##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138691_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4454809
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62879037013708	34.0	31.0	34.0	31.0	34.0
2	32.81416285187535	34.0	31.0	34.0	31.0	34.0
3	32.84593772707203	34.0	31.0	34.0	31.0	34.0
4	35.24011354920042	35.0	34.0	37.0	34.0	37.0
5	35.083567219155746	35.0	34.0	37.0	34.0	37.0
6	36.39719996974057	37.0	37.0	38.0	35.0	38.0
7	36.36160181053778	37.0	37.0	38.0	35.0	38.0
8	36.32228160623721	37.0	37.0	38.0	35.0	38.0
9	37.48504144622137	38.0	38.0	39.0	35.0	39.0
10-11	37.43499609074149	38.0	37.0	39.0	35.0	39.0
12-13	37.37317817666256	38.0	37.0	39.0	35.0	39.0
14-15	38.23846027966631	38.0	38.0	41.0	35.0	41.0
16-17	38.17293614608393	38.0	38.0	41.0	35.0	41.0
18-19	38.209912815566284	38.0	38.0	41.0	35.0	41.0
20-21	38.21442023923381	38.0	38.0	41.0	35.0	41.0
22-23	38.15940926017712	38.0	38.0	41.0	35.0	41.0
24-25	38.10226268938855	38.0	38.0	41.0	35.0	41.0
26-27	37.96171486150591	38.0	38.0	41.0	34.0	41.0
28-29	37.843548524390215	38.0	38.0	40.5	34.0	41.0
30-31	37.78451671132554	38.0	38.0	40.0	34.0	41.0
32-33	37.65632250991017	38.0	37.5	40.0	34.0	41.0
34-35	37.62839324606311	38.0	37.5	40.0	34.0	41.0
36-37	37.615665987433445	38.0	37.0	40.0	34.0	41.0
38-39	37.60435695758753	38.0	37.0	40.0	34.0	41.0
40-41	37.61168333362127	38.0	37.5	40.0	34.0	41.0
42-43	37.655399227091216	38.0	38.0	40.0	34.0	41.0
44-45	37.59780503646506	38.0	37.0	40.0	34.0	41.0
46-47	37.512646377452796	38.0	37.0	40.0	33.0	41.0
48-49	37.445927983410314	38.0	37.0	40.0	33.0	41.0
50-51	37.39893130821709	38.0	37.0	40.0	33.0	41.0
52-53	37.29665853929229	38.0	37.0	40.0	33.0	41.0
54-55	37.19884460258284	38.0	37.0	40.0	33.0	41.0
56-57	37.094639981332335	38.0	37.0	40.0	33.0	41.0
58-59	36.961937154802044	38.0	36.5	40.0	32.5	41.0
60-61	36.82635006532314	38.0	36.0	40.0	32.0	41.0
62-63	36.70761780972856	38.0	36.0	39.5	32.0	41.0
64-65	36.495668425911326	38.0	35.5	39.0	31.5	41.0
66-67	36.27241135966467	38.0	35.0	39.0	31.0	41.0
68-69	36.07953216078644	38.0	35.0	38.0	31.0	40.0
70-71	35.85469884139877	37.5	35.0	38.0	31.0	40.0
72-73	35.64601414203162	37.0	35.0	38.0	31.0	39.5
74-75	35.451496976393145	37.0	35.0	38.0	31.0	39.0
76-77	34.56470179746091	36.0	33.5	38.0	28.5	38.5
78-79	35.045618750311924	36.0	34.0	38.0	30.0	38.5
80-81	34.99830628671802	36.0	34.0	38.0	30.5	38.0
82-83	34.84758533968069	36.0	34.0	38.0	30.0	38.0
84-85	34.712660480631804	35.0	34.0	38.0	30.0	38.0
86-87	34.565868608967854	35.0	34.0	38.0	30.0	38.0
88-89	34.50905906752989	35.0	34.0	38.0	30.0	38.0
90-91	34.42500199366031	35.0	34.0	38.0	30.0	38.0
92-93	34.34940908846154	35.0	34.0	38.0	30.0	38.0
94-95	34.32666796800321	35.0	34.0	38.0	30.0	38.0
96-97	34.36718676035126	35.0	34.0	38.0	30.0	38.0
98-99	34.39367240292336	35.0	34.0	38.0	30.0	38.0
100	33.52992300053091	35.0	32.0	37.0	27.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	5.0
15	8.0
16	19.0
17	31.0
18	47.0
19	107.0
20	224.0
21	556.0
22	1435.0
23	3099.0
24	6840.0
25	12695.0
26	21096.0
27	31543.0
28	43034.0
29	57266.0
30	74363.0
31	96072.0
32	125340.0
33	163896.0
34	224776.0
35	320617.0
36	526351.0
37	1590830.0
38	733546.0
39	403819.0
