##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138687_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16067893
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03177784417658	33.0	31.0	34.0	30.0	34.0
2	32.2994538238461	34.0	31.0	34.0	30.0	34.0
3	32.313995805174955	34.0	31.0	34.0	30.0	34.0
4	35.991662877018165	37.0	35.0	37.0	35.0	37.0
5	35.73007574795277	37.0	35.0	37.0	35.0	37.0
6	35.82469985330373	37.0	35.0	37.0	35.0	37.0
7	35.77486699718501	37.0	35.0	37.0	33.0	37.0
8	35.78884624138336	37.0	35.0	37.0	33.0	37.0
9	37.55838217244788	39.0	37.0	39.0	35.0	39.0
10-11	37.45617197600208	39.0	37.0	39.0	34.5	39.0
12-13	37.41064179976802	39.0	37.0	39.0	34.0	39.0
14-15	38.64575523374471	40.0	38.0	41.0	34.0	41.0
16-17	38.59638037171395	40.0	38.0	41.0	34.0	41.0
18-19	38.60434423480415	40.0	38.0	41.0	34.0	41.0
20-21	38.622457741072665	40.0	38.0	41.0	34.0	41.0
22-23	38.54009849621983	40.0	38.0	41.0	34.0	41.0
24-25	38.45350680084819	40.0	38.0	41.0	34.0	41.0
26-27	38.277979182018996	40.0	38.0	41.0	34.0	41.0
28-29	38.143638528293266	40.0	38.0	41.0	33.0	41.0
30-31	37.99583085095205	40.0	37.0	41.0	33.0	41.0
32-33	37.8311273597993	40.0	37.0	41.0	33.0	41.0
34-35	37.785024837297755	40.0	37.0	41.0	33.0	41.0
36-37	37.796946461088666	40.0	37.0	41.0	33.0	41.0
38-39	37.749226398609764	40.0	37.0	41.0	33.0	41.0
40-41	37.786744699515694	40.0	37.0	41.0	33.0	41.0
42-43	37.84365411242682	40.0	37.0	41.0	33.0	41.0
44-45	37.81483584313558	40.0	37.0	41.0	33.0	41.0
46-47	37.70228064563415	40.0	37.0	41.0	33.0	41.0
48-49	37.60068243771181	40.0	36.0	41.0	32.0	41.0
50-51	37.48564584850277	39.5	36.0	41.0	32.0	41.0
52-53	37.294379986975244	39.0	36.0	41.0	31.5	41.0
54-55	37.14020788877859	39.0	35.0	41.0	31.0	41.0
56-57	36.95563780376732	39.0	35.0	40.5	31.0	41.0
58-59	36.69784884831212	38.0	35.0	40.0	31.0	41.0
60-61	36.48685821080171	38.0	35.0	40.0	31.0	41.0
62-63	36.233152249237605	37.5	35.0	40.0	30.5	41.0
64-65	35.9022704017878	37.0	34.0	40.0	30.0	41.0
66-67	35.55829605314896	36.5	34.0	39.0	30.0	41.0
68-69	35.29335378217044	36.0	34.0	39.0	29.0	41.0
70-71	34.90984875734604	35.5	34.0	39.0	29.0	40.0
72-73	34.631631194261715	35.0	34.0	37.5	29.0	40.0
74-75	34.326467404036684	35.0	33.0	37.0	29.0	39.0
76-77	32.982177132612264	34.0	31.5	36.0	27.0	38.5
78-79	33.79901383365659	35.0	33.0	36.0	29.0	39.0
80-81	33.80257334894349	35.0	33.0	36.0	29.0	37.5
82-83	33.604726817868375	35.0	33.0	35.5	29.0	37.0
84-85	33.44086622035366	35.0	33.0	35.0	29.0	37.0
86-87	33.248593849354364	35.0	33.0	35.0	29.0	36.0
88-89	33.1758771243967	35.0	33.0	35.0	29.0	36.0
90-91	33.103025226015056	35.0	33.0	35.0	29.0	36.0
92-93	32.95418022033383	35.0	33.0	35.0	29.0	35.5
94-95	32.99273193690526	35.0	33.0	35.0	29.0	35.0
96-97	33.06241549023826	34.0	33.0	35.0	29.0	35.0
98-99	33.17404804400003	34.0	33.0	35.0	29.0	35.0
100	31.917419322247948	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	0.0
13	0.0
14	2.0
15	0.0
16	1.0
17	0.0
18	7.0
19	27.0
20	149.0
21	661.0
22	2895.0
23	9851.0
24	25245.0
25	53278.0
26	95359.0
27	149039.0
28	213428.0
29	282022.0
30	358375.0
31	445346.0
32	552774.0
33	701969.0
34	942673.0
35	1317905.0
36	2069554.0
37	3349060.0
38	3492576.0
39	1932246.0
