##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138689_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8960172
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19038987198014	33.0	31.0	34.0	30.0	34.0
2	32.444899941652906	34.0	31.0	34.0	31.0	34.0
3	32.46652062036309	34.0	31.0	34.0	31.0	34.0
4	36.092628132584956	37.0	35.0	37.0	35.0	37.0
5	35.85076000773199	37.0	35.0	37.0	35.0	37.0
6	35.94417082618504	37.0	35.0	37.0	35.0	37.0
7	35.9041879999625	37.0	35.0	37.0	35.0	37.0
8	35.916567338216275	37.0	35.0	37.0	35.0	37.0
9	37.70625731291766	39.0	37.0	39.0	35.0	39.0
10-11	37.61828930292856	39.0	37.0	39.0	35.0	39.0
12-13	37.57986403609216	39.0	37.0	39.0	35.0	39.0
14-15	38.879649743330816	40.0	38.0	41.0	35.0	41.0
16-17	38.83812671229972	40.0	38.0	41.0	35.0	41.0
18-19	38.840071094617386	40.0	38.0	41.0	35.0	41.0
20-21	38.85593829452188	40.0	38.0	41.0	35.0	41.0
22-23	38.78103875603078	40.0	38.0	41.0	34.5	41.0
24-25	38.70807248744971	40.0	38.0	41.0	34.0	41.0
26-27	38.553455956142855	40.0	38.0	41.0	34.0	41.0
28-29	38.42770565065406	40.0	38.0	41.0	34.0	41.0
30-31	38.293952947107016	40.0	38.0	41.0	34.0	41.0
32-33	38.1429578920994	40.0	38.0	41.0	33.5	41.0
34-35	38.10358205553146	40.0	37.5	41.0	33.0	41.0
36-37	38.119745821746385	40.0	38.0	41.0	33.0	41.0
38-39	38.084976932971365	40.0	37.0	41.0	33.0	41.0
40-41	38.11936500626068	40.0	37.5	41.0	33.0	41.0
42-43	38.17593042742939	40.0	38.0	41.0	33.0	41.0
44-45	38.15781426355825	40.0	37.5	41.0	33.0	41.0
46-47	38.06121626478807	40.0	37.0	41.0	33.0	41.0
48-49	37.97773150224475	40.0	37.0	41.0	33.0	41.0
50-51	37.87690082192333	40.0	37.0	41.0	33.0	41.0
52-53	37.71221839216358	40.0	36.5	41.0	33.0	41.0
54-55	37.58243161161333	39.5	36.0	41.0	32.0	41.0
56-57	37.42140427759878	39.0	36.0	41.0	32.0	41.0
58-59	37.19301397503291	39.0	35.0	41.0	31.5	41.0
60-61	37.000752001818434	39.0	35.0	40.5	31.0	41.0
62-63	36.76874113972244	38.0	35.0	40.0	31.0	41.0
64-65	36.463503876796	38.0	35.0	40.0	31.0	41.0
66-67	36.13633405414535	37.0	35.0	40.0	31.0	41.0
68-69	35.8776729575176	37.0	34.0	39.5	30.0	41.0
70-71	35.49346458114522	36.0	34.0	39.0	30.0	41.0
72-73	35.2081525698778	36.0	34.0	39.0	30.0	40.0
74-75	34.88917438144286	35.0	34.0	38.0	29.5	40.0
76-77	33.51412624290608	34.5	32.0	36.5	28.0	39.0
78-79	34.27931406917442	35.0	33.5	37.0	29.0	39.0
80-81	34.23751400861963	35.0	34.0	36.5	30.0	39.0
82-83	34.00597123138223	35.0	34.0	36.0	29.5	37.5
84-85	33.8118225717759	35.0	33.5	36.0	29.0	37.0
86-87	33.591471999708645	35.0	33.0	35.0	29.0	37.0
88-89	33.48920476145074	35.0	33.0	35.0	29.0	36.0
90-91	33.39468022092167	35.0	33.0	35.0	29.0	36.0
92-93	33.2295941308222	35.0	33.0	35.0	29.0	36.0
94-95	33.253449091266916	35.0	33.0	35.0	29.0	35.5
96-97	33.30320043906838	35.0	33.0	35.0	29.0	35.0
98-99	33.390200537598666	35.0	33.0	35.0	30.0	35.0
100	32.20650149028813	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	14.0
20	52.0
21	272.0
22	1208.0
23	4232.0
24	11092.0
25	24253.0
26	44044.0
27	70114.0
28	101255.0
29	136898.0
30	174543.0
31	219845.0
32	274223.0
33	348563.0
34	472960.0
35	661311.0
36	1046948.0
37	1828918.0
38	2247707.0
39	1238454.0