40	17193.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.746142427206195	19.631997690585614	13.286046607160937	24.335813275047258
2	15.528387412344728	20.830500252648317	39.97893063428757	23.66218170071938
3	19.052713595577274	24.94495274657118	29.823253926262606	26.17907973158894
4	12.923808854655721	16.97679967872921	37.72693733895213	32.372454127662934
5	13.646757919363099	37.48726376372141	35.1548180853545	13.711160231560992
6	31.610693073485308	36.751160375225965	17.995698581016605	13.642447970272126
7	27.763300289642046	32.03288850318835	22.187079176682996	18.016732030486605
8	25.440664234987402	36.20494167089992	20.50359510362846	17.850798990484215
9	25.766559239689062	16.36355228697796	20.771193557344432	37.09869491598854
10-11	24.938252122593806	26.369738859735627	28.254959976959732	20.437049040710836
12-13	25.375218106994033	23.820707015721663	28.630958139843933	22.17311673744037
14-15	22.396695346534496	25.359100693205928	26.0644171276479	26.17978683261168
16-17	22.178524088035278	28.51556914387948	26.86814574375309	22.437761024332154
18-19	22.201715045471087	27.688246566800057	28.510234669993707	21.59980371773515
20-21	22.820748126451292	26.887231044966963	28.48714421431278	21.804876614268963
22-23	22.619629180297544	26.75268075148147	28.34186956205487	22.28582050616611
24-25	22.378803080961635	26.886854359368506	28.473219306721887	22.26112325294797
26-27	22.326796501885717	27.051584416943708	28.365213408981386	22.256405672189185
28-29	22.276102758173565	27.088057727593984	28.422502705138246	22.21333680909421
30-31	22.52429037188528	26.94807927383625	28.29863205194908	22.22899830232939
32-33	22.293830188177026	27.106902831971553	28.270924210823594	22.328342769027824
34-35	22.450434209159525	27.16912782267637	28.23745683990601	22.142981128258096
36-37	22.475103547590955	27.1260233085559	28.12844267880097	22.270430465052176
38-39	22.27743464212458	27.243712479398884	28.25211370442676	22.226739174049783
40-41	22.52777373838096	27.079100622528756	28.122153667887716	22.270971971202563
42-43	22.41398425447475	27.16890113926052	27.9690673592188	22.448047247045924
44-45	22.500356077559974	27.22562565439893	27.835120061838314	22.438898206202783
46-47	22.57403300989955	27.266255835029305	27.642966229107714	22.51674492596343
48-49	22.469005142061565	27.39724992299861	27.589945297829853	22.543799637109966
50-51	22.45271093062727	27.521961534859496	27.53493727724824	22.49039025726499
52-53	22.654384870392796	27.383549243397308	27.38559213182135	22.57647375438854
54-55	22.59836924117751	27.32551401963839	27.439201121403052	22.636915617781046
56-57	22.48973907395611	27.462447383869797	27.46790572188706	22.579907820287033
58-59	22.527270426072697	27.478729459234756	27.43081879613754	22.563181318555007
60-61	22.61514542352297	27.395724522111976	27.499072031080633	22.49005802328442
62-63	22.62618519963833	27.470095120721005	27.404909624855406	22.49881005478526
64-65	22.552725959491497	27.462452097725016	27.373655566860943	22.611166375922547
66-67	22.603486954211284	27.42135908424941	27.308458579784322	22.666695381754987
68-69	22.528513142933583	27.446846364791654	27.37703163667982	22.647608855594946
70-71	22.52497130132538	27.309650638848616	27.434456345367042	22.730921714458958
