##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138692_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12004269
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.163108140945525	33.0	31.0	34.0	30.0	34.0
2	32.42111993658256	34.0	31.0	34.0	31.0	34.0
3	32.44074262247872	34.0	31.0	34.0	30.0	34.0
4	36.07510278218524	37.0	35.0	37.0	35.0	37.0
5	35.83084034521386	37.0	35.0	37.0	35.0	37.0
6	35.92276356019679	37.0	35.0	37.0	35.0	37.0
7	35.88231353362708	37.0	35.0	37.0	35.0	37.0
8	35.895109398164934	37.0	35.0	37.0	35.0	37.0
9	37.681520882279465	39.0	37.0	39.0	35.0	39.0
10-11	37.591334132882224	39.0	37.0	39.0	35.0	39.0
12-13	37.55063919344026	39.0	37.0	39.0	35.0	39.0
14-15	38.840413314629984	40.0	38.0	41.0	35.0	41.0
16-17	38.79878137519244	40.0	38.0	41.0	35.0	41.0
18-19	38.80170042007556	40.0	38.0	41.0	35.0	41.0
20-21	38.816067321236744	40.0	38.0	41.0	34.5	41.0
22-23	38.740906786292086	40.0	38.0	41.0	34.0	41.0
24-25	38.66722574447752	40.0	38.0	41.0	34.0	41.0
26-27	38.50798051217146	40.0	38.0	41.0	34.0	41.0
28-29	38.37905622624609	40.0	38.0	41.0	34.0	41.0
30-31	38.24529546038532	40.0	38.0	41.0	34.0	41.0
32-33	38.089235331855946	40.0	37.5	41.0	33.0	41.0
34-35	38.05004339622861	40.0	37.5	41.0	33.0	41.0
36-37	38.06760461379547	40.0	37.5	41.0	33.0	41.0
38-39	38.03283629804268	40.0	37.0	41.0	33.0	41.0
40-41	38.064900481342654	40.0	37.0	41.0	33.0	41.0
42-43	38.11933739183979	40.0	38.0	41.0	33.0	41.0
44-45	38.10177399914268	40.0	37.0	41.0	33.0	41.0
46-47	38.007225676751425	40.0	37.0	41.0	33.0	41.0
48-49	37.92716774751226	40.0	37.0	41.0	33.0	41.0
50-51	37.82682629925981	40.0	37.0	41.0	33.0	41.0
52-53	37.65945387392978	40.0	36.5	41.0	32.5	41.0
54-55	37.52713309646296	39.0	36.0	41.0	32.0	41.0
56-57	37.3670096072957	39.0	36.0	41.0	32.0	41.0
58-59	37.14397316202843	39.0	35.0	41.0	31.0	41.0
60-61	36.95074373752158	39.0	35.0	40.5	31.0	41.0
62-63	36.7160189229819	38.0	35.0	40.0	31.0	41.0
64-65	36.40901908114677	38.0	35.0	40.0	31.0	41.0
66-67	36.08116771046721	37.0	35.0	40.0	30.0	41.0
68-69	35.820300958194025	37.0	34.0	39.5	30.0	41.0
70-71	35.43484287714388	36.0	34.0	39.0	30.0	41.0
72-73	35.142934589341486	36.0	34.0	39.0	30.0	40.0
74-75	34.82360459490231	35.0	34.0	38.0	29.0	40.0
76-77	33.452311170009516	34.5	32.0	36.0	28.0	39.0
78-79	34.22196589140418	35.0	33.5	37.0	29.0	39.0
80-81	34.181707608582414	35.0	34.0	36.0	29.0	39.0
82-83	33.952556688985645	35.0	34.0	36.0	29.0	37.5
84-85	33.76179439567584	35.0	33.0	36.0	29.0	37.0
86-87	33.539639346260486	35.0	33.0	35.0	29.0	37.0
88-89	33.437798322947174	35.0	33.0	35.0	29.0	36.0
90-91	33.34418891190329	35.0	33.0	35.0	29.0	36.0
92-93	33.182793400755486	35.0	33.0	35.0	29.0	36.0
94-95	33.204158425511494	35.0	33.0	35.0	29.0	35.5
96-97	33.25524520473313	35.0	33.0	35.0	29.0	35.0
98-99	33.34961293007143	35.0	33.0	35.0	29.5	35.0
100	32.16489742045627	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	12.0
20	80.0
21	355.0
22	1752.0
23	5868.0
24	15448.0
25	33234.0
26	60847.0
27	97927.0
28	141526.0
29	190253.0
30	243010.0
31	304279.0
32	379309.0
33	481978.0
34	648305.0
35	904251.0
36	1420452.0
37	2456193.0
38	2971348.0
39	1583768.0