40	73449.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.02619515825752	19.70731943509955	13.09452956899825	24.171955837644674
2	15.986744497240554	21.116216046497197	39.157386721457506	23.739652734804746
3	18.849005280281613	25.966354144877613	29.683468765942116	25.501171808898654
4	13.208153676402997	16.969766975670055	37.355121794749316	32.466957553177636
5	13.715824470576198	37.91874267522194	34.52971711972441	13.835715734477445
6	31.396854584480987	36.46292018499252	18.11945723064001	14.020767999886482
7	28.09914778496471	31.951942921203173	22.26964667987271	17.679262613959406
8	26.286041362112634	35.0813451396521	20.626120674316166	18.006492823919103
9	27.00195974668241	15.534301852769369	20.439213778682745	37.02452462186548
10-11	25.793356975927082	25.857998307556567	27.665267001715783	20.683377714800567
12-13	26.14079207522729	23.427125759425955	28.069439471622072	22.362642693724684
14-15	23.26819079514657	24.497966223698402	25.75030217091936	26.483540810235667
16-17	22.82981858046813	27.521262544640724	26.3543808784831	23.29453799640805
18-19	22.868016982687152	26.899049551798733	27.972348334657195	22.26058513085692
20-21	23.871795680901528	25.993842822816287	27.774590105011466	22.359771391270723
22-23	23.623628944638664	25.890784895835118	27.5975864010355	22.887999758490714
24-25	23.209582363824563	26.11172102950905	27.751039112931693	22.927657493734692
26-27	23.125066662306853	26.230143585743104	27.748494891623633	22.89629486032641
28-29	23.081791868550315	26.306135819015353	27.705799782736346	22.906272529697986
30-31	23.41928924463804	26.260552440232143	27.433870500414137	22.88628781471568
32-33	23.0981259791025	26.466263634314497	27.473291283138863	22.96231910344414
34-35	23.183247698395988	26.507298778020004	27.485129906275713	22.82432361730829
36-37	23.39802903350848	26.283352324637487	27.379620111073034	22.938998530781003
38-39	23.092192844361808	26.512057577878995	27.50334405008767	22.89240552767153
40-41	23.39960575009823	26.327538819640374	27.39259283033993	22.880262599921465
42-43	23.269272022783827	26.436383091137106	27.136538217810287	23.15780666826878
44-45	23.27490479820235	26.599261880149843	26.973576986789354	23.15225633485845
46-47	23.39436155591478	26.463092114136806	26.73625403434355	23.406292295604867
48-49	23.326021678436906	26.587974078296554	26.64594486178459	23.44005938148195
50-51	23.171826091392443	26.737617029856725	26.58524479492789	23.505312083822943
52-53	23.47813233129323	26.609947834250054	26.388261348918547	23.523658485538167
54-55	23.464370186253593	26.525193997599583	26.467209750181965	23.543226065964863
56-57	23.441222138685124	26.748751492611405	26.398956032956907	23.41107033574657
58-59	23.351054627787796	26.828028560966892	26.421513448370888	23.399403362874423
60-61	23.499348028629374	26.754874608916435	26.392281976977717	23.35349538547647
62-63	23.48317809385394	26.743998507144575	26.349640638467015	23.423182760534473
64-65	23.421136131477148	26.736934298329018	26.416682349357163	23.425247220836667
66-67	23.53052201041676	26.726370685653418	26.11126280788647	23.631844496043357
68-69	23.419207593243723	26.700146470027335	26.3167724940019	23.563873442727047
70-71	23.414691958836112	26.387822862144045	26.482703650501872	23.71478152851797
