##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138688_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7796153
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18307683289438	33.0	31.0	34.0	30.0	34.0
2	32.4354566925508	34.0	31.0	34.0	31.0	34.0
3	32.45500518011897	34.0	31.0	34.0	31.0	34.0
4	36.085777305807106	37.0	35.0	37.0	35.0	37.0
5	35.84039871972754	37.0	35.0	37.0	35.0	37.0
6	35.93625920373805	37.0	35.0	37.0	35.0	37.0
7	35.89368294850037	37.0	35.0	37.0	35.0	37.0
8	35.90792497273976	37.0	35.0	37.0	35.0	37.0
9	37.695563953144585	39.0	37.0	39.0	35.0	39.0
10-11	37.60754002647203	39.0	37.0	39.0	35.0	39.0
12-13	37.566454763009396	39.0	37.0	39.0	35.0	39.0
14-15	38.86124464206898	40.0	38.0	41.0	35.0	41.0
16-17	38.81836419834244	40.0	38.0	41.0	35.0	41.0
18-19	38.822693513069844	40.0	38.0	41.0	35.0	41.0
20-21	38.83897200420544	40.0	38.0	41.0	35.0	41.0
22-23	38.76080551391495	40.0	38.0	41.0	34.0	41.0
24-25	38.68526941638022	40.0	38.0	41.0	34.0	41.0
26-27	38.52921095522779	40.0	38.0	41.0	34.0	41.0
28-29	38.40179395775179	40.0	38.0	41.0	34.0	41.0
30-31	38.274150943422654	40.0	38.0	41.0	34.0	41.0
32-33	38.11624773155147	40.0	37.5	41.0	33.5	41.0
34-35	38.07237931711144	40.0	37.5	41.0	33.0	41.0
36-37	38.08637418991373	40.0	37.5	41.0	33.0	41.0
38-39	38.046953269235345	40.0	37.0	41.0	33.0	41.0
40-41	38.0797908746093	40.0	37.0	41.0	33.0	41.0
42-43	38.139735346505944	40.0	37.5	41.0	33.0	41.0
44-45	38.1171098208562	40.0	37.0	41.0	33.0	41.0
46-47	38.01520786006266	40.0	37.0	41.0	33.0	41.0
48-49	37.92538356809814	40.0	37.0	41.0	33.0	41.0
50-51	37.82329635454725	40.0	37.0	41.0	33.0	41.0
52-53	37.650084473079076	40.0	36.5	41.0	32.5	41.0
54-55	37.51446854990746	39.0	36.0	41.0	32.0	41.0
56-57	37.34871098178344	39.0	35.5	41.0	32.0	41.0
58-59	37.109353899361544	39.0	35.0	41.0	31.0	41.0
60-61	36.91090132239009	39.0	35.0	40.0	31.0	41.0
62-63	36.67159023940437	38.0	35.0	40.0	31.0	41.0
64-65	36.36033180146684	37.5	35.0	40.0	31.0	41.0
66-67	36.022607963561285	37.0	34.5	40.0	30.0	41.0
68-69	35.759242552326924	36.5	34.0	39.0	30.0	41.0
70-71	35.373663005051824	36.0	34.0	39.0	30.0	41.0
72-73	35.08936324113645	35.5	34.0	39.0	30.0	40.0
74-75	34.775860966361584	35.0	34.0	37.5	29.5	40.0
76-77	33.4069109687141	34.5	32.0	36.0	28.0	39.0
78-79	34.174282082266075	35.0	33.5	36.5	29.0	39.0
80-81	34.14212753318586	35.0	34.0	36.0	30.0	39.0
82-83	33.92152627642353	35.0	34.0	36.0	29.0	37.0
84-85	33.737545361870495	35.0	33.0	35.5	29.0	37.0
86-87	33.53317228219203	35.0	33.0	35.0	29.0	36.5
88-89	33.44206243820183	35.0	33.0	35.0	29.0	36.0
90-91	33.355590852642024	35.0	33.0	35.0	29.0	36.0
92-93	33.196795920597125	35.0	33.0	35.0	29.0	36.0
94-95	33.22361168224249	35.0	33.0	35.0	29.0	35.5
96-97	33.27833620055122	35.0	33.0	35.0	29.0	35.0
98-99	33.369934066095425	35.0	33.0	35.0	30.0	35.0
100	32.17072195589166	34.0	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	1.0
19	5.0
20	54.0
21	258.0
22	1193.0
23	3763.0
24	10088.0
25	21843.0
26	39297.0
27	62958.0
28	90697.0
29	120971.0
30	154431.0
31	193049.0
32	241377.0
33	306788.0
34	415755.0
35	584382.0
36	930441.0
37	1611380.0
38	1921396.0
39	1040755.0
40	45270.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.04465292048527	19.439805760610394	13.172137591450554	24.343403727453783