72-73	22.59855794155291	27.35424846569392	27.31383012281581	22.733363469937366
74-75	22.41254376473721	27.4669205439108	27.328717206732005	22.791818484619984
76-77	22.577010300566652	27.38690347888601	27.287186558519082	22.748899662028254
78-79	22.522530352253405	27.38889267240311	27.274484901451167	22.81409207389232
80-81	22.539764116412993	27.425930323457376	27.284085860100337	22.75021970002929
82-83	22.517447064253933	27.47649024932578	27.33378281172752	22.672279874692766
84-85	22.586831504853848	27.420217470536766	27.281909153398765	22.711041871210618
86-87	22.478156156115485	27.418924033156443	27.352547940588057	22.75037187014001
88-89	22.516027856309638	27.380953653822743	27.353429105117655	22.749589384749964
90-91	22.569064755680998	27.42880161652952	27.32246160750156	22.67967202028793
92-93	22.547061054276146	27.43766890900636	27.322733488010815	22.692536548706684
94-95	22.43642538568693	27.46420126775886	27.378517147662997	22.720856198891212
96-97	22.549574744694688	27.596228248990823	27.36190406490293	22.492292941411556
98-99	23.63158416841522	29.500952803275855	27.59895188948881	19.26851113882011
100	30.274326197380685	40.927913083801506	28.797760718817813	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	875.0
1	751.5
2	887.0
3	1241.0
4	1606.0
5	2415.0
6	3205.0
7	3974.5
8	4686.5
9	4838.0
10	4456.5
11	3926.0
12	4022.0
13	5067.5
14	7686.5
15	11268.0
16	14218.5
17	16063.0
18	16667.0
19	16473.5
20	16307.0
21	16350.5
22	17194.0
23	19454.0
24	23184.5
25	28609.5
26	35646.5
27	43977.0
28	52666.5
29	62191.5
30	71842.5
31	80987.0
32	90848.5
33	100307.0
34	108732.5
35	117203.0
36	126958.0
37	136356.5
38	144305.5
39	150733.0
40	157600.5
41	164892.0
42	170568.0
43	176176.0
44	181633.5
45	186131.0
46	189059.5
47	191768.0
48	193271.5
49	192988.5
50	192196.0
51	189347.0
52	184066.0
53	177633.5
54	169850.0
55	159901.0
56	148128.5
57	135321.0
58	121588.0
59	105870.5
60	89764.0
61	74350.5
62	59984.5
63	47355.5
64	36315.5
65	27711.5
66	21041.0
67	16116.5
68	12470.0
69	9262.0
70	6623.5
71	4710.0
72	3402.5
73	2362.5
74	1613.0
75	1090.0
76	730.5
77	479.0
78	326.0
79	229.0
80	144.5
81	90.0
82	65.0
83	45.5
84	31.5
85	22.5
86	16.5
87	11.5
88	7.5
89	6.5
90	5.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	8.97906060618985E-5
18-19	0.0
20-21	6.28553008120007E-4
22-23	3.81648113704198E-4
24-25	3.5925786755083025E-4
26-27	0.0017069597127186804
28-29	0.001146089049770827
30-31	0.0018002545559942177
32-33	8.910031353159695E-4
34-35	7.355012061654011E-4
36-37	0.004491484883765488
38-39	0.0017978417425186023
40-41	4.5010219050886825E-4
42-43	1.1633534360807088E-5
44-45	4.579377162434735E-4
46-47	0.0023379922675356244
48-49	1.1993971350240515E-4
50-51	8.844265329898366E-4
52-53	0.0019939172069305625
54-55	9.760044970333787E-4
56-57	8.743325563847698E-5
58-59	0.0012125936949675088
60-61	1.7882369771642136E-4
62-63	9.426498299937489E-4
64-65	0.0021803701406795705
66-67	0.0026688464888748077
68-69	0.0012841785457641103
70-71	0.0011514558669317996
72-73	7.817534895349952E-4
74-75	0.015395208466948196
76-77	0.005188047750285343
78-79	0.0016099464486219784
80-81	0.0037554150195801563
82-83	0.00759974667511083
