FastQCFastQC Report
Fri 27 May 2016
SRR2138687_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138687_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16067893
Sequences flagged as poor quality0
Sequence length19-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA198050.049.267361
GTATCAA372500.036.4853941
TCAACGC479300.027.8801634
ATCAACG488300.027.358113
CAACGCA491300.027.1910535
TATCAAC499550.026.9580462
AACGCAG501450.026.8479296
GTACATG689950.024.425831
TACATGG695350.023.9084742
ACATGGG710300.023.090113
ACGCAGA599450.022.4387427
CGCAGAG619900.021.7307458
CATGGGG523700.020.7154144
GCAGAGT704800.018.9826229
AGAGTAC638600.017.20727710-11
GAGTACT350250.017.19893312-13
CAGAGTA680750.016.75546810-11
GAGTACA487350.016.5518511
ATGGGGG324500.016.4819185
AGTACAT482450.016.2808322
Produced by FastQC (version 0.11.5)