40	64074.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.76910988915693	19.5729869099068	13.392543935828163	24.265359265108106
2	15.658404522591088	20.683691776650456	39.89324964310613	23.764654057652322
3	19.182109297950586	24.79129716270104	29.767585181571654	26.259008357776718
4	13.20357782718798	16.854270759843853	37.387832611881656	32.55431880108651
5	13.785754051329574	37.4383729654842	34.97466609586973	13.801206887316503
6	31.918228423571648	36.45768851064567	17.788421768955693	13.835661296826988
7	27.997306624834884	31.957339509802722	21.948791717346555	18.096562148015842
8	25.53947266593243	36.20202946135246	20.31088273679972	17.9476151359154
9	25.860191903397034	16.32719993195754	20.659300453863537	37.15330771078189
10-11	25.215213021301004	26.209850845561693	27.98930113945297	20.58563499368433
12-13	25.550643691839962	23.63205539629277	28.455435312221013	22.361865599646258
14-15	22.52147965028108	25.156483914180868	25.975646663699386	26.34638977183867
16-17	22.380629499368705	28.337305605883785	26.68860250243799	22.593462392309526
18-19	22.402834358343686	27.541223043235703	28.321824510930238	21.734118087490376
20-21	23.049478862722683	26.68133637007607	28.31442283685189	21.95476193034936
22-23	22.8433321732981	26.569582243179322	28.159983050210585	22.427102533311995
24-25	22.62088681687581	26.68467142974111	28.246260657384276	22.448181095998805
26-27	22.539888062470244	26.88101906006158	28.17246099986744	22.406631877600738
28-29	22.508790144524752	26.993793518401628	28.14465391749784	22.35276241957578
30-31	22.745650469579047	26.81444245270594	28.023373434724324	22.41653364299069
32-33	22.465402277601015	26.993085382343047	28.041169454151216	22.50034288590472
34-35	22.61358640820197	27.007773984312234	28.05548911844315	22.323150489042646
36-37	22.68459656085441	26.99081286507382	27.9261629011691	22.398427672902674
38-39	22.469688393358105	27.120192403459082	28.026007671824615	22.38411153135819
40-41	22.753171954584626	26.966290777179523	27.85171893133419	22.42881833690166
42-43	22.612236069872047	27.084250328506123	27.69277202873303	22.610741572888795
44-45	22.68706675225071	27.157295658733048	27.575185261394985	22.580452327621256
46-47	22.698355272386838	27.188486322393977	27.358784826947414	22.754373578271768
48-49	22.65876588866485	27.35489690157351	27.272039854808305	22.71429735495333
50-51	22.67500616131681	27.424385156729336	27.209561870690358	22.691046811263494
52-53	22.802839882632156	27.29560162089242	27.112509556800607	22.78904893967482
54-55	22.7597094412756	27.205013328351924	27.19390209606126	22.841375134311214
56-57	22.72091730239912	27.36139341970484	27.139253923510747	22.778435354385294
58-59	22.736544073339225	27.445373933403435	27.125731733537627	22.692350259719717
60-61	22.80859357854673	27.36070427908776	27.157132046058663	22.673570096306847
62-63	22.789720397997286	27.40712716380289	27.111686358250125	22.691466079949695
64-65	22.728658991343504	27.374725377949716	27.13300126798896	22.76361436271782
66-67	22.802973707974804	27.37306643886928	26.983131912320896	22.84082794083502
68-69	22.71271523944371	27.384549485114874	27.10901914959578	22.793716125845627