72-73	23.564925836863047	26.473317248062518	26.35789927850894	23.603857636565497
74-75	23.187286020167086	26.737510425777277	26.398151167703677	23.677052386351964
76-77	23.390500655035186	26.518555557019823	26.38017789518964	23.710765892755347
78-79	23.341130913017192	26.532916612617264	26.278446510383652	23.847505963981895
80-81	23.441262264987966	26.532607713935963	26.270116690453005	23.756013330623066
82-83	23.45801549440907	26.429735705320688	26.431351277580706	23.680897522689527
84-85	23.634951303475923	26.416700931830682	26.304952870402786	23.643394894290605
86-87	23.284746330143744	26.48543630533856	26.503592085450116	23.72622527906758
88-89	23.461659988300955	26.368466811789464	26.458370098463412	23.711503101446162
90-91	23.572250076265846	26.361887808805456	26.478443355129343	23.58741875979936
92-93	23.62249139967077	26.369287206651233	26.351217937924176	23.657003455753824
94-95	23.344274916403904	26.340020175657042	26.545991419513175	23.76971348842588
96-97	23.385121151590813	26.468980778993778	26.61833004291526	23.527568026500145
98-99	24.540608112588558	28.502328844001994	26.680258810248255	20.276804233161194
100	32.401464954746366	40.014952641549144	27.583582403704483	0.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1978.0
1	2347.0
2	5168.5
3	8116.0
4	11106.5
5	18229.5
6	24118.5
7	27965.5
8	30346.5
9	28980.0
10	24798.5
11	20585.5
12	21173.0
13	27900.5
14	45441.0
15	69125.0
16	87088.5
17	96005.5
18	95539.5
19	87485.0
20	75950.5
21	65268.5
22	59711.0
23	61530.5
24	71413.0
25	89422.0
26	116446.0
27	143663.5
28	164148.5
29	190267.0
30	215993.0
31	237791.0
32	270350.5
33	310137.5
34	345844.5
35	384281.5
36	431286.5
37	466316.5
38	488030.0
39	502826.0
40	519941.5
41	537995.5
42	554335.5
43	580301.0
44	607686.0
45	633181.0
46	653892.0
47	674731.0
48	696823.0
49	715905.0
50	731895.5
51	742822.0
52	742198.5
53	738372.0
54	732005.0
55	709897.0
56	671421.5
57	623588.5
58	570495.5
59	506710.0
60	438386.0
61	368539.0
62	299829.5
63	237225.5
64	182400.5
65	138066.5
66	101983.0
67	76003.0
68	56985.0
69	40444.5
70	28013.0
71	18539.0
72	12172.5
73	7981.5
74	5191.0
75	3509.0
76	2437.0
77	1634.0
78	1068.5
79	717.5
80	495.0
81	324.0
82	224.5
83	153.0
84	98.5
85	63.5
86	47.5
87	41.0
88	33.0
89	23.0
90	14.5
91	8.5
92	5.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.5870157960349874E-4
18-19	0.0
20-21	8.993450807302247E-4
22-23	5.321890169818404E-4
24-25	7.159589682047603E-4
26-27	0.002410295211649611
28-29	0.0019851138895988106
30-31	0.0027128546667250235
32-33	0.001349658121577141
34-35	0.0010438574835212003
36-37	0.006506452316229018
38-39	0.0032502337741816753
40-41	8.29836557070766E-4
42-43	5.8781097527343737E-5
44-45	6.283689255824004E-4
46-47	0.0033492206534691932
48-49	2.3159650711597295E-4
50-51	0.0013755373538400838
52-53	0.0029282719437116076
54-55	0.0016925535697640244
56-57	1.1515175832236844E-4
58-59	0.0017825447374896044
60-61	3.265319329120849E-4
62-63	0.0012510647239088568
64-65	0.0032925873419022695
66-67	0.0042756338423292255
68-69	0.0018870475699821264
70-71	0.0016150002508874767
72-73	0.0011648749255399687
74-75	0.022716301314205333
76-77	0.008041570187534146
78-79	0.002376040505947964
80-81	0.005844272337312312
82-83	0.01119617946551965
84-85	0.004848694599176776
