FastQCFastQC Report
Fri 27 May 2016
SRR2138692_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138692_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12004269
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA138050.045.322311
GTATCAA238700.034.259671
TCAACGC288500.027.6186874
ATCAACG291350.027.3767593
TATCAAC303600.026.5160162
CAACGCA300750.026.5074125
AACGCAG307950.026.074676
GTACATG359250.023.2329581
TACATGG367450.022.1995262
ACGCAGA361850.022.1338317
CGCAGAG374100.021.4420418
ACATGGG373650.021.401813
GTGGTAT76550.019.2378731
CATGGGG287800.018.9098594
GCAGAGT435400.018.3665289
GAGTACT247600.017.90973512-13
TGGTATC76950.017.8548872
AGAGTAC386100.017.13196810-11
CAGAGTA417350.016.9728110-11
GAGTACA257500.016.2785681
Produced by FastQC (version 0.11.5)