2	15.934564136953187	20.822590321149416	39.44501858801386	23.79782695388354
3	19.2082300078	24.921573499134766	29.578832021382855	26.29136447168238
4	13.198535226284042	16.78149466794713	37.2335304348183	32.78643967095053
5	13.942620161507863	37.40888615192647	34.728846393856045	13.919647292709621
6	32.04888359682012	36.269850014487915	17.791749340989075	13.889517047702887
7	28.290093845002783	31.80930389642174	21.883472528053257	18.01712973052222
8	25.951337794422454	35.61306454606522	20.330411678683063	18.105185980829262
9	26.352574147788015	15.964707208799005	20.539335233672297	37.14338340974068
10-11	25.45187992077631	26.085282061550103	27.768823931495447	20.694014086178143
12-13	25.76397615593229	23.54799860905757	28.112262547951534	22.575762687058603
14-15	22.832575245765444	24.91719954700735	25.722269688652855	26.527955518574352
16-17	22.661430999095	28.03254379522791	26.418193720341492	22.887831485335596
18-19	22.682905273921637	27.311803654956492	28.010795837382872	21.994495233739002
20-21	23.52573027937663	26.450503616289687	27.90905807658554	22.114708027748136
22-23	23.202217760855113	26.277353909416718	27.78092613542445	22.73950219430372
24-25	22.913265020035524	26.450928329548297	27.96525960982148	22.670547040594695
26-27	22.87639359333768	26.620410276856717	27.871744805623166	22.63145132418244
28-29	22.822869922035814	26.66036607649212	27.862153876982422	22.654610124489643
30-31	23.145902924110455	26.496617025433277	27.679730193151737	22.677749857304534
32-33	22.86361706109311	26.67108374224397	27.672337712628742	22.79296148403418
34-35	23.01231710095174	26.7218616076765	27.684402634137406	22.58141865723435
36-37	23.114311401833493	26.57957369183884	27.577258137572773	22.7288567687549
38-39	22.799074169933288	26.885277176235366	27.705984027284636	22.609664626546707
40-41	23.112861163606063	26.68388898493643	27.530100969152844	22.673148882304663
42-43	22.906065417592885	26.781013187143788	27.329391118316593	22.983530276946734
44-45	22.912693114467906	26.87102354739817	27.279666122269457	22.936617215864462
46-47	23.06534235794587	26.744320595863652	27.11492202616798	23.075415020022493
48-49	22.974434382746118	26.983477165560288	26.99469197847864	23.047396473214953
50-51	22.973282006326944	27.021421252878174	26.979867060213575	23.025429680581308
52-53	23.21622681294366	26.97745110750433	26.76189621741211	23.044425862139896
54-55	23.191872589266055	26.832522871460263	26.87217265343299	23.1034318858407
56-57	23.14036452906455	27.02305706561991	26.763635288981664	23.07294311633388
58-59	23.059357650150332	27.061303480452164	26.797190894487983	23.082147974909525
60-61	23.14592879724123	27.019740311945707	26.83318536102892	23.001145529784147
62-63	23.179724582161874	27.063662266836886	26.77503187101443	22.981581279986806
64-65	23.10631414843862	27.000268318433935	26.813311938802116	23.08010559432533
66-67	23.2267436062835	27.039999581102197	26.600041381558842	23.133215431055465
68-69	23.1116140238342	26.988400892348324	26.752161656138046	23.147823427679423
70-71	23.132459619213435	26.764088400720336	26.791042832531602	23.312409147534627
72-73	23.19202967636811	26.863993906020983	26.70614720182229	23.23782921578862
