FastQCFastQC Report
Fri 27 May 2016
SRR2138694_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138694_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15002537
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC237400.050.837031
GGTATCA167700.043.9180031
TATACAC302900.041.5162053
TTATACA306950.039.8551522
GTATCAA286150.032.580461
TACACAT412950.030.4133135
ACACATC450550.027.1881486
ATACACA480100.026.9550084
TCAACGC337550.026.8570694
ATCAACG344400.026.3462373
ACATCTC470000.026.174228
CAACGCA349250.025.9228155
AACGCAG354950.025.6990766
TATCAAC358400.025.5077782
CACATCT489350.024.9174167
CATCTCC546300.022.3272159
GTACATG462800.022.2901761
TACATGG457550.022.1329752
ACATGGG464100.021.3441723
ACGCAGA428150.021.3147417
Produced by FastQC (version 0.11.5)