40	53263.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.00104953342414	19.546778789514306	13.118274961686001	24.333896715375552
2	15.763614805608642	20.664067609416424	39.87839742362089	23.693920161354047
3	19.16727714601907	24.976138850905986	29.596273375109316	26.260310627965623
4	12.926593373430778	16.813940625246925	37.59924474664103	32.66022125468127
5	13.640541721743734	37.66292655989193	34.98960734235905	13.706924376005283
6	31.819076687367165	36.55484515252609	17.96965504680044	13.656423113306307
7	28.178644338523856	31.872568964077924	21.98416503611761	17.964621661280606
8	25.660065454100657	36.05095973604078	20.404050279391956	17.884924530466602
9	26.10292525634553	16.104746649952702	20.62499469876248	37.16733339493929
10-11	25.30132234068721	26.22352003957067	27.996521718556295	20.478635901185825
12-13	25.658899181846063	23.629524076100324	28.41723908871392	22.294337653339692
14-15	22.63578199168498	25.126928367000094	25.959875547031906	26.277414094283014
16-17	22.398421903620612	28.215409039735604	26.68983960949369	22.69632944715009
18-19	22.33452103374801	27.51348969640315	28.308491176285454	21.843498093563383
20-21	23.200839930212666	26.648101875579254	28.20934347583069	21.94171471837739
22-23	22.874940890547627	26.59558407952302	28.046993556379046	22.482481473550305
24-25	22.5947624068296	26.697960890648954	28.210407129693017	22.49686957282843
26-27	22.57791523226129	26.87321024488342	28.135647055335077	22.41322746752021
28-29	22.49389669724618	27.019803068366212	28.068515674200047	22.417784560187563
30-31	22.834080616118154	26.87693354559518	27.933792522755468	22.355193315531196
32-33	22.499023345246165	27.027913365057504	27.968390941939475	22.504672347756856
34-35	22.61599543200934	27.079260751573624	27.968466317852414	22.336277498564623
36-37	22.715063925962784	26.986681694931065	27.886045898836752	22.412208480269406
38-39	22.43338433865404	27.150054467706262	28.046007320108444	22.37055387353125
40-41	22.759432191908825	27.00040913684894	27.833948534116104	22.406210137126127
42-43	22.60893244353948	27.062217743821687	27.70875103117416	22.620098781464666
44-45	22.68046992569608	27.250413695481424	27.51624141073883	22.552874968083668
46-47	22.728331256002175	27.168340262452322	27.33369788012519	22.769630601420307
48-49	22.686306133015904	27.25996142745208	27.300282523888075	22.75344991564394
50-51	22.623113714946744	27.37886255727926	27.273813518709893	22.724210209064104
52-53	22.79417691586385	27.265633156790358	27.108219179930902	22.831970747414886
54-55	22.78138557321114	27.205531893764995	27.174339614126954	22.838742918896912
56-57	22.76493299874611	27.335563552531774	27.137404517930687	22.76209893079143
58-59	22.70365926544381	27.417088645064343	27.163784218385473	22.715467871106373
60-61	22.831194222622393	27.343367434018777	27.13931298028535	22.68612536307348
62-63	22.805050119786344	27.420012560399087	27.078093472522873	22.69684384729169
64-65	22.69929988665783	27.38171044927511	27.153481492309638	22.765508171757425
66-67	22.804920529301516	27.413727215624494	26.93373774702776	22.847614508046235
68-69	22.780236260227067	27.35708442496091	27.056932501287832	22.805746813524195