86-87	0.007290481625880163
88-89	0.009542872333744607
90-91	3.813881059436295E-4
92-93	1.9774845585646388E-5
94-95	4.361736896378724E-4
96-97	0.0034091821372052846
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	406.0
20-21	1251.0
22-23	2488.0
24-25	5832.0
26-27	9268.0
28-29	21494.0
30-31	46580.0
32-33	59408.0
34-35	83640.0
36-37	133728.0
38-39	174792.0
40-41	174103.0
42-43	183888.0
44-45	215912.0
46-47	219670.0
48-49	217545.0
50-51	197797.0
52-53	182151.0
54-55	191735.0
56-57	205369.0
58-59	211145.0
60-61	211639.0
62-63	194764.0
64-65	196308.0
66-67	208171.0
68-69	217327.0
70-71	218191.0
72-73	199216.0
74-75	197539.0
76-77	196477.0
78-79	213872.0
80-81	219506.0
82-83	204160.0
84-85	199622.0
86-87	208584.0
88-89	226558.0
90-91	242629.0
92-93	250780.0
94-95	279995.0
96-97	569655.0
98-99	3135948.0
100-101	5938750.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.36314825914151
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.72170807192387	72.18677487660214
2	7.289653735859929	11.862183553371525
3	1.99864435947455	4.878479920116742
4	0.8205566298064237	2.6705228290384624
5	0.4126505806867904	1.6787275187820083
6	0.23882236550192204	1.1658803719159059
7	0.1376304245482577	0.7838631246241937
8	0.09259780840798122	0.6027239371176126
9	0.05737645548662151	0.42014961489078184
>10	0.22054327300747184	2.8093056930642253
>50	0.006929027758670203	0.3855917393419608
>100	0.002755735190356784	0.3918197416315542
>500	1.644456800748916E-5	0.00830886713232394
>1k	1.1508777908851132E-4	0.15566821237050057
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.335387035499925E-5	0.0	0.0	0.0
3	0.0	1.120246444259991E-4	0.0	0.0	0.0
4	0.0	2.5516724563699793E-4	0.0	6.22359135699995E-6	0.0
5	0.0	2.6761442835099785E-4	0.0	6.22359135699995E-6	0.0
6	0.0	2.6761442835099785E-4	0.0	6.22359135699995E-6	0.0
7	1.24471827139999E-5	2.6761442835099785E-4	0.0	6.22359135699995E-6	0.0
8	1.24471827139999E-5	3.236267505639974E-4	0.0	6.22359135699995E-6	0.0
9	6.845950492699945E-5	6.970422319839944E-4	0.0	3.111795678499975E-5	6.22359135699995E-6
10-11	9.024207467649929E-5	6.970422319839944E-4	0.0	1.0580105306899916E-4	6.22359135699995E-6
12-13	1.120246444259991E-4	6.970422319839944E-4	0.0	1.4314260121099887E-4	6.22359135699995E-6
14-15	1.3069541849699894E-4	9.397622949069925E-4	0.0	1.4314260121099887E-4	6.22359135699995E-6
16-17	1.369190098539989E-4	9.771038430489922E-4	0.0	1.493661925679988E-4	6.22359135699995E-6
18-19	1.7114876231749863E-4	0.0011015756701889911	0.0	1.8048414935299857E-4	6.22359135699995E-6
20-21	2.3338467588749812E-4	0.0012571654541139899	0.0	2.0537851478099836E-4	6.22359135699995E-6
22-23	2.613908369939979E-4	0.0014189788293959887	0.0	2.240492888519982E-4	6.22359135699995E-6
24-25	2.6761442835099785E-4	0.0015465624522144877	0.0	2.4272006292299806E-4	6.22359135699995E-6
26-27	2.987323851359976E-4	0.0016119101614629871	0.0	3.454093203134972E-4	6.22359135699995E-6
28-29	3.1429136352849747E-4	0.0016119101614629871	0.0	9.055325424434928E-4	6.22359135699995E-6
30-31	3.3918572895649726E-4	0.0018577420200644851	0.0	0.00247387756440748	6.22359135699995E-6
32-33	3.4540932031349727E-4	0.0018919717725279848	0.0	0.005582561447228955	6.22359135699995E-6
