FastQCFastQC Report
Fri 27 May 2016
SRR2138689_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138689_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8960172
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102200.048.262211
GTATCAA178250.034.0693441
TCAACGC213200.027.8663714
ATCAACG216500.027.4606363
CAACGCA220450.026.9499245
AACGCAG226700.026.4975136
TATCAAC226250.026.495622
GTACATG304750.022.6158581
TACATGG298200.022.5189252
ACGCAGA271200.022.0889287
CGCAGAG280300.021.342438
ACATGGG307150.021.3131643
CATGGGG225100.019.003844
GCAGAGT325650.018.3576419
GAGTACT174750.018.1181112-13
GAGTACA214200.017.4721261
CAGAGTA315950.017.1295510-11
AGTACAT218350.016.6497922
AGAGTAC291050.016.423610-11
GTGGTAT62350.016.244261
Produced by FastQC (version 0.11.5)