74-75	22.959143621156702	26.98221539354133	26.73560355065736	23.323037434644608
76-77	23.111804005381945	26.889523831202773	26.670034917978462	23.32863724543682
78-79	22.992863378519527	26.928500347398405	26.693607994890044	23.385028279192028
80-81	23.147392461275306	26.86531808339765	26.63735215020543	23.349937305121614
82-83	23.222526369789584	26.826531296945678	26.79556838844012	23.155373944824614
84-85	23.23958322390422	26.812925241531936	26.6742128982782	23.273278636285642
86-87	23.017982569632107	26.914730626033155	26.784202939918494	23.283083864416245
88-89	23.17127703450721	26.76917288336722	26.774599683074896	23.284950399050672
90-91	23.151839551155966	26.75138075737824	26.831282733968703	23.265496957497085
92-93	23.279743478186937	26.748097868133573	26.805001878480578	23.167156775198908
94-95	23.04795899416499	26.772585290054092	26.90349990234192	23.275955813439
96-97	23.023600095732103	26.897492008502148	26.978157362406822	23.100750533358923
98-99	24.207177564496867	28.904337109685624	27.09560430857821	19.792881017239303
100	31.376022496805284	40.552579655345745	28.07139784784897	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1340.0
1	1199.5
2	1735.0
3	2602.5
4	3607.5
5	5792.0
6	7744.0
7	9417.5
8	10735.0
9	10816.0
10	9719.5
11	8407.0
12	8904.5
13	11811.5
14	18692.5
15	27750.5
16	34946.5
17	39197.0
18	40080.0
19	37978.0
20	34419.0
21	31053.0
22	29502.0
23	31041.5
24	35765.0
25	43839.5
26	55801.0
27	69088.0
28	81123.0
29	95214.0
30	108774.5
31	121009.0
32	137010.5
33	154715.0
34	171666.5
35	189157.0
36	208965.0
37	224526.5
38	236399.5
39	248272.0
40	261839.0
41	274599.0
42	282790.0
43	293725.5
44	305961.0
45	316317.0
46	326052.0
47	336323.0
48	344982.5
49	349430.0
50	348642.0
51	345855.5
52	337820.5
53	325689.5
54	314392.5
55	301068.5
56	283702.5
57	261843.0
58	238094.0
59	211420.0
60	184896.0
61	158269.5
62	130622.5
63	105965.5
64	84692.0
65	68053.0
66	55246.5
67	41252.0
68	29211.0
69	22085.0
70	15783.0
71	10416.5
72	7131.0
73	4903.0
74	3348.0
75	2325.0
76	1534.5
77	954.5
78	643.0
79	439.5
80	287.0
81	189.0
82	141.0
83	110.5
84	77.5
85	48.0
86	35.0
87	26.5
88	17.0
89	11.0
90	7.5
91	4.5
92	2.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.6033548854159225E-4
18-19	0.0
20-21	9.107424314578132E-4
22-23	5.131422464302778E-4
24-25	7.24966505585097E-4
26-27	0.0024068113145290985
28-29	0.001971705448785529
30-31	0.0023865098766129365
32-33	0.0013804514527800131
34-35	0.0010490023987188184
36-37	0.006427521200074864
38-39	0.0030197476396897514
40-41	7.948699096034194E-4
42-43	4.0112968816980306E-5
44-45	6.149943045445039E-4
46-47	0.003222874278343024
48-49	2.701522682088344E-4
50-51	0.001506325551421996
52-53	0.003044767930974686
54-55	0.0016392221039294014
56-57	2.0281432399341985E-4
58-59	0.0018771601089984749
60-61	4.082148563933535E-4
62-63	0.0013143287899365374
64-65	0.003139151120331111
66-67	0.004111805694850887
68-69	0.0018108276723504018
70-71	0.0018760882499247189
72-73	0.0014204316330606185
74-75	0.023740361933569928
76-77	0.008560101810783155
78-79	0.002233673727362302
80-81	0.0052657017919268126
82-83	0.011090218496276756
84-85	0.0048671700277568754
86-87	0.007335475292118632
88-89	0.009785371177578078
90-91	3.685077004290351E-4
