FastQCFastQC Report
Fri 27 May 2016
SRR2138688_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138688_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7796153
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85950.049.155511
GTATCAA155850.036.5220261
TCAACGC193300.028.789054
ATCAACG197500.028.1975783
TATCAAC201450.027.949812
CAACGCA201250.027.6517935
AACGCAG205650.027.2195786
GTACATG282650.023.3419041
TACATGG281600.023.0796892
ACGCAGA244100.022.8816577
ACATGGG283100.022.3494513
CGCAGAG262100.021.3102368
CATGGGG210800.019.6340624
GTGGTAT46000.019.5094811
GCAGAGT294800.018.9186489
TGGTATC46050.018.5094322
GAGTACA200150.018.3447511
GAGTACT152300.017.5701512-13
AGTACAT203350.017.3709072
CAGAGTA281900.017.21877310-11
Produced by FastQC (version 0.11.5)