FastQCFastQC Report
Fri 27 May 2016
SRR2138691_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138691_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4454809
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51400.046.4262851
GTATCAA88200.035.1488571
TCAACGC106050.028.2152444
ATCAACG107750.027.7315673
CAACGCA109700.027.2386175
TATCAAC112400.026.9166132
AACGCAG112650.026.6358346
GTACATG131300.022.283511
ACGCAGA135150.022.2014547
TACATGG132700.021.6731242
CGCAGAG138950.021.5644238
ACATGGG137650.020.5017953
CATGGGG102350.018.287234
GCAGAGT163300.018.2218389
GAGTACT92050.018.19193612-13
GTGGTAT27400.018.1756461
CAGAGTA155450.017.20645310-11
AGAGTAC143650.017.04533810-11
TGGTATC27050.016.8765872
GTACTTT106000.015.8967614-15
Produced by FastQC (version 0.11.5)