70-71	22.732352662605507	27.198121231934536	27.139691160306402	22.92983494515356
72-73	22.827686692716462	27.221552341567467	27.06218147390725	22.88857949180882
74-75	22.624923818944513	27.370387309206446	27.093539322012205	22.911149549836836
76-77	22.79114078952979	27.213571985797845	27.032484589490185	22.96280263518218
78-79	22.705168912769675	27.231560605193483	27.051135114294578	23.01213536774226
80-81	22.729673318364377	27.261869839881612	27.079527027542806	22.9289298142112
82-83	22.775058744496903	27.259995022486706	27.12608200381285	22.838864229203544
84-85	22.85991817308237	27.233119423340028	27.0802241194485	22.826738284129107
86-87	22.753279316931398	27.218231075552303	27.142736173049503	22.8857534344668
88-89	22.752635689961444	27.137519004250844	27.17904106661255	22.93080423917516
90-91	22.79976410746036	27.175568369949566	27.16290737144229	22.861760151147788
92-93	22.840843883752946	27.08996087114229	27.2116166011451	22.85757864395967
94-95	22.682660604556464	27.14020194973758	27.299911641654163	22.8772258040518
96-97	22.72763871073817	27.241567696810343	27.340161079092194	22.690632513359294
98-99	23.858605387218162	29.220859558582763	27.505231303194538	19.41530375100454
100	22.86051929980472	30.201168746126594	21.15854371543166	25.77976823863703
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1900.0
1	1725.5
2	2615.0
3	3962.0
4	5420.5
5	8702.0
6	11793.0
7	14470.0
8	16741.0
9	17026.0
10	15267.0
11	13232.0
12	13611.5
13	17403.0
14	27417.5
15	41179.0
16	52380.5
17	59077.0
18	61206.0
19	59570.5
20	55938.0
21	52376.5
22	51020.0
23	54315.5
24	62598.5
25	75822.0
26	93757.0
27	113827.5
28	136021.5
29	161418.0
30	186040.0
31	209525.5
32	233529.0
33	256472.5
34	278838.0
35	300963.5
36	326058.0
37	351702.5
38	372578.0
39	389733.0
40	409636.5
41	428901.0
42	442144.0
43	456210.0
44	471602.0
45	487312.5
46	499472.0
47	508241.0
48	516266.5
49	521509.0
50	523148.0
51	521161.5
52	512043.5
53	496256.5
54	479011.0
55	457602.0
56	430930.5
57	398348.5
58	360163.5
59	317797.5
60	273142.5
61	228596.5
62	185957.0
63	148112.5
64	115442.5
65	88880.0
66	68260.0
67	52847.0
68	40928.0
69	30788.5
70	22678.5
71	16488.0
72	11857.0
73	8278.5
74	5626.5
75	3822.0
76	2560.0
77	1681.5
78	1143.5
79	776.0
80	516.0
81	338.5
82	244.5
83	174.5
84	123.0
85	88.5
86	69.0
87	50.5
88	31.5
89	19.0
90	12.5
91	9.0
92	6.5
93	3.0
94	1.5
95	2.0
96	2.0
97	2.0
98	1.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.2912073196626967E-4
18-19	0.0
20-21	8.705608840499959E-4
22-23	5.665450011276746E-4
24-25	8.000201005050252E-4
26-27	0.0024344527758681034
28-29	0.0020066801171250374
30-31	0.0027083974992964644
32-33	0.001379491366019312
34-35	0.0010611110708749771
36-37	0.006694849440463127
38-39	0.0029707368739701
40-41	7.853954761824722E-4
42-43	9.585685747758431E-5
44-45	6.211280266445422E-4
46-47	0.0032961353237054665
48-49	1.9863260412445255E-4
50-51	0.0014926619914849796
52-53	0.003117855107554009
54-55	0.0017803754895799563
56-57	1.2726259339954917E-4
58-59	0.0018501167933909252
60-61	3.5692298431603235E-4
62-63	0.0013703139286802804
64-65	0.00352056093876497
66-67	0.004076673801427963
68-69	0.002000730367631174
70-71	0.0016936707371049487
