FastQCFastQC Report
Fri 27 May 2016
SRR2138685_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138685_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6476383
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83650.047.9800951
GTATCAA160400.038.485271
TCAACGC181050.032.923584
ATCAACG181700.032.80583
CAACGCA184700.032.227015
TATCAAC192650.031.2719732
AACGCAG190800.031.196696
ACGCAGA217500.027.3863247
CGCAGAG221400.026.750618
GTACATG211650.023.3530481
TACATGG211450.022.853462
GCAGAGT262100.022.596669
ACATGGG210700.022.2498443
GAGTACT152300.019.84757812-13
GTGGTAT43800.019.3727741
CAGAGTA258350.019.22149510-11
CATGGGG147550.019.2073084
TGGTATC41350.019.0841732
AGAGTAC242350.018.48609210-11
GTACTTT173250.017.4601214-15
Produced by FastQC (version 0.11.5)