92-93	5.636847773261932E-5
94-95	4.990854738581427E-4
96-97	0.0033963206000271703
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	198.0
20-21	587.0
22-23	1223.0
24-25	2881.0
26-27	4237.0
28-29	9409.0
30-31	20455.0
32-33	27117.0
34-35	38522.0
36-37	58009.0
38-39	67840.0
40-41	68956.0
42-43	76190.0
44-45	90185.0
46-47	90816.0
48-49	83729.0
50-51	76225.0
52-53	74653.0
54-55	80858.0
56-57	84422.0
58-59	82556.0
60-61	80438.0
62-63	77999.0
64-65	83246.0
66-67	87370.0
68-69	89566.0
70-71	87366.0
72-73	82657.0
74-75	84597.0
76-77	84986.0
78-79	89141.0
80-81	89982.0
82-83	86470.0
84-85	87135.0
86-87	93229.0
88-89	99170.0
90-91	105110.0
92-93	109538.0
94-95	127870.0
96-97	283996.0
98-99	1628187.0
100-101	3199032.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.19800120068044
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.02073374524579	78.45805316659512
2	6.282224341878163	10.830303627283156
3	1.4839808984805558	3.837485618070414
4	0.5269044098113582	1.8167242779825308
5	0.2550131652628136	1.0990812562756662
6	0.12689399461425457	0.6562805220071193
7	0.0857722712202548	0.5175378836340028
8	0.04891533426787519	0.3373123233563193
9	0.031704594212236384	0.24595853849760946
>10	0.13316927995417957	1.800401229761642
>50	0.0035143705362276428	0.19920902148601616
>100	0.001099362189175111	0.15679784042758485
>500	7.42323271534053E-5	0.04485469462267844
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.282683908332738E-5	0.0
2	0.0	7.696103449996428E-5	0.0	1.282683908332738E-5	0.0
3	0.0	7.696103449996428E-5	0.0	3.848051724998214E-5	0.0
4	0.0	1.924025862499107E-4	0.0	3.848051724998214E-5	0.0
5	0.0	1.924025862499107E-4	0.0	3.848051724998214E-5	0.0
6	5.130735633330952E-5	1.924025862499107E-4	0.0	5.130735633330952E-5	0.0
7	5.130735633330952E-5	1.924025862499107E-4	0.0	5.130735633330952E-5	0.0
8	5.130735633330952E-5	2.0522942533323808E-4	0.0	5.130735633330952E-5	0.0
9	5.130735633330952E-5	5.77207758749732E-4	0.0	8.978787358329166E-5	1.282683908332738E-5
10-11	5.130735633330952E-5	5.77207758749732E-4	0.0	1.0902813220828273E-4	1.282683908332738E-5
12-13	5.130735633330952E-5	5.77207758749732E-4	0.0	1.346818103749375E-4	1.282683908332738E-5
14-15	5.130735633330952E-5	8.337445404162796E-4	0.0	1.4109522991660118E-4	3.848051724998214E-5
16-17	5.130735633330952E-5	8.46571379499607E-4	0.0	2.3088310349989282E-4	3.848051724998214E-5
18-19	5.130735633330952E-5	9.171189944579076E-4	0.0	2.629502012082113E-4	5.130735633330952E-5
20-21	5.130735633330952E-5	0.0010197337071245266	0.0	2.821904598332023E-4	6.41341954166369E-5
22-23	6.41341954166369E-5	0.0011287618393328095	0.0	3.0784413799985714E-4	7.054761495830059E-5
24-25	6.41341954166369E-5	0.001218549712916101	0.0	3.3349781616651185E-4	8.337445404162796E-5
26-27	6.41341954166369E-5	0.0012634436497077467	0.0	5.002467242497678E-4	8.978787358329166E-5
28-29	6.41341954166369E-5	0.0012762704887910744	0.0	0.0010261471266661904	9.620129312495535E-5
30-31	6.41341954166369E-5	0.0014366059773326665	0.0	0.002558954397123812	1.1544155174994641E-4
32-33	6.41341954166369E-5	0.0015007401727493033	0.0	0.005714356811622348	1.1544155174994641E-4
34-35	7.696103449996428E-5	0.00173803669579086	0.0	0.009722744025162154	1.1544155174994641E-4