72-73	0.0012381486124394875
74-75	0.02318198762183587
76-77	0.007911526409020288
78-79	0.0023450891144620786
80-81	0.005599413386479963
82-83	0.01081182070286784
84-85	0.004706145738912783
86-87	0.007188662030879423
88-89	0.010313076611789018
90-91	3.228338756746788E-4
92-93	1.1953398718822772E-5
94-95	3.536760478628576E-4
96-97	0.0038470929667559165
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	340.0
20-21	972.0
22-23	2015.0
24-25	4927.0
26-27	7575.0
28-29	17291.0
30-31	35935.0
32-33	46283.0
34-35	65784.0
36-37	97565.0
38-39	110760.0
40-41	111369.0
42-43	119290.0
44-45	139211.0
46-47	136943.0
48-49	125629.0
50-51	114463.0
52-53	110820.0
54-55	118204.0
56-57	122809.0
58-59	120647.0
60-61	114652.0
62-63	111487.0
64-65	118045.0
66-67	123550.0
68-69	125054.0
70-71	121963.0
72-73	116165.0
74-75	118993.0
76-77	118174.0
78-79	125196.0
80-81	125838.0
82-83	121320.0
84-85	123994.0
86-87	132316.0
88-89	142435.0
90-91	151618.0
92-93	161506.0
94-95	190431.0
96-97	438616.0
98-99	772564.0
100-101	6841520.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.6944044182545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.29541348922385	74.73526449262359
2	7.1945479433662305	12.04286810357229
3	1.9447362264383556	4.882906206670857
4	0.6986368723162406	2.338879877325595
5	0.322268294660164	1.3486026492234486
6	0.17787065264800608	0.8932067002116656
7	0.10879072777603875	0.6373622617210816
8	0.06342115338861526	0.4246396528303134
9	0.04686713068619445	0.35302649306161893
>10	0.1416935823941572	1.9024917318796055
>50	0.004469970255102986	0.25050345328605833
>100	0.0012296851726284399	0.15899780361986693
>500	5.4271674447866E-5	0.03125057397395841
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.66429584342037E-5	0.0	0.0	0.0
3	0.0	6.66429584342037E-5	0.0	0.0	0.0
4	0.0	1.332859168684074E-4	0.0	0.0	8.330369804275462E-6
5	0.0	1.332859168684074E-4	0.0	0.0	8.330369804275462E-6
6	0.0	1.332859168684074E-4	0.0	0.0	8.330369804275462E-6
7	0.0	1.4161628667268286E-4	0.0	0.0	8.330369804275462E-6
8	0.0	1.749377658897847E-4	0.0	0.0	8.330369804275462E-6
9	0.0	3.41545161975294E-4	0.0	2.4991109412826386E-5	8.330369804275462E-6
10-11	0.0	3.41545161975294E-4	0.0	6.66429584342037E-5	1.6660739608550924E-5
12-13	0.0	3.41545161975294E-4	0.0	8.330369804275462E-5	1.6660739608550924E-5
14-15	0.0	4.4567478452873724E-4	0.0	9.163406784703009E-5	3.748666411923958E-5
16-17	0.0	4.540051543330127E-4	0.0	1.0829480745558101E-4	4.998221882565277E-5
18-19	0.0	4.7899626374583906E-4	0.0	1.4578147157482058E-4	5.4147403727790506E-5
20-21	0.0	5.039873731586654E-4	0.0	1.9159850549833565E-4	5.8312588629928234E-5
22-23	0.0	5.664651466907314E-4	0.0	2.2491998471543747E-4	6.247777353206596E-5
24-25	0.0	6.456036598313483E-4	0.0	3.0822368275819215E-4	6.66429584342037E-5
26-27	0.0	6.747599541463125E-4	0.0	4.3317922982232405E-4	6.66429584342037E-5
28-29	0.0	6.789251390484502E-4	0.0	0.0011579214027942894	7.913851314061689E-5
30-31	0.0	8.038806861125821E-4	0.0	0.0029781072050284775	9.163406784703009E-5
32-33	0.0	8.20541425721133E-4	0.0	0.006614313624594717	1.0829480745558101E-4
34-35	0.0	0.0010079747463173309	0.0	0.01101274888125216	1.0829480745558101E-4
