0
66-67	35.96505372841467	37.0	34.0	40.0	30.0	41.0
68-69	35.704114224208766	36.5	34.0	39.0	30.0	41.0
70-71	35.306091858520105	36.0	34.0	39.0	29.0	40.5
72-73	35.01505892449879	36.0	34.0	39.0	29.0	40.0
74-75	34.69386899514832	35.0	34.0	37.5	29.0	40.0
76-77	33.329189353119645	34.5	31.5	36.0	27.0	39.0
78-79	34.11066692744152	35.0	33.0	37.0	29.0	39.0
80-81	34.07395572294899	35.0	33.0	36.0	29.0	39.0
82-83	33.84289803390672	35.0	33.0	36.0	29.0	37.5
84-85	33.65022880322247	35.0	33.0	35.5	29.0	37.0
86-87	33.43081097687029	35.0	33.0	35.0	29.0	37.0
88-89	33.33169304715595	35.0	33.0	35.0	29.0	36.0
90-91	33.24013602455605	35.0	33.0	35.0	29.0	36.0
92-93	33.07218318020746	35.0	33.0	35.0	29.0	36.0
94-95	33.103586787683255	35.0	33.0	35.0	29.0	35.5
96-97	33.15993426474479	35.0	33.0	35.0	29.0	35.0
98-99	33.26230322405286	35.0	33.0	35.0	29.0	35.0
100	32.03065394898968	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	8.0
20	40.0
21	287.0
22	1305.0
23	4592.0
24	12262.0
25	26490.0
26	48658.0
27	76931.0
28	112234.0
29	150714.0
30	192821.0
31	239227.0
32	297110.0
33	374364.0
34	500200.0
35	695574.0
36	1083458.0
37	1846371.0
38	2189607.0
39	1124545.0
40	43419.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.62653239056254	19.705339781069643	13.255419490877163	24.41270833749066
2	15.794592593085314	20.782286906527776	39.50040021196822	23.922720288418688
3	19.41521381961859	24.512428743423108	29.604898771870307	26.46745866508799
4	13.262481402892611	16.814612060459723	37.17738591741665	32.745520619231016
5	13.929150286800102	37.25007815773895	34.972883144757	13.847888410703952
6	32.219679786080604	36.36474498404695	17.635645250337575	13.779929979534867
7	28.16109806634428	31.682786007436626	21.827860074366257	18.328255851852838
8	25.533700951861483	36.285766865996614	20.268020070463912	17.912512111677987
9	25.688929981752107	16.267951335998458	20.58675952471226	37.45635915753718
10-11	25.25646824578558	26.255357408134174	27.951857050049778	20.536317296030475
12-13	25.474107061690294	23.586902536745225	28.443574546962267	22.495415854602214
14-15	22.425650372163872	25.214008084060037	25.75272000017738	26.607621543598714
16-17	22.43077183688316	28.44590962360185	26.527894198460366	22.595424341054628
18-19	22.46630902572221	27.661509364516608	28.058284609466288	21.813897000294894
20-21	23.036746470248232	26.80321934270875	28.071502539720566	22.088531647322444
22-23	22.814460197943923	26.74204367459476	27.929367927930965	22.514128199530347
24-25	22.644104822380456	26.839575153026225	27.992431476169344	22.523888548423983
26-27	22.589157002717997	26.998838789565294	27.956586384143367	22.45541782357334
28-29	22.55466170466463	27.06548080857231	27.943417456786335	22.436440029976726
30-31	22.748455370565424	26.93569245225585	27.824325487169467	22.491526690009263
32-33	22.514216094896494	27.111956254362855	27.800288429097968	22.573539221642683
34-35	22.686763592854533	27.11934618753782	27.795340382100502	22.39854983750715
36-37	22.734015800992534	27.07318514540041	27.697681232712902	22.49511782089416
38-39	22.50708330130025	27.181079213491387	27.838689897881828	22.473147587326533
40-41	22.793573704057415	27.046645255125508	27.64758444326746	22.512196597549615
42-43	22.62246482003315	27.154810061655475	27.547331752506445	22.675393365804933
44-45	22.679633562203232	27.179894581078234	27.47579938085194	22.664672475866592
46-47	22.77179449043696	27.23452960692836	27.242769473202394	22.750906429432284
48-49	22.62331870192513	27.376898540233874	27.225299224290744	22.774483533550256
50-51	22.658512353582204	27.457993874399932	27.186109916720426	22.69738385529744