84-85	0.003264682017003651
86-87	0.004980592242073033
88-89	0.0057886792983082086
90-91	2.1724163026188323E-4
92-93	1.577482883127606E-5
94-95	2.2480790565904124E-4
96-97	0.0023846291737177686
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	92.0
20-21	254.0
22-23	557.0
24-25	1191.0
26-27	1918.0
28-29	4658.0
30-31	9950.0
32-33	13259.0
34-35	18959.0
36-37	29053.0
38-39	34018.0
40-41	34209.0
42-43	37594.0
44-45	44690.0
46-47	44806.0
48-49	42635.0
50-51	39985.0
52-53	39270.0
54-55	43414.0
56-57	44692.0
58-59	44255.0
60-61	42901.0
62-63	41951.0
64-65	44463.0
66-67	46584.0
68-69	46660.0
70-71	46099.0
72-73	43900.0
74-75	45241.0
76-77	46082.0
78-79	47793.0
80-81	47558.0
82-83	45413.0
84-85	45835.0
86-87	48588.0
88-89	51098.0
90-91	52294.0
92-93	54246.0
94-95	63925.0
96-97	151924.0
98-99	948236.0
100-101	1964559.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.91118087494687
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.70081881247198	83.34840087503845
2	5.509580454371994	9.907457695562245
3	1.0973648036487642	2.959960960399938
4	0.3347603875548854	1.2039480702085845
5	0.14746913496117386	0.6629562033483026
6	0.06774938108365558	0.36548561140669567
7	0.03803490047351436	0.23938339712243265
8	0.026412912650177586	0.18998529333794312
9	0.01981327798319097	0.16032916984250573
>10	0.05617233553445913	0.7751525545647261
>50	0.0011355530389304677	0.06952138110091582
>100	6.880462271822889E-4	0.1174187880672621
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	8.97906060618985E-5	0.0	0.0	0.0
3	0.0	8.97906060618985E-5	0.0	0.0	0.0
4	0.0	2.2447651515474626E-4	0.0	0.0	0.0
5	0.0	2.693718181856955E-4	0.0	2.2447651515474624E-5	0.0
6	6.734295454642387E-5	2.693718181856955E-4	0.0	2.2447651515474624E-5	0.0
7	6.734295454642387E-5	2.693718181856955E-4	0.0	2.2447651515474624E-5	0.0
8	6.734295454642387E-5	3.1426712121664476E-4	0.0	2.2447651515474624E-5	0.0
9	6.734295454642387E-5	4.0405772727854325E-4	0.0	4.489530303094925E-5	0.0
10-11	7.856678030416118E-5	4.0405772727854325E-4	0.0	4.489530303094925E-5	0.0
12-13	8.97906060618985E-5	4.265053787940179E-4	0.0	4.489530303094925E-5	0.0
14-15	8.97906060618985E-5	5.499674621291283E-4	0.0	4.489530303094925E-5	1.0101443181963581E-4
16-17	1.3468590909284774E-4	5.611912878868657E-4	0.0	4.489530303094925E-5	1.3468590909284774E-4
18-19	1.3468590909284774E-4	6.285342424332895E-4	0.0	4.489530303094925E-5	1.3468590909284774E-4
20-21	1.3468590909284774E-4	7.407725000106626E-4	0.0	6.734295454642387E-5	1.3468590909284774E-4
22-23	1.3468590909284774E-4	7.632201515261372E-4	0.0	6.734295454642387E-5	1.4590973485058506E-4
24-25	1.4590973485058506E-4	7.632201515261372E-4	0.0	1.5713356060832238E-4	1.5713356060832238E-4
26-27	1.5713356060832238E-4	7.632201515261372E-4	0.0	2.1325268939700895E-4	1.5713356060832238E-4
28-29	1.5713356060832238E-4	7.632201515261372E-4	0.0	5.611912878868657E-4	1.683573863660597E-4
30-31	1.5713356060832238E-4	8.081154545570865E-4	0.0	0.0020202886363927166	2.0202886363927163E-4
32-33	1.5713356060832238E-4	8.081154545570865E-4	0.0	0.004568097083399086	2.693718181856955E-4
34-35	1.5713356060832238E-4	9.091298863767223E-4	0.0	0.008305631060725611	2.693718181856955E-4
