FastQCFastQC Report
Fri 27 May 2016
SRR2138686_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138686_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7388150
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76200.049.316541
GTATCAA142100.038.9046861
TCAACGC167000.032.2873424
ATCAACG171050.031.4735363
CAACGCA171400.031.4336685
AACGCAG177500.030.4253396
TATCAAC180450.030.3063142
ACGCAGA206600.026.0986777
CGCAGAG212300.025.2776858
GTACATG199050.023.219591
TACATGG203100.022.106982
ACATGGG200600.021.4697843
GCAGAGT251500.021.4562329
CATGGGG142400.019.425654
GAGTACT152350.018.93910212-13
CAGAGTA247200.018.47240410-11
AGAGTAC225700.018.28994410-11
GTGGTAT45000.017.590871
TGGTATC40200.017.573932
GTACTTT172250.016.61552414-15
Produced by FastQC (version 0.11.5)