70-71	22.745252959047875	27.12835159835206	27.146247935222096	22.980147507377964
72-73	22.828778893543962	27.206283678409125	27.10841466397601	22.856522764070906
74-75	22.60303250958425	27.348998870905454	27.10167339869812	22.94629522081218
76-77	22.72574534288732	27.243511681477166	27.050518063952218	22.980224911683298
78-79	22.666914972832977	27.204837584975383	27.039120626885556	23.089126815306084
80-81	22.715987265687435	27.25060617102082	27.040109688298614	22.99329687499314
82-83	22.797198599269915	27.182437114027675	27.168365622231118	22.851998664471296
84-85	22.94954996270984	27.12671885630843	27.047872063767304	22.875859117214425
86-87	22.680758155483844	27.184813504161486	27.188532638503126	22.94589570185154
88-89	22.784320522470846	27.153662825451285	27.1325575598859	22.92945909219197
90-91	22.852406469208926	27.12336112463076	27.204125251323596	22.82010715483672
92-93	22.875695472217117	27.037436095997048	27.21691305761702	22.869955374168814
94-95	22.69427282906801	27.141664538249433	27.297895035200913	22.866167597481645
96-97	22.702406051680846	27.290097153209658	27.356741924205664	22.65075487090383
98-99	23.853452626535837	29.24824351069941	27.49057740297737	19.407726459787384
100	30.824448350764687	40.69085166520602	28.484699984029294	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1341.0
1	1240.5
2	1921.0
3	2896.5
4	4032.0
5	6647.0
6	8946.0
7	10719.5
8	12015.0
9	11967.5
10	10695.5
11	9243.5
12	9675.5
13	12760.0
14	20607.5
15	31040.0
16	39172.5
17	43799.0
18	44832.0
19	43086.0
20	39866.0
21	36673.0
22	35439.0
23	37956.5
24	44108.0
25	53876.5
26	68104.5
27	83271.5
28	97628.5
29	113938.5
30	130183.0
31	146547.0
32	167208.0
33	189659.5
34	210024.0
35	230938.5
36	254538.5
37	274198.5
38	288282.0
39	299798.5
40	312875.0
41	325836.0
42	335537.0
43	346522.5
44	358251.5
45	368084.0
46	374846.0
47	381025.5
48	387521.0
49	391846.5
50	392378.5
51	388658.0
52	379219.0
53	369059.5
54	356841.0
55	338623.0
56	316470.0
57	290603.0
58	262598.5
59	230230.5
60	197344.5
61	165101.0
62	133647.5
63	105155.0
64	80069.0
65	61265.0
66	46642.0
67	34930.0
68	26024.5
69	19138.0
70	13679.5
71	9440.0
72	6422.5
73	4178.0
74	2717.5
75	1838.0
76	1236.5
77	789.5
78	516.0
79	359.0
80	252.0
81	170.5
82	117.5
83	76.0
84	55.5
85	49.5
86	45.0
87	36.0
88	24.5
89	15.0
90	9.0
91	5.0
92	3.0
93	2.5
94	2.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.0602475041773753E-4
18-19	0.0
20-21	8.984502848673349E-4
22-23	4.4089471490017644E-4
24-25	7.87066257414541E-4
26-27	0.0024681335756124726
28-29	0.0018829325517915403
30-31	0.002545868720908554
32-33	0.0014304618018736357
34-35	0.0010921084860038134
36-37	0.006700243336540094
38-39	0.0030564621507628465
40-41	8.282752462177587E-4
42-43	9.28592728167131E-5
44-45	7.154367800184372E-4
46-47	0.0033589085137767476
48-49	1.8614321114092337E-4
50-51	0.001335711466409012
52-53	0.0033247160618873196
54-55	0.001611657790254764
56-57	1.1913103316388511E-4
58-59	0.0019860398530305128
60-61	3.724310604422221E-4
62-63	0.0014616988136462643
64-65	0.003529494115702338
66-67	0.0037201942726710732
68-69	0.0017128124643655772
70-71	0.0013466504530863553
72-73	0.0015516930252437596