36-37	1.5713356060832238E-4	9.876966666808836E-4	0.0	0.014310377841115073	2.693718181856955E-4
38-39	1.5713356060832238E-4	0.0010550396212273075	0.0	0.0231323048866966	2.693718181856955E-4
40-41	1.79581212123797E-4	0.0011336064015314686	0.0	0.03262766147774237	2.693718181856955E-4
42-43	1.79581212123797E-4	0.0011560540530469432	0.0	0.04261686640212857	2.693718181856955E-4
44-45	1.79581212123797E-4	0.0013131876136552654	0.0	0.05372845390228852	2.693718181856955E-4
46-47	1.79581212123797E-4	0.0014478735227481132	0.0	0.06423395481153064	2.693718181856955E-4
48-49	1.79581212123797E-4	0.0014590973485058506	0.0	0.07533431848593283	2.693718181856955E-4
50-51	1.79581212123797E-4	0.001582559431840961	0.0	0.08617653416790709	2.693718181856955E-4
52-53	1.79581212123797E-4	0.0015937832575986983	0.0	0.09699630219836586	3.2549094697438205E-4
54-55	1.79581212123797E-4	0.0015937832575986983	0.0	0.10741201250154608	3.816100757630686E-4
56-57	1.79581212123797E-4	0.001616230909114173	0.0	0.11675023553198352	4.0405772727854325E-4
58-59	1.79581212123797E-4	0.0016723500379028596	0.0	0.12567317700938468	4.265053787940179E-4
60-61	2.805956439434328E-4	0.0016947976894183341	0.0	0.13396758424435257	4.377292045517552E-4
62-63	2.918194697011701E-4	0.0017172453409338088	0.0	0.14244157269144425	4.938483333404418E-4
64-65	2.918194697011701E-4	0.0017958121212379701	0.0	0.14996153594912826	4.938483333404418E-4
66-67	2.918194697011701E-4	0.0018070359469957074	0.0	0.1577396471992402	5.162959848559164E-4
68-69	2.918194697011701E-4	0.0018294835985111818	0.0	0.1655626537523831	5.38743636371391E-4
70-71	2.918194697011701E-4	0.0018407074242689193	0.0	0.17219593477520584	5.38743636371391E-4
72-73	2.918194697011701E-4	0.0018407074242689193	0.0	0.1776843855707394	6.060865909178149E-4
74-75	2.918194697011701E-4	0.0018743789015421312	0.0	0.18209534909353015	6.060865909178149E-4
76-77	2.918194697011701E-4	0.0018968265530576057	0.0	0.18633795522995486	6.173104166755522E-4
78-79	2.918194697011701E-4	0.0019080503788153431	0.0	0.18900922576029636	6.285342424332895E-4
80-81	2.918194697011701E-4	0.0019080503788153431	0.0	0.18997447477546175	6.397580681910269E-4
82-83	2.918194697011701E-4	0.0019080503788153431	0.0	0.19097339526790036	6.509818939487641E-4
84-85	2.918194697011701E-4	0.0019304980303308178	0.0	0.19148969125275628	6.509818939487641E-4
86-87	2.918194697011701E-4	0.002020288636392716	0.0	0.19166927246488008	6.509818939487641E-4
88	2.918194697011701E-4	0.0021325268939700894	0.0	0.19168049629063783	6.509818939487641E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5140	0.0	46.426285	1
GTATCAA	8820	0.0	35.148857	1
TCAACGC	10605	0.0	28.215244	4
ATCAACG	10775	0.0	27.731567	3
CAACGCA	10970	0.0	27.238617	5
TATCAAC	11240	0.0	26.916613	2
AACGCAG	11265	0.0	26.635834	6
GTACATG	13130	0.0	22.28351	1
ACGCAGA	13515	0.0	22.201454	7
TACATGG	13270	0.0	21.673124	2
CGCAGAG	13895	0.0	21.564423	8
ACATGGG	13765	0.0	20.501795	3
CATGGGG	10235	0.0	18.28723	4
GCAGAGT	16330	0.0	18.221838	9
GAGTACT	9205	0.0	18.191936	12-13
GTGGTAT	2740	0.0	18.175646	1
CAGAGTA	15545	0.0	17.206453	10-11
AGAGTAC	14365	0.0	17.045338	10-11
TGGTATC	2705	0.0	16.876587	2
GTACTTT	10600	0.0	15.89676	14-15
>>END_MODULE