52-53	22.85268845705887	27.322695952139863	27.063177937426858	22.761437653374404
54-55	22.786702070333856	27.197580105666002	27.135641691058648	22.88007613294149
56-57	22.737668564244228	27.383288430444686	27.141331001672107	22.73771200363898
58-59	22.734978744262627	27.41956534799714	27.1071938666257	22.738262041114535
60-61	22.777226272848118	27.34699569665276	27.15814477241489	22.717633258084238
62-63	22.763155343004147	27.4365108119483	27.09348290646315	22.706850938584406
64-65	22.748134185998563	27.428584416617557	27.058801755334432	22.764479642049448
66-67	22.77233703845249	27.41402981107087	26.960851786951245	22.85278136352539
68-69	22.71146282323461	27.44859066508453	27.036768691915782	22.803177819765082
70-71	22.734754186333472	27.277299173289272	27.069916635538377	22.91803000483888
72-73	22.737880367431764	27.298996708526268	27.02424951256403	22.93887341147794
74-75	22.568264840182646	27.484447211844472	27.05933305659333	22.88795489137955
76-77	22.789837605735652	27.340233361174487	27.00567707253087	22.86425196055899
78-79	22.66915501454834	27.37685177505684	26.960078121360787	22.993915089034036
80-81	22.687000128858433	27.409622087531105	26.99978888537311	22.90358889823735
82-83	22.71359779106391	27.38543449158253	27.06618150382799	22.834786213525568
84-85	22.71476293308617	27.407746438752984	27.025516309604182	22.851974318556667
86-87	22.672788564506114	27.39862238506257	27.09387052007166	22.834718530359652
88-89	22.66337150825978	27.39488414895454	27.06651394557566	22.87523039721002
90-91	22.68064012266404	27.438176680948306	27.09009912292133	22.791084073466326
92-93	22.73009387210993	27.44806939582997	27.088995491703304	22.732841240356798
94-95	22.583060370294074	27.483289940591128	27.16555708228732	22.768092606827476
96-97	22.62507202992284	27.55296305619062	27.20845927735005	22.61350563653649
98-99	23.732322118056583	29.585364416915738	27.34932478933625	19.33298867569143
100	30.58029752570397	41.06362673962268	28.356075734673354	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1881.0
1	1626.5
2	2017.5
3	2833.5
4	3679.5
5	5718.5
6	7682.0
7	9235.0
8	10454.0
9	10626.5
10	9806.0
11	8748.5
12	8978.5
13	11288.0
14	17653.5
15	26411.5
16	33377.0
17	37519.5
18	38918.5
19	38368.0
20	36836.5
21	35191.0
22	34921.5
23	37724.5
24	44066.0
25	53774.5
26	66956.0
27	81759.5
28	97731.0
29	116494.5
30	134586.0
31	152098.5
32	170984.5
33	189507.5
34	207715.5
35	226178.5
36	246884.5
37	267023.5
38	284741.0
39	300333.0
40	315764.5
41	331787.0
42	344867.0
43	356836.5
44	368168.0
45	377890.0
46	386419.5
47	393713.5
48	397496.5
49	397510.5
50	395661.0
51	391162.5
52	382512.0
53	370460.5
54	356819.0
55	338343.0
56	315222.5
57	289884.5
58	261096.0
59	227651.5
60	193374.0
61	161479.0
62	130916.5
63	102838.0
64	78667.5
65	59705.0
66	45034.0
67	34377.5
68	26185.0
69	19209.5
70	14011.0
71	10008.0
72	6989.0
73	4650.5
74	3018.0
75	1988.5
76	1330.0
77	879.5
78	590.0
79	410.0
80	277.5
81	178.0
82	125.5
83	80.0
84	50.5
85	38.0
86	32.5
87	29.0
88	22.0
89	13.5
90	9.5
91	7.0
92	4.5
93	2.5
94	1.5
95	1.0
96	1.0
97	1.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.2194824516475208E-4
18-19	0.0
20-21	8.924738621568022E-4
22-23	5.654685017413104E-4
24-25	7.319387460425403E-4
26-27	0.002274329767498596
28-29	0.0018150899585226965
30-31	0.002479172450012713
32-33	0.0011702875363039913
34-35	0.0011800439468499257
36-37	0.006707608756032631
38-39	0.0030932097983142233
40-41	7.997985878641875E-4
42-43	5.1856810899471946E-5
44-45	5.702754180046075E-4
46-47	0.0031604773886381955
48-49	2.3212122012912365E-4