34-35	3.547447073489972E-4	0.0022218221144489824	0.0	0.009459858862639925	6.22359135699995E-6
36-37	3.8586266413399693E-4	0.0025516724563699796	0.0	0.01615955495845037	6.22359135699995E-6
38-39	4.0764523388349676E-4	0.002626355552653979	0.0	0.02755806252879578	6.22359135699995E-6
40-41	4.387631906684965E-4	0.002869075615576977	0.0	0.040605821808746174	6.22359135699995E-6
42-43	4.636575560964963E-4	0.0029686530772889764	0.0	0.05258312337529258	6.22359135699995E-6
44-45	4.7921653448899617E-4	0.0033731865154939733	0.0	0.06541616875342648	6.22359135699995E-6
46-47	4.7921653448899617E-4	0.0036781424919869704	0.0	0.07882178453640437	6.22359135699995E-6
48-49	5.10334491273996E-4	0.0036812542876654706	0.0	0.09313293286182575	6.22359135699995E-6
50-51	5.694586091654955E-4	0.003976874877122968	0.0	0.10769302484152714	6.22359135699995E-6
52-53	5.819057918794953E-4	0.0040142164252649675	0.0	0.12149383867567452	6.22359135699995E-6
54-55	5.881293832364952E-4	0.0040546697690854675	0.0	0.13390679163721092	6.22359135699995E-6
56-57	5.974647702719953E-4	0.004144911843761967	0.0	0.14397967424851535	6.22359135699995E-6
58-59	6.036883616289952E-4	0.004154247230797467	0.0	0.15465935701712724	6.22359135699995E-6
60-61	6.099119529859951E-4	0.004225818531402966	0.0	0.16550396495669967	6.22359135699995E-6
62-63	6.410299097709949E-4	0.004306725219043966	0.0	0.17588802713585408	6.22359135699995E-6
64-65	6.721478665559946E-4	0.004757935592426462	0.0	0.185466134234277	6.22359135699995E-6
66-67	6.845950492699945E-4	0.004804612527603962	0.0	0.19480774486113395	6.22359135699995E-6
68-69	6.845950492699945E-4	0.00491974896770846	0.0	0.20411823753120586	6.22359135699995E-6
70-71	6.845950492699945E-4	0.00500065565534946	0.0	0.2122680304131973	6.22359135699995E-6
72-73	7.312719844474941E-4	0.00502555002077746	0.0	0.21873123003744177	6.22359135699995E-6
74-75	7.406073714829941E-4	0.005159357234952959	0.0	0.2234642712644402	6.22359135699995E-6
76-77	7.406073714829941E-4	0.005302499836163958	0.0	0.22776788468780568	6.22359135699995E-6
78-79	7.46830962839994E-4	0.005367847545412457	0.0	0.23097925782801765	6.22359135699995E-6
80-81	7.530545541969939E-4	0.005374071136769457	0.0	0.23209950427227766	6.22359135699995E-6
82-83	7.530545541969939E-4	0.005383406523804956	0.0	0.23291790653572314	6.22359135699995E-6
84-85	7.686135325894938E-4	0.005395853706518957	0.0	0.23321352712518062	6.22359135699995E-6
86-87	7.717253282679939E-4	0.005433195254660956	0.0	0.23332243997392815	6.22359135699995E-6
88	7.717253282679939E-4	0.005619902995370955	0.0	0.23332866356528514	6.22359135699995E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	19805	0.0	49.26736	1
GTATCAA	37250	0.0	36.485394	1
TCAACGC	47930	0.0	27.880163	4
ATCAACG	48830	0.0	27.35811	3
CAACGCA	49130	0.0	27.191053	5
TATCAAC	49955	0.0	26.958046	2
AACGCAG	50145	0.0	26.847929	6
GTACATG	68995	0.0	24.42583	1
TACATGG	69535	0.0	23.908474	2
ACATGGG	71030	0.0	23.09011	3
ACGCAGA	59945	0.0	22.438742	7
CGCAGAG	61990	0.0	21.730745	8
CATGGGG	52370	0.0	20.715414	4
GCAGAGT	70480	0.0	18.982622	9
AGAGTAC	63860	0.0	17.207277	10-11
GAGTACT	35025	0.0	17.198933	12-13
CAGAGTA	68075	0.0	16.755468	10-11
GAGTACA	48735	0.0	16.551851	1
ATGGGGG	32450	0.0	16.481918	5
AGTACAT	48245	0.0	16.280832	2
>>END_MODULE