FastQCFastQC Report
Fri 27 May 2016
SRR2138693_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138693_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8979629
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104200.045.364611
CTTATAC101150.042.6373831
TATACAC135850.034.377173
GTATCAA178100.032.1732521
TTATACA139850.031.6225812
TCAACGC209600.026.7374324
ATCAACG213350.026.3436133
CAACGCA215900.025.9948485
TACACAT180150.025.6531055
TATCAAC221800.025.5597042
AACGCAG221550.025.5518866
ACACATC183800.024.0389396
ACATCTC192650.023.124358
CACATCT202700.021.8776577
ATACACA223350.021.8004344
ACGCAGA264000.021.3663447
GTACATG271550.021.3106631
TACATGG272450.020.8229142
CGCAGAG272200.020.7226858
ACATGGG277200.020.0705473
Produced by FastQC (version 0.11.5)