50-51	0.0014135985412625505
52-53	0.0028978276984456103
54-55	0.001417714123163761
56-57	1.2411240239723105E-4
58-59	0.0017828674598125039
60-61	3.238740312911848E-4
62-63	0.001322827769108761
64-65	0.0032469061043458214
66-67	0.0040490029264563035
68-69	0.0015912548362662024
70-71	0.0016322693994543632
72-73	0.001147698713784982
74-75	0.022563055162173863
76-77	0.0081064434174103
78-79	0.0021728309502016966
80-81	0.005077729596566101
82-83	0.010570995963150693
84-85	0.00490413490430603
86-87	0.006967719764474536
88-89	0.009378071213315456
90-91	1.9426534906064155E-4
92-93	3.166994094268588E-5
94-95	3.2338294782313155E-4
96-97	0.0032285005370710496
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	249.0
20-21	627.0
22-23	1374.0
24-25	3356.0
26-27	4816.0
28-29	10013.0
30-31	21108.0
32-33	29101.0
34-35	41942.0
36-37	63429.0
38-39	73202.0
40-41	74255.0
42-43	81072.0
44-45	95783.0
46-47	96034.0
48-49	90735.0
50-51	82860.0
52-53	81520.0
54-55	88553.0
56-57	92386.0
58-59	91797.0
60-61	88453.0
62-63	85685.0
64-65	91141.0
66-67	96742.0
68-69	96762.0
70-71	95590.0
72-73	90275.0
74-75	92371.0
76-77	92895.0
78-79	99232.0
80-81	98668.0
82-83	95036.0
84-85	95858.0
86-87	102968.0
88-89	110466.0
90-91	118374.0
92-93	125724.0
94-95	153147.0
96-97	342712.0
98-99	1908642.0
100-101	3815267.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.92083483258837
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.44073550530484	77.70743497489107
2	6.6929448187779945	11.501268126357042
3	1.613767280062419	4.159686957854354
4	0.5905957983223175	2.0297793616189006
5	0.2610829218596637	1.1216231303356872
6	0.13912680083873802	0.7172334525390974
7	0.0767445084203275	0.46157665626038025
8	0.04481905907658228	0.3080712777816837
9	0.03149970462157339	0.24358328262589754
>10	0.10448784670679917	1.4487202411193758
>50	0.0034350336573207227	0.1969499631909533
>100	7.607223515202177E-4	0.10407257542554946
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1086204105886552E-5	1.1086204105886552E-5
2	0.0	2.2172408211773104E-5	0.0	3.3258612317659656E-5	1.1086204105886552E-5
3	0.0	2.2172408211773104E-5	0.0	3.3258612317659656E-5	1.1086204105886552E-5
4	0.0	5.5431020529432766E-5	0.0	6.651722463531931E-5	1.1086204105886552E-5
5	0.0	5.5431020529432766E-5	0.0	6.651722463531931E-5	1.1086204105886552E-5
6	6.651722463531931E-5	5.5431020529432766E-5	0.0	7.760342874120586E-5	1.1086204105886552E-5
7	6.651722463531931E-5	6.651722463531931E-5	0.0	7.760342874120586E-5	1.1086204105886552E-5
8	6.651722463531931E-5	8.868963284709242E-5	0.0	7.760342874120586E-5	1.1086204105886552E-5
9	7.760342874120586E-5	2.7715510264716384E-4	0.0	1.2194824516475208E-4	3.3258612317659656E-5
10-11	7.760342874120586E-5	2.7715510264716384E-4	0.0	1.9400857185301467E-4	3.3258612317659656E-5
12-13	7.760342874120586E-5	2.826982047001071E-4	0.0	2.2172408211773107E-4	3.3258612317659656E-5
14-15	7.760342874120586E-5	3.824740416530861E-4	0.0	2.3281028622361763E-4	4.988791847648949E-5
16-17	2.161809800647878E-4	3.9910334781191587E-4	0.0	2.60525796488334E-4	5.5431020529432766E-5
18-19	2.2172408211773107E-4	4.3236196012957554E-4	0.0	2.7715510264716384E-4	5.5431020529432766E-5
20-21	2.2172408211773107E-4	4.656205724472352E-4	0.0	3.325861231765966E-4	5.5431020529432766E-5
22-23	2.2172408211773107E-4	4.933360827119516E-4	0.0	3.713878375471995E-4	7.20603266882626E-5
24-25	2.328102862236176E-4	5.487671032413843E-4	0.0	4.101895519178024E-4	8.868963284709242E-5
26-27	2.328102862236176E-4	5.764826135061008E-4	0.0	4.656205724472352E-4	8.868963284709242E-5