36-37	7.696103449996428E-5	0.0019496795406657617	0.0	0.016020722015075897	1.1544155174994641E-4
38-39	7.696103449996428E-5	0.0019753332188324163	0.0	0.026269366442654474	1.1544155174994641E-4
40-41	7.696103449996428E-5	0.0021677358050823273	0.0	0.03746719696239927	1.1544155174994641E-4
42-43	8.978787358329166E-5	0.0022446968395822917	0.0	0.04756833274051959	1.1544155174994641E-4
44-45	9.620129312495535E-5	0.0025717812362071393	0.0	0.058419838605014554	1.1544155174994641E-4
46-47	1.0261471266661904E-4	0.0028219045983320233	0.0	0.07067588334913386	1.1544155174994641E-4
48-49	1.0261471266661904E-4	0.0028219045983320233	0.0	0.08236754717358677	1.1544155174994641E-4
50-51	1.0261471266661904E-4	0.003116921897248553	0.0	0.0938411547336231	1.1544155174994641E-4
52-53	1.0261471266661904E-4	0.003142575575415208	0.0	0.10537248306953442	1.282683908332738E-4
54-55	1.0261471266661904E-4	0.003155402414498535	0.0	0.11608289370411279	1.4109522991660118E-4
56-57	1.0261471266661904E-4	0.00318105609266519	0.0	0.1261263087063581	1.5392206899992857E-4
58-59	1.1544155174994641E-4	0.003187469512206854	0.0	0.1354963146567288	1.6674890808325593E-4
60-61	1.1544155174994641E-4	0.003225950029456836	0.0	0.1444622751759746	1.6674890808325593E-4
62-63	1.1544155174994641E-4	0.0032836708053318093	0.0	0.15295364264913736	1.795757471665833E-4
64-65	1.1544155174994641E-4	0.003694129655998285	0.0	0.1610666183693419	1.795757471665833E-4
66-67	1.282683908332738E-4	0.0037518504318732587	0.0	0.16914752699183816	1.795757471665833E-4
68-69	1.282683908332738E-4	0.0038737054031648685	0.0	0.17699113909129285	1.795757471665833E-4
70-71	1.282683908332738E-4	0.003963493276748161	0.0	0.1844691862768727	1.795757471665833E-4
72-73	1.282683908332738E-4	0.0039891469549148155	0.0	0.1899141794677452	1.924025862499107E-4
74-75	1.282683908332738E-4	0.00413665560437308	0.0	0.19426889133653483	1.924025862499107E-4
76-77	1.282683908332738E-4	0.0042969910929146725	0.0	0.19867491056165779	1.924025862499107E-4
78-79	1.282683908332738E-4	0.004348298449247981	0.0	0.2015352956772398	1.924025862499107E-4
80-81	1.282683908332738E-4	0.004348298449247981	0.0	0.20252937570619767	1.988160057915744E-4
82-83	1.282683908332738E-4	0.004354711868789645	0.0	0.20306168952815573	2.3088310349989282E-4
84-85	1.6674890808325593E-4	0.0043739521274146365	0.0	0.20328615921211396	2.3088310349989282E-4
86-87	1.6674890808325593E-4	0.004438086322831273	0.0	0.20340160076386393	2.3088310349989282E-4
88	1.6674890808325593E-4	0.004617662069997856	0.0	0.20342084102248892	2.3088310349989282E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8595	0.0	49.15551	1
GTATCAA	15585	0.0	36.522026	1
TCAACGC	19330	0.0	28.78905	4
ATCAACG	19750	0.0	28.197578	3
TATCAAC	20145	0.0	27.94981	2
CAACGCA	20125	0.0	27.651793	5
AACGCAG	20565	0.0	27.219578	6
GTACATG	28265	0.0	23.341904	1
TACATGG	28160	0.0	23.079689	2
ACGCAGA	24410	0.0	22.881657	7
ACATGGG	28310	0.0	22.349451	3
CGCAGAG	26210	0.0	21.310236	8
CATGGGG	21080	0.0	19.634062	4
GTGGTAT	4600	0.0	19.509481	1
GCAGAGT	29480	0.0	18.918648	9
TGGTATC	4605	0.0	18.509432	2
GAGTACA	20015	0.0	18.344751	1
GAGTACT	15230	0.0	17.57015	12-13
AGTACAT	20335	0.0	17.370907	2
CAGAGTA	28190	0.0	17.218773	10-11
>>END_MODULE