FastQCFastQC Report
Fri 27 May 2016
SRR2138690_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138690_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6733080
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80000.046.5398561
GTATCAA135450.034.2388731
TCAACGC164650.027.373324
ATCAACG166750.027.1020373
CAACGCA169550.026.5822325
TATCAAC173350.026.376332
AACGCAG174200.026.0367096
ACGCAGA209050.021.7157467
GTACATG216650.021.4438971
TACATGG214000.021.3851362
CGCAGAG213900.021.2424478
ACATGGG219500.020.365723
GTGGTAT40600.018.8033731
CATGGGG162800.018.1804144
GCAGAGT250150.017.9682989
GAGTACT134100.017.9463112-13
CAGAGTA239150.017.05356810-11
TGGTATC40950.016.8481792
AGAGTAC222200.016.59066810-11
GAGTACA159050.016.2989731
Produced by FastQC (version 0.11.5)