28-29	2.328102862236176E-4	5.82025715559044E-4	0.0	8.203791038356049E-4	9.423273490003568E-5
30-31	2.328102862236176E-4	6.984308586708528E-4	0.0	0.002028775351377239	1.1086204105886552E-4
32-33	2.549826944353907E-4	7.095170627767393E-4	0.0	0.004473283356725224	1.3303444927063862E-4
34-35	2.7161200059422054E-4	7.982066956238318E-4	0.0	0.008181618630144275	1.4966375542946846E-4
36-37	2.7715510264716384E-4	8.924394305238675E-4	0.0	0.013769065499511098	1.5520685748241173E-4
38-39	2.7715510264716384E-4	9.42327349000357E-4	0.0	0.02255488225342619	1.5520685748241173E-4
40-41	2.7715510264716384E-4	0.0010088445736356763	0.0	0.031673285130517884	1.5520685748241173E-4
42-43	2.882413067530504E-4	0.0010587324921121659	0.0	0.04012097265920343	1.5520685748241173E-4
44-45	2.882413067530504E-4	0.0011806807372769178	0.0	0.04982694435390711	1.5520685748241173E-4
46-47	2.882413067530504E-4	0.0012749134721769535	0.0	0.06117367425628199	1.5520685748241173E-4
48-49	3.0487061291188016E-4	0.0012749134721769535	0.0	0.07172774056508599	1.7183616364124156E-4
50-51	3.5475853138836966E-4	0.001463378941977025	0.0	0.08213214311846052	1.7737926569418483E-4
52-53	3.6584473549425625E-4	0.001463378941977025	0.0	0.09225939056918789	1.8292236774712813E-4
54-55	3.6584473549425625E-4	0.001463378941977025	0.0	0.10254738797945062	1.884654698000714E-4
56-57	3.6584473549425625E-4	0.001485551350188798	0.0	0.11245291134806024	1.9400857185301467E-4
58-59	3.6584473549425625E-4	0.001485551350188798	0.0	0.12174869349084613	1.9955167390595793E-4
60-61	3.713878375471995E-4	0.0015077237584005712	0.0	0.13044027750986117	1.9955167390595793E-4
62-63	3.769309396001428E-4	0.0015520685748241173	0.0	0.1387438443851702	1.9955167390595793E-4
64-65	3.824740416530861E-4	0.0017072754323065292	0.0	0.1472358767302793	1.9955167390595793E-4
66-67	3.8801714370602933E-4	0.0017072754323065292	0.0	0.15468026278738212	2.050947759589012E-4
68-69	3.8801714370602933E-4	0.0017349909425712455	0.0	0.16270113145799103	2.106378780118445E-4
70-71	4.21275756023689E-4	0.0017460771466771322	0.0	0.16943600045231713	2.2172408211773107E-4
72-73	4.7670677655312175E-4	0.0017516202487300753	0.0	0.1744913095246014	2.6606889854127725E-4
74-75	4.7670677655312175E-4	0.001784878861047735	0.0	0.1784102826760323	2.826982047001071E-4
76-77	4.7670677655312175E-4	0.0018680253918418842	0.0	0.18212970415355723	2.882413067530504E-4
78-79	4.7670677655312175E-4	0.0019179133103183737	0.0	0.18455203975069345	2.882413067530504E-4
80-81	4.7670677655312175E-4	0.0019289995144242602	0.0	0.18534470334426434	2.993275108589369E-4
82-83	4.7670677655312175E-4	0.0019400857185301467	0.0	0.185865754937241	3.436723272824831E-4
84-85	4.8224987860606504E-4	0.0019511719226360334	0.0	0.18599878938651165	3.5475853138836966E-4
86-87	4.988791847648948E-4	0.0019622581267419196	0.0	0.18611519452962344	3.5475853138836966E-4
88	5.099653888707814E-4	0.002028775351377239	0.0	0.18611519452962344	3.5475853138836966E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9440	0.0	44.04427	1
GTATCAA	15495	0.0	32.22098	1
TCAACGC	17765	0.0	27.405867	4
ATCAACG	18435	0.0	26.40983	3
CAACGCA	18675	0.0	26.13682	5
AACGCAG	19425	0.0	25.361717	6
TATCAAC	19500	0.0	25.348951	2
GTACATG	24795	0.0	21.55251	1
ACGCAGA	23320	0.0	21.090256	7
CGCAGAG	23715	0.0	20.738974	8
TACATGG	25245	0.0	20.6117	2
ACATGGG	25865	0.0	19.654232	3
GTGGTAT	5360	0.0	18.42878	1
TGGTATC	4985	0.0	17.659477	2
CATGGGG	19985	0.0	17.495634	4
GAGTACT	15455	0.0	17.195127	12-13
GCAGAGT	28755	0.0	16.90275	9
GAGTACA	19000	0.0	16.48842	1
CAGAGTA	27790	0.0	16.21812	10-11
TATACCG	695	0.0	16.056185	5
>>END_MODULE