74-75	0.023354153242823826
76-77	0.007966078163158937
78-79	0.0021420996803367008
80-81	0.005181994122096272
82-83	0.011143023078389385
84-85	0.0053667211730567075
86-87	0.007169915676588289
88-89	0.009373188806281236
90-91	4.2769875339277976E-4
92-93	8.073273677761255E-6
94-95	2.8855153641738865E-4
96-97	0.0038139390998679955
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	186.0
20-21	670.0
22-23	1314.0
24-25	2960.0
26-27	4374.0
28-29	9741.0
30-31	21195.0
32-33	28830.0
34-35	40736.0
36-37	62781.0
38-39	75315.0
40-41	77096.0
42-43	84574.0
44-45	99678.0
46-47	99647.0
48-49	94065.0
50-51	85272.0
52-53	83326.0
54-55	90320.0
56-57	94550.0
58-59	93709.0
60-61	91189.0
62-63	88569.0
64-65	92773.0
66-67	97764.0
68-69	100088.0
70-71	98238.0
72-73	92756.0
74-75	95229.0
76-77	95403.0
78-79	101087.0
80-81	101400.0
82-83	97569.0
84-85	98029.0
86-87	104243.0
88-89	112607.0
90-91	119229.0
92-93	125294.0
94-95	144928.0
96-97	325454.0
98-99	1877367.0
100-101	3750617.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.63793391997628
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.721541523167	75.93845902636215
2	7.03266922190233	11.904611857688407
3	1.7722484242940113	4.499983350755358
4	0.6915916931239228	2.3413956808890832
5	0.2992761105867389	1.2665055835833958
6	0.16890215274596587	0.8577317545832878
7	0.09375485205723022	0.55546518811679
8	0.05530009614312348	0.3744388706504016
9	0.037837088457075875	0.2882207693298856
>10	0.12307628040478041	1.6091218475491509
>50	0.002883492610184086	0.16175995912297142
>100	8.097301901002555E-4	0.10312441423068879
>500	4.3734820441610454E-5	0.02095111597148109
>1k	6.559949712196209E-5	0.07823058116697974
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.696300026383422E-5	0.0	1.116050004397237E-5	0.0
3	0.0	6.696300026383422E-5	0.0	1.116050004397237E-5	0.0
4	0.0	8.928400035177896E-5	0.0	1.116050004397237E-5	1.116050004397237E-5
5	0.0	1.116050004397237E-4	0.0	1.116050004397237E-5	1.116050004397237E-5
6	0.0	1.116050004397237E-4	0.0	1.116050004397237E-5	1.116050004397237E-5
7	0.0	1.2276550048369607E-4	0.0	1.116050004397237E-5	1.116050004397237E-5
8	0.0	1.450865005716408E-4	0.0	1.116050004397237E-5	1.116050004397237E-5
9	0.0	2.232100008794474E-4	0.0	1.116050004397237E-5	3.348150013191711E-5
10-11	0.0	2.232100008794474E-4	0.0	1.116050004397237E-5	3.348150013191711E-5
12-13	0.0	2.232100008794474E-4	0.0	1.6740750065958554E-5	3.348150013191711E-5
14-15	0.0	3.1249400123122635E-4	0.0	2.232100008794474E-5	1.0602475041773751E-4
16-17	0.0	3.1249400123122635E-4	0.0	2.232100008794474E-5	1.116050004397237E-4
18-19	0.0	3.4039525134115726E-4	0.0	2.232100008794474E-5	1.116050004397237E-4
20-21	0.0	3.682965014510882E-4	0.0	7.25432502858204E-5	1.1718525046170988E-4
22-23	0.0	3.738767514730744E-4	0.0	8.928400035177896E-5	1.5624700061561318E-4
24-25	0.0	4.0735825160499153E-4	0.0	1.2834575050568226E-4	2.1204950083547502E-4
26-27	0.0	4.2409900167095005E-4	0.0	2.3995075094540596E-4	2.1204950083547502E-4
28-29	0.0	4.2409900167095005E-4	0.0	7.421732529241626E-4	2.1204950083547502E-4
30-31	0.0	4.687410018468395E-4	0.0	0.0020646925081348882	2.1204950083547502E-4