36-37	0.0	0.0011537562178921515	0.0	0.017793669901932388	1.1245999235771873E-4
38-39	0.0	0.0012037384367178045	0.0	0.028027529206484793	1.207903621619942E-4
40-41	0.0	0.001366180647901176	0.0	0.03842383072222057	1.2495554706413192E-4
42-43	0.0	0.0014036673120204155	0.0	0.0483619618987212	1.332859168684074E-4
44-45	0.0	0.001549448783595236	0.0	0.0591039737613344	1.4161628667268286E-4
46-47	0.0	0.0016660739608550924	0.0	0.07083313444575426	1.4161628667268286E-4
48-49	8.330369804275462E-6	0.0016827347004636432	0.0	0.08201249072309193	1.4161628667268286E-4
50-51	8.330369804275462E-6	0.001874333205961979	0.0	0.0925962255594239	1.4161628667268286E-4
52-53	8.330369804275462E-6	0.0018826635757662545	0.0	0.1036131396255782	1.4161628667268286E-4
54-55	8.330369804275462E-6	0.0018951591304726678	0.0	0.11382200782071777	1.4161628667268286E-4
56-57	8.330369804275462E-6	0.0019159850549833562	0.0	0.12206490874204834	1.4578147157482058E-4
58-59	8.330369804275462E-6	0.0019326457945919072	0.0	0.13047858224436656	1.4994665647695833E-4
60-61	8.330369804275462E-6	0.0019701324587111466	0.0	0.13890891648629333	1.4994665647695833E-4
62-63	8.330369804275462E-6	0.001999288753026111	0.0	0.1467644552117251	1.6660739608550924E-4
64-65	8.330369804275462E-6	0.0022575302169586505	0.0	0.15382027843594642	1.6660739608550924E-4
66-67	1.6660739608550924E-5	0.0022825213263714767	0.0	0.16103437868644896	1.6660739608550924E-4
68-69	1.6660739608550924E-5	0.0023449990999035424	0.0	0.16807770635596386	1.6660739608550924E-4
70-71	1.6660739608550924E-5	0.0023824857640227823	0.0	0.1743088229695619	1.6660739608550924E-4
72-73	1.6660739608550924E-5	0.0023908161338270575	0.0	0.17880305747896852	1.8326813569406018E-4
74-75	1.6660739608550924E-5	0.002449128722456986	0.0	0.18239761204951338	1.8326813569406018E-4
76-77	1.6660739608550924E-5	0.0025324324204997403	0.0	0.18604631402378602	1.8326813569406018E-4
78-79	1.6660739608550924E-5	0.0025490931601082913	0.0	0.18849960793114517	1.8326813569406018E-4
80-81	1.6660739608550924E-5	0.002553258345010429	0.0	0.1892326804739214	1.9159850549833565E-4
82-83	2.0825924510688655E-5	0.0025657538997168423	0.0	0.18967835525845012	2.0825924510688656E-4
84-85	2.4991109412826386E-5	0.00260740574873822	0.0	0.18978248488100358	2.0825924510688656E-4
86-87	2.4991109412826386E-5	0.0026740487071724235	0.0	0.18985329302433993	2.0825924510688656E-4
88	2.4991109412826386E-5	0.002774013144823729	0.0	0.18986578857904635	2.0825924510688656E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13805	0.0	45.32231	1
GTATCAA	23870	0.0	34.25967	1
TCAACGC	28850	0.0	27.618687	4
ATCAACG	29135	0.0	27.376759	3
TATCAAC	30360	0.0	26.516016	2
CAACGCA	30075	0.0	26.507412	5
AACGCAG	30795	0.0	26.07467	6
GTACATG	35925	0.0	23.232958	1
TACATGG	36745	0.0	22.199526	2
ACGCAGA	36185	0.0	22.133831	7
CGCAGAG	37410	0.0	21.442041	8
ACATGGG	37365	0.0	21.40181	3
GTGGTAT	7655	0.0	19.237873	1
CATGGGG	28780	0.0	18.909859	4
GCAGAGT	43540	0.0	18.366528	9
GAGTACT	24760	0.0	17.909735	12-13
TGGTATC	7695	0.0	17.854887	2
AGAGTAC	38610	0.0	17.131968	10-11
CAGAGTA	41735	0.0	16.97281	10-11
GAGTACA	25750	0.0	16.278568	1
>>END_MODULE