32-33	0.0	4.687410018468395E-4	0.0	0.004620447018204561	2.3437050092341976E-4
34-35	0.0	5.357040021106737E-4	0.0	0.008348054032891333	2.4553100096739213E-4
36-37	0.0	6.138275024184804E-4	0.0	0.013626970553690264	2.4553100096739213E-4
38-39	0.0	6.249880024624527E-4	0.0	0.022973889340517126	2.4553100096739213E-4
40-41	0.0	6.473090025503975E-4	0.0	0.03280628987925678	2.5669150101136453E-4
42-43	0.0	6.807905026823145E-4	0.0	0.04186861591496235	2.5669150101136453E-4
44-45	0.0	7.812350030780659E-4	0.0	0.05232600445616446	2.5669150101136453E-4
46-47	0.0	8.481980033419001E-4	0.0	0.06359810950057655	2.5669150101136453E-4
48-49	0.0	8.481980033419001E-4	0.0	0.07487021454498864	2.6785200105533687E-4
50-51	0.0	9.151610036057344E-4	0.0	0.08564567733744397	2.6785200105533687E-4
52-53	0.0	9.263215036497067E-4	0.0	0.09610306587864609	3.01333501187254E-4
54-55	0.0	9.263215036497067E-4	0.0	0.10630934316885882	3.4039525134115726E-4
56-57	0.0	9.263215036497067E-4	0.0	0.11522658270399275	3.459755013631435E-4
58-59	0.0	9.37482003693679E-4	0.0	0.12400989623859898	3.5155575138512966E-4
60-61	0.0	9.598030037816238E-4	0.0	0.13262022202252366	3.7945700149506057E-4
62-63	0.0	9.709635038255962E-4	1.116050004397237E-5	0.14075622655457953	3.7945700149506057E-4
64-65	0.0	0.0010379265040894304	1.116050004397237E-5	0.1481277368336233	3.7945700149506057E-4
66-67	0.0	0.0010379265040894304	1.116050004397237E-5	0.15605169186484366	3.7945700149506057E-4
68-69	0.0	0.0010602475041773753	1.116050004397237E-5	0.1639142641458222	3.7945700149506057E-4
70-71	0.0	0.0010658277541993615	1.116050004397237E-5	0.1705826629220957	4.017780015830053E-4
72-73	0.0	0.0010714080042213475	1.116050004397237E-5	0.17551002369150948	4.464200017588948E-4
74-75	0.0	0.0011048895043532646	1.116050004397237E-5	0.179745433458197	4.464200017588948E-4
76-77	0.0	0.0011830130046610711	1.116050004397237E-5	0.18381901597424694	4.464200017588948E-4
78-79	0.0	0.0012164945047929883	1.116050004397237E-5	0.18627432598392085	4.464200017588948E-4
80-81	0.0	0.0012164945047929883	1.116050004397237E-5	0.18724528948774644	4.464200017588948E-4
82-83	0.0	0.001238815504880933	1.116050004397237E-5	0.18781447498998904	4.575805018028672E-4
84-85	0.0	0.0012611365049688779	1.116050004397237E-5	0.18800978374075855	4.575805018028672E-4
86-87	0.0	0.0012834575050568228	1.116050004397237E-5	0.18814370974128622	4.687410018468395E-4
88	0.0	0.0013280995052327121	1.116050004397237E-5	0.18816603074137417	4.687410018468395E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10220	0.0	48.26221	1
GTATCAA	17825	0.0	34.069344	1
TCAACGC	21320	0.0	27.866371	4
ATCAACG	21650	0.0	27.460636	3
CAACGCA	22045	0.0	26.949924	5
AACGCAG	22670	0.0	26.497513	6
TATCAAC	22625	0.0	26.49562	2
GTACATG	30475	0.0	22.615858	1
TACATGG	29820	0.0	22.518925	2
ACGCAGA	27120	0.0	22.088928	7
CGCAGAG	28030	0.0	21.34243	8
ACATGGG	30715	0.0	21.313164	3
CATGGGG	22510	0.0	19.00384	4
GCAGAGT	32565	0.0	18.357641	9
GAGTACT	17475	0.0	18.11811	12-13
GAGTACA	21420	0.0	17.472126	1
CAGAGTA	31595	0.0	17.12955	10-11
AGTACAT	21835	0.0	16.649792	2
AGAGTAC	29105	0.0	16.4236	10-11
GTGGTAT	6235	0.0	16.24